Mobile element type:
Transposon
Name:
Tn1546.2
Synonyms:
Accession:
Tn1546.2-AB247327
Family:
Tn3
Group:
Tn4430
First isolate:
Yes
Partial:
ND
Evidence of transposition:
ND
Host Organism:
Enterococcus faecalis KV1
Date of Isolation:
2006
Country:
Korea
Molecular Source:
plasmid pSL1
| Name | Coordinates | Direction | Length | Sequence |
|---|---|---|---|---|
| IRL | 1-38 | + | 38 | GGGGTACCGT CAGGAAAATG CGGATTTACA ACGTTAAG |
| IRR | 11631-11668 | - | 38 | GGGGTAGCGT CAGGAAAATG CGGATTTACA ACGCTAAG |
ORF Summary
| Name | Associated TE | Coordinates | Orientation | Class | Subclass |
|---|---|---|---|---|---|
| vanZA (ARO:3002962) | Tn1546.2 | 251-736 | - | Passenger Gene | Antibiotic Resistance |
| vanYA (ARO:3002955) | Tn1546.2 | 889-1800 | - | Passenger Gene | Antibiotic Resistance |
| tnp | IS1216E | 1902-2588 | + | Transposase | |
| vanXA (ARO:3002949) | Tn1546.2 | 3045-3653 | - | Passenger Gene | Antibiotic Resistance |
| vanA (ARO:3000010) | Tn1546.2 | 3659-4690 | - | Passenger Gene | Antibiotic Resistance |
| vanHA (ARO:3002942) | Tn1546.2 | 4683-5651 | - | Passenger Gene | Antibiotic Resistance |
| vanSA (ARO:3002931) | Tn1546.2 | 5866-6960 | - | Passenger Gene | Antibiotic Resistance |
| vanRA (ARO:3002919) | Tn1546.2 | 6998-7693 | - | Passenger Gene | Antibiotic Resistance |
| tnpR | Tn1546.2 | 7907-8485 | - | Accessory Gene | Resolvase |
| tnpA | Tn1546.2 | 8628-11594 | + | Transposase |
ORF Details
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | vanZA (ARO:3002962) | VanZA | Tn1546.2 | 161 | 251-736 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antibiotic Resistance | ||||||||||
| Function: | antibiotic target alteration (ARO:0001001) | ||||||||||
| Sequence Family: | vanZ (ARO:3000116)||glycopeptide resistance gene cluster (ARO:3000234) | ||||||||||
| Target: | glycopeptide antibiotic (ARO:3000081)||vancomycin (ARO:0000028) | ||||||||||
| Comment: | perfect match to reference sequence for ARO:3002962||Synonym: vanZ | ||||||||||
| Protein Sequence: | MGKILSRGLL ALYLVTLIWL VLFKLQYNIL SVFNYHQRSL NLTPFTATGN FREMIDNVII FIPFGLLLNV NFKEIGFLPK FAFVLVLSLT FEIIQFIFAI GATDITDVIT NTVGGFLGLK LYGLSNKHMN QKKLDRVIIF VGILLLVLLL VYRTHLRINY V |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | vanYA (ARO:3002955) | VanYA | Tn1546.2 | 303 | 889-1800 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antibiotic Resistance | ||||||||||
| Function: | antibiotic target alteration (ARO:0001001) | ||||||||||
| Sequence Family: | vanY (ARO:3000077)||glycopeptide resistance gene cluster (ARO:3000234) | ||||||||||
| Transposase Chemistry: | D-Ala-D-Ala carboxypeptidase | ||||||||||
| Target: | glycopeptide antibiotic (ARO:3000081)||vancomycin (ARO:0000028) | ||||||||||
| Comment: | perfect match to reference sequence for ARO:3002955||D-Ala-D-Ala carboxypeptidase||Synonym: vanY | ||||||||||
