Mobile element type:
Transposon
Name:
Tn10
Synonyms:
Accession:
Tn10-AF162223
Family:
compound
Group:
IS10
First isolate:
ND
Partial:
ND
Evidence of transposition:
ND
Host Organism:
Shigella flexneri
Date of Isolation:
1975
Country:
U.s.a and u.k.
Molecular Source:
plasmid R100
ORF Summary
Name | Associated TE | Coordinates | Orientation | Class | Subclass |
---|---|---|---|---|---|
tnp | IS10L | 108-1316 | + | Transposase | |
jemA | Tn10 | 1682-2887 | - | Passenger Gene | Other |
jemB | Tn10 | 3331-3651 | + | Passenger Gene | Other |
CPT07_26605 | Tn10 | 3644-4030 | + | Passenger Gene | Hypothetical |
jemC | Tn10 | 4038-4724 | + | Passenger Gene | Other |
tetR (ARO:3003479) | Tn10 | 4702-5328 | - | Passenger Gene | Antibiotic Resistance |
tet(B) (ARO:3000166) | Tn10 | 5407-6612 | + | Passenger Gene | Antibiotic Resistance |
tetC | Tn10 | 6725-7318 | - | Passenger Gene | Antibiotic Resistance |
tetD | Tn10 | 7406-7822 | + | Passenger Gene | Antibiotic Resistance |
tnp | IS10R | 7832-9040 | - | Transposase |
ORF Details
Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnp | Tnp | IS10L | 402 | 108-1316 | + |
---|
Class: | Transposase | ||||||||||
Function: | transposase | ||||||||||
Transposase Chemistry: | DDE | ||||||||||
Protein Sequence: | MCELDILHDS LYQFCPELHL KRLNSLTLAC HALLDCKTLT LTELGRNLPT KARTKHNIKR IDRLLGNRHL HKERLAVYRW HASFICSGNT MPIVLVDWSD IREQKRLMVL RASVALHGRS VTLYEKAFPL SEQCSKKAHD QFLADLASIL PSNTTPLIVS DAGFKVPWYK SVEKLGWYWL SRVRGKVQYA DLGAENWKPI SNLHDMSSSH SKTLGYKRLT KSNPISCQIL LYKSRSKGRK NQRSTRTHCH HPSPKIYSAS AKEPWILATN LPVEIRTPKQ LVNIYSKRMQ IEETFRDLKS PAYGLGLRHS RTSSSERFDI MLLIALMLQL TCWLAGVHAQ KQGWDKHFQA NTVRNRNVLS TVRLGMEVLR HSGYTITRED SLVAATLLTQ NLFTHGYVLG KL |
Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | jemA | JemA | Tn10 | 401 | 1682-2887 | - |
---|
Class: | Passenger Gene | ||||||||||
Subclass: | Other | ||||||||||
Function: | glutamate:sodium symporter activity (GO:0015501) | ||||||||||
Comment: | sodium-dependent glutamate permease | ||||||||||
Protein Sequence: | MILDASYTLL VACIALLIGM FVVKFTPFLQ KNHIPEAVVG GFIVAIVLLI IDKTSGYSFT FDASLQSLLM LTFFSSIGLS SDFSRLIKGG KPLVLLTIAV TILIAIQNTV GMSMAVMMNE SPFIGLIAGS ITLTGGHGNA GAWGPILADK YGVTGAVELA MACATLGLVL GGLVGGPVAR HLLKKVSIPK TTEQERDTIV EAFEQPSVKR KINANNVIET ISMLIICIVV GGYISALFKD TFLQLPTFVW CLFVGIIIRN TLTHVFKHEV FEPTVDVLGS VALSLFLAMA LMSLKFGQLA SMAGPVLIII AVQTVVMVLF ACFVTFKMMG KDYDAVVISA GHCGFGMGAT PTAIANMQTV TKAFGPSHKA FLVVPMVGAF IVDISNSILI KIFIEIGTYF T |
Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | jemB | JemB | Tn10 | 106 | 3331-3651 | + |
---|
Class: | Passenger Gene | ||||||||||
Subclass: | Other | ||||||||||
Sequence Family: | Antibiotic biosynthesis monooxygenase (Pfam:PF03992) | ||||||||||
Protein Sequence: | MIAVIFEVQI QPDQQTRYLT LAEELRPLLS HVAGFISIER FQSLATEGKM LSLSWWENEY AVLQWKNHVL HAKAQQEGRE SIFDFYKISI AHITREYSFK KDKDNV |
Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | CPT07_26605 | CPT07_26605 | Tn10 | 128 | 3644-4030 | + |
---|
Class: | Passenger Gene | ||||||||||
Subclass: | Hypothetical | ||||||||||
Comment: | Amino acid-binding protein | ||||||||||
Protein Sequence: | MFDVHVVLDN QIGQLALLGK TLGNKGIGLE GGGIFTVGDE CHAHFLVEQG KEAKIALEQA GLLVLAIRTP LIRKLKQEKP GELGEIARVL AENNINILVQ YSDHANQLIL ITDNDSMAAS VTLPWAIK |
Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | jemC | JemC | Tn10 | 228 | 4038-4724 | + |
---|
Class: | Passenger Gene | ||||||||||
Subclass: | Other | ||||||||||
Sequence Family: | HTH ArsR-type DNA-binding domain (InterPro:IPR001845) | ||||||||||
Comment: | N-terminal 100 amino acids similar to bacterial transcriptional repressors and metal-binding proteins (PMID:10781570) | ||||||||||
Protein Sequence: | MANLIRKEVT FESSIAAIGA AMSDISRVKI LSALMDGRAW TATELSSVAN ISASTASSHL SKLLDCQLIT VVAQGKHRYF RLAGKDIAEL MESMMGISLN HGVHAKVSTP VHLRKARTCY DHLAGEVAVK IYDSLCQQQW ITENGSMITL SGIQYFHEMG IDVPSKHSRK ICCACLDWSE RRFHLGGYVG AALFSLYESK GWLTRHLGYR EVTITEKGYA AFKTHFHI |
Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tetR (ARO:3003479) | TetR | Tn10 | 208 | 4702-5328 | - |
---|
Class: | Passenger Gene | ||||||||||
Subclass: | Antibiotic Resistance | ||||||||||
Function: | antibiotic target alteration (ARO:0001001)||antibiotic efflux (ARO:0010000) | ||||||||||
Sequence Family: | major facilitator superfamily (MFS) antibiotic efflux pump (ARO:0010002) | ||||||||||
Transposase Chemistry: | repressor of the tetracycline resistance element | ||||||||||
Target: | tetracycline antibiotic (ARO:3000050) | ||||||||||
Comment: | perfect match to reference sequence for ARO:3003479 | ||||||||||
Protein Sequence: | MMSRLDKSKV INSALELLNE VGIEGLTTRK LAQKLGVEQP TLYWHVKNKR ALLDALAIEM LDRHHTHFCP LEGESWQDFL RNNAKSFRCA LLSHRDGAKV HLGTRPTEKQ YETLENQLAF LCQQGFSLEN ALYALSAVGH FTLGCVLEDQ EHQVAKEERE TPTTDSMPPL LRQAIELFDH QGAEPAFLFG LELIICGLEK QLKCESGS |
Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tet(B) (ARO:3000166) | Tet(B) | Tn10 | 401 | 5407-6612 | + |
---|
Class: | Passenger Gene | ||||||||||
Subclass: | Antibiotic Resistance | ||||||||||
Function: | antibiotic efflux (ARO:0010000) | ||||||||||
Sequence Family: | major facilitator superfamily (MFS) antibiotic efflux pump (ARO:0010002) | ||||||||||
Transposase Chemistry: | antibiotic efflux (ARO:0010000) | ||||||||||
Target: | tetracycline antibiotic (ARO:3000050) | ||||||||||
Comment: | strict match to reference sequence for ARO:3000166 (bitscore:786)||Synonym: tetB | ||||||||||
Protein Sequence: | MNSSTKIALV ITLLDAMGIG LIMPVLPTLL REFIASEDIA NHFGVLLALY ALMQVIFAPW LGKMSDRFGR RPVLLLSLIG ASLDYLLLAF SSALWMLYLG RLLSGITGAT GAVAASVIAD TTSASQRVKW FGWLGASFGL GLIAGPIIGG FAGEISPHSP FFIAALLNIV TFLVVMFWFR ETKNTRDNTD TEVGVETQSN SVYITLFKTM PILLIIYFSA QLIGQIPATV WVLFTENRFG WNSMMVGFSL AGLGLLHSVF QAFVAGRIAT KWGEKTAVLL GFIADSSAFA FLAFISEGWL VFPVLILLAG GGIALPALQG VMSIQTKSHQ QGALQGLLVS LTNATGVIGP LLFAVIYNHS LPIWDGWIWI IGLAFYCIII LLSMTFMLTP QAQGSKQETS A |
Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tetC | TetC | Tn10 | 197 | 6725-7318 | - |
---|
Class: | Passenger Gene | ||||||||||
Subclass: | Antibiotic Resistance | ||||||||||
Function: | DNA binding (GO:0003677) | ||||||||||
Sequence Family: | Bacterial regulatory proteins, tetR family (Pfam:PF00440) | ||||||||||
Target: | tetracycline antibiotic (ARO:3000050) | ||||||||||
Comment: | no match inCARD | ||||||||||
Protein Sequence: | MENKNHQQEN FKSTYQSLVN SARILFVEKG YQAVSIDEIS GKALVTKGAF YHHFKNKKQL LSACYKQQLI MIDAYITTKT DLTNGWSALE SIFEHYLDYI IDNNKNLIPI QEVMPIIGWN ELEKISLEYI TGKVNAIVSK LIQENQLKAY DDDVLKNLLN GWFMHIAIHA KNLKELADKK GQFIAIYRGF LLSLKDK |
Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tetD | TetD | Tn10 | 138 | 7406-7822 | + |
---|
Class: | Passenger Gene | ||||||||||
Subclass: | Antibiotic Resistance | ||||||||||
Target: | tetracycline antibiotic (ARO:3000050) | ||||||||||
Comment: | no match in CARD | ||||||||||
Protein Sequence: | MYIEQHSRYQ NKANNIQLRY DDKQFHTTVI KDVLLWIEHN LDQSLLLDDV ANKAGYTKWY FQRLFKKVTG VTLASYIRAR RLTKAAVELR LTKKTILEIA LKYQFDSQQS FTRRFKYIFK VTPSