Mobile element type:
Transposon
Name:
Tn4401b
Synonyms:
Accession:
Tn4401b-KT378597
Family:
Tn3
Group:
Tn3000
First isolate:
Yes
Partial:
ND
Evidence of transposition:
ND
Host Organism:
K. pneumoniae
Date of Isolation:
2008
Country:
Columbia usa
Molecular Source:
plasmid
| Name | Coordinates | Direction | Length | Sequence |
|---|---|---|---|---|
| IRL | 1-40 | + | 40 | GGGGTTCTAA GCGGGAATCC CAGAAAATTC CGTCATTCCG |
| IR 3'-end | 4919-4924 | - | 6 | AACCGC |
| IR 5'-end | 6884-6915 | - | 32 | GGGGCGCGAA GATAGCACCG AGGGTACGCC GG |
| IRR | 9967-10006 | - | 40 | GGGGGGGTAA GCGGGAACCC CAGAAAATTC CGCCATTCCG |
ORF Summary
| Name | Associated TE | Coordinates | Orientation | Class | Subclass |
|---|---|---|---|---|---|
| tnpR | Tn4401b | 53-1768 | - | Accessory Gene | Resolvase |
| tnpA | Tn4401b | 1932-4907 | + | Transposase | |
| istA | ISKpn7 | 5014-6039 | + | Transposase | |
| istB | ISKpn7 | 6036-6815 | + | Accessory Gene | ATPase Transposition Helper |
| bla KPC-3 (ARO:3002313) | Tn4401b | 7202-8083 | + | Passenger Gene | Antibiotic Resistance |
| tnp | ISKpn6 | 8333-9652 | - | Transposase |
ORF Details
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpR | TnpR | Tn4401b | 571 | 53-1768 | - |
|---|
| Class: | Accessory Gene | ||||||||||
| Subclass: | Resolvase | ||||||||||
| Sequence Family: | Serine Site-Specific Recombinase | ||||||||||
| Transposase Chemistry: | Serine | ||||||||||
| Protein Sequence: | MKTGQTTASS STPVGFPDAD SLAALRGWYA GLSSRAAVEH YLAARKAAGQ SARGMLGHIR RQLIVFANSR HRPDLADVFQ HAPEERQGRA GAVERAIRLL PTLPAPKPQI SDSIDQWLSP RTVRALQAYG IHTLSALTVR IPRRRGWWKA IEGLGMTGAR QVEAFFAAHP QLTERARALI PIIQPSGIVP WEQLRLPNEV DGSQGNFRAP RNACTLNATN DYEAVQAWLA LHETVATQRA YRKEAARLIL WAIVERGRAL SSLTTDDAIA YRAFVRHPTP RERWVGPPRP RDSVEWRPFS GGLSARSAAY ALTVLSALFR WLIEQRYVLA NPFAGVKVRG HALRPALDTA RGFTEGEWLL LRAIADGLEW SYGWSEPAAQ RLRFMLDFGY ATGLRASELV GAVLGNVRLD EHGDHWLHLV GKGGKPGKVA LPPLAYTALD QYLAQRQLPV SRERWNPKTP LVGSLGEDGN AGITGPHLWC VLRRFFTQAA EAIEADHPVA AEKLRRATPH WMRHSHASHA LARGAQLTAV RDNLRHASIA TTSMYLHGDE LERARQMRQA FGARHVKRGV D |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpA | TnpA | Tn4401b | 991 | 1932-4907 | + |
|---|
| Class: | Transposase | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Protein Sequence: | MYGLPEFDDA QQLEYLAVTE TELALANSRP SLHAKVYCLL QIGYFKAKHA FFSFDWDEVE DDCAFVLSRY FHGEVFEPKA ITKHERYTQR EQIAKLFGYW PWSAAFLPQL EQQAARTVRR DVTPGFVAAE LIVWLNEHKI IRPGYTTLQE LVSETLSAER RRLGNLLEQA LDESAKAALS QLLVRDDTLS QLAALKQDAK NFGWRQMVRE REKRATLEPL HAIAKTLLPK LDVSQQNLLY YASLANFYTV HDLRNLKADQ TQLYLLCYAW VRYRQLSDNL VDAMAYHMKQ LEDESSAGAK QSFADEQVRR QQETPRVGRL LSLYVDDSVA DPTPFGEVRQ RAYKIMPRDT LQTTAQRMSV KPASKLALQW QAVDGLADRM RRHLRPLYVA LDLSGSNPDS PWLAALAWAK GVFAKQQRLS QRPLDEWPAA TLPKRLRPYL LTFDADGKPT GLHADRYEFW LYRQIRKRLQ SGELYLDDSL QHRHFSDELV EMDRKIDVLA KMEIPFLQQP VHAQLDALTA ELRTQWLAFN RELKQGKLTH LEYNKDTQKL TWRKPKSENE KAREKAFYEK LPFCDVADVF RFVNDECKFL SAFTPMQPRY AKKVADADSL MAVIIAQAMN HGNHVMARTS DIPYHVLDSG YQQYLRQATL HAANDCISNA IATLPIFPYY SFDLETLYGA VDGQKFSVER PTVKARYSRK YFGRGKGVVA YTLLCNHIPL NGYLIGAHDY EAHHVFDIWY RNTSDIMPTA ITGDMHSVNK ANFAILHWFG RRFEPRFTDL NAQLKELYCA DDPAQYQACL IRPVGKIDCD LIHREKPNID RIVATLGLKE MTQGTLIRKL CTYTTTNPTR RAIFEFDKLI RSIYTLRYLR DPQLERNVHR SQNRLESYHQ LRSAIAQVGG KKELTGRTDI EIEISNQCAR LIANTIIFYN SAILSRLVTK YEAAGNSKAL ALITKISPAA WRHILLNGHY TFQSSGKTID LDAIVAGLEL E |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | istA | IstA | ISKpn7 | 341 | 5014-6039 | + |
