Mobile element type:
Transposon
Name:
Tn4352
Synonyms:
Accession:
Tn4352-M20306
Family:
Compound Transposon
Group:
IS26
First isolate:
Yes
Partial:
ND
Evidence of transposition:
ND
Host Organism:
Salmonella typhimurium
Date of Isolation:
1987
Country:
Molecular Source:
plasmid NTP16
ORF Summary
| Name | Associated TE | Coordinates | Orientation | Class | Subclass |
|---|---|---|---|---|---|
| tnpA | IS26 | 88-792 | + | Transposase | |
| APH(3')-Ia (ARO:3002641) | Tn4352 | 982-1797 | - | Passenger Gene | Antibiotic Resistance |
| tnpA | IS26 | 1948-2652 | + | Transposase |
ORF Details
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpA | TnpA | IS26 | 234 | 88-792 | + |
|---|
| Class: | Transposase | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Protein Sequence: | MNPFKGRHFQ RDIILWAVRW YCKYGISYRE LQEMLAERGV NVDHSTIYRW VQRYAPEMEK RLRWYWRNPS DLCPWHMDET YVKVNGRWAY LYRAVDSRGR TVDFYLSSRR NSKAAYRFLG KILNNVKKWQ IPRFINTDKA PAYGRALALL KREGRCPSDV EHRQIKYRNN VIECDHGKLK RIIGATLGFK SMKTAYATIK GIEVMRALRK GQASAFYYGD PLGEMRLVSR VFEM |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | APH(3')-Ia (ARO:3002641) | APH(3')-Ia | Tn4352 | 271 | 982-1797 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antibiotic Resistance | ||||||||||
| Function: | antibiotic inactivation (ARO:0001004) | ||||||||||
| Sequence Family: | APH(3') (ARO:3000126) | ||||||||||
| Target: | aminoglycoside antibiotic (ARO:0000016) | ||||||||||
| Comment: | strict match to reference sequence for ARO:3002641 (bitscore: 560)||Synonyms: aphA-1,apha1-1AB, APH(3')-Ic, apha7 | ||||||||||
| Protein Sequence: | MSHIQRETSC SRPRLNSNMD ADLYGYKWAR DNVGQSGATI YRLYGKPDAP ELFLKHGKGS VANDVTDEMV RLNWLTEFMP LPTIKHFIRT PDDAWLLTTA IPGKTAFQVL EEYPDSGENI VDALAVFLRR LHSIPVCNCP FNSDRVFRLA QAQSRMNNGL VDASDFDDER NGWPVEQVWK EMHKLLPFSP DSVVTHGDFS LDNLIFDEGK LIGCIDVGRV GIADRYQDLA ILWNCLGEFS PSLQKRLFQK YGIDNPDMNK LQFHLMLDEF F |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpA | TnpA | IS26 | 234 | 1948-2652 | + |
|---|
| Class: | Transposase | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Protein Sequence: | MNPFKGRHFQ RDIILWAVRW YCKYGISYRE LQEMLAERGV NVDHSTIYRW VQRYAPEMEK RLRWYWRNPS DLCPWHMDET YVKVNGRWAY LYRAVDSRGR TVDFYLSSRR NSKAAYRFLG KILNNVKKWQ IPRFINTDKA PAYGRALALL KREGRCPSDV EHRQIKYRNN VIECDHGKLK RIIGATLGFK SMKTAYATIK GIEVMRALRK GQASAFYYGD PLGEMRLVSR VFEM |
||||||||||
| TnCentral Accession | TE Name | Type | Coordinates | Strand | Length |
|---|---|---|---|---|---|
| IS26-MH257753 | IS26 | insertion sequence | 25-844 | + | 820 |
| IS26-MH257753 | IS26 | insertion sequence | 1885-2704 | + | 820 |
| Name | Associated TE | Coordinates | Direction | Length | Sequence |
|---|---|---|---|---|---|
| IR | IS26 | 25-38 | + | 14 | GGCACTGTTG CAAA |
| IR | IS26 | 831-844 | - | 14 | GGCACTGTTG CAAA |
| IR | IS26 | 1885-1898 | + | 14 | GGCACTGTTG CAAA |
| IR | IS26 | 2691-2704 | - | 14 | GGCACTGTTG CAAA |
1. Wrighton CJ, Strike P.
A pathway for the evolution of the plasmid NTP16 involving the novel kanamycin resistance transposon Tn4352.. Plasmid. 1987 Jan;17(1):37-45. doi: 10.1016/0147-619x(87)90006-0. PubMed ID:
3033719
.
2. Anderson ES, Threlfall EJ, Carr JM, McConnell MM, Smith HR. Clonal distribution of resistance plasmid-carrying Salmonella typhimurium, mainly in the Middle East.. J Hyg (Lond). 1977 Dec;79(3):425-48. doi: 10.1017/s0022172400053286. PubMed ID: 336792 .
3. Lambert CM, Wrighton CJ, Strike P. Characterization of the drug resistance plasmid NTP16.. Plasmid. 1987 Jan;17(1):26-36. doi: 10.1016/0147-619x(87)90005-9. PubMed ID: 3033718 .
2. Anderson ES, Threlfall EJ, Carr JM, McConnell MM, Smith HR. Clonal distribution of resistance plasmid-carrying Salmonella typhimurium, mainly in the Middle East.. J Hyg (Lond). 1977 Dec;79(3):425-48. doi: 10.1017/s0022172400053286. PubMed ID: 336792 .
3. Lambert CM, Wrighton CJ, Strike P. Characterization of the drug resistance plasmid NTP16.. Plasmid. 1987 Jan;17(1):26-36. doi: 10.1016/0147-619x(87)90005-9. PubMed ID: 3033718 .