Mobile element type:
Transposon
Name:
Tn1546.1_p
Synonyms:
Accession:
Tn1546.1_p-KR349520.1
Family:
Tn3
Group:
Tn4430
First isolate:
Yes
Partial:
Yes
Evidence of transposition:
ND
Host Organism:
Enterococcus faecium VREfm 320/07
Date of Isolation:
2015
Country:
Brazil
| Name | Coordinates | Direction | Length | Sequence |
|---|---|---|---|---|
| IRL | 9-46 | + | 38 | GGGGTACCGT CAGGAAAATG CGGATTTACA ACGTTAAG |
ORF Summary
| Name | Associated TE | Coordinates | Orientation | Class | Subclass |
|---|---|---|---|---|---|
| vanZA (ARO:3002962) | Tn1546.1_p | 259-744 | - | Passenger Gene | Antibiotic Resistance |
| vanYA (ARO:3002955) | Tn1546.1_p | 897-1808 | - | Passenger Gene | Antibiotic Resistance |
| tnp | IS1216E | 2203-2889 | + | Transposase | |
| vanXA (ARO:3002949) | Tn1546.1_p | 2969-3577 | - | Passenger Gene | Antibiotic Resistance |
| vanA (ARO:3000010) | Tn1546.1_p | 3583-4614 | - | Passenger Gene | Antibiotic Resistance |
| vanHA (ARO:3002942) | Tn1546.1_p | 4607-5575 | - | Passenger Gene | Antibiotic Resistance |
| tnp | IS1251 | 5907-7199 | + | Transposase | |
| vanSA (ARO:3002931) | Tn1546.1_p | 7297-8391 | - | Passenger Gene | Antibiotic Resistance |
| vanRA (ARO:3002919) | Tn1546.1_p | 8429-9124 | - | Passenger Gene | Antibiotic Resistance |
| tnpR | Tn1546.1_p | 9338-9916 | - | Accessory Gene | Resolvase |
| tnpA | Tn1546.1_p | 10059-12218 | + | Transposase |
ORF Details
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | vanZA (ARO:3002962) | VanZA | Tn1546.1_p | 161 | 259-744 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antibiotic Resistance | ||||||||||
| Function: | antibiotic target alteration (ARO:0001001) | ||||||||||
| Sequence Family: | vanZ (ARO:3000116)||glycopeptide resistance gene cluster (ARO:3000234) | ||||||||||
| Target: | glycopeptide antibiotic (ARO:3000081)||vancomycin (ARO:0000028) | ||||||||||
| Comment: | perfect match to reference sequence for ARO:3002962||Synonym: vanZ | ||||||||||
| Protein Sequence: | MGKILSRGLL ALYLVTLIWL VLFKLQYNIL SVFNYHQRSL NLTPFTATGN FREMIDNVII FIPFGLLLNV NFKEIGFLPK FAFVLVLSLT FEIIQFIFAI GATDITDVIT NTVGGFLGLK LYGLSNKHMN QKKLDRVIIF VGILLLVLLL VYRTHLRINY V |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | vanYA (ARO:3002955) | VanYA | Tn1546.1_p | 303 | 897-1808 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antibiotic Resistance | ||||||||||
| Function: | antibiotic target alteration (ARO:0001001) | ||||||||||
| Sequence Family: | vanY (ARO:3000077)||glycopeptide resistance gene cluster (ARO:3000234) | ||||||||||
| Transposase Chemistry: | D-Ala-D-Ala carboxypeptidase | ||||||||||
| Target: | glycopeptide antibiotic (ARO:3000081)||vancomycin (ARO:0000028) | ||||||||||
| Comment: | perfect match to reference sequence for ARO:3002955||D-Ala-D-Ala carboxypeptidase||Synonym: vanY | ||||||||||
