Mobile element type:
Transposon
Name:
Tn2424
Synonyms:
Accession:
Tn2424-UGCJ01000005
Family:
Tn3
Group:
Tn21
First isolate:
Yes
Partial:
ND
Evidence of transposition:
Yes
Host Organism:
Escherichia coli NCTC11186
Date of Isolation:
2018
Country:
United kingdom
| Name | Coordinates | Direction | Length | Sequence |
|---|---|---|---|---|
| IRL | 1-38 | + | 38 | GGGGGCACCT CAGAAAACGG AAAATAAAGC ACGCTAAG |
| repeat i4 | 10-28 | + | 19 | TCAGAAAACG GAAAATAAA |
| IRR | 25968-26008 | - | 41 | GGGGTCGTCT CAGAAAACGG AAAATAAAGC ACGCTAAGCC G |
| repeat i4 | 25981-25999 | - | 19 | TCAGAAAACG GAAAATAAA |
| Name | Associated TE | Coordinates | Orientation | Length | Sequence |
|---|---|---|---|---|---|
| terIS | ISEc37.1 | 11587-11686 | + | 100 | TGAACGGGCC ATATAACCAC CTGAAAGACA ATGACATTGC CTGTTTTTAT AACGGTAATT GCAGACCATG ACAAGCCGCA GCCGTCAGGC TGCCTACTCG |
| attC qacEdelta1_sul1 | In21 | 13935-13968 , 15246-15252 | + | 41 | CCGCTAGCGG GCGGCCGGAA GGTGAATGCT AGGCATCTAA C |
| attC qacEdelta1_sul1 core | In21 | 13935-13968 | + | 34 | CCGCTAGCGG GCGGCCGGAA GGTGAATGCT AGGC |
| attC catB2 | In21 | 15252-15317 , 15985-15990 | + | 72 | CGCCACGCTC ACCGGCAAAT TAGGAGCGCA GCGAGTAATT TGTCCGTGTG TAGCGTATTG TTAGGCGCCT AA |
| attC catB2 core | In21 | 15252-15317 | + | 66 | CGCCACGCTC ACCGGCAAAT TAGGAGCGCA GCGAGTAATT TGTCCGTGTG TAGCGTATTG TTAGGC |
| attC orfM | In21 | 15991-16098 , 16448-16453 | + | 114 | CATGTATTAG ACCGCCTTCT ACATAAGCCA ATCATCGTGA AGGCGGTATA ACCCGATGCG ACTAAATTTT GATTGCCCGA TTAGACCATA GGGATCAACC CGTTACAAGT TTAA |
| attC orfM core | In21 | 15991-16098 | + | 108 | CATGTATTAG ACCGCCTTCT ACATAAGCCA ATCATCGTGA AGGCGGTATA ACCCGATGCG ACTAAATTTT GATTGCCCGA TTAGACCATA GGGATCAACC CGTTACAA |
| attC parE_phd | In21 | 16454-16541 , 17169-17174 | + | 94 | CTTGTTGCCG CCAAAACGGC TGGTGCCCGG GGTGGTTCAT GAAACACCCT CTGTTTCTGC AGGAGCGGGG GCTATTGACT GGTTATTCGC ATAA |
| attC parE_phd core | In21 | 16454-16541 | + | 88 | CTTGTTGCCG CCAAAACGGC TGGTGCCCGG GGTGGTTCAT GAAACACCCT CTGTTTCTGC AGGAGCGGGG GCTATTGACT GGTTATTC |
| attC orfJ core | In21 | 17175-17242 | + | 68 | CGCTAAGCTC AGCCGCAGTT TATAAGTCGA GCGCACAAAG TGCGCATTGC TGGAGCGCCT TGTTAACT |
| attC orfI | In21 | 17551-17621 , 18143-18148 | + | 77 | CGTAGAGCTA AGAGGCGCTG CTTGCGGCGT CCACGGTAGC CGAAGGCTGC GCCTTGAGCG CCTGGTTAGG TACCTAA |
| attC orfI core | In21 | 17551-17621 | + | 71 | CGTAGAGCTA AGAGGCGCTG CTTGCGGCGT CCACGGTAGC CGAAGGCTGC GCCTTGAGCG CCTGGTTAGG T |
| attC orfH | In21 | 18149-18228 , 18595-18600 | + | 86 | CGTAGAGCTA ACCGGCGCTG CGCGGCCTTA TCGCGCAGCG TCCAGCAACT GAAGGGAGCG AGGTTGAGGC CAGGTTAGGC GCCTAA |
| attC orfH core | In21 | 18149-18228 | + | 80 | CGTAGAGCTA ACCGGCGCTG CGCGGCCTTA TCGCGCAGCG TCCAGCAACT GAAGGGAGCG AGGTTGAGGC CAGGTTAGGC |
| attC AAC(6')-Ia_orfG | In21 | 18601-18712 , 19753-19758 | + | 118 | CATTTGCTTA ACCTGCAATT CCGGCCCGAA GGGCTTGGCG TGGTTTGTGC TGAGCAAAGC GACAGCACAA ACCATGACAA CAGGAATTGT CAGGTTGAAG CATTTGTTAG GGCCCTAA |
| attC AAC(6')-Ia core | In21 | 18601-18712 | + | 112 | CATTTGCTTA ACCTGCAATT CCGGCCCGAA GGGCTTGGCG TGGTTTGTGC TGAGCAAAGC GACAGCACAA ACCATGACAA CAGGAATTGT CAGGTTGAAG CATTTGTTAG GG |
| attC aadA3 | In21 | 19759-19812 , 20609-20614 | + | 60 | CGCTTGAGTT AAGCCGCGCC GCGAAGCGGC GTCGGCTTGA ACGAATTGTT AGACGTCTAA |
| attC aadA1a core | In21 | 19759-19812 | + | 54 | CGCTTGAGTT AAGCCGCGCC GCGAAGCGGC GTCGGCTTGA ACGAATTGTT AGAC |
| attI | In21 | 20615-20670 | + | 56 | CTTTGTTTTA GGGCGACTGC CCTGCTGCGT AACATCGTTG CTGCTCCATA ACATCA |
