Mobile element type:
Transposon
Name:
Tn4001
Synonyms:
Tn4003| Tn3706| Tn5281
Accession:
Tn4001-GU235985
Family:
Compound Transposon
Group:
IS256
First isolate:
Yes
Partial:
ND
Evidence of transposition:
Yes
Host Organism:
Staphylococcus aureus strain SK529
Date of Isolation:
1984
Country:
Australia
Molecular Source:
plasmid pSK1
ORF Summary
| Name | Associated TE | Coordinates | Orientation | Class | Subclass |
|---|---|---|---|---|---|
| tnp_truncated | IS256.var1 | 102-968 | + | Transposase | |
| GNAT_fam | Tn4001 | 1346-1711 | + | Passenger Gene | Antibiotic Resistance |
| AAC(6')-Ie-APH(2'')-Ia (ARO:3002597) | Tn4001 | 1712-3151 | + | Passenger Gene | Antibiotic Resistance |
| tnp | IS256 | 3281-4453 | - | Transposase |
ORF Details
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnp_truncated | Tnp_truncated | IS256.var1 | 288 | 102-968 | + |
|---|
| Class: | Transposase | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Comment: | 1bp deletion relative to tnp (IS256) causes frameshift and premature termination | ||||||||||
| Protein Sequence: | MTQVHFTLKS EEIQSIIEYS VKDDVSKNIL TTVFNQLMEN QRTEYIQAKE YERTENRQSQ RNGYYERSFT TRVGTLELKV PRTRDGHFSP TVFERYQRNE KALMASMLEM YVSGVSTRKV SKIVEELCGK SVSKSFVSSL TEQLEPMVNE WQNRLLSEKN YPYLMTDVLY IKVREENRVL SKSCHIAIGI TKDGDREIIG FMIQSGESEE TWTTFFEYLK ERGLQGTELV ISDAHKGLVS AIRKSFTNVS WQRCQVHFLR NIFTTIPKKI QNLSEKLLKE FLSSQILT |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | GNAT_fam | GNAT_fam | Tn4001 | 121 | 1346-1711 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antibiotic Resistance | ||||||||||
| Sequence Family: | Acetyltransf_1 (Pfam:PF00583) | ||||||||||
| Target: | aminoglycoside antibiotic (ARO:0000016) | ||||||||||
| Comment: | putative acetyltransferase | ||||||||||
| Protein Sequence: | MVFDIDNLKG FLNDTSSFGF IAKENNKIIG FAYCYTLLRP DGKTMFYLHS IGMLPNYQDK GYGSKLLSFI KEYSKEIGCS EMFLITDKGN PRACHVYEKL GGKNDYKDEI VYVYDYEKGD K |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | AAC(6')-Ie-APH(2'')-Ia (ARO:3002597) | AAC(6')-Ie-APH(2'')-Ia | Tn4001 | 479 | 1712-3151 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antibiotic Resistance | ||||||||||
| Function: | antibiotic inactivation (ARO:0001004) | ||||||||||
| Sequence Family: | APH(2'')(ARO:3000128)||AAC(6') (ARO:3000345) | ||||||||||
| Target: | aminoglycoside antibiotic (ARO:0000016) | ||||||||||
| Comment: | strict match to reference sequence for ARO:3002597 (bitscore: 966)||Synonyms: aac(6')-bifunctionalaacA-aphD APH(2''), aph(2'')-bifunctional, AAC(6')-APH(2'') | ||||||||||
| Protein Sequence: | MNIVENEICI RTLIDDDFPL MLKWLTDERV LEFYDGRDKK YTLESLKKHY TEPWEDEVFR VIIEYNNVPI GYGQIYKMYD ELYTDYHYPK TDEIVYGMDQ FIGEPNYWSK GIGTRYIKLI FEFLKKERNA NAVILDPHKN NPRAIRAYQK SGFRIIEDLP EHELHEGKKE DCYLMEYRYD DNATNVKAMK YLIEHYFDNF KVDSIEIIGS GYDSVAYLVN NEYIFKTKFS TNKKKGYAKE KAIYNFLNTN LETNVKIPNI EYSYISDELS ILGYKEIKGT FLTPEIYSTM SEEEQNLLKR DIASFLRQMH GLDYTDISEC TIDNKQNVLE EYILLRETIY NDLTDIEKDY IESFMERLNA TTVFEGKKCL CHNDFSCNHL LLDGNNRLTG IIDFGDSGII DEYCDFIYLL EDSEEEIGTN FGEDILRMYG NIDIEKAKEY QDIVEEYYPI ETIVYGIKNI KQEFIENGRK EIYKRTYKD |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnp | Tnp | IS256 | 390 | 3281-4453 | - |