| Protein Sequence: | MKKLFFLLLL LFLIYLGYDY VNEALFSQEK VEFQNYDQNP KEHLENSGTS ENTQEKTITE EQVYQGNLLL INSKYPVRQE SVKSDIVNLS KHDELINGYG LLDSNIYMSK EIAQKFSEMV NDAVKGGVSH FIINSGYRDF DEQSVLYQEM GAEYALPAGY SEHNSGLSLD VGSSLTKMER APEGKWIEEN AWKYGFILRY PEDKTELTGI QYEPWHIRYV GLPHSAIMKE KNFVLEEYMD YLKEEKTISV SVNGEKYEIF YYPVTKNTTI HVPTNLRYEI SGNNIDGVIV TVFPGSTHTN SRR |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnp | Tnp | IS1216E | 228 | 2203-2889 | + |
|---|
| Class: | Transposase | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Protein Sequence: | MTMNHFKGKQ FQQDVIIVAV GYYLRYNLSY REVQEILYDR GINVSHTTIY RWVQEYGKLL YQIWKKKNKK SFYSWKMDET YIKIKGKWHY LYRAIDADGL TLDIWLRKKR DTQAAYAFLK RLVKQFDEPK VVVTDKAPSI TSAFKKLKEY GFYQGTEHRT IKYLNNLIEQ DHRPVKRRNK FYRSLRTAST TIKGMEAIRG LYKKTRKEGT LFGFSVCTEI KVLLGIPA |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | vanXA (ARO:3002949) | VanXA | Tn1546.1_p | 202 | 2969-3577 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antibiotic Resistance | ||||||||||
| Function: | antibiotic target alteration (ARO:0001001) | ||||||||||
| Sequence Family: | vanX (ARO:3000011)||glycopeptide resistance gene cluster (ARO:3000234) | ||||||||||
| Transposase Chemistry: | D-Ala-D-Ala dipeptidase | ||||||||||
| Target: | glycopeptide antibiotic (ARO:3000081)||vancomycin (ARO:0000028) | ||||||||||
| Comment: | perfect match to reference sequence for ARO:3002949||Synonym: vanX | ||||||||||
| Protein Sequence: | MEIGFTFLDE IVHGVRWDAK YATWDNFTGK PVDGYEVNRI VGTYELAESL LKAKELAATQ GYGLLLWDGY RPNRAVNCFM QWAAQPENNL TKESYYPNID RTEMISKGYV ASKSSHSRGS AIDLTLYRLD TGELVPMGSR FDFMDERSHH AANGISCNEA QNRRRLRSIM ENSGFEAYSL EWWHYVLRDE PYPNSYFDFP VK |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | vanA (ARO:3000010) | VanA | Tn1546.1_p | 343 | 3583-4614 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antibiotic Resistance | ||||||||||
| Function: | antibiotic target alteration (ARO:0001001) | ||||||||||
| Sequence Family: | glycopeptide resistance gene cluster (ARO:3000234)||van ligase (ARO:3002906) | ||||||||||
| Transposase Chemistry: | D-alanine--(R)-lactate ligase | ||||||||||
| Target: | glycopeptide antibiotic (ARO:3000081)||vancomycin (ARO:0000028) | ||||||||||
| Comment: | strict match to reference sequence for ARO:3000010 (bitscore: 705) | ||||||||||
| Protein Sequence: | MNRIKVAILF GGCSEEHDVS VKSAIEIAAN INKEKYEPLY IGITKSGVWK MCEKPCAEWE NDNCYSAVLS PDKKMHGLLV KKNHEYEINH VDVAFSALHG KSGEDGSIQG LFELSGIPFV GCDIQSSAIC MDKSLTYIVA KNAGIATPAF WVINKDDRPV AATFTYPVFV KPARSGSSFG VKKVNSADEL DYAIESARQY DSKILIEQAV SGCEVGCAVL GNSAALAVGE VDQIRLQYGI FRIHQEVEPE KGSENAVITV PADLSAEERG RIQETAKKIY KALGCRGLAR VDMFLQDNGR IVLNEVNTLP GFTSYSRYPR MMAAAGIALP ELIDRLIVLA LKG |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | vanHA (ARO:3002942) | VanHA | Tn1546.1_p | 322 | 4607-5575 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antibiotic Resistance | ||||||||||
| Function: | antibiotic target alteration (ARO:0001001) | ||||||||||
| Sequence Family: | glycopeptide resistance gene cluster (ARO:3000234)||vanH (ARO:3000006) | ||||||||||
| Transposase Chemistry: | D-lactate dehydrogenase VanH-A | ||||||||||
| Target: | glycopeptide antibiotic (ARO:3000081)||vancomycin (ARO:0000028) | ||||||||||
| Comment: | perfect match to reference sequence for ARO:3002942||Synonym: vanH | ||||||||||
| Protein Sequence: | MNNIGITVYG CEQDEADAFH ALSPRFGVMA TIINANVSES NAKSAPFNQC ISVGHKSEIS ASILLALKRA GVKYISTRSI GCNHIDTTAA KRMGITVDNV AYSPDSVADY TMMLILMAVR NVKSIVRSVE KHDFRLDSDR GKVLSDMTVG VVGTGQIGKA VIERLRGFGC KVLAYSRSRS IEVNYVPFDE LLQNSDIVTL HVPLNTDTHY IISHEQIQRM KQGAFLINTG RGPLVDTYEL VKALENGKLG GAALDVLEGE EEFFYSDCTQ KPIDNQFLLK LQRMPNVIIT PHTAYYTEQA LRDTVEKTIK NCLDFERRQE HE |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnp | Tnp | IS1251 | 430 | 5907-7199 | + |
|---|
| Class: | Transposase | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Protein Sequence: | MSYTHLIKET LDILDLSVTF NENCLTKEKY KGQICHIYRG NLIYTAQECI HCKHQIASDI VRWGTTTVRL LMNDVSEYRT YLELKKQRFK CKACQRTFVA DTSVAKKHCF ISEKVRWSVI ARLKKNTSMT EIARQKNIST SSVYRVMKRF YRPLNPFKQT LPKVLCFDEF KSVRSVASAM SFIMMDGQSH ALLDIVENRR LPYLERYFSR FSLATREAVQ LIVIDMYAPY VSLVKKLFPK AQLIIDRFHI VQHIGRTFRN HRIKETNQLL KSKEQKHYQL GKQLKRYWKL LQKDERKLDY TRRLWRPGFK AHLTETDIVD RLLKGSPALR VGYQLYQDFL YAVKERDYVS FEELLTNNIM LPEGYQTTLR TFQKFLPQIK NALQQSYSNG PLECLNNHIK VLKRNAYGFR SFYNFKLRIM IRHGNALIFN |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | vanSA (ARO:3002931) | VanSA | Tn1546.1_p | 364 | 7297-8391 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antibiotic Resistance | ||||||||||
| Function: | antibiotic target alteration (ARO:0001001) | ||||||||||
| Sequence Family: | glycopeptide resistance gene cluster (ARO:3000234)||vanS (ARO:3000071) | ||||||||||
| Transposase Chemistry: | VanA-type vancomycin resistance histidine kinase sensor | ||||||||||
| Target: | glycopeptide antibiotic (ARO:3000081)||vancomycin (ARO:0000028) | ||||||||||
| Comment: | strict match to reference sequence for ARO:3002931 (bitscore: 743)||Missing first 20 amino acids relative to vanSA (ARO:3002931)||Synonym: vanS | ||||||||||
| Protein Sequence: | MYIVAIVVVA IVFVLYIRSM IRGKLGDWIL SILENKYDLN HLDAMKLYQY SIRNNIDIFI YVAIVISILI LCRVMLSKFA KYFDEINTGI DVLIQNEDKQ IELSAEMDVM EQKLNTLKRT LEKREQDAKL AEQRKNDVVM YLAHDIKTPL TSIIGYLSLL DEAPDMPVDQ KAKYVHITLD KAYRLEQLID EFFEITRYNL QTITLTKTHI DLYYMLVQMT DEFYPQLSAH GKQAVIHAPE DLTVSGDPDK LARVFNNILK NAAAYSEDNS IIDITAGLSG DVVSIEFKNT GSIPKDKLAA IFEKFYRLDN ARSSDTGGAG LGLAIAKEII VQHGGQIYAE SNDNYTTFRV ELPAMPDLVD KRRS |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | vanRA (ARO:3002919) | VanRA | Tn1546.1_p | 231 | 8429-9124 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antibiotic Resistance | ||||||||||
| Function: | antibiotic target alteration (ARO:0001001) | ||||||||||
| Sequence Family: | glycopeptide resistance gene cluster (ARO:3000234)||vanR (ARO:3000574) | ||||||||||
| Transposase Chemistry: | VanA-type vancomycin resistance DNA-binding response regulator | ||||||||||
| Target: | glycopeptide antibiotic (ARO:3000081)||vancomycin (ARO:0000028) | ||||||||||
| Comment: | perfect match to reference sequence for ARO:3002919||Synonym: vanR | ||||||||||
| Protein Sequence: | MSDKILIVDD EHEIADLVEL YLKNENYTVF KYYTAKEALE CIDKSEIDLA ILDIMLPGTS GLTICQKIRD KHTYPIIMLT GKDTEVDKIT GLTIGADDYI TKPFRPLELI ARVKAQLRRY KKFSGVKEQN ENVIVHSGLV INVNTHECYL NEKQLSLTPT EFSILRILCE NKGNVVSSEL LFHEIWGDEY FSKSNNTITV HIRHLREKMN DTIDNPKYIK TVWGVGYKIE K |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpR | TnpR | Tn1546.1_p | 192 | 9338-9916 | - |
|---|
| Class: | Accessory Gene | ||||||||||
| Subclass: | Resolvase | ||||||||||
| Function: | resolvase; serine site-specific recombinase | ||||||||||
| Sequence Family: | Serine Site-Specific Recombinase | ||||||||||
| Transposase Chemistry: | Serine | ||||||||||
| Protein Sequence: | MLRKIGYIRV SSTNQNPSRQ FQQLNEIGMD IIYEEKVSGA TKDREQLQKV LDDLQEDDII YVTDLTRITR STQDLFELID NIRDKKASLK SLKDTWLDLS EDNPYSQFLI TVMAGVNQLE RDLIRMRQRE GIELAKKEGK FKGRLKKYHK NHAGMNYAVK LYKEGNMTVN QICEITNVSR ASLYRKLSEV NN |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnpA | TnpA | Tn1546.1_p | 719 | 10059-12218 | + |
|---|
| Class: | Transposase | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Protein Sequence: | MKIARGRELL TPEQRQAFMQ IPEDEWILGT YFTFSKRDLE IVNKRRREEN RLGFAVQLAV LRYPGWPYTH IKSIPDSVIQ YISKQIGVSP SSLDHYPQRE NTLWDHLKEI RSEYDFVTFT LSEYRMTFKY LHQLALENGD AIHLLHECID FLRKNKIILP AITTLERMVW EARAMAEKKL FNTVSKSLTN EQKEKLEGII TSQHPSESNK TILGWLKEPP GHPSPETFLK IIERLEYIRG MDLETVQISH LHRNRLLQLS RLGSRYEPYA FRDFQENKRY SILTIYLLQL TQELTDKAFE IHDRQILSLL SKGRKAQEEI QKQNGKKLNE KVIHFTNIGQ ALIKAREEKL DVFKVLESVI EWNTFVSSVE EAQELARPAD YDYLDLLQKR FYSLRKYTPT LLRVLEFHST KANEPLLQAV EIIRGMNESG KRKVPDDSPV DFISKRWKRH LYEDDGTTIN RHYYEMAVLT ELREHVRAGD VSIVGSRQYR DFEEYLFSED TWNQSKGNTR LSVSLSFEDY ITERTSSFNE RLKWLAANSN KLDGVSLEKG KLSLARLEKD VPEEAKKFSA SLYQMLPRIK LTDLLMDVAH ITGFHEQFTH ASNNRKPDKE ETIIIMAALL GMGMNIGLSK MAEATPGLTY KQLANVSQWR MYEDAMNKAQ AILVNFHHKL QLPFYWGDGT TSSSDGMRMQ LGVSSLHADA NPHYGTGKGA TIYRFTSGK |
||||||||||
| TnCentral Accession | TE Name | Type | Coordinates | Strand | Length |
|---|---|---|---|---|---|
| IS1216E-U49512 | IS1216E | insertion sequence | 2135-2943 | + | 809 |
| IS1251-KR349520.1 | IS1251 | insertion sequence | 5781-7279 | + | 1499 |
| Name | Associated TE | Coordinates | Direction | Length | Sequence |
|---|---|---|---|---|---|
| IRL | IS1216E | 2135-2158 | + | 24 | GGTTCTGTTG CAAAGTTTTA AATC |
| IRR | IS1216E | 2921-2943 | - | 23 | GGTTCTGTTG CAAAGTTTTA AAT |
| IRL | IS1251 | 5781-5804 | + | 24 | GGCTCTATAC TTTTTGATGT TGGT |
| IRR | IS1251 | 7256-7279 | - | 24 | GGCTCTTTGT CAAATGATGT TGGT |