| res | Tn2424 | 22305-22435 | + | 131 | GCCGCCGTCA GGTTGAGGCA TACCCTAACC TGATGTCAGA TGCCATGTGT AAATTGCGTC AGGATAGGAT TGAATTTTGA ATTTATTGAC ATATCTCGTT GAAGGTCATA GAGTCTTCCC TGACATTTTG C |
| res_site_I | Tn2424 | 22305-22343 | + | 39 | GCCGCCGTCA GGTTGAGGCA TACCCTAACC TGATGTCAG |
| res_site_II | Tn2424 | 22357-22400 | + | 44 | ATTGCGTCAG GATAGGATTG AATTTTGAAT TTATTGACAT ATCT |
| res_site_III | Tn2424 | 22404-22435 | + | 32 | TGAAGGTCAT AGAGTCTTCC CTGACATTTT GC |
ORF Summary
| Name | Associated TE | Coordinates | Orientation | Class | Subclass |
|---|---|---|---|---|---|
| merR | Tn2424 | 34-468 | - | Passenger Gene | Heavy Metal Resistance |
| merT | Tn2424 | 540-890 | + | Passenger Gene | Heavy Metal Resistance |
| merP | Tn2424 | 904-1179 | + | Passenger Gene | Heavy Metal Resistance |
| merC | Tn2424 | 1215-1637 | + | Passenger Gene | Heavy Metal Resistance |
| merA | Tn2424 | 1689-3383 | + | Passenger Gene | Heavy Metal Resistance |
| merD | Tn2424 | 3401-3763 | + | Passenger Gene | Heavy Metal Resistance |
| merE | Tn2424 | 3760-3996 | + | Passenger Gene | Heavy Metal Resistance |
| urfM 5'-end | Tn2424 | 3993-4663 | + | Passenger Gene | Other |
| tniA | In21 | 4775-6454 | + | Transposase | |
| tniB delta1 | In21 | 6457-7317 | + | Accessory Gene | |
| tnp | IS1353 | 7368-8912 | - | Transposase | |
| istA N-ter | IS1326_IS1353_ISEc37.1 | 9044-9865 | + | Transposase | None |
| tnp | ISEc37.1 | 10046-11233 | - | Transposase | |
| orf2 | ISEc37.1 | 11233-11598 | - | Accessory Gene | |
| istA C-ter | IS1326_IS1353_ISEc37.1 | 11687-12387 | + | Transposase | None |
| istB | IS1326_IS1353_ISEc37.1 | 12374-13159 | + | Accessory Gene | ATPase Transposition Helper |
| GNAT_fam | In21 | 13335-13835 | - | Passenger Gene | Antibiotic Resistance |
| sul1 (ARO:3000410) | In21 | 13963-14802 | - | Passenger Gene | Antibiotic Resistance |
| qacEdelta1 (ARO:3005010) | In21 | 14796-15143 | - | Passenger Gene | Antibiotic Resistance |
| catB2 (ARO:3002675) | In21 | 15312-15944 | - | Passenger Gene | Antibiotic Resistance |
| orfM | In21 | 16093-16356 | - | Passenger Gene | Hypothetical |
| parE | In21 | 16536-16835 | - | Passenger Gene | Toxin |
| phd | In21 | 16836-17105 | - | Passenger Gene | Antitoxin |
| orfJ | In21 | 17269-17526 | - | Passenger Gene | Hypothetical |
| orfI | In21 | 17558-18136 | - | Passenger Gene | Hypothetical |
| orfH | In21 | 18257-18589 | - | Passenger Gene | Hypothetical |
| orfG | In21 | 18727-19152 | - | Passenger Gene | Hypothetical |
| AAC(6')-Ia (ARO:3002545) | In21 | 19157-19714 | - | Passenger Gene | Antibiotic Resistance |
| aadA3 (ARO:3002603) | In21 | 19814-20605 | - | Passenger Gene | Antibiotic Resistance |
| intI1 | In21 | 20754-21767 | + | Integron Integrase | Class 1 |
| tnpM | Tn2424 | 21970-22320 | + | Accessory Gene | Inhibitor |
| tnpR | Tn2424 | 22446-23006 | + | Accessory Gene | Resolvase |
| tnpA | Tn2424 | 23009-25975 | + | Transposase |
ORF Details
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merR | MerR | Tn2424 | 144 | 34-468 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Function: | activator-repressor of mer operon | ||||||||||
| Target: | Mercury | ||||||||||
| Protein Sequence: | MENNLENLTI GVFAKAAGVN VETIRFYQRK GLLREPDKPY GSIRRYGEAD VVRVKFVKSA QRLGFSLDEI AELLRLDDGT HCEEASSLAE HKLKDVREKM ADLARMETVL SELVCACHAR KGNVSCPLIA SLQGEAGLAR SAMP |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merT | MerT | Tn2424 | 116 | 540-890 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Function: | cytosolic mercuric ion transport protein | ||||||||||
