Mobile element type:
Transposon
Name:
Tn1696.1
Synonyms:
Accession:
Tn1696.1-CP047309
Family:
Tn3
Group:
Tn21
First isolate:
Yes
Partial:
ND
Evidence of transposition:
Yes
Host Organism:
Citrobacter freundii L75
Date of Isolation:
2019
Country:
China
Molecular Source:
plasmid pCf76
| Name | Coordinates | Direction | Length | Sequence |
|---|---|---|---|---|
| IRL | 1-38 | + | 38 | GGGGTCGTCT CAGAATTCGG AAAATAAAGC ACGCTAAG |
| IRR | 7943-7983 | - | 41 | GGGGTCGTCT CAGAAAACGG AAAATAAAGC ACGCTAAGCC G |
| Name | Associated TE | Coordinates | Orientation | Length | Sequence |
|---|---|---|---|---|---|
| res | Tn1696.1 | 4278-4397 | + | 120 | CGTCAGATTG AGGCATACCC TAACTTGATG TCAGATGCCA TGTGCAAACG ATGTCAGAAT AGAGTTAAAT TTCCTATTGA TTGACATATT CCGTCAAAGG TAATAGATTT CATCCTGACA |
| res_site_I | Tn1696.1 | 4278-4308 | + | 31 | CGTCAGATTG AGGCATACCC TAACTTGATG T |
| res_site_II | Tn1696.1 | 4329-4363 | + | 35 | TGTCAGAATA GAGTTAAATT TCCTATTGAT TGACA |
| res_site_III | Tn1696.1 | 4366-4397 | + | 32 | TTCCGTCAAA GGTAATAGAT TTCATCCTGA CA |
ORF Summary
| Name | Associated TE | Coordinates | Orientation | Class | Subclass |
|---|---|---|---|---|---|
| merR | Tn1696.1 | 34-489 | - | Passenger Gene | Heavy Metal Resistance |
| merT | Tn1696.1 | 561-926 | + | Passenger Gene | Heavy Metal Resistance |
| merP | Tn1696.1 | 942-1217 | + | Passenger Gene | Heavy Metal Resistance |
| merC | Tn1696.1 | 1245-1670 | + | Passenger Gene | Heavy Metal Resistance |
| merA | Tn1696.1 | 1709-3394 | + | Passenger Gene | Heavy Metal Resistance |
| merD | Tn1696.1 | 3412-3777 | + | Passenger Gene | Heavy Metal Resistance |
| merE | Tn1696.1 | 3774-4010 | + | Passenger Gene | Heavy Metal Resistance |
| urf-2Y | Tn1696.1 | 4076-4288 | + | Passenger Gene | Heavy Metal Resistance |
| tnpR | Tn1696.1 | 4418-4975 | + | Accessory Gene | Resolvase |
| tnpA | Tn1696.1 | 4978-7950 | + | Transposase |
ORF Details
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merR | MerR | Tn1696.1 | 151 | 34-489 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Protein Sequence: | MQINFENLTI GVFAKAAGVN VETIRFYQRK GLLPEPDKPY GSIRRYGEAD VTRVRFVKSA QRLGFSLDEI AELLRLEDGT HCEEASGLAE HKLKDVREKM ADLARMEAVL SELVCACHAR KGNVSCPLIA SLQDGTKLAA SARGSHGVTT P |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merT | MerT | Tn1696.1 | 121 | 561-926 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Protein Sequence: | MSEPQKSEPQ NGRGALFAGG LAAILASACC LGPLVLIALG FSGAWIGNLT VLEPYRPIFI GAALVALFFA WRRIYRPAQA CKPGEVCAIP QVRATYKLIF WIVAALVLVS LGFPYVMPFF Y |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merP | MerP | Tn1696.1 | 91 | 942-1217 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Protein Sequence: | MKKLFAALAL AAVVAPVWAA TQTVTLSVPG MTCASCPITV KHALSKVEGV SKTDVSFDKR QAVVTFDDAK TNVQKLTKAT EDAGYPSSLK R |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merC | MerC | Tn1696.1 | 141 | 1245-1670 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Protein Sequence: | MGLITRIAGK TGALGSVVSA MGCAACFPAI ASFGAAIGLG FLSQYEGLFI GILLPMFAGI ALLANAIAWL NHRQWRRTAL GTIGPILVLA AVFLMRAYGW QSGGLLYVGL ALMVGVSVWD FISPAHRRCG PDSCELPEQR G |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merA | MerA | Tn1696.1 | 561 | 1709-3394 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Protein Sequence: | MTTLKITGMT CDSCAAHVKE ALEKVPGVQS ALVSYPKGTA QLAIEAGTSS DALTTAVAGL GYEATLADAP PTDNRAGLLD KMRGWIGAAD KPSGNERPLQ VVVIGSGGAA MAAALKAVEQ GAQVTLIERG TIGGTCVNVG CVPSKIMIRA AHIAHLRRES PFDGGMPPTP