|---|
| Class: | Transposase | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Protein Sequence: | MTQVHFTLKS EEIQSIIEYS VKDDVSKNIL TTVFNQLMEN QRTEYIQAKE YERTENRQSQ RNGYYERSFT TRVGTLELKV PRTRDGHFSP TVFERYQRNE KALMASMLEM YVSGVSTRKV SKIVEELCGK SVSKSFVSSL TEQLEPMVNE WQNRLLSEKN YPYLMTDVLY IKVREENRVL SKSCHIAIGI TKDGDREIIG FMIQSGESEE TWTTFFEYLK ERGLQGTELV ISDAHKGLVS AIRKSFTNVS WQRCQVHFLR NIFTTIPKKN SKSFREAVKG IFKFTDINLA REAKNRLIHD YIDQPKYSKA CASLDDGFED AFQYTVQGNS HNRLKSTNLI ERLNQEVRRR EKIIRIFPNQ TSANRLIGAV LMDLHDEWIY SSRKYINFDK |
||||||||||
| TnCentral Accession | TE Name | Type | Coordinates | Strand | Length |
|---|---|---|---|---|---|
| IS256.var1-GU235985 | IS256.var1 | insertion sequence | 1-1323 | + | 1323 |
| IS256-M18086 | IS256 | insertion sequence | 3231-4554 | + | 1324 |
| Name | Associated TE | Coordinates | Direction | Length | Sequence |
|---|---|---|---|---|---|
| IRL | IS256.var1 | 1-26 | + | 26 | GATAAAGTCC GTATAATTGT GTAAAA |
| IRR | IS256.var1 | 1298-1323 | - | 26 | AGTCAAGTCC AGACTCCTGT GTAAAA |
| IRR | IS256 | 3231-3256 | + | 26 | AGTCAAGTCC AGACTCCTGT GTAAAA |
| IRL | IS256 | 4529-4554 | - | 26 | GATAAAGTCC GTATAATTGT GTAAAA |
1. Lyon BR, May JW, Skurray RA.
Tn4001: a gentamicin and kanamycin resistance transposon in Staphylococcus aureus.. Mol Gen Genet. 1984;193(3):554-6. doi: 10.1007/BF00382099. PubMed ID:
6323927
.
2. Lyon BR, Gillespie MT, Byrne ME, May JW, Skurray RA. Plasmid-mediated resistance to gentamicin in Staphylococcus aureus: the involvement of a transposon.. J Med Microbiol. 1987 Mar;23(2):101-10. doi: 10.1099/00222615-23-2-101. PubMed ID: 3031300 .
3. Gillespie MT, Lyon BR, Messerotti LJ, Skurray RA. Chromosome- and plasmid-mediated gentamicin resistance in Staphylococcus aureus encoded by Tn4001.. J Med Microbiol. 1987 Sep;24(2):139-44. doi: 10.1099/00222615-24-2-139. PubMed ID: 2821261 .
4. Rouch DA, Byrne ME, Kong YC, Skurray RA. The aacA-aphD gentamicin and kanamycin resistance determinant of Tn4001 from Staphylococcus aureus: expression and nucleotide sequence analysis.. J Gen Microbiol. 1987 Nov;133(11):3039-52. doi: 10.1099/00221287-133-11-3039. PubMed ID: 2833561 .
5. Mahairas GG, Minion FC. Random insertion of the gentamicin resistance transposon Tn4001 in Mycoplasma pulmonis.. Plasmid. 1989 Jan;21(1):43-7. doi: 10.1016/0147-619x(89)90085-1. PubMed ID: 2543007 .
6. Mahairas GG, Lyon BR, Skurray RA, Pattee PA. Genetic analysis of Staphylococcus aureus with Tn4001.. J Bacteriol. 1989 Jul;171(7):3968-72. doi: 10.1128/jb.171.7.3968-3972.1989. PubMed ID: 2544565 .
7. Prudhomme M, Turlan C, Claverys JP, Chandler M. Diversity of Tn4001 transposition products: the flanking IS256 elements can form tandem dimers and IS circles.. J Bacteriol. 2002 Jan;184(2):433-43. doi: 10.1128/JB.184.2.433-443.2002. PubMed ID: 11751820 .