| Target: | Mercury | ||||||||||
| Protein Sequence: | MSEPQNGRGA LFAGGLAAIL ASTCCLGPLV LVALGFSGAW IGNLTVLEPY RPLFIGAALV ALFFAWKRIY RPVQACKPGE VCAIPQVRAT YKLIFWIVAV LVLVALGFPY VVPFFY |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merP | MerP | Tn2424 | 91 | 904-1179 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Function: | mercury transport | ||||||||||
| Target: | Mercury | ||||||||||
| Protein Sequence: | MKKLFASLAL AAAVAPVWAA TQTVTLAVPG MTCAACPITV KKALSKVEGV SKVDVGFEKR EAVVTFDDTK ASVQKLTKAT ADAGYPSSVK Q |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merC | MerC | Tn2424 | 140 | 1215-1637 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Function: | transmembrane protein mercury transport | ||||||||||
| Target: | Mercury | ||||||||||
| Protein Sequence: | MGLMTRIADK TGALGSVVSA MGCAACFPAL ASFGAAIGLG FLSQYEGLFI SRLLPLFAAL AFLANALGWF SHRQWLRSLL GMIGPAIVFA ATVWLLGNWW TANLMYVGLA LMIGVSIWDF VSPAHRRCGP DGCELPAKRL |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merA | MerA | Tn2424 | 564 | 1689-3383 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Function: | mercuric ion reductase | ||||||||||
| Target: | Mercury | ||||||||||
| Protein Sequence: | MSTLKITGMT CDSCAVHVKD ALEKVPGVQS ADVSYAKGSA KLAIEVGTSP DALTAAVAGL GYRATLADAP SVSTPGGLLD KMRDLLGRND KTGSSGALHI AVIGSGGAAM AAALKAVEQG ARVTLIERGT IGGTCVNVGC VPSKIMIRAA HIAHLRRESP FDGGIAATTP TIQRTALLAQ QQARVDELRH AKYEGILEGN PAITVLHGSA RFKDNRNLIV QLNDGGERVV AFDRCLIATG ASPAVPPIPG LKDTPYWTST EALVSETIPK RLAVIGSSVV ALELAQAFAR LGAKVTILAR STLFFREDPA IGEAVTAAFR MEGIEVREHT QASQVAYING EGDGEFVLTT AHGELRADKL LVATGRAPNT RKLALDATGV TLTPQGAIVI DPGMRTSVEH IYAAGDCTDQ PQFVYVAAAA GTRAAINMTG GDAALNLTAM PAVVFTDPQV ATVGYSEAEA HHDGIKTDSR TLTLDNVPRA LANFDTRGFI KLVVEEGSGR LIGVQAVAPE AGELIQTAAL AIRNRMTVQE LADQLFPYLT MVEGLKLAAQ TFNKDVKQLS CCAG |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merD | MerD | Tn2424 | 120 | 3401-3763 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Function: | secondary regulatory protein | ||||||||||
| Target: | Mercury | ||||||||||
| Protein Sequence: | MSAYTVSQLA HNAGVSVHIV RDYLVRGLLR PVACTTGGYG VFDDAALQRL CFVRAAFEAG IGLDALARLC RALDAADGAQ AAAQLAVLRQ LVERRRAALA HLDAQLASMP AERAHEEALP |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merE | MerE | Tn2424 | 78 | 3760-3996 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Function: | mercury transport | ||||||||||
| Target: | Mercury | ||||||||||
| Comment: | similar to urf-1 in pKLH2 (GenBank AF213017), pKLH272 (Genbank Y08992), pMER610 (GenBank Y08993), pKLH210 (GenBank Y10102), Tn5036 (Genbank Y09025), orf1 in Tn501 (GenBank Z00027), and urf-1 in Tn5041 (GenBank X98999) | ||||||||||
| Protein Sequence: | MNAPDKLPPE TRQPVSGYLW GALAVLTCPC HLPILAAVLA GTTAGAFLGE HWGVAALALT GLFVLAVTRL LRAFRGGS |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | urfM 5'-end | N/A | Tn2424 | 223 | 3993-4663 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Other | ||||||||||
| Comment: | urfMORF interrupted by insertion of In2 | ||||||||||
| Protein Sequence: | MTSSQPAGWT AAELAQAAAR GQLDLHYQPL VDLRDHRIAG AEALMRWRHP RLGLLPPGQF LPLAESFGLM PEIGAWVLGE ACRQMHKWQG PAWQPFRLAI NVSASQVGPT FDDEVKRVLA DMALPAELLE IELTESVAFG NPALFASFDA LRAIGVRFAA DDFGTGYSCL QHLKCCPITT LKIDQSFVAR LPDDARDQTI VRAVIQLAHG LGMDVIFRRR LHQ |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tniA | TniA | In21 | 559 | 4775-6454 | + |
|---|
