Mobile element type:
Transposon
Name:
Tn163
Synonyms:
Accession:
Tn163-L14931
Family:
Tn3
Group:
Tn4430
First isolate:
Yes
Partial:
ND
Evidence of transposition:
ND
Host Organism:
R. leguminosarum bv.viciae strain E163N
Date of Isolation:
1994
Country:
Germany
Molecular Source:
plasmid pRleE163Na
| Name | Coordinates | Direction | Length | Sequence |
|---|---|---|---|---|
| IRL | 1-38 | + | 38 | GGCCCCCGAA CATTAAAGGG GCACAGATAT ACGGGATG |
| IRR | 4568-4605 | - | 38 | GGCCCCTGAA CATTAAAGGG GCGCAGATAT ACGGTAGG |
| Name | Associated TE | Coordinates | Orientation | Length | Sequence |
|---|---|---|---|---|---|
| res | Tn163 | 1524-1585 | + | 62 | AACCCGTCTT GCAATGTAAG CTCTATGAAC GGTTATCTCC GATCTTTTTG TGACGAACGG AG |
ORF Summary
| Name | Associated TE | Coordinates | Orientation | Class | Subclass |
|---|---|---|---|---|---|
| aminoglycoside phosphotransferase | Tn163 | 53-823 | - | Passenger Gene | Antibiotic Resistance |
| tnpR | Tn163 | 820-1461 | - | Accessory Gene | Resolvase |
| tnpA | Tn163 | 1591-4563 | + | Transposase |
ORF Details
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | aminoglycoside phosphotransferase | Aminoglycoside phosphotransferase | Tn163 | 256 | 53-823 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antibiotic Resistance | ||||||||||
| Sequence Family: | APH (Pfam:PF01636) | ||||||||||
| Target: | aminoglycoside antibiotic (ARO:0000016) | ||||||||||
| Comment: | no match in CARD | ||||||||||
| Protein Sequence: | MKELEGGRAG QIWRDGNTVR RPSGPWTPTV HRFLRHLQNS GFSAAPIPVE LTQHQETVTY VKGRVCEHLA DRLVGSERML RSAGELLRKF HSASRGFLDV DREIQMWMLP PQEPREIICH GDFAPYNVAT EGDAAVGIID FDTAHPAPAL WDLAYAVYRW APLSDYSNHG VVFDVREQLH RASIFCASYG ATADEKRRLP EAICGRLQAL VDFMHSRAET GDSTFVEDVE AGDAAIYLRD IEYVQKNRDR LLSALG |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpR | TnpR | Tn163 | 213 | 820-1461 | - |
|---|
| Class: | Accessory Gene | ||||||||||
| Subclass: | Resolvase | ||||||||||
| Function: | resolvase | ||||||||||
| Sequence Family: | Serine Site-Specific Recombinase | ||||||||||
| Transposase Chemistry: | Serine | ||||||||||
| Protein Sequence: | MTALIGYARV SKADGSQVHD LQRDALIKAG VEVDNVYEDS ASGKQDDRPG LAACLKLRAG DTLAVWKLDR LGRDLRHLVN LVDDLTKRNI GLKVLAGEGA SIDTSTANGR LVFGIFAALA EFERALIIER TKAGLAAARA RGRNGGAPFK MTPAKLRLAQ AMMGKPETKV ADLCAELGIT RQTLYRFVGP KGELRGDGER LLGRKKITLG VRK |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpA | TnpA | Tn163 | 990 | 1591-4563 | + |
|---|
| Class: | Transposase | ||||||||||
| Function: | transposase | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Protein Sequence: | MPRRMILTDA ERQNFLALPT DDDTLIRHWS LDDDDRRLIE TRRHDDTRLG LALQLCALRY PGRLIQRGEV IPSVALSFLA EQLGIDPEAL STFARRAATR YEQLAILRQH YGFTELSHPL RADLLAFARG IAIAATKDKF VVATLADEMR RRRIVIPGIT VLERLAGQAC TEAEEALYAD VAGRLAPDLI IRMEALLGAG RRAKQSGISW LREPPGKAGV SAMRGLIDRL EAVRHAGIPN DVLQAIPAHR VRRMAQEGRR LTAQNFEQMR PGRRHATLAA FLLEMEIALT DAAIGMFETL IGKSFRQAEA TRDKQLLESA SSATSALDFF ANFGEAITAQ RESGLSLDEA VTTVTSWEEL IQATASAKAV KRPRSEDDLI TYLSAQYVRI RRFVTPFLAS FTFEGNRRNA HFIEAIVRME TAWRKGSRSA EQPWVRKALL LANARWRKEI VGADGAIDRK MLELFLIVEL KNRIAAGDIW IRGSRAYRAL DEHMISQQTF AVIKAEARIP VAIPIDVETY LTQKAEALDQ KLREAARRLE AGRGDTRIGA KGLRVPAVRT TETEAATAFA RRVATTMPPV RLTDLVADID RMTGFSSLFE HLQTGRPPSN LRVFYAALIA EATNLGFSKM AHACPGITRR QLQQMAIWHF REETFTLALA KLVEAQHSTP FSTMFGSNTV SSSDGQHIHL GDGGEVAGSV NGRYGNNPII KLYTAISGGY APFHTKIIAA TASEAVHVLD ALLETDAGLH IIRHHVDGGG VSDLVFALCH ALGFAFVPRI PDLDGRCLYG FEPAKQYGIL QNVMGDRIDA DLIRANWDDI LRLMTSLRTR TVSASLMLKR LSATTRQSGL AQALRQMGRI ERTIFTLDWI NDEQLRKTTT AELNKGESRN SLVRAVNLHR LGRFRDRSQE NLSIRASALN LIVSAIIHWN TIYTGRIVDA LRNAGQAIPD HLLSSLSPLT WEHVNLTGDY LWEEKPALDE NGFRAIPFVL |
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1. Ulrich A, Puhler A.
The new class II transposon Tn163 is plasmid-borne in two unrelated Rhizobium leguminosarum biovar viciae strains.. Mol Gen Genet. 1994 Mar;242(5):505-16. doi: 10.1007/BF00285274. PubMed ID:
8121409
.