Mobile element type:
Transposon
Name:
Tn3
Synonyms:
Accession:
Tn3-V00613
Family:
Tn3
Group:
Tn3
First isolate:
Yes
Partial:
ND
Evidence of transposition:
Yes
Host Organism:
S. paratyphi B
Date of Isolation:
1967
Country:
England
Molecular Source:
plasmid R1
| Name | Coordinates | Direction | Length | Sequence |
|---|---|---|---|---|
| IRL | 1-38 | + | 38 | GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAG |
| internal IR | 1103-1140 | + | 38 | GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGA |
| IRR | 4920-4957 | - | 38 | GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAG |
| Name | Associated TE | Coordinates | Orientation | Length | Sequence |
|---|---|---|---|---|---|
| res | Tn3 | 1751-1871 | + | 121 | AAATGTACCT TAAATCGAAT ATCAGACACG ATGTGTCTAT TATGCCAAAA TGACGATTTA ATGGACACTC AAACGAAGCC GTTTTACTAT GTCTGATAAT TTATAATATT TCGAACGGTT G |
| res_site_III_a | Tn3 | 1754-1776 | + | 23 | TGTACCTTAA ATCGAATATC AGA |
| res_site_II_a | Tn3 | 1782-1817 | + | 36 | TGTGTCTATT ATGCCAAAAT GACGATTTAA TGGACA |
| res_site_I | Tn3 | 1840-1868 | + | 29 | TGTCTGATAA TTTATAATAT TTCGAACGG |
ORF Summary
| Name | Associated TE | Coordinates | Orientation | Class | Subclass |
|---|---|---|---|---|---|
| bla TEM-1 (ARO:3000873) | Tn3 | 148-1008 | - | Passenger Gene | Antibiotic Resistance |
| tnpR | Tn3 | 1191-1748 | - | Accessory Gene | Resolvase |
| tnpA | Tn3 | 1910-4924 | + | Transposase |
ORF Details
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | bla TEM-1 (ARO:3000873) | Bla TEM-1 | Tn3 | 286 | 148-1008 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antibiotic Resistance | ||||||||||
| Function: | antibiotic inactivation (ARO:0001004) | ||||||||||
| Sequence Family: | TEM beta-lactamase (ARO:3000014) | ||||||||||
| Target: | penem (ARO:3003706)||cephalosporin (ARO:0000032)||monobactam (ARO:0000004)||penam (ARO:3000008) | ||||||||||
| Comment: | perfect match to reference sequence for ARO:3000873||Synonyms: TEM-98, RTEM-1 | ||||||||||
| Protein Sequence: | MSIQHFRVAL IPFFAAFCLP VFAHPETLVK VKDAEDQLGA RVGYIELDLN SGKILESFRP EERFPMMSTF KVLLCGAVLS RVDAGQEQLG RRIHYSQNDL VEYSPVTEKH LTDGMTVREL CSAAITMSDN TAANLLLTTI GGPKELTAFL HNMGDHVTRL DRWEPELNEA IPNDERDTTM PAAMATTLRK LLTGELLTLA SRQQLIDWME ADKVAGPLLR SALPAGWFIA DKSGAGERGS RGIIAALGPD GKPSRIVVIY TTGSQATMDE RNRQIAEIGA SLIKHW |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpR | TnpR | Tn3 | 185 | 1191-1748 | - |
|---|
| Class: | Accessory Gene | ||||||||||
| Subclass: | Resolvase | ||||||||||
| Function: | resolvase||serine site-specific recombinase | ||||||||||
| Sequence Family: | Serine Site-Specific Recombinase | ||||||||||
| Transposase Chemistry: | Serine | ||||||||||
| Comment: | first defined as a repressor | ||||||||||
| Protein Sequence: | MRIFGYARVS TSQQSLDIQI RALKDAGVKA NRIFTDKASG SSTDREGLDL LRMKVEEGDV ILVKKLDRLG RDTADMIQLI KEFDAQGVAV RFIDDGISTD GDMGQMVVTI LSAVAQAERR RILERTNEGR QEAKLKGIKF GRRRTVDRNV VLTLHQKGTG ATEIAHQLSI ARSTVYKILE DERAS |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpA | TnpA | Tn3 | 1004 | 1910-4924 | + |
|---|
| Class: | Transposase | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Protein Sequence: | MPVDFLTTEQ VESYGRFTGE PDELQLARYF HLDEADKEFI GKSRGDHNRL GIALQIGCVR FLGTFLTDMN HIPSGVRHFT ARQLGIRDIT VLAEYGQREN TRREHAALIR QHYQYREFAW PWTFRLTRLL YTRSWISNER PGLLFDLATG WLMQHRIILP GATTLTRLIS EVREKATLRL WNKLALIPSA EQRSQLEMLL GPTDCSRLSL LESLKKGPVT ISGPAFNEAI ERWKTLNDFG LHAENLSTLP AVRLKNLARY AGMTSVFNIA RMSPQKRMAV LVAFVLAWET LALDDALDVL DAMLAVIIRD ARKIGQKKRL RSLKDLDKSA LALASACSYL LKEETPDESI RAEVFSYIPR QKLAEIITLV REIARPSDDN FHDEMVEQYG RVRRFLPHLL NTVKFSSAPA GVTTLNACDY LSREFSSRRQ FFDDAPTEII SQSWKRLVIN KEKHITRRGY TLCFLSKLQD SLRRRDVYVT GSNRWGDPRA RLLQGADWQA NRIKVYRSLG HPTDPQEAIK SLGHQLDSRY RQVAARLGEN EAVELDVSGP KPRLTISPLA SLDEPDSLKR LSKMISDLLP PVDLTELLLE INAHTGFADE FFHASEASAR VDDLPVSISA VLMAEACNIG LEPLIRSNVP ALTRHRLNWT KANYLRAETI TSANARLVDF QATLPLAQIW GGGEVASADG MRFVTPVRTI NAGPNRKYFG NNRGITWYNF VSDQYSGFHG FHGIVIPGTL RDSIFVLEGL LEQETGLNPT EIMTDTAGAS DLVFGLFWLL GYQFSPRLAD AGASVFWRMD HDADYGVLND IARGQSDPRK IVLQWDEMIR TAGSLKLGKV QASVLVRSLL KSERPSGLTQ AIIEVGRINK TLYLLNYIDD EDYRRRILTQ LNRGESRHAV ARAICHGQKG EIRKRYTDGQ EDQLGALGLV TNAVVLWNTI YMQAALDHLR AQGETLNDED IARLSPLCHG HINMLGHYSF TLAELVTKGH LRPLKEASEA ENVA |