| Class: | Transposase | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Comment: | can be extended upstream by 12 amino acids| identical to tniA (Tn1721 and In2)| 25% amino acid sequence identity to TnsB from Tn7 | ||||||||||
| Protein Sequence: | MATDTPRIPE QGVATLPDEA WERARRRAEI ISPLAQSETV GHEAADMAAQ ALGLSRRQVY VLIRRARQGS GLVTDLVPGQ SGGGKGKGRL PEPVERVIHE LLQKRFLTKQ KRSLAAFHRE VTQVCKAQKL RVPARNTVAL RIASLDPRKV IRRREGQDAA RDLQGVGGEP PAVTAPLEQV QIDHTVIDLI VVDDRDRQPI GRPYLTLAID VFTRCVLGMV VTLEAPSAVS VGLCLVHVAC DKRPWLEGLN VEMDWQMSGK PLLLYLDNAA EFKSEALRRG CEQHGIRLDY RPLGQPHYGG IVERIIGTAM QMIHDELPGT TFSNPDQRGD YDSENKAALT LRELERWLTL AVGTYHGSVH NGLLQPPAAR WAEAVARVGV PAVVTRATSF LVDFLPILRR TLTRTGFVID HIHYYADALK PWIARRERWP SFLIRRDPRD ISRIWVLEPE GQHYLEIPYR TLSHPAVTLW EQRQALAKLR QQGREQVDES ALFRMIGQMR EIVTSAQKAT RKARRDADRR QHLKTSARPD KPVPPDTDIA DPQADNLPPA KPFDQIEEW |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tniB delta1 | TniB delta1 | In21 | 286 | 6457-7317 | + |
|---|
| Class: | Accessory Gene | ||||||||||
| Function: | probable ATP-binding protein. | ||||||||||
| Comment: | probably truncated by insertion of IS1326::IS1353 | ||||||||||
| Protein Sequence: | MDEYPIIDLS HLLPAAQGLA RLPADERIQR LRADRWIGYP RAVEALNRLE ALYAWPNKQR MPNLLLVGPT NNGKSMIVEK FRRTHPASSD ADQEHIPVLV VQMPSEPSVI RFYVALLAAM GAPLRPRPRL PEMEQLALAL LRKVGVRMLV IDELHNVLAG NSVNRREFLN LLRFLGNELR IPLVGVGTRD AYLAIRSDDQ LENRFEPMML PVWEANDDCC SLLASFAASL PLRRPSPIAT LDMARYLLTR SEGTIGELAH LLMAAAIVAV ESGEEAINHR TLSMAC |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnp | Tnp | IS1353 | 514 | 7368-8912 | - |
|---|
| Class: | Transposase | ||||||||||
| Function: | transposase | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Protein Sequence: | MYSYEDRLRA VRLYLKLGRR MSATLRQLGY PTKNSLKAWL AEFERNQDLR RGYQRIKRQY TDEQKQRAVD HYIEQGYCLS HTIRSLGYPS REALRAWIRD LRPEFARTVV GSSAPTVARS RLEKQQAVIA LNLRVGSAKD VADTVGVSRP TLYNWQHRLL GKVPLKPMTK KKGDTSLEQR HEALLRELAE LESQNQRLRM ENAILEKASE LIKKDMGINP LELTSREKTK VVDALRVTFP LANLLCGLKL ARSTYFYQRL RQTRPDKYTQ VREVIRTIFE DNYRCYGYRR IDSALRLGGM RVSEKVVRRL MAQERLVVRT PRRRRFSAYA GDPTPAVPNL LNRDFHASAP NTKWLTDLTE IHIPAGKVYV SPIVDCFDGL VVAWNIGTSP DANLVNTMLD HAVRTLRPGE HPVIHSDRGS HYRWPAWIRR TENAQLTRSM SKKGCSPDNA ACEGFFGRLK TELIYPRNWQ HVTLKDLMTR IDAYIHWYNE RRIKVSLGGR SPIEYRHAVG LMSV |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | istA N-ter | IstA N-ter | IS1326_IS1353_ISEc37.1 | 273 | 9044-9865 | + |
|---|
| Class: | Transposase | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Protein Sequence: | MAILSAIRRW HFRDGASIRE IARRSGLSRN TVRKYLQSKV VEPQYPARDS VGKLSPFEPK LRQWLSTEHK KTKKLRRNLR SMYRDLVALG FTGSYDRVCA FARQWKDSEQ FKAQTSGKGC FIPLRFACGE AFQFDWSEDF ARIAGKQVKL QIAQFKLAHS RAFVLRAYYQ QKHEMLFDAH WHAFQIFGGI PKRGIYDNMK TAVDSVGRGK ERRVNQRFTA MVSHYLFDAQ FCNPASGWEK GQIEKNVQDS RQRLWQGAPD FQSLADLNVW LDR |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnp | Tnp | ISEc37.1 | 395 | 10046-11233 | - |
|---|
| Class: | Transposase | ||||||||||
| Transposase Chemistry: | HUH | ||||||||||
| Protein Sequence: | MLPRFADIFQ QGNRWLNWLE KQPEGAVRPV VIESVTKIMA CGTTLMGYTQ WCCSSPDCCH TKKVCFRCKS RSCPHCGVKA GAQWIQYLLS LVPDCPWQHI VFTLPCQYWS LVFHNRWLLT EMSRIAADVI LEICHQADVE PGIFTVIHTW GRDQQWHPHI HLSTTAGGVT SGHTWKNLHF YARKVMSMWR YRITRLLSRK YPDLVMPDAL AAEGSSKREW NRFLDTHYRR GWNVNVSRVM DNATHVAVYF GSYLKKPPVP MSRLEHYAGQ DEIGLRYNSH RTKREEYLLM SGDEFMERFS WHVADKGFRM VRYYGFLSPA KRRLLEEVVY IITETVRKTA MQITWRGMYQ RLLKVDPLKC VLCGSQMRFT GLKRGYRLAE QVLMHELLAR MRWCG |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | orf2 | Orf2 | ISEc37.1 | 121 | 11233-11598 | - |
