Mobile element type:
Transposon
Name:
Tn402.10
Synonyms:
Tn5090
Accession:
Tn402.10-CP040126
Family:
Tn402
Group:
First isolate:
Yes
Partial:
ND
Evidence of transposition:
ND
Host Organism:
Pseudomonas aeruginosa PA298
Date of Isolation:
2019
Country:
China
Molecular Source:
plasmid pBM908
| Name | Coordinates | Direction | Length | Sequence |
|---|---|---|---|---|
| IRt | 1-33 | + | 33 | TGTCATTTTC AGAAGACGAC TGCACCAGTT GAT |
| repeat t1 | 9-27 | + | 19 | TCAGAAGACG ACTGCACCA |
| repeat t2 | 49-67 | - | 19 | TCAGGAGCTG GCTGCACAA |
| repeat t3 | 78-97 | + | 20 | TCAGAAGTGA TCTGCACCAA |
| repeat t4 | 110-128 | + | 19 | TCAATACTCG TGTGCACCA |
| repeat i4 | 8106-8124 | - | 19 | TCAGCGGACG CAGGGAGGA |
| repeat i3 | 8134-8152 | - | 19 | TCAGGCAACG ACGGGCTGC |
| repeat i2 | 8176-8194 | - | 19 | TCAGAAGCCG ACTGCACTA |
| IRi | 8193-8225 | - | 33 | TGTCGTTTTC AGAAGACGGC TGCACTGAAC GTC |
| repeat i1 | 8199-8217 | - | 19 | TCAGAAGACG GCTGCACTG |
| Name | Associated TE | Coordinates | Orientation | Length | Sequence |
|---|---|---|---|---|---|
| r5 | Tn402.10 | 3870-3883 | + | 14 | AATTTCTGTC ACAT |
| r4 | Tn402.10 | 3875-3888 | - | 14 | AGGTAATGTG ACAG |
| r3 | Tn402.10 | 3926-3939 | + | 14 | GGGTTAAGTG ACAA |
| res | Tn402.10 | 3967-4001 | + | 35 | ACACTGTCAC ATAATCGAAC GTATACGTGA CGGGT |
| r2 | Tn402.10 | 3970-3983 | - | 14 | CGATTATGTG ACAG |
| r1 | Tn402.10 | 3986-3999 | + | 14 | CGTATACGTG ACGG |
| attC qacE | Tn402.10 | 4728-4868 , 5315-5320 | + | 147 | GTTCAACGCC GAGTTCAGCG GCAGTTTTTA AGTTGTGATT TTATGGAATA CTTTTGCGCA GCAAAACCAT AAAGCCGCGA CTTAAAAACT GTCCAGCGCA GGCACGAAGT GCTGGAGCGG TGCTGCAACG ACTTGTTAGA TATCTAA |
| attC qacE core | Tn402.10 | 4728-4868 | + | 141 | GTTCAACGCC GAGTTCAGCG GCAGTTTTTA AGTTGTGATT TTATGGAATA CTTTTGCGCA GCAAAACCAT AAAGCCGCGA CTTAAAAACT GTCCAGCGCA GGCACGAAGT GCTGGAGCGG TGCTGCAACG ACTTGTTAGA T |
| attC AAC(6')-Ib10 | Tn402.10 | 5321-5386 , 5954-5960 | + | 73 | CGTTTGACAT GAGGGGCGGC CAAGGGCGCC AGCCCTTGGA CGTCCCCCTC GATGGAAGGG TTAGGCGCCT AAC |
| attC AAC(6')-Ib core | Tn402.10 | 5321-5386 | + | 66 | CGTTTGACAT GAGGGGCGGC CAAGGGCGCC AGCCCTTGGA CGTCCCCCTC GATGGAAGGG TTAGGC |
| attC qacH_sul1 | Tn402.10 | 5960-6034 , 6868-6874 | + | 82 | CGCCTGAGTT GAGGGGCTTT TGCGGAGTAG GCGCAGCCTG CGTAGCAAAA GTCCCGCTCC AACGATTTGT TATGCGCATA AC |
| attC bla VIM-1 core | Tn402.10 | 5960-6034 | + | 75 | CGCCTGAGTT GAGGGGCTTT TGCGGAGTAG GCGCAGCCTG CGTAGCAAAA GTCCCGCTCC AACGATTTGT TATGC |
| attI | Tn402.10 | 6874-6929 | + | 56 | CTTTGTTTTA GGGCGACTGC CCTGCTGCGT AACATCGTTG CTGCTCCATA ACATCA |
ORF Summary
| Name | Associated TE | Coordinates | Orientation | Class | Subclass |
|---|---|---|---|---|---|
| tniA | Tn402.10 | 142-1821 | + | Transposase | |
| tniB | Tn402.10 | 1824-2732 | + | Accessory Gene | |
| tniQ | Tn402.10 | 2729-3946 | + | Accessory Gene | Target Site Selection |
| tniR | Tn402.10 | 4008-4631 | + | Accessory Gene | Resolvase |
| qacE (ARO:3005009) | Tn402.10 | 4880-5212 | - | Passenger Gene | Antibiotic Resistance |
| AAC(6')-Ib4 (ARO:3002577) | Tn402.10 | 5381-5935 | - | Passenger Gene | Antibiotic Resistance |
| bla VIM-1 (ARO:3002271) | Tn402.10 | 6043-6843 | - | Passenger Gene | Antibiotic Resistance |
| intI1 | Tn402.10 | 7010-8023 | + | Integron Integrase | Class 1 |