8. Hodel-Christian SL, Murray BE. Characterization of the gentamicin resistance transposon Tn5281 from Enterococcus faecalis and comparison to staphylococcal transposons Tn4001 and Tn4031.. Antimicrob Agents Chemother. 1991 Jun;35(6):1147-52. doi: 10.1128/AAC.35.6.1147. PubMed ID: 1656854 .
9. Horaud T, de Cespedes G, Trieu-Cuot P. Chromosomal gentamicin resistance transposon Tn3706 in Streptococcus agalactiae B128.. Antimicrob Agents Chemother. 1996 May;40(5):1085-90. doi: 10.1128/AAC.40.5.1085. PubMed ID: 8723445 .
10. Chen L, Mediavilla JR, Smyth DS, Chavda KD, Ionescu R, Roberts RB, Robinson DA, Kreiswirth BN. Identification of a novel transposon (Tn6072) and a truncated staphylococcal cassette chromosome mec element in methicillin-resistant Staphylococcus aureus ST239.. Antimicrob Agents Chemother. 2010 Aug;54(8):3347-54. doi: 10.1128/AAC.00001-10. Epub 2010 May 17. PubMed ID: 20479198 .
2. Lyon BR, Gillespie MT, Byrne ME, May JW, Skurray RA. Plasmid-mediated resistance to gentamicin in Staphylococcus aureus: the involvement of a transposon.. J Med Microbiol. 1987 Mar;23(2):101-10. doi: 10.1099/00222615-23-2-101. PubMed ID: 3031300 .
3. Gillespie MT, Lyon BR, Messerotti LJ, Skurray RA. Chromosome- and plasmid-mediated gentamicin resistance in Staphylococcus aureus encoded by Tn4001.. J Med Microbiol. 1987 Sep;24(2):139-44. doi: 10.1099/00222615-24-2-139. PubMed ID: 2821261 .
4. Rouch DA, Byrne ME, Kong YC, Skurray RA. The aacA-aphD gentamicin and kanamycin resistance determinant of Tn4001 from Staphylococcus aureus: expression and nucleotide sequence analysis.. J Gen Microbiol. 1987 Nov;133(11):3039-52. doi: 10.1099/00221287-133-11-3039. PubMed ID: 2833561 .
5. Mahairas GG, Minion FC. Random insertion of the gentamicin resistance transposon Tn4001 in Mycoplasma pulmonis.. Plasmid. 1989 Jan;21(1):43-7. doi: 10.1016/0147-619x(89)90085-1. PubMed ID: 2543007 .
6. Mahairas GG, Lyon BR, Skurray RA, Pattee PA. Genetic analysis of Staphylococcus aureus with Tn4001.. J Bacteriol. 1989 Jul;171(7):3968-72. doi: 10.1128/jb.171.7.3968-3972.1989. PubMed ID: 2544565 .
7. Prudhomme M, Turlan C, Claverys JP, Chandler M. Diversity of Tn4001 transposition products: the flanking IS256 elements can form tandem dimers and IS circles.. J Bacteriol. 2002 Jan;184(2):433-43. doi: 10.1128/JB.184.2.433-443.2002. PubMed ID: 11751820 .
8. Hodel-Christian SL, Murray BE. Characterization of the gentamicin resistance transposon Tn5281 from Enterococcus faecalis and comparison to staphylococcal transposons Tn4001 and Tn4031.. Antimicrob Agents Chemother. 1991 Jun;35(6):1147-52. doi: 10.1128/AAC.35.6.1147. PubMed ID: 1656854 .
9. Horaud T, de Cespedes G, Trieu-Cuot P. Chromosomal gentamicin resistance transposon Tn3706 in Streptococcus agalactiae B128.. Antimicrob Agents Chemother. 1996 May;40(5):1085-90. doi: 10.1128/AAC.40.5.1085. PubMed ID: 8723445 .
10. Chen L, Mediavilla JR, Smyth DS, Chavda KD, Ionescu R, Roberts RB, Robinson DA, Kreiswirth BN. Identification of a novel transposon (Tn6072) and a truncated staphylococcal cassette chromosome mec element in methicillin-resistant Staphylococcus aureus ST239.. Antimicrob Agents Chemother. 2010 Aug;54(8):3347-54. doi: 10.1128/AAC.00001-10. Epub 2010 May 17. PubMed ID: 20479198 .