| Protein Sequence: | MKKLFFLLLL LFLIYLGYDY VNEALFSQEK VEFQNYDQNP KEHLENSGTS ENTQEKTITE EQVYQGNLLL INSKYPVRQE SVKSDIVNLS KHDELINGYG LLDSNIYMSK EIAQKFSEMV NDAVKGGVSH FIINSGYRDF DEQSVLYQEM GAEYALPAGY SEHNSGLSLD VGSSLTKMER APEGKWIEEN AWKYGFILRY PEDKTELTGI QYEPWHIRYV GLPHSAIMKE KNFVLEEYMD YLKEEKTISV SVNGEKYEIF YYPVTKNTTI HVPTNLRYEI SGNNIDGVIV TVFPGSTHTN SRR |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnp | Tnp | IS1216E | 228 | 1902-2588 | + |
|---|
| Class: | Transposase | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Protein Sequence: | MTMNHFKGKQ FQQDVIIVAV GYYLRYNLSY REVQEILYDR GINVSHTTIY RWVQEYGKLL YQIWKKKNKK SFYSWKMDET YIKIKGKWHY LYRAIDADGL TLDIWLRKKR DTQAAYAFLK RLVKQFDEPK VVVTDKAPSI TSAFKKLKEY GFYQGTEHRT IKYLNNLIEQ DHRPVKRRNK FYRSLRTAST TIKGMEAIRG LYKKTRKEGT LFGFSVCTEI KVLLGIPA |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | vanXA (ARO:3002949) | VanXA | Tn1546.2 | 202 | 3045-3653 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antibiotic Resistance | ||||||||||
| Function: | antibiotic target alteration (ARO:0001001) | ||||||||||
| Sequence Family: | vanX (ARO:3000011)||glycopeptide resistance gene cluster (ARO:3000234) | ||||||||||
| Transposase Chemistry: | D-Ala-D-Ala dipeptidase | ||||||||||
| Target: | glycopeptide antibiotic (ARO:3000081)||vancomycin (ARO:0000028) | ||||||||||
| Comment: | perfect match to reference sequence for ARO:3002949||Synonym: vanX | ||||||||||
| Protein Sequence: | MEIGFTFLDE IVHGVRWDAK YATWDNFTGK PVDGYEVNRI VGTYELAESL LKAKELAATQ GYGLLLWDGY RPKRAVNCFM QWAAQPENNL TKESYYPNID RTEMISKGYV ASKSSHSRGS AIDLTLYRLD TGELVPMGSR FDFMDERSHH AANGISCNEA QNRRRLRSIM ENSGFEAYSL EWWHYVLRDE PYPNSYFDFP VK |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | vanA (ARO:3000010) | VanA | Tn1546.2 | 343 | 3659-4690 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antibiotic Resistance | ||||||||||
| Function: | antibiotic target alteration (ARO:0001001) | ||||||||||
| Sequence Family: | glycopeptide resistance gene cluster (ARO:3000234)||van ligase (ARO:3002906) | ||||||||||
| Transposase Chemistry: | D-alanine--(R)-lactate ligase | ||||||||||
| Target: | glycopeptide antibiotic (ARO:3000081)||vancomycin (ARO:0000028) | ||||||||||
| Comment: | perfect match to reference sequence for ARO:3000010 | ||||||||||
| Protein Sequence: | MNRIKVAILF GGCSEEHDVS VKSAIEIAAN INKEKYEPLY IGITKSGVWK MCEKPCAEWE NDNCYSAVLS PDKKMHGLLV KKNHEYEINH VDVAFSALHG KSGEDGSIQG LFELSGIPFV GCDIQSSAIC MDKSLTYIVA KNAGIATPAF WVINKDDRPV AATFTYPVFV KPARSGSSFG VKKVNSADEL DYAIESARQY DSKILIEQAV SGCEVGCAVL GNSAALVVGE VDQIRLQYGI FRIHQEVEPE KGSENAVITV PADLSAEERG RIQETAKKIY KALGCRGLAR VDMFLQDNGR IVLNEVNTLP GFTSYSRYPR MMAAAGIALP ELIDRLIVLA LKG |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | vanHA (ARO:3002942) | VanHA | Tn1546.2 | 322 | 4683-5651 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antibiotic Resistance | ||||||||||
| Function: | antibiotic target alteration (ARO:0001001) | ||||||||||
| Sequence Family: | glycopeptide resistance gene cluster (ARO:3000234)||vanH (ARO:3000006) | ||||||||||
| Transposase Chemistry: | D-lactate dehydrogenase VanH-A | ||||||||||
| Target: | glycopeptide antibiotic (ARO:3000081)||vancomycin (ARO:0000028) | ||||||||||
| Comment: | perfect match to reference sequence for ARO:3002942||Synonym: vanH | ||||||||||
| Protein Sequence: | MNNIGITVYG CEQDEADAFH ALSPRFGVMA TIINANVSES NAKSAPFNQC ISVGHKSEIS ASILLALKRA GVKYISTRSI GCNHIDTTAA KRMGITVDNV AYSPDSVADY TMMLILMAVR NVKSIVRSVE KHDFRLDSDR GKVLSDMTVG VVGTGQIGKA VIERLRGFGC KVLAYSRSRS IEVNYVPFDE LLQNSDIVTL HVPLNTDTHY IISHEQIQRM KQGAFLINTG RGPLVDTYEL VKALENGKLG GAALDVLEGE EEFFYSDCTQ KPIDNQFLLK LQRMPNVIIT PHTAYYTEQA LRDTVEKTIK NCLDFERRQE HE |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | vanSA (ARO:3002931) | VanSA | Tn1546.2 | 364 | 5866-6960 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antibiotic Resistance | ||||||||||
| Function: | antibiotic target alteration (ARO:0001001) | ||||||||||
| Sequence Family: | glycopeptide resistance gene cluster (ARO:3000234)||vanS (ARO:3000071) | ||||||||||
| Transposase Chemistry: | VanA-type vancomycin resistance histidine kinase sensor | ||||||||||
| Target: | glycopeptide antibiotic (ARO:3000081)||vancomycin (ARO:0000028) | ||||||||||
| Comment: | strict match to reference sequence for ARO:3002931 (bitscore: 743)||Missing first 20 amino acids relative to vanSA (ARO:3002931)||Synonym: vanS | ||||||||||
| Protein Sequence: | MYIVAIVVVA IVFVLYIRSM IRGKLGDWIL SILENKYDLN HLDAMKLYQY SIRNNIDIFI YVAIVISILI LCRVMLSKFA KYFDEINTGI DVLIQNEDKQ IELSAEMDVM EQKLNTLKRT LEKREQDAKL AEQRKNDVVM YLAHDIKTPL TSIIGYLSLL DEAPDMPVDQ KAKYVHITLD KAYRLEQLID EFFEITRYNL QTITLTKTHI DLYYMLVQMT DEFYPQLSAH GKQAVIHAPE DLTVSGDPDK LARVFNNILK NAAAYSEDNS IIDITAGLSG DVVSIEFKNT GSIPKDKLAA IFEKFYRLDN ARSSDTGGAG LGLAIAKEII VQHGGQIYAE SNDNYTTFRV ELPAMPDLVD KRRS |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | vanRA (ARO:3002919) | VanRA | Tn1546.2 | 231 | 6998-7693 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antibiotic Resistance | ||||||||||
| Function: | antibiotic target alteration (ARO:0001001) | ||||||||||
| Sequence Family: | glycopeptide resistance gene cluster (ARO:3000234)||vanR (ARO:3000574) | ||||||||||
| Transposase Chemistry: | VanA-type vancomycin resistance DNA-binding response regulator | ||||||||||
| Target: | glycopeptide antibiotic (ARO:3000081)||vancomycin (ARO:0000028) | ||||||||||
| Comment: | perfect match to reference sequence for ARO:3002919||Synonym: vanR | ||||||||||
| Protein Sequence: | MSDKILIVDD EHEIADLVEL YLKNENYTVF KYYTAKEALE CIDKSEIDLA ILDIMLPGTS GLTICQKIRD KHTYPIIMLT GKDTEVDKIT GLTIGADDYI TKPFRPLELI ARVKAQLRRY KKFSGVKEQN ENVIVHSGLV INVNTHECYL NEKQLSLTPT EFSILRILCE NKGNVVSSEL LFHEIWGDEY FSKSNNTITV HIRHLREKMN DTIDNPKYIK TVWGVGYKIE K |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpR | TnpR | Tn1546.2 | 192 | 7907-8485 | - |
|---|
| Class: | Accessory Gene | ||||||||||
| Subclass: | Resolvase | ||||||||||
| Function: | resolvase; serine site-specific recombinase | ||||||||||
| Sequence Family: | Serine Site-Specific Recombinase | ||||||||||
| Transposase Chemistry: | Serine | ||||||||||
| Protein Sequence: | MLRKIGYIRV SSTNQNPSRQ FQQLNEIGMD IIYEEKVSGA TKDREQLQKV LDDLQEDDII YVTDLTRITR STQDLFELID NIRDKKASLK SLKDTWLDLS EDNPYSQFLI TVMAGVNQLE RDLIRMRQRE GIELAKKEGK FKGRLKKYHK NHAGMNYAVK LYKEGNMTVN QICEITNVSR ASLYRKLSEV NN |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpA | TnpA | Tn1546.2 | 988 | 8628-11594 | + |
|---|
| Class: | Transposase | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Protein Sequence: | MKIARGRELL TPEQRQAFMQ IPEDEWILGT YFTFSKRDLE IVNKRRREEN RLGFAVQLAV LRYPGWPYTH IKSIPDSVIQ YISKQIGVSP SSLDHYPQRE NTLWDHLKEI RSEYDFVTFT LSEYRMTFKY LHQLALENGD AIHLLHECID FLRKNKIILP AITTLERMVW EARAMAEKKL FNTVSKSLTN EQKEKLEGII TSQHPSESNK TILGWLKEPP GHPSPETFLK IIERLEYIRG MDLETVQISH LHRNRLLQLS RLGSRYEPYA FRDFQENKRY SILTIYLLQL TQELTDKAFE IHDRQILSLL SKGRKAQEEI QKQNGKKLNE KVIHFTNIGQ ALIKAREEKL DVFKVLESVI EWNTFVSSVE EAQELARPAD YDYLDLLQKR FYSLRKYTPT LLRVLEFHST KANEPLLQAV EIIRGMNESG KRKVPDDSPV DFISKRWKRH LYEDDGTTIN RHYYEMAVLT ELREHVRAGD VSIVGSRQYR DFEEYLFSED TWNQSKGNTR LSVSLSFEDY ITERTSSFNE RLKWLAANSN KLDGVSLEKG KLSLARLEKD VPEEAKKFSA SLYQMLPRIK LTDLLMDVAH ITGFHEQFTH ASNNRKPDKE ETIIIMAALL GMGMNIGLSK MAEATPGLTY KQLANVSQWR MYEDAMNKAQ AILVNFHHKL QLPFYWGDGT TSSSDGMRMQ LGVSSLHADA NPHYGTGKGA TIYRFTSDQF SSYYTKIIHT NSRDAIHVLD GLLHHETDLN IEEHYTDTAG YTDQIFGLTH LLGFKFAPRI RDLSDSKLFT IDKASEYPKL EAILRGQINT KVIKENYEDV LRLAHSIREG TVSASLIMGK LGSYSRQNSL ATALREMGRI EKTIFILNYI SDESLRRKIQ RGLNKGEAMN GLARAIFFGK QGELRERTIQ HQLQRASALN IIINAISIWN TLHLTTAVEY KKRTGSFNED LLHHMSPLGW EHINLLGEYH FNSEKVVSLN SLRPLKLS |
||||||||||
| TnCentral Accession | TE Name | Type | Coordinates | Strand | Length |
|---|---|---|---|---|---|
| IS1216E-U49512 | IS1216E | insertion sequence | 1834-2642 | + | 809 |
| Name | Associated TE | Coordinates | Direction | Length | Sequence |
|---|---|---|---|---|---|
| IRL | IS1216E | 1834-1857 | + | 24 | GGTTCTGTTG CAAAGTTTTA AATC |
| IRR | IS1216E | 2620-2642 | - | 23 | GGTTCTGTTG CAAAGTTTTA AAT |
1. Lim SK, Tanimoto K, Tomita H, Ike Y.
Pheromone-responsive conjugative vancomycin resistance plasmids in Enterococcus faecalis isolates from humans and chicken feces.. Appl Environ Microbiol. 2006 Oct;72(10):6544-53. doi: 10.1128/AEM.00749-06. PubMed ID:
17021204
.