|---|
| Class: | Accessory Gene | ||||||||||
| Protein Sequence: | MARSAKPRKR KPASQRSKLP RYVVKLHEDD FFDEEDAEVL RFDSFDDAVE CCADLNIPFF VDAGNKKLVF WFVRVDDEGY PEIARCTERE FATILSGISA GGMYCPECGT VHWPDGVAPP F |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | istA C-ter | IstA C-ter | IS1326_IS1353_ISEc37.1 | 233 | 11687-12387 | + |
|---|
| Class: | Transposase | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Protein Sequence: | SIAAKRCGLS CATPNWTKPC KRPLPMNKAS *WRYPMPLMH SWSKPSESLQ PALFTTRAIA TAFLPVTPTG PSAFGFMQTS W*WLPKANTL PSIQDCLAVA TLGVATHNTT GTITCLCFRR NLGRCAMVRH LLNCHPRSRS FNPSCCNAPA VTVTWWKFWP LYCTTMKVRY SVLWNWHWSV ASHRRSMCLI CWDV*PKNLH PNRFQFPRG* G*HWNHRPT* TAMTV*GEPM MQH |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | istB | IstB | IS1326_IS1353_ISEc37.1 | 261 | 12374-13159 | + |
|---|
| Class: | Accessory Gene | ||||||||||
| Subclass: | ATPase Transposition Helper | ||||||||||
| Function: | stimulates transposition | ||||||||||
| Protein Sequence: | MMQHEGHVRI LKSLKLFGMA HAIEELGNQN SPAFNQALPM LDSLIKAEVA EREVRSVNYQ LRVAKFPVYR DLVGFDFSQS LVNEATVKQL HRCDFMEQAQ NVVLIGGPGT GKTHLATAIG TQAVMHLNRR VRFFSTVDLV NALEQEKSSG RQGQIANRLL YADLVILDEL GYLPFSQTGG ALLFHLLSKL YEKTSVILTT NLSFSEWSRV FGDEKMTTAL LDRLTHHCHI LETGNESYRF KHSSTQNKQE EKQTRKLKIE T |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | GNAT_fam | GNAT_fam | In21 | 166 | 13335-13835 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antibiotic Resistance | ||||||||||
| Sequence Family: | Acetyltransf_1 (Pfam:PF00583) | ||||||||||
| Target: | aminoglycoside antibiotic (ARO:0000016) | ||||||||||
| Comment: | putative acetyltransferase ADU64769.1 | ||||||||||
| Protein Sequence: | MDSEEPPNVR VACSGDIDEV VRLMHDAAAW MSAKGTPAWD VARIDRTFAE TFVLRSELLV ASCSDGIVGC CTLSAEDPEF WPDALKGEAA YLHKLAVRRT HAGRGVSSAL IEACRHAART QGCAKLRLDC HPNLRGLYER LGFTHVDTFN PGWDPTFIAE RLELEI |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | sul1 (ARO:3000410) | Sul1 | In21 | 279 | 13963-14802 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antibiotic Resistance | ||||||||||
| Function: | antibiotic target replacement (ARO:0001002) | ||||||||||
| Sequence Family: | sulfonamide resistant sul (ARO:3004238) | ||||||||||
| Transposase Chemistry: | dihydropteroate synthase | ||||||||||
| Target: | sulfonamide antibiotic (ARO:3000282)||sulfone antibiotic (ARO:3003401) | ||||||||||
| Comment: | perfect match to reference sequence for ARO:3000410 | ||||||||||
| Protein Sequence: | MVTVFGILNL TEDSFFDESR RLDPAGAVTA AIEMLRVGSD VVDVGPAASH PDARPVSPAD EIRRIAPLLD ALSDQMHRVS IDSFQPETQR YALKRGVGYL NDIQGFPDPA LYPDIAEADC RLVVMHSAQR DGIATRTGHL RPEDALDEIV RFFEARVSAL RRSGVAADRL ILDPGMGFFL SPAPETSLHV LSNLQKLKSA LGLPLLVSVS RKSFLGATVG LPVKDLGPAS LAAELHAIGN GADYVRTHAP GDLRSAITFS ETLAKFRSRD ARDRGLDHA |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | qacEdelta1 (ARO:3005010) | QacEdelta1 | In21 | 115 | 14796-15143 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antibiotic Resistance | ||||||||||
| Function: | antibiotic efflux (ARO:0010000) | ||||||||||
| Sequence Family: | major facilitator superfamily (MFS) antibiotic efflux pump (ARO:0010002) | ||||||||||
| Target: | disinfecting agents and antiseptics(ARO:3005386) | ||||||||||
| Comment: | subunit of the qac multidrug efflux pump||perfect match to reference sequence for ARO:3005010 (bitscore:219) | ||||||||||