PTILRERLLA QQQARVEELR HAKYEGILDG NSAITVLHGE ARFKDDQSLI VSLNEGGERV VMFDRCLVAT GASPAVPPIP GLKESPYWTS TEALASDTIP ERLAVIGSSV VALELAQAFA RLGSKVTALA RNTLFFREDP AIGEAVTAAF RAEGIEVLEH TQASQVAHMD GEFVLTTTHG ELRADKLLVA TGRTPNTRSL ALEAAGVAVN AQGAIVIDKG MRTSSPNIYA AGDCTDQPQF VYVAAAAGTR AAINMTGGDA ALDLTAMPAV VFTDPQVATV GYSEAEAHHD GIETDSRLLT LDNVPRALAN FDTRGFIKLV IEEGSGRLIG VQAVAPEAGE LIQTAVLAIR NRMTVQELAD QLFPYLTMVE GLKLAAQTFS KDVKQLSCCA G |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merD | MerD | Tn1696.1 | 121 | 3412-3777 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Protein Sequence: | MSAYTVSRLA LDAGVSVHIV RDYLLRGLLR PVAYTTGGYG LFDDTALQRL RFVRAAFEAG IGLDALARLC RALDAADGDG ASAQLAVLRQ LVERRREALA SLEMQLAAMP TEPAQHAESL P |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | merE | MerE | Tn1696.1 | 78 | 3774-4010 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Target: | Mercury | ||||||||||
| Protein Sequence: | MNSPEHLPSE THKPITGYLW GALAVLTCPC HLPILAIVLA GTTAGAFIGE HWGIAALTLT GLFVLSVTRL LRAFKGRS |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | urf-2Y | Urf-2Y | Tn1696.1 | 70 | 4076-4288 | + |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Heavy Metal Resistance | ||||||||||
| Function: | response to mercury ion (GO:0046689) | ||||||||||
| Target: | Mercury | ||||||||||
| Comment: | belongs to the mer operon || ProteinID:ACE81802.1 | ||||||||||
| Protein Sequence: | MNANAPNTAS CTTCCVCCKE IPLDAAFTPE GAEYVEHFCG LDCYERFQAR AKAATESDIA PVPGGSQPSD |
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| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpR | TnpR | Tn1696.1 | 185 | 4418-4975 | + |
|---|
| Class: | Accessory Gene | ||||||||||
| Subclass: | Resolvase | ||||||||||
| Sequence Family: | Serine Site-Specific Recombinase | ||||||||||
| Transposase Chemistry: | Serine | ||||||||||
| Protein Sequence: | MQGQRIGYVR VSSFDQNPER QLEGVQVARV FTDKASGKDT QRPELERLLA FVREGDTVVV HSMDRLARNL DDLRRIVQGL TQRGVRMEFV KEGLKFTGED SPMANLMLSV MGAFAEFERA LIRERQREGI VLAKQRGAYR GRKKSLNSEQ IAELKRRVAA GDQKTLVARD FGISRETLYQ YLRED |
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| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpA | TnpA | Tn1696.1 | 990 | 4978-7950 | + |
|---|
| Class: | Transposase | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Protein Sequence: | MPRRSILSAT ERESLLALPD AKDELIRHYT FNETDLSVIR QRRGAANRLG FAVQLCYLRF PGTFLGVDEP PFPPLLRMVA AQLKMPVESW SEYGQREQTR REHLVELQTV FGFKPFTMSH YRQAVHTLTE LALQTDKGIV LASALVENLR RQSIILPAMN AIERASAEAI TRANRRIYAA LTDSLLSPHR QRLDELLKRK DGSKVTWLAW LRQSPAKPNS RHMLEHIERL KSWQALDLPA GIERQVHQNR LLKIAREGGQ MTPADLAKFE VQRRYATLVA LAIEGMATVT DEIIDLHDRI IGKLFNAAKN KHQQQFQASG KAINDKVRMY GRIGQALIEA KQSGSDPFAA IEAVMPWDTF AASVTEAQTL ARPADFDFLH HIGESYATLR RYAPQFLGVL KLRAAPAAKG VLDAIDMLRG MNSDSARKVP ADAPTAFIKP RWAKLVLTDD GIDRRYYELC ALSELKNALR SGDVWVQGSR QFKDFDEYLV PVEKFATLKL ASELPLAVAT DCDQYLHDRL ELLEAQLATV NRMAAANDLP DAIITTASGL KITPLDAAVP DAAQAMIDQT AMLLPHLKIT ELLMEVDEWT GFTRHFTHLK TSDTAKDKTL LLTTILADAI NLGLTKMAES CPGTTYAKLS WLQAWHIRDE TYSTALAELV NAQFRQPFAG NWGDGTTSSS DGQNFRTGSK AESTGHINPK YGSSPGRTFY THISDQYAPF SAKVVNVGIR DSTYVLDGLL YHESDLRIEE HYTDTAGFTD HVFGLMHLLG FRFAPRIRDL GETKLFIPKG DAAYDALKPM ISSDRLNIKQ IRAHWDEILR LATSIKQGTV TASLMLRKLG SYPRQNGLAV ALRELGRIER TLFILDWLQS VELRRRVHAG LNKGEARNAL ARAVFFYRLG EIRDRSFEQQ RYRASGLNLV TAAIVLWNTV YLERATSALR GNGTALDDTL LQYLSPLGWE HINLTGDYLW RSSAKVGAGK FRPLRPLPPA |
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