||||||||||
1. Kretschmer PJ, Cohen SN.
Selected translocation of plasmid genes: frequency and regional specificity of translocation of the Tn3 element.. J Bacteriol. 1977 May;130(2):888-99. doi: 10.1128/jb.130.2.888-899.1977. PubMed ID:
233726
.
2. Crisona NJ, Clark AJ. Increase in conjugational transmission frequency of nonconjugative plasmids.. Science. 1977 Apr 8;196(4286):186-7. doi: 10.1126/science.322280. PubMed ID: 322280 .
3. Goebel W, Lindenmaier W, Pfeifer F, Schrempf H, Schelle B. Transposition and insertion of intact, deleted and enlarged ampicillin transposon Tn3 from mini-R1 (Rsc) plasmids into transfer factors.. Mol Gen Genet. 1977 Nov 29;157(2):119-29. doi: 10.1007/BF00267389. PubMed ID: 340918 .
4. Nisen PD, Kopecko DJ, Chou J, Cohen SN. Site-specific DNA deletions occurring adjacent to the termini of a transposable ampicillin resistance element (Tn3).. J Mol Biol. 1977 Dec 25;117(4):975-8. doi: 10.1016/s0022-2836(77)80008-9. PubMed ID: 606841 .
5. Sutcliffe JG. Nucleotide sequence of the ampicillin resistance gene of Escherichia coli plasmid pBR322.. Proc Natl Acad Sci U S A. 1978 Aug;75(8):3737-41. doi: 10.1073/pnas.75.8.3737. PubMed ID: 358200 .
6. Heffron F, McCarthy BJ, Ohtsubo H, Ohtsubo E. DNA sequence analysis of the transposon Tn3: three genes and three sites involved in transposition of Tn3.. Cell. 1979 Dec;18(4):1153-63. doi: 10.1016/0092-8674(79)90228-9. PubMed ID: 391406 .
7. Wishart WL, Machida C, Ohtsubo H, Ohtsubo E. Escherichia coli RNA polymerase binding sites and transcription initiation sites in the transposon Tn3.. Gene. 1983 Sep;24(1):99-113. doi: 10.1016/0378-1119(83)90135-x. PubMed ID: 6313485 .
2. Crisona NJ, Clark AJ. Increase in conjugational transmission frequency of nonconjugative plasmids.. Science. 1977 Apr 8;196(4286):186-7. doi: 10.1126/science.322280. PubMed ID: 322280 .
3. Goebel W, Lindenmaier W, Pfeifer F, Schrempf H, Schelle B. Transposition and insertion of intact, deleted and enlarged ampicillin transposon Tn3 from mini-R1 (Rsc) plasmids into transfer factors.. Mol Gen Genet. 1977 Nov 29;157(2):119-29. doi: 10.1007/BF00267389. PubMed ID: 340918 .
4. Nisen PD, Kopecko DJ, Chou J, Cohen SN. Site-specific DNA deletions occurring adjacent to the termini of a transposable ampicillin resistance element (Tn3).. J Mol Biol. 1977 Dec 25;117(4):975-8. doi: 10.1016/s0022-2836(77)80008-9. PubMed ID: 606841 .
5. Sutcliffe JG. Nucleotide sequence of the ampicillin resistance gene of Escherichia coli plasmid pBR322.. Proc Natl Acad Sci U S A. 1978 Aug;75(8):3737-41. doi: 10.1073/pnas.75.8.3737. PubMed ID: 358200 .
6. Heffron F, McCarthy BJ, Ohtsubo H, Ohtsubo E. DNA sequence analysis of the transposon Tn3: three genes and three sites involved in transposition of Tn3.. Cell. 1979 Dec;18(4):1153-63. doi: 10.1016/0092-8674(79)90228-9. PubMed ID: 391406 .
7. Wishart WL, Machida C, Ohtsubo H, Ohtsubo E. Escherichia coli RNA polymerase binding sites and transcription initiation sites in the transposon Tn3.. Gene. 1983 Sep;24(1):99-113. doi: 10.1016/0378-1119(83)90135-x. PubMed ID: 6313485 .