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Mobile element type:  Transposon
Name:  Tn21
Synonyms: 
Accession:  Tn21-AF071413
Family:  Tn3
Group:  Tn21
First isolate:  Yes
Partial:  ND
Evidence of transposition:  Yes

Host Organism:  Shigella flexneri
Date of Isolation:  1950s
Country:  Japan
Molecular Source:  plasmid NR1 (R100)

Name Coordinates Direction Length Sequence
IRL 1-38 + 38 GGGGGCACCT CAGAAAACGG AAAATAAAGC ACGCTAAG
IRR 19635-19672 - 38 GGGGTCGTCT CAGAAAACGG AAAATAAAGC ACGCTAAG
repeat Tn21 19645-19663 - 19 TCAGAAAACG GAAAATAAA

Name Associated TE Coordinates Orientation Length Sequence
attC-qacE2 3'-end In2 7323-7328 + 6 ATCTAA
attC-qacE2 3'-end In2 11506-11511 + 6 TTAGAT
attC qacEdelta1_sul1 In2 12106-12139 , 13417-13423 + 41 CCGCTAGCGG GCGGCCGGAA GGTGAATGCT AGGCATCTAA C
attC qacEdelta1_sul1 core In2 12106-12139 + 34 CCGCTAGCGG GCGGCCGGAA GGTGAATGCT AGGC
attI In2 14279-14334 + 56 CTTTGTTTTA GGGCGACTGC CCTGCTGCGT AACATCGTTG CTGCTCCATA ACATCA
res Tn21 15969-16099 + 131 GCCGCCGTCA GGTTGAGGCA TACCCTAACC TGATGTCAGA TGCCATGTGT AAATTGCGTC AGGATAGGAT TGAATTTTGA ATTTATTGAC ATATCTCGTT GAAGGTCATA GAGTCTTCCC TGACATTTTG C
res_site_I Tn21 15969-16007 + 39 GCCGCCGTCA GGTTGAGGCA TACCCTAACC TGATGTCAG
res_site_II Tn21 16021-16064 + 44 ATTGCGTCAG GATAGGATTG AATTTTGAAT TTATTGACAT ATCT
res_site_III Tn21 16068-16099 + 32 TGAAGGTCAT AGAGTCTTCC CTGACATTTT GC

ORF Summary
Name Associated TE Coordinates Orientation Class Subclass
merR Tn21 34-468 - Passenger Gene Heavy Metal Resistance
merT Tn21 540-890 + Passenger Gene Heavy Metal Resistance
merP Tn21 904-1179 + Passenger Gene Heavy Metal Resistance
merC Tn21 1215-1637 + Passenger Gene Heavy Metal Resistance
merA Tn21 1689-3383 + Passenger Gene Heavy Metal Resistance
merD Tn21 3401-3763 + Passenger Gene Heavy Metal Resistance
merE Tn21 3760-3996 + Passenger Gene Heavy Metal Resistance
urfM 5'-end Tn21 3993-4663 + Passenger Gene Other
urfM 5'-end Tn21 3993-4663 + Passenger Gene Other
tniA In2 4775-6454 + Transposase
tniB delta1 In2 6457-7317 + Accessory Gene
tnp IS1353 7368-8912 - Transposase
istA IS1326::IS1353 9035-10558 + Transposase None
istB IS1326::IS1353 10545-11330 + Accessory Gene ATPase Transposition Helper
GNAT_fam In2 11506-12006 - Passenger Gene Antibiotic Resistance
sul1 (ARO:3000410) In2 12134-12973 - Passenger Gene Antibiotic Resistance
qacEdelta1 (ARO:3005010) In2 12967-13314 - Passenger Gene Antibiotic Resistance
aadA (ARO:3002601) In2 13478-14269 - Passenger Gene Antibiotic Resistance
intI1 In2 14418-15431 + Integron Integrase Class 1
tnpM Tn21 15634-15984 + Accessory Gene Inhibitor
tnpR Tn21 16110-16670 + Accessory Gene Resolvase