|---|
| Class: | Transposase | ||||||||||
| Function: | Transposase | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Protein Sequence: | MLTQEQTVEI RVLARQGHSI RHIARTLGVS RNTVRRYLRD PSVARYQPRE PRPTKLGPFE SYLRQRVEQA HPIWLPATVL NREIRAQGYG GGLSLLRAFL ATLKPARREA GPAVRFETEP GRQLQADFVV LRRARSPMSA FVATLGYSRM TFVTFVPDES FESVRDSLLL AFDYLGGVPR EVLFDNMKTV VLERDAYGDG KHRFHPGLLQ LADDLGFRIR LCRPYRAQTK GKVERFNRYF RESFYNPLLT RMKGTGLLLD CAAANRRVRD WLADEANVRV HATLNERPID RWRQEREHLQ PLPSRVRRDE APLLDNSLRP VPLESLQHPL SVYDAIGEAC R |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | istB | IstB | ISKpn7 | 259 | 6036-6815 | + |
|---|
| Class: | Accessory Gene | ||||||||||
| Subclass: | ATPase Transposition Helper | ||||||||||
| Comment: | ATPase | ||||||||||
| Protein Sequence: | MNLQHERIDA LCQQLKLERV ATHYPVLAQQ AVREDFSFTD FLEHLLRHEA GCRQQRSREL LTRMACFPGI KTLEDYDFSF NPGVPKALVQ ELGSLAFVER AENVVLIGPS GIGKTHLAIA LGYKAAQAGV KTRFITAADL MLQLSTAQRQ GRLKEYFNRA ILGPRLLVID EIGYLPFGRE EANLFFQVVA KRYEKGAMIL TSNLPFSQWA DAFAGDTTLT AAMLDRLLHH AHILTLSGES YRLKDKRKAG VVRKNSKPE |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | bla KPC-3 (ARO:3002313) | Bla KPC-3 | Tn4401b | 293 | 7202-8083 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antibiotic Resistance | ||||||||||
| Function: | antibiotic inactivation (ARO:0001004) | ||||||||||
| Sequence Family: | KPC beta-lactamase (ARO:3000059) | ||||||||||
| Target: | cephalosporin (ARO:0000032)||monobactam (ARO:0000004)||penam (ARO:3000008)||carbapenem (ARO:0000020) | ||||||||||
| Comment: | perfect match to ARO:3002313 reference sequence | ||||||||||
| Protein Sequence: | MSLYRRLVLL SCLSWPLAGF SATALTNLVA EPFAKLEQDF GGSIGVYAMD TGSGATVSYR AEERFPLCSS FKGFLAAAVL ARSQQQAGLL DTPIRYGKNA LVPWSPISEK YLTTGMTVAE LSAAAVQYSD NAAANLLLKE LGGPAGLTAF MRSIGDTTFR LDRWELELNS AIPGDARDTS SPRAVTESLQ KLTLGSALAA PQRQQFVDWL KGNTTGNHRI RAAVPADWAV GDKTGTCGVY GTANDYAVVW PTGRAPIVLA VYTRAPNKDD KYSEAVIAAA ARLALEGLGV NGQ |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnp | Tnp | ISKpn6 | 439 | 8333-9652 | - |
|---|
| Class: | Transposase | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Protein Sequence: | MAASYLPYRP DQSYLLPPSL GEWLPEGHLA YFISETVDTL DLSAFHARYA GGGPGNQPFH PAMMVKVLIY GYASGVFSSR KLARKLYEDV ALRVLAAGNF PAHRTLSDFR ALHLTELENL FVQVVQLARE CGLVKLGTIA VDGTKVKANA SRHKAMSYKR MKPAEDELHC EIKALLDRAK ATDDQERNEP ELDIPAEISR REKRLEAIQA AKARLEARQR EADQARGRSE DDGRRPRHPD GSDKGGGSYK REFGVPDDRD QESFTDPDSR IMKHAGGGSE QSYNGYTAVD AEHQIIVAAE LTNCAADSQA LLGMLAAVQA NTGEMPAQTL ADAGFRSEAV LAKVADHHGD VIVALGREGR EDAKVNAKTH PHTAAIAAKL KTEQGDAAYR RRKSIVEAPN GWIKAVMGLR QFSMRGLDKV QAEWKLVCMA LNLRRMAYL |
||||||||||
| TnCentral Accession | TE Name | Type | Coordinates | Strand | Length |
|---|---|---|---|---|---|
| ISKpn7-KT378596 | ISKpn7 | insertion sequence | 4928-6883 | + | 1956 |
| ISKpn6-KT378596 | ISKpn6 | insertion sequence | 8165-9704 | + | 1540 |
| Name | Associated TE | Coordinates | Direction | Length | Sequence |
|---|---|---|---|---|---|
| IRL | ISKpn7 | 4928-4956 | + | 29 | TGTTAGCAGC AGTGTAAAAC TGACCCACC |
| IRR | ISKpn7 | 6855-6883 | - | 29 | TGTCAACACC AACTGAAAAC TGACCCACT |
| IRR | ISKpn6 | 8165-8180 | + | 16 | GGAGTCTGCG CGGCAG |
| IRL | ISKpn6 | 9689-9704 | - | 16 | GGAGCCTGCG CGGCAG |