Mobile element type:
Transposon
Name:
Tn4401d
Synonyms:
Accession:
Tn4401d-JN974188
Family:
Tn3
Group:
Tn3000
First isolate:
Yes
Partial:
ND
Evidence of transposition:
No
Host Organism:
Klebsiella pneumoniae KP529-1
Date of Isolation:
Country:
Molecular Source:
plasmid pKP529-1
| Name | Coordinates | Direction | Length | Sequence |
|---|---|---|---|---|
| IRL | 1-40 | + | 40 | GGGGTTCTAA GCGGGAATCC CAGAAAATTC CGTCATTCCG |
| IRR | 4626-4665 | - | 40 | GGGGGGGTAA GCGGGAACCC CAGAAAATTC CGCCATTCCG |
ORF Summary
| Name | Associated TE | Coordinates | Orientation | Class | Subclass |
|---|---|---|---|---|---|
| tnpR | Tn4401d | 53-1768 | - | Accessory Gene | Resolvase |
| tnpA_p | Tn4401d | 1878-2413 | + | Transposase | |
| bla KPC-3_p (ARO:3002313) | Tn4401d | 2414-2742 | + | Passenger Gene | Antibiotic Resistance |
| tnp | ISKpn6 | 2992-4311 | - | Transposase |
ORF Details
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpR | TnpR | Tn4401d | 571 | 53-1768 | - |
|---|
| Class: | Accessory Gene | ||||||||||
| Subclass: | Resolvase | ||||||||||
| Sequence Family: | Serine Site-Specific Recombinase | ||||||||||
| Transposase Chemistry: | Serine | ||||||||||
| Protein Sequence: | MKTGQTTASS STPVGFPDAD SLAALRGWYA GLSSRAAVEH YLAARKAAGQ SARGMLGHIR RQLIVFANSR HRPDLADVFQ HAPEERQGRA GAVERAIRLL PTLPAPKPQI SDSIDQWLSP RTVRALQAYG IHTLSALTVR IPRRRGWWKA IEGLGMTGAR QVEAFFAAHP QLTERARALI PIIQPSGIVP WEQLRLPNEV DGSQGNFRAP RNACTLNATN DYEAVQAWLA LHETVATQRA YRKEAARLIL WAIVERGRAL SSLTTDDAIA YRAFVRHPTP RERWVGPPRP RDSVEWRPFS GGLSARSAAY ALTVLSALFR WLIEQRYVLA NPFAGVKVRG HALRPALDTA RGFTEGEWLL LRAIADGLEW SYGWSEPAAQ RLRFMLDFGY ATGLRASELV GAVLGNVRLD EHGDHWLHLV GKGGKPGKVA LPPLAYTALD QYLAQRQLPV SRERWNPKTP LVGSLGEDGN AGITGPHLWC VLRRFFTQAA EAIEADHPVA AEKLRRATPH WMRHSHASHA LARGAQLTAV RDNLRHASIA TTSMYLHGDE LERARQMRQA FGARHVKRGV D |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpA_p | N/A | Tn4401d | 178 | 1878-2413 | + |
|---|
| Class: | Transposase | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Comment: | fragmentconsisting of N-terminal amino acids 1 to 178 | ||||||||||
| Protein Sequence: | MSKKNKLLSV LSDAEQEALY GLPEFDDAQQ LEYLAVTETE LALANSRPSL HAKVYCLLQI GYFKAKHAFF SFDWDEVEDD CAFVLSRYFH GEVFEPKAIT KHERYTQREQ IAKLFGYWPW SAAFLPQLEQ QAARTVRRDV TPGFVAAELI VWLNEHKIIR PGYTTLQELV SETLSAER |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | bla KPC-3_p (ARO:3002313) | Bla KPC-3_p | Tn4401d | 109 | 2414-2742 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antibiotic Resistance | ||||||||||
| Function: | antibiotic inactivation (ARO:0001004) | ||||||||||
| Sequence Family: | KPC beta-lactamase (ARO:3000059) | ||||||||||
| Target: | cephalosporin (ARO:0000032)||monobactam (ARO:0000004)||penam (ARO:3000008)||carbapenem (ARO:0000020) | ||||||||||
| Comment: | fragment of KPC-3 missing 553bp from 5'-end | ||||||||||
| Protein Sequence: | *RKAYKN*HW ALHWLRRSGS SLLIG*RETR PATTASARRC RQTGQSETKP EPAECMARQM TMPSSGPLGA HLLCWPSTPG RLTRMTSTAR PSSPLRLDSR SRDWASTGS |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnp | Tnp | ISKpn6 | 439 | 2992-4311 | - |
|---|
| Class: | Transposase | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Protein Sequence: | MAASYLPYRP DQSYLLPPSL GEWLPEGHLA YFISETVDTL DLSAFHARYA GGGPGNQPFH PAMMVKVLIY GYASGVFSSR KLARKLYEDV ALRVLAAGNF PAHRTLSDFR ALHLTELENL FVQVVQLARE CGLVKLGTIA VDGTKVKANA SRHKAMSYKR MKPAEDELHC EIKALLDRAK ATDDQERNEP ELDIPAEISR REKRLEAIQA AKARLEARQR EADQARGRSE DDGRRPRHPD GSDKGGGSYK REFGVPDDRD QESFTDPDSR IMKHAGGGSE QSYNGYTAVD AEHQIIVAAE LTNCAADSQA LLGMLAAVQA NTGEMPAQTL ADAGFRSEAV LAKVADHHGD VIVALGREGR EDAKVNAKTH PHTAAIAAKL KTEQGDAAYR RRKSIVEAPN GWIKAVMGLR QFSMRGLDKV QAEWKLVCMA LNLRRMAYL |
||||||||||
| TnCentral Accession | TE Name | Type | Coordinates | Strand | Length |
|---|---|---|---|---|---|
| ISKpn6-KT378596 | ISKpn6 | insertion sequence | 2824-4363 | + | 1540 |
| Name | Associated TE | Coordinates | Direction | Length | Sequence |
|---|---|---|---|---|---|
| IRR | ISKpn6 | 2824-2839 | + | 16 | GGAGTCTGCG CGGCAG |
| IRL | ISKpn6 | 4348-4363 | - | 16 | GGAGCCTGCG CGGCAG |
1. Chen L, Chavda KD, Mediavilla JR, Jacobs MR, Levi MH, Bonomo RA, Kreiswirth BN.
Partial excision of blaKPC from Tn4401 in carbapenem-resistant Klebsiella pneumoniae.. Antimicrob Agents Chemother. 2012 Mar;56(3):1635-8. doi: 10.1128/AAC.06182-11. Epub 2011 Dec 27. PubMed ID:
22203593
.