tnpA Tn21 16673-19639 + Transposase
ORF Details
Gene Name Protein Name Associated TE Gene Length Coordinates Strand
merR MerR Tn21 144 34-468 -
Class: Passenger Gene
Subclass: Heavy Metal Resistance
Function: activator-repressor of mer operon
Target: Mercury
Protein Sequence: MENNLENLTI GVFAKAAGVN VETIRFYQRK GLLREPDKPY GSIRRYGEAD VVRVKFVKSA QRLGFSLDEI AELLRLDDGT HCEEASSLAE HKLKDVREKM ADLARMETVL SELVCACHAR KGNVSCPLIA SLQGEAGLAR SAMP
Gene Name Protein Name Associated TE Gene Length Coordinates Strand
merT MerT Tn21 116 540-890 +
Class: Passenger Gene
Subclass: Heavy Metal Resistance
Function: cytosolic mercuric ion transport protein
Target: Mercury
Protein Sequence: MSEPQNGRGA LFAGGLAAIL ASTCCLGPLV LVALGFSGAW IGNLTVLEPY RPLFIGAALV ALFFAWKRIY RPVQACKPGE VCAIPQVRAT YKLIFWIVAV LVLVALGFPY VVPFFY
Gene Name Protein Name Associated TE Gene Length Coordinates Strand
merP MerP Tn21 91 904-1179 +
Class: Passenger Gene
Subclass: Heavy Metal Resistance
Function: mercury transport
Target: Mercury
Protein Sequence: MKKLFASLAL AAAVAPVWAA TQTVTLAVPG MTCAACPITV KKALSKVEGV SKVDVGFEKR EAVVTFDDTK ASVQKLTKAT ADAGYPSSVK Q
Gene Name Protein Name Associated TE Gene Length Coordinates Strand
merC MerC Tn21 140 1215-1637 +
Class: Passenger Gene
Subclass: Heavy Metal Resistance
Function: transmembrane protein mercury transport
Target: Mercury
Protein Sequence: MGLMTRIADK TGALGSVVSA MGCAACFPAL ASFGAAIGLG FLSQYEGLFI SRLLPLFAAL AFLANALGWF SHRQWLRSLL GMIGPAIVFA ATVWLLGNWW TANLMYVGLA LMIGVSIWDF VSPAHRRCGP DGCELPAKRL
Gene Name Protein Name Associated TE Gene Length Coordinates Strand
merA MerA Tn21 564 1689-3383 +
Class: Passenger Gene
Subclass: Heavy Metal Resistance
Function: mercuric ion reductase
Target: Mercury
Protein Sequence: MSTLKITGMT CDSCAVHVKD ALEKVPGVQS ADVSYAKGSA KLAIEVGTSP DALTAAVAGL GYRATLADAP SVSTPGGLLD KMRDLLGRND KTGSSGALHI AVIGSGGAAM AAALKAVEQG ARVTLIERGT IGGTCVNVGC VPSKIMIRAA HIAHLRRESP FDGGIAATTP TIQRTALLAQ QQARVDELRH AKYEGILEGN PAITVLHGSA RFKDNRNLIV QLNDGGERVV AFDRCLIATG ASPAVPPIPG LKDTPYWTST EALVSETIPK RLAVIGSSVV ALELAQAFAR LGAKVTILAR STLFFREDPA IGEAVTAAFR MEGIEVREHT QASQVAYING EGDGEFVLTT AHGELRADKL LVATGRAPNT RKLALDATGV TLTPQGAIVI DPGMRTSVEH IYAAGDCTDQ PQFVYVAAAA GTRAAINMTG GDAALNLTAM PAVVFTDPQV ATVGYSEAEA HHDGIKTDSR TLTLDNVPRA LANFDTRGFI KLVVEEGSGR LIGVQAVAPE AGELIQTAAL AIRNRMTVQE LADQLFPYLT MVEGLKLAAQ TFNKDVKQLS CCAG
Gene Name Protein Name Associated TE Gene Length Coordinates Strand
merD MerD Tn21 120 3401-3763 +
Class: Passenger Gene
Subclass: Heavy Metal Resistance
Function: secondary regulatory protein
Target: Mercury
Protein Sequence: MSAYTVSQLA HNAGVSVHIV RDYLVRGLLR PVACTTGGYG VFDDAALQRL CFVRAAFEAG IGLDALARLC RALDAADGAQ AAAQLAVLRQ LVERRRAALA HLDAQLASMP AERAHEEALP
Gene Name Protein Name Associated TE Gene Length Coordinates Strand
merE MerE Tn21 78 3760-3996 +
Class: Passenger Gene
Subclass: Heavy Metal Resistance
Function: mercury transport
Target: Mercury
Comment: similar to urf-1 in pKLH2 (GenBank AF213017), pKLH272 (Genbank Y08992), pMER610 (GenBank Y08993), pKLH210 (GenBank Y10102), Tn5036 (Genbank Y09025), orf1 in Tn501 (GenBank Z00027), and urf-1 in Tn5041 (GenBank X98999)
Protein Sequence: MNAPDKLPPE TRQPVSGYLW GALAVLTCPC HLPILAAVLA GTTAGAFLGE HWGVAALALT GLFVLAVTRL LRAFRGGS
Gene Name Protein Name Associated TE Gene Length Coordinates Strand
urfM 5'-end N/A Tn21 223 3993-4663 +
Class: Passenger Gene
Subclass: Other
Comment: urfMORF interrupted by insertion of In2
Protein Sequence: MTSSQPAGWT AAELAQAAAR GQLDLHYQPL VDLRDHRIAG AEALMRWRHP RLGLLPPGQF LPLAESFGLM PEIGAWVLGE ACRQMHKWQG PAWQPFRLAI NVSASQVGPT FDDEVKRVLA DMALPAELLE IELTESVAFG NPALFASFDA LRAIGVRFAA DDFGTGYSCL QHLKCCPITT LKIDQSFVAR LPDDARDQTI VRAVIQLAHG LGMDVIFRRR LHQ
Gene Name Protein Name Associated TE Gene Length Coordinates Strand
urfM 5'-end N/A Tn21 223 3993-4663 +
Class: Passenger Gene
Subclass: Other
Comment: urfMORF interrupted by insertion of In2
Protein Sequence: MTSSQPAGWT AAELAQAAAR GQLDLHYQPL VDLRDHRIAG AEALMRWRHP RLGLLPPGQF LPLAESFGLM PEIGAWVLGE ACRQMHKWQG PAWQPFRLAI NVSASQVGPT FDDEVKRVLA DMALPAELLE IELTESVAFG NPALFASFDA LRAIGVRFAA DDFGTGYSCL QHLKCCPITT LKIDQSFVAR LPDDARDQTI VRAVIQLAHG LGMDVIFRRR LHQ
Gene Name Protein Name Associated TE Gene Length Coordinates Strand
tniA TniA In2 559 4775-6454 +
Class: Transposase
Transposase Chemistry: DDE
Comment: can be extended upstream by 12 amino acids| identical to tniA (Tn1721 and In2)| 25% amino acid sequence identity to TnsB from Tn7
Protein Sequence: MATDTPRIPE QGVATLPDEA WERARRRAEI ISPLAQSETV GHEAADMAAQ ALGLSRRQVY VLIRRARQGS GLVTDLVPGQ SGGGKGKGRL PEPVERVIHE LLQKRFLTKQ KRSLAAFHRE VTQVCKAQKL RVPARNTVAL RIASLDPRKV IRRREGQDAA RDLQGVGGEP PAVTAPLEQV QIDHTVIDLI VVDDRDRQPI GRPYLTLAID VFTRCVLGMV VTLEAPSAVS VGLCLVHVAC DKRPWLEGLN VEMDWQMSGK PLLLYLDNAA EFKSEALRRG CEQHGIRLDY RPLGQPHYGG IVERIIGTAM QMIHDELPGT TFSNPDQRGD YDSENKAALT LRELERWLTL AVGTYHGSVH NGLLQPPAAR WAEAVARVGV PAVVTRATSF LVDFLPILRR TLTRTGFVID HIHYYADALK PWIARRERWP SFLIRRDPRD ISRIWVLEPE GQHYLEIPYR TLSHPAVTLW EQRQALAKLR QQGREQVDES ALFRMIGQMR EIVTSAQKAT RKARRDADRR QHLKTSARPD KPVPPDTDIA DPQADNLPPA KPFDQIEEW
Gene Name Protein Name Associated TE Gene Length Coordinates Strand
tniB delta1 TniB delta1 In2 286 6457-7317 +
Class: Accessory Gene
Function: probable ATP-binding protein.