ORF Details
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tniA | TniA | Tn402.10 | 559 | 142-1821 | + |
|---|
| Class: | Transposase | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Comment: | can be extended upstream by 12 amino acids| identical to tniA (Tn1721 and In2)| 25% amino acid sequence identity to TnsB from Tn7 | ||||||||||
| Protein Sequence: | MATDTPRIPE QGVATLPDEA WERARRRAEI ISPLAQSETV GHEAADMAAQ ALGLSRRQVY VLIRRARQGS GLVTDLVPGQ SGGGKGKGRL PEPVERVIHE LLQKRFLTKQ KRSLAAFHRE VTQVCKAQKL RVPARNTVAL RIASLDPRKV IRRREGQDAA RDLQGVGGEP PAVTAPLEQV QIDHTVIDLI VVDDRDRQPI GRPYLTLAID VFTRCVLGMV VTLEAPSAVS VGLCLVHVAC DKRPWLEGLN VEMDWQMSGK PLLLYLDNAA EFKSEALRRG CEQHGIRLDY RPLGQPHYGG IVERIIGTAM QMIHDELPGT TFSNPDQRGD YDSENKAALT LRELERWLTL AVGTYHGSVH NGLLQPPAAR WAEAVARVGV PAVVTRATSF LVDFLPILRR TLTRTGFVID HIHYYADALK PWIARRERWP SFLIRRDPRD ISRIWVLEPE GQHYLEIPYR TLSHPAVTLW EQRQALAKLR QQGREQVDES ALFRMIGQMR EIVTSAQKAT RKARRDADRR QHLKTSARPD KPVPPDTDIA DPQADNLPPA KPFDQIEEW |
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| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tniB | TniB | Tn402.10 | 302 | 1824-2732 | + |
|---|
| Class: | Accessory Gene | ||||||||||
| Sequence Family: | ATP binding protein? | ||||||||||
| Comment: | identical to tniB (Tn1721)| similar function to Tn7 tnsC and MuB | ||||||||||
| Protein Sequence: | MDEYPIIDLS HLLPAAQGLA RLPADERIQR LRADRWIGYP RAVEALNRLE ALYAWPNKQR MPNLLLVGPT NNGKSMIVEK FRRTHPASSD ADQEHIPVLV VQMPSEPSVI RFYVALLAAM GAPLRPRPRL PEMEQLALAL LRKVGVRMLV IDELHNVLAG NSVNRREFLN LLRFLGNELR IPLVGVGTRD AYLAIRSDDQ LENRFEPMML PVWEANDDCC SLLASFAASL PLRRPSPIAT LDMARYLLTR SEGTIGELAH LLMAAAIVAV ESGEEAINHR TLSMAVYTGP SERRRQFERE LM |
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| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tniQ | TniQ | Tn402.10 | 405 | 2729-3946 | + |
|---|
| Class: | Accessory Gene | ||||||||||
| Subclass: | Target Site Selection | ||||||||||
| Comment: | identical to tniQ (Tn1721)|similar function to Tn7 tnsD? | ||||||||||
| Protein Sequence: | MKPAPRWPLH PAPKEGEALS SWLNRVALCY HMEEPDLLEH DLGHGQVDDL DTAPPLSLLA LLSQRSGIEL DRLRCMSFAG WVPWLLDSLD DQIPDALETY AFQLSVLLPR LRRKTRSITS WRAWLPSQPI NRACPLCLSD PENQAVLLAW KLPLMLSCPL HGCWLESYWG VPGRFLGWEN ADAEPRTASD AIAAMDQRTW QALTTGHVEL PRRRIHAGLW FRLLRTLLDE LNTPLSACGT CAGYPRQVWE GCGHPLRAGQ SLWRPYETLN PIVRLQMLEA AATAISLIEV RDISPPGEQA KLFWSEPQTG FTSGLPTKAP KPEPINHWQR AVQAIDEAII EARHNPETAR SLFALASYGR RDPASLERLR ATFVKEGIPP EFLSHYLPDA PFACLKQNDG LSDKF |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tniR | TniR | Tn402.10 | 207 | 4008-4631 | + |
|---|
| Class: | Accessory Gene | ||||||||||
| Subclass: | Resolvase | ||||||||||
| Sequence Family: | Serine Site-Specific Recombinase | ||||||||||
| Transposase Chemistry: | Serine | ||||||||||
| Comment: | resolution of cointegrates || Protein: ACE81792.1 || identical to tniR(Tn1721) | ||||||||||
| Protein Sequence: | MLIGYMRVSK ADGSQSTNLQ RDALIAAGVS LAHLYEDLAS GRRDDRPGLA ACLKALREGD TLIVWKLDRL GRDLRHLINT VHDLTARSVG LKVLTGHGAA VDTTTAAGKL VFGIFAALAE FERELISERT VAGLISARAR GRKGGRPFKM TAAKLRLAMA SMGQPETKVG DLCEELGITR QTLYRHVSPK GELRPDGVKL LSLGSAA |
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| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | qacE (ARO:3005009) | QacE | Tn402.10 | 110 | 4880-5212 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antibiotic Resistance | ||||||||||
| Function: | antibiotic efflux (ARO:0010000) | ||||||||||
| Sequence Family: | major facilitator superfamily (MFS) antibiotic efflux pump (ARO:0010002) | ||||||||||
| Target: | quaternary ammonium salts | ||||||||||
| Comment: | SMR export pump||strict match to reference sequence for ARO:3005009 (bitscore:203) | ||||||||||
| Protein Sequence: | MKGWLFLVIS IVGEVIATSA LKSSEGFTKL APSAVVIIGY GIAFYFLSLV LKSIPVGVAY AVWSGLGVVI ITAIAWLLHG QKLDAWGFVG MGLIVSGVVV LNLLSKASAH |
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| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | AAC(6')-Ib4 (ARO:3002577) | AAC(6')-Ib4 | Tn402.10 | 184 | 5381-5935 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antibiotic Resistance | ||||||||||
| Function: | antibiotic inactivation (ARO:0001004) | ||||||||||
| Sequence Family: | AAC(6') (ARO:3000345) | ||||||||||
| Target: | aminoglycoside antibiotic (ARO:0000016) | ||||||||||
| Comment: | strict match to reference sequence for ARO:3002577 (bitscore: 380) | ||||||||||
| Protein Sequence: | MTNSNDSVTL RLMTEHDLAM LYEWLNRSHI VEWWGGEEAR PTLADVQEQY LPSVLAQESV TPYIAMLNGE PIGYAQSYVA LGSGDGWWEE ETDPGVRGID QSLANASQLG KGLGTKLVRA LVELLFNDPE VTKIQTDPSP SNLRAIRCYE KAGFERQGTV TTPDGPAVYM VQTRQAFERT RSVA |
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| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | bla VIM-1 (ARO:3002271) | Bla VIM-1 | Tn402.10 | 266 | 6043-6843 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antibiotic Resistance | ||||||||||
| Function: | antibiotic inactivation (ARO:0001004) | ||||||||||
| Sequence Family: | VIM beta-lactamase (ARO:3000021) | ||||||||||
| Target: | carbapenem (ARO:0000020)||cephamycin (ARO:0000044)||penam (ARO:3000008)||penem (ARO:3003706)||cephalosporin (ARO:0000032) | ||||||||||
| Comment: | strict match to reference sequence for ARO:3002271 (bitscore: 536) | ||||||||||
| Protein Sequence: | MLKVISSLLV YMTASVMAVA SPLAHSGEPS GEYPTVNEIP VGEVRLYQIA DGVWSHIATQ SFDGAVYPSN GLIVRDGDEL LLIDTAWGAK NTAALLAEIE KQIGLPVTRA VSTHFHDDRV GGVDVLRAAG VATYASPSTR RLAEAEGNEI PTHSLEGLSS SGDAVRFGPV ELFYPGAAHS TDNLVVYVPS ANVLYGGCAV HELSSTSAGN VADADLAEWP TSVERIQKHY PEAEVVIPGH GLPGGLDLLQ HTANVVKAHK NRSVAE |
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| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | intI1 | IntI1 | Tn402.10 | 337 | 7010-8023 | + |
|---|
| Class: | Integron Integrase | ||||||||||
| Subclass: | Class 1 | ||||||||||
| Sequence Family: | Class 1 Integron Tyrosine Integrase | ||||||||||
| Transposase Chemistry: | Tyrosine | ||||||||||
| Protein Sequence: | MKTATAPLPP LRSVKVLDQL RERIRYLHYS LRTEQAYVHW VRAFIRFHGV RHPATLGSSE VEAFLSWLAN ERKVSVSTHR QALAALLFFY GKVLCTDLPW LQEIGRPRPS RRLPVVLTPD EVVRILGFLE GEHRLFAQLL YGTGMRISEG LQLRVKDLDF DHGTIIVREG KGSKDRALML PESLAPSLRE QLSRARAWWL KDQAEGRSGV ALPDALERKY PRAGHSWPWF WVFAQHTHST DPRSGVVRRH HMYDQTFQRA FKRAVEQAGI TKPATPHTLR HSFATALLRS GYDIRTVQDL LGHSDVSTTM IYTHVLKVGG AGVRSPLDAL PPLTSER |
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