| Protein Sequence: | MKGWLFLVIA IVGEVIATSA LKSSEGFTKL APSAVVIIGY GIAFYFLSLV LKSIPVGVAY AVWSGLGVVI ITAIAWLLHG QKLDAWGFVG MGLIIAAFLL ARSPSWKSLR RPTPW |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | catB2 (ARO:3002675) | CatB2 | In21 | 210 | 15312-15944 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antibiotic Resistance | ||||||||||
| Function: | antibiotic inactivation (ARO:0001004) | ||||||||||
| Sequence Family: | chloramphenicol acetyltransferase (CAT) (ARO:3000122) | ||||||||||
| Target: | phenicol antibiotic (ARO:3000387) | ||||||||||
| Comment: | 99% match toreference sequence for ARO:3002675 (bitscore: 436) | ||||||||||
| Protein Sequence: | MTNYFESPFK GKLLTEQVKN PNIKVGRYSY YSGYYHGHSF DDCARYLLPD RDDVDQLIIG SFCSIGSGAA FIMAGNQGHR YDWVSSFPFF YMNEEPAFAK SVDAFQRAGD TVIGSDVWIG SEAMIMPGIK IGHGAVIGSR ALVAKDVEPY TIVGGNPAKS IRKRFSEEEI SMLLDMAWWD WPLEQIKEAM PFLCSSGIAS LYRRWQGTSA |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | orfM | OrfM | In21 | 87 | 16093-16356 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Hypothetical | ||||||||||
| Comment: | AAC14736.1 | ||||||||||
| Protein Sequence: | MQGGQLHGVA DGAMRHAGDR VGQLQWPAEL EQIVKFGVCR ISLAGLLLAN LVTWQQPDAF YWPVPDNVFL VTQGGSSENN LPRSMVL |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | parE | ParE | In21 | 99 | 16536-16835 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Toxin | ||||||||||
| Sequence Family: | ParE_toxin (Pfam:PF05016) | ||||||||||
| Target: | DNA gyrase | ||||||||||
| Comment: | WP_115197934.1 | ||||||||||
| Protein Sequence: | MELRIAESTR GDLQAIQEYY RAEGVPQIGD DFVAATLGHC EMLQRHPEAG RVVPEFNLDN IRELIHAPFR VVYLRQSTET VLIRIWCSER QLELPENEE |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | phd | Phd | In21 | 89 | 16836-17105 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antitoxin | ||||||||||
| Sequence Family: | PhdYeFM_antitox (Pfam:PF02604) | ||||||||||
| Comment: | Phd/YeFM | ||||||||||
| Protein Sequence: | MQVKFSEDVI PLSDLKINPG KVVNRTQDTQ RPILLTSRGR GVAVVQGLED YERAAEELRF VKAVAQGLVD VREGSTVSLE DARKALGIE |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | orfJ | OrfJ | In21 | 85 | 17269-17526 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Hypothetical | ||||||||||
| Comment: | WP_115197932.1 | ||||||||||
| Protein Sequence: | MHDFNGAQIF VGAKVKVLFI DQTIMNGLPS SEVNDINSML GEIFEVYEID EYGQAWVEKW WHLDEGKSYS HSLGLSSTEL ELVEK |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | orfI | OrfI | In21 | 192 | 17558-18136 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Hypothetical | ||||||||||
| Comment: | WP_115197931.1 | ||||||||||
| Protein Sequence: | MPRIFTQLIA LLLLVACKPE SVPLNESFTC SENPIELEQI SQLLKNEHVP FTQETVLVDR VSFTSAGVKS VLPKGATCIF YSHKYSNITN KINKEVIDDF PPIWGRATSW DDRNAQLVEK LSQHGIQTTR RVYRGKEWIA WPIEDVELAE NILGFNEKMK QYFKEQRLKM YAPNQALKAQ PSATVDAASS AS |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | orfH | OrfH | In21 | 110 | 18257-18589 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Hypothetical | ||||||||||
| Comment: | WP_115197930.1 | ||||||||||
| Protein Sequence: | MGNFIKTLVC VVSAIFAVLA IDALISGNVR DSITLLVISA FVALCGGIGL FFQRFPSPPL APGEGNSAWT LLRSYRKQYP GWQGKLVYYG IPTLLLIVML FRLSELLASI |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | orfG | OrfG | In21 | 141 | 18727-19152 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Hypothetical | ||||||||||
| Comment: | WP_013136948.1 | ||||||||||
| Protein Sequence: | MEQINNIELV DPSIYPTDEI LKRILGKSFS VYLKLLRLYE NYSLIPEWKY YKDGKAWLCK VIKGKKTIVW MSAWKNYIKA TIYLPEKHIN GVLVLDIHEI TKKAFIETNN IGRSRPCMFE LKEENILEDF IKVMQFKMTL K |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | AAC(6')-Ia (ARO:3002545) | AAC(6')-Ia | In21 | 185 | 19157-19714 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antibiotic Resistance | ||||||||||
| Function: | antibiotic inactivation (ARO:0001004) | ||||||||||
| Sequence Family: | AAC(6') (ARO:3000345) | ||||||||||
| Target: | aminoglycoside antibiotic (ARO:0000016) | ||||||||||
| Comment: | strict match to reference sequence for ARO:3002545 (bitscore: 378) | ||||||||||
| Protein Sequence: | MNYQIVNIAE CSNYQLEAAN ILTEAFNDLG NNSWPDMTSA TKEVKECIES PNLCFGLLIN NSLVGWIGLR PMYKETWELH PLVVRPDYQN KGIGKILLKE LENRAREQGI IGIALGTDDE YYRTSLSLIT ITEDNIFDSI KNIKNINKHP YEFYQKNGYY IVGIIPNANG KNKPDIWMWK SLIKE |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | aadA3 (ARO:3002603) | AadA3 | In21 | 263 | 19814-20605 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antibiotic Resistance | ||||||||||
| Function: | antibiotic inactivation (ARO:0001004) | ||||||||||
| Sequence Family: | ANT(3'')(ARO:3004275) | ||||||||||
| Target: | aminoglycoside antibiotic (ARO:0000016) | ||||||||||
| Comment: | strict match to reference sequence for ARO:3002603 (bitscore: 530) | ||||||||||
| Protein Sequence: | MRVAVTIEIS NQLSEVLSVI ERHLESTLLA VHLYGSAVDG GLKPYSDIDL LVTVAVKLDE TTRRALLNDL MEASAFPGES ETLRAIEVTL VVHDDIIPWR YPAKRELQFG EWQRNDILAG IFEPAMIDID LAILLTKARE HSVALVGPAA EEFFDPVPEQ DLFEALRETL KLWNSQPDWA GDERNVVLTL SRIWYSAITG KIAPKDVAAD WAIKRLPAQY QPVLLEAKQA YLGQKEDHLA SRADHLEEFI HYVKGEITKV VGK |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | intI1 | IntI1 | In21 | 337 | 20754-21767 | + |
|---|
| Class: | Integron Integrase | ||||||||||
| Subclass: | Class 1 | ||||||||||
| Sequence Family: | Class 1 Integron Tyrosine Integrase | ||||||||||
| Transposase Chemistry: | Tyrosine | ||||||||||
| Protein Sequence: | MKTATAPLPP LRSVKVLDQL RERIRYLHYS LRTEQAYVHW VRAFIRFHGV RHPATLGSSE VEAFLSWLAN ERKVSVSTHR QALAALLFFY GKVLCTDLPW LQEIGRPRPS RRLPVVLTPD EVVRILGFLE GEHRLFAQLL YGTGMRISEG LQLRVKDLDF DHGTIIVREG KGSKDRALML PESLAPSLRE QLSRARAWWL KDQAEGRSGV ALPDALERKY PRAGHSWPWF WVFAQHTHST DPRSGVVRRH HMYDQTFQRA FKRAVEQAGI TKPATPHTLR HSFATALLRS GYDIRTVQDL LGHSDVSTTM IYTHVLKVGG AGVRSPLDAL PPLTSER |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpM | TnpM | Tn2424 | 116 | 21970-22320 | + |
|---|
| Class: | Accessory Gene | ||||||||||
| Subclass: | Inhibitor | ||||||||||
| Function: | transposition regulator||reported to enhance Tn21 transposition and suppress resolution of cointegrate replicons in vivo | ||||||||||
| Comment: | 3'-end of urfM ORF, which is interrupted by insertion of In2||inhibits tranposition probably by inhibiting resolution | ||||||||||
| Protein Sequence: | MEVVAEGVET PDCLAWLRQA GCDTVQGFLF ARPMPAAAFV GFVNQWRNTT MNANEPSTSC CVCCKEIPLD AAFTPEGAEY VEHFCGLECY QRFQARASTA TETSVKPDAC DSPPSG |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpR | TnpR | Tn2424 | 186 | 22446-23006 | + |
|---|
| Class: | Accessory Gene | ||||||||||
| Subclass: | Resolvase | ||||||||||
| Function: | resolvase||serine site-specific recombinase | ||||||||||
| Sequence Family: | Serine Site-Specific Recombinase | ||||||||||
| Transposase Chemistry: | Serine | ||||||||||
| Comment: | identical to tnpR (TnAs3 ) | ||||||||||
| Protein Sequence: | MTGQRIGYIR VSTFDQNPER QLEGVKVDRA FSDKASGKDV KRPQLEALIS FARTGDTVVV HSMDRLARNL DDLRRIVQTL TQRGVHIEFV KEHLSFTGED SPMANLMLSV MGAFAEFERA LIRERQREGI ALAKQRGAYR GRKKSLSSER IAELRQRVEA GEQKTKLARE FGISRETLYQ YLRTDQ |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpA | TnpA | Tn2424 | 988 | 23009-25975 | + |
|---|
| Class: | Transposase | ||||||||||
| Function: | transposition, DNA-mediated (GO:0006313) | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Comment: | identical to TnAs3 tnpA | ||||||||||
| Protein Sequence: | MPRRSILSAA ERESLLALPD SKDDLIRHYT FNDTDLSIIR QRRGPANRLG FAVQLCYLRF PGVILGVDEL PFPPLLKLVA DQLKVGVESW NEYGQREQTR REHLSELQTV FGFRPFTMSH YRQAVQMLTE LAMQTDKGIV LASALIGHLR RQSVILPALN AVERASAEAI TRANRRIYDA LAEPLADAHR RRLDDLLKRR DNGKTTWLAW LRQSPAKPNS RHMLEHIERL KAWQALDLPT GIERLVHQNR LLKIAREGGQ MTPADLAKFE PQRRYATLVA LATEGMATVT DEIIDLHDRI LGKLFNAAKN KHQQQFQASG KAINAKVRLY GRIGQALIDA KQSGRDAFAA IEAVMSWDSF AESVTEAQKL AQPDDFDFLH RIGESYATLR RYAPEFLAVL KLRAAPAAKN VLDAIEVLRG MNTDNARKLP ADAPTGFIKP RWQKLVMTDA GIDRRYYELC ALSELKNSLR SGDIWVQGSR QFKDFEDYLV PPEKFTSLKQ SSELPLAVAT DCEQYLHERL TLLEAQLATV NRMAAANDLP DAIITESGLK ITPLDAAVPD TAQALIDQTA MVLPHVKITE LLLEVDEWTG FTRHFTHLKS GDLAKDKNLL LTTILADAIN LGLTKMAESC PGTTYAKLAW LQAWHTRDET YSTALAELVN AQFRHPFAGH WGDGTTSSSD GQNFRTASKA KSTGHINPKY GSSPGRTFYT HISDQYAPFH TKVVNVGLRD STYVLDGLLY HESDLRIEEH YTDTAGFTDH VFALMHLLGF RFAPRIRDLG DTKLYIPKGD AAYDALKPMI GGTLNIKHVR AHWDEILRLA TSIKQGTVTA SLMLRKLGSY PRQNGLAVAL RELGRIERTL FILDWLQSVE LRRRVHAGLN KGEARNALAR AVFFNRLGEI RDRSFEQQRY RASGLNLVTA AIVLWNTVYL ERAAHALRGN GHAVDDSLLQ YLSPLGWEHI NLTGDYLWRS SAKIGAGKFR PLRPLQPA |
||||||||||
| TnCentral Accession | TE Name | Type | Coordinates | Strand | Length |
|---|---|---|---|---|---|
| In21-UGCJ01000005 | In21 | integron | 4634-21969 | + | 17336 |
| IS1326_IS1353_ISEc37.1-UGCJ01000005 | IS1326_IS1353_ISEc37.1 | insertion sequence | 7312-13226 | + | 5915 |
| IS1353-AF071413 | IS1353 | insertion sequence | 7340-8953 | + | 1614 |
| ISEc37.1-UGCJ01000005 | ISEc37.1 | transposon | 9859-11687 | + | 1829 |
| Name | Associated TE | Coordinates | Direction | Length | Sequence |
|---|---|---|---|---|---|
| IRt | In21 | 4634-4666 | + | 33 | TGTCATTTTC AGAAGACGAC TGCACCAGTT GAT |
| repeat t1 | In21 | 4642-4660 | + | 19 | TCAGAAGACG ACTGCACCA |
| repeat t2 | In21 | 4682-4700 | - | 19 | TCAGGAGCTG GCTGCACAA |
| repeat t3 | In21 | 4711-4730 | + | 20 | TCAGAAGTGA TCTGCACCAA |
| repeat t4 | In21 | 4743-4761 | + | 19 | TCAATACTCG TGTGCACCA |
| IRL | IS1326_IS1353_ISEc37.1 | 7312-7337 | + | 26 | TGTTGAGTTG CATCTAAAAT TGACCC |
| IRR | IS1353 | 7340-7352 | + | 13 | TGGGGGTGCG GAC |
| IRL | IS1353 | 8942-8953 | - | 12 | TGGGGTGCGG AC |
| IRR | IS1326_IS1353_ISEc37.1 | 13201-13226 | - | 26 | TGTTGATTTG CACCCAAATT TGACCC |
| repeat i4 | In21 | 21850-21868 | - | 19 | TCAGCGGACG CAGGGAGGA |
| repeat i3 | In21 | 21878-21896 | - | 19 | TCAGGCAACG ACGGGCTGC |
| repeat i2 | In21 | 21920-21938 | - | 19 | TCAGAAGCCG ACTGCACTA |
| IRi | In21 | 21937-21969 | - | 33 | TGTCGTTTTC AGAAGACGGC TGCACTGAAC GTC |
| repeat i1 | In21 | 21943-21961 | - | 19 | TCAGAAGACG GCTGCACTG |
1. Meyer JF, Nies BA, Wiedemann B.
Amikacin resistance mediated by multiresistance transposon Tn2424.. J Bacteriol. 1983 Aug;155(2):755-60. doi: 10.1128/jb.155.2.755-760.1983. PubMed ID:
6307980
.
2. Parent R, Roy PH. The chloramphenicol acetyltransferase gene of Tn2424: a new breed of cat.. J Bacteriol. 1992 May;174(9):2891-7. doi: 10.1128/jb.174.9.2891-2897.1992. PubMed ID: 1314803 .
2. Parent R, Roy PH. The chloramphenicol acetyltransferase gene of Tn2424: a new breed of cat.. J Bacteriol. 1992 May;174(9):2891-7. doi: 10.1128/jb.174.9.2891-2897.1992. PubMed ID: 1314803 .