Comment: probably truncated by insertion of IS1326::IS1353
Protein Sequence: MDEYPIIDLS HLLPAAQGLA RLPADERIQR LRADRWIGYP RAVEALNRLE ALYAWPNKQR MPNLLLVGPT NNGKSMIVEK FRRTHPASSD ADQEHIPVLV VQMPSEPSVI RFYVALLAAM GAPLRPRPRL PEMEQLALAL LRKVGVRMLV IDELHNVLAG NSVNRREFLN LLRFLGNELR IPLVGVGTRD AYLAIRSDDQ LENRFEPMML PVWEANDDCC SLLASFAASL PLRRPSPIAT LDMARYLLTR SEGTIGELAH LLMAAAIVAV ESGEEAINHR TLSMAC
Gene Name Protein Name Associated TE Gene Length Coordinates Strand
tnp Tnp IS1353 514 7368-8912 -
Class: Transposase
Function: transposase
Transposase Chemistry: DDE
Protein Sequence: MYSYEDRLRA VRLYLKLGRR MSATLRQLGY PTKNSLKAWL AEFERNQDLR RGYQRIKRQY TDEQKQRAVD HYIEQGYCLS HTIRSLGYPS REALRAWIRD LRPEFARTVV GSSAPTVARS RLEKQQAVIA LNLRVGSAKD VADTVGVSRP TLYNWQHRLL GKVPLKPMTK KKGDTSLEQR HEALLRELAE LESQNQRLRM ENAILEKASE LIKKDMGINP LELTSREKTK VVDALRVTFP LANLLCGLKL ARSTYFYQRL RQTRPDKYTQ VREVIRTIFE DNYRCYGYRR IDSALRLGGM RVSEKVVRRL MAQERLVVRT PRRRRFSAYA GDPTPAVPNL LNRDFHASAP NTKWLTDLTE IHIPAGKVYV SPIVDCFDGL VVAWNIGTSP DANLVNTMLD HAVRTLRPGE HPVIHSDRGS HYRWPAWIRR TENAQLTRSM SKKGCSPDNA ACEGFFGRLK TELIYPRNWQ HVTLKDLMTR IDAYIHWYNE RRIKVSLGGR SPIEYRHAVG LMSV
Gene Name Protein Name Associated TE Gene Length Coordinates Strand
istA IstA IS1326::IS1353 507 9035-10558 +
Class: Transposase
Transposase Chemistry: DDE
Protein Sequence: MINVAILSAI RRWHFRDGAS IREIARRSGL SRNTVRKYLQ SKVVEPQYPA RDSVGKLSPF EPKLRQWLST EHKKTKKLRR NLRSMYRDLV ALGFTGSYDR VCAFARQWKD SEQFKAQTSG KGCFIPLRFA CGEAFQFDWS EDFARIAGKQ VKLQIAQFKL AHSRAFVLRA YYQQKHEMLF DAHWHAFQIF GGIPKRGIYD NMKTAVDSVG RGKERRVNQR FTAMVSHYLF DAQFCNPASG WEKGQIEKNV QDSRQRLWQG APDFQSLADL NVWLEHRCKA LWSELRHPEL DQTVQEAFAD EQGELMALPN AFDAFVEQTK RVTSTCLVHH EGNRYSVPAS YANRAISLRI YADKLVMAAE GQHIAEHPRL FGSGHARRGH TQYDWHHYLS VLQKKPGALR NGAPFAELPP AFKKLQSILL QRPGGDRDMV EILALVLHHD EGAVLSAVEL ALECGKPSKE HVLNLLGRLT EEPPPKPIPI PKGLRLTLEP QANVNRYDSL RRAHDAA
Gene Name Protein Name Associated TE Gene Length Coordinates Strand
istB IstB IS1326::IS1353 261 10545-11330 +
Class: Accessory Gene
Subclass: ATPase Transposition Helper
Function: stimulates transposition
Protein Sequence: MMQHEGHVRI LKSLKLFGMA HAIEELGNQN SPAFNQALPM LDSLIKAEVA EREVRSVNYQ LRVAKFPVYR DLVGFDFSQS LVNEATVKQL HRCDFMEQAQ NVVLIGGPGT GKTHLATAIG TQAVMHLNRR VRFFSTVDLV NALEQEKSSG RQGQIANRLL YADLVILDEL GYLPFSQTGG ALLFHLLSKL YEKTSVILTT NLSFSEWSRV FGDEKMTTAL LDRLTHHCHI LETGNESYRF KHSSTQNKQE EKQTRKLKIE T
Gene Name Protein Name Associated TE Gene Length Coordinates Strand
GNAT_fam GNAT_fam In2 166 11506-12006 -
Class: Passenger Gene
Subclass: Antibiotic Resistance
Sequence Family: GNAT
Target: aminoglycoside antibiotic (ARO:0000016)
Protein Sequence: MDSEEPPNVR VACSGDIDEV VRLMHDAAAW MSAKGTPAWD VARIDRTFAE TFVLRSELLV ASCSDGIVGC CTLSAEDPEF WPDALKGEAA YLHKLAVRRT HAGRGVSSAL IEACRHAART QGCAKLRLDC HPNLRGLYER LGFTHVDTFN PGWDPTFIAE RLELEI
Gene Name Protein Name Associated TE Gene Length Coordinates Strand
sul1 (ARO:3000410) Sul1 In2 279 12134-12973 -
Class: Passenger Gene
Subclass: Antibiotic Resistance
Function: antibiotic target replacement (ARO:0001002)
Sequence Family: sulfonamide resistant sul (ARO:3004238)
Transposase Chemistry: dihydropteroate synthase
Target: sulfonamide antibiotic (ARO:3000282)||sulfone antibiotic (ARO:3003401)
Comment: perfect match to reference sequence for ARO:3000410
Protein Sequence: MVTVFGILNL TEDSFFDESR RLDPAGAVTA AIEMLRVGSD VVDVGPAASH PDARPVSPAD EIRRIAPLLD ALSDQMHRVS IDSFQPETQR YALKRGVGYL NDIQGFPDPA LYPDIAEADC RLVVMHSAQR DGIATRTGHL RPEDALDEIV RFFEARVSAL RRSGVAADRL ILDPGMGFFL SPAPETSLHV LSNLQKLKSA LGLPLLVSVS RKSFLGATVG LPVKDLGPAS LAAELHAIGN GADYVRTHAP GDLRSAITFS ETLAKFRSRD ARDRGLDHA
Gene Name Protein Name Associated TE Gene Length Coordinates Strand
qacEdelta1 (ARO:3005010) QacEdelta1 In2 115 12967-13314 -
Class: Passenger Gene
Subclass: Antibiotic Resistance
Function: antibiotic efflux (ARO:0010000)
Sequence Family: major facilitator superfamily (MFS) antibiotic efflux pump (ARO:0010002)
Target: disinfecting agents and antiseptics(ARO:3005386)
Comment: subunit of the qac multidrug efflux pump||perfect match to reference sequence for ARO:3005010 (bitscore:219)
Protein Sequence: MKGWLFLVIA IVGEVIATSA LKSSEGFTKL APSAVVIIGY GIAFYFLSLV LKSIPVGVAY AVWSGLGVVI ITAIAWLLHG QKLDAWGFVG MGLIIAAFLL ARSPSWKSLR RPTPW
Gene Name Protein Name Associated TE Gene Length Coordinates Strand
aadA (ARO:3002601) AadA In2 263 13478-14269 -
Class: Passenger Gene
Subclass: Antibiotic Resistance
Function: antibiotic inactivation (ARO:0001004)
Sequence Family: ANT(3'')(ARO:3004275)
Transposase Chemistry: aminoglycoside nucleotidyltransferase
Target: aminoglycoside antibiotic (ARO:0000016)
Comment: perfect match to reference sequence for ARO:3002601||Synonyms: aadA1-pm, aadA, aadA1, aad(3'')(9)
Protein Sequence: MREAVIAEVS TQLSEVVGVI ERHLEPTLLA VHLYGSAVDG GLKPHSDIDL LVTVTVRLDE TTRRALINDL LETSASPGES EILRAVEVTI VVHDDIIPWR YPAKRELQFG EWQRNDILAG IFEPATIDID LAILLTKARE HSVALVGPAA EELFDPVPEQ DLFEALNETL TLWNSPPDWA GDERNVVLTL SRIWYSAVTG KIAPKDVAAD WAMERLPAQY QPVILEARQA YLGQEEDRLA SRADQLEEFV HYVKGEITKV VGK
Gene Name Protein Name Associated TE Gene Length Coordinates Strand
intI1 IntI1 In2 337 14418-15431 +
Class: Integron Integrase
Subclass: Class 1
Sequence Family: Class 1 Integron Tyrosine Integrase
Transposase Chemistry: Tyrosine
Protein Sequence: MKTATAPLPP LRSVKVLDQL RERIRYLHYS LRTEQAYVHW VRAFIRFHGV RHPATLGSSE VEAFLSWLAN ERKVSVSTHR QALAALLFFY GKVLCTDLPW LQEIGRPRPS RRLPVVLTPD EVVRILGFLE GEHRLFAQLL YGTGMRISEG LQLRVKDLDF DHGTIIVREG KGSKDRALML PESLAPSLRE QLSRARAWWL KDQAEGRSGV ALPDALERKY PRAGHSWPWF WVFAQHTHST DPRSGVVRRH HMYDQTFQRA FKRAVEQAGI TKPATPHTLR HSFATALLRS GYDIRTVQDL LGHSDVSTTM IYTHVLKVGG AGVRSPLDAL PPLTSER
Gene Name Protein Name Associated TE Gene Length Coordinates Strand
tnpM TnpM Tn21 116 15634-15984 +
Class: Accessory Gene
Subclass: Inhibitor
Function: transposition regulator||reported to enhance Tn21 transposition and suppress resolution of cointegrate replicons in vivo
Comment: 3'-end of urfM ORF, which is interrupted by insertion of In2||inhibits tranposition probably by inhibiting resolution
Protein Sequence: MEVVAEGVET PDCLAWLRQA GCDTVQGFLF ARPMPAAAFV GFVNQWRNTT MNANEPSTSC CVCCKEIPLD AAFTPEGAEY VEHFCGLECY QRFQARASTA TETSVKPDAC DSPPSG
Gene Name Protein Name Associated TE Gene Length Coordinates Strand
tnpR TnpR Tn21 186 16110-16670 +
Class: Accessory Gene
Subclass: Resolvase
Function: resolvase||serine site-specific recombinase
Sequence Family: Serine Site-Specific Recombinase
Transposase Chemistry: Serine
Comment: identical to tnpR (TnAs3 )
Protein Sequence: MTGQRIGYIR VSTFDQNPER QLEGVKVDRA FSDKASGKDV KRPQLEALIS FARTGDTVVV HSMDRLARNL DDLRRIVQTL TQRGVHIEFV KEHLSFTGED SPMANLMLSV MGAFAEFERA LIRERQREGI ALAKQRGAYR GRKKSLSSER IAELRQRVEA GEQKTKLARE FGISRETLYQ YLRTDQ
Gene Name Protein Name Associated TE Gene Length Coordinates Strand
tnpA TnpA Tn21 988 16673-19639 +
Class: Transposase
Function: transposition, DNA-mediated (GO:0006313)
Transposase Chemistry: DDE
Comment: identical to TnAs3 tnpA
Protein Sequence: MPRRSILSAA ERESLLALPD SKDDLIRHYT FNDTDLSIIR QRRGPANRLG FAVQLCYLRF PGVILGVDEL PFPPLLKLVA DQLKVGVESW NEYGQREQTR REHLSELQTV FGFRPFTMSH YRQAVQMLTE LAMQTDKGIV LASALIGHLR RQSVILPALN AVERASAEAI TRANRRIYDA LAEPLADAHR RRLDDLLKRR DNGKTTWLAW LRQSPAKPNS RHMLEHIERL KAWQALDLPT GIERLVHQNR LLKIAREGGQ MTPADLAKFE PQRRYATLVA LATEGMATVT DEIIDLHDRI LGKLFNAAKN KHQQQFQASG KAINAKVRLY GRIGQALIDA KQSGRDAFAA IEAVMSWDSF AESVTEAQKL AQPDDFDFLH RIGESYATLR RYAPEFLAVL KLRAAPAAKN VLDAIEVLRG MNTDNARKLP ADAPTGFIKP RWQKLVMTDA GIDRRYYELC ALSELKNSLR SGDIWVQGSR QFKDFEDYLV PPEKFTSLKQ SSELPLAVAT DCEQYLHERL TLLEAQLATV NRMAAANDLP DAIITESGLK ITPLDAAVPD TAQALIDQTA MVLPHVKITE LLLEVDEWTG FTRHFTHLKS GDLAKDKNLL LTTILADAIN LGLTKMAESC PGTTYAKLAW LQAWHTRDET YSTALAELVN AQFRHPFAGH WGDGTTSSSD GQNFRTASKA KSTGHINPKY GSSPGRTFYT HISDQYAPFH TKVVNVGLRD STYVLDGLLY HESDLRIEEH YTDTAGFTDH VFALMHLLGF RFAPRIRDLG DTKLYIPKGD AAYDALKPMI GGTLNIKHVR AHWDEILRLA TSIKQGTVTA SLMLRKLGSY PRQNGLAVAL RELGRIERTL FILDWLQSVE LRRRVHAGLN KGEARNALAR AVFFNRLGEI RDRSFEQQRY RASGLNLVTA AIVLWNTVYL ERAAHALRGN GHAVDDSLLQ YLSPLGWEHI NLTGDYLWRS SAKIGAGKFR PLRPLQPA

TnCentral Accession TE Name Type Coordinates Strand Length
In2-AF071413 In2 integron 4634-15633 + 11000
IS1326_IS1353-AF071413 IS1326::IS1353 insertion sequence 7312-11397 + 4086
IS1353-AF071413 IS1353 insertion sequence 7340-8953 + 1614

Name Associated TE Coordinates Direction Length Sequence
IRt In2 4634-4666 + 33 TGTCATTTTC AGAAGACGAC TGCACCAGTT GAT
repeat t1 In2 4642-4660 + 19 TCAGAAGACG ACTGCACCA
repeat t2 In2 4682-4700 - 19 TCAGGAGCTG GCTGCACAA
repeat t3 In2 4711-4730 + 20 TCAGAAGTGA TCTGCACCAA
repeat t4 In2 4743-4761 + 19 TCAATACTCG TGTGCACCA
IRL IS1326::IS1353 7312-7337 + 26 TGTTGAGTTG CATCTAAAAT TGACCC
IRR IS1353 7340-7352 + 13 TGGGGGTGCG GAC
IRL IS1353 8942-8953 - 12 TGGGGTGCGG AC
IRR IS1326::IS1353 11372-11397 - 26 TGTTGATTTG CACCCAAATT TGACCC
repeat i4 In2 15514-15532 - 19 TCAGCGGACG CAGGGAGGA
repeat i3 In2 15542-15560 - 19 TCAGGCAACG ACGGGCTGC
repeat i2 In2 15584-15602 - 19 TCAGAAGCCG ACTGCACTA
IRi In2 15601-15633 - 33 TGTCGTTTTC AGAAGACGGC TGCACTGAAC GTC
repeat i1 In2 15607-15625 - 19 TCAGAAGACG GCTGCACTG

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4.  Zheng ZX, Chandler M, Hipskind R, Clerget M, Caro L.  Dissection of the r-determinant of the plasmid R100.1: the sequence at the extremities of Tn21..  Nucleic Acids Res. 1981 Dec 11;9(23):6265-78. doi: 10.1093/nar/9.23.6265.  PubMed ID: 6275355 .
5.  Barrineau P, Gilbert P, Jackson WJ, Jones CS, Summers AO, Wisdom S.  The DNA sequence of the mercury resistance operon of the IncFII plasmid NR1..  J Mol Appl Genet. 1984;2(6):601-19.  PubMed ID: 6530603 .
6.  Misra TK, Brown NL, Fritzinger DC, Pridmore RD, Barnes WM, Haberstroh L, Silver S.  Mercuric ion-resistance operons of plasmid R100 and transposon Tn501: the beginning of the operon including the regulatory region and the first two structural genes..  Proc Natl Acad Sci U S A. 1984 Oct;81(19):5975-9. doi: 10.1073/pnas.81.19.5975.  PubMed ID: 6091128 .
7.  Misra TK, Brown NL, Haberstroh L, Schmidt A, Goddette D, Silver S.  Mercuric reductase structural genes from plasmid R100 and transposon Tn501: functional domains of the enzyme..  Gene. 1985;34(2-3):253-62. doi: 10.1016/0378-1119(85)90134-9.  PubMed ID: 2989109 .
8.  Rogowsky P, Halford SE, Schmitt R.  Definition of three resolvase binding sites at the res loci of Tn21 and Tn1721..  EMBO J. 1985 Aug;4(8):2135-41. doi: 10.1002/j.1460-2075.1985.tb03904.x.  PubMed ID: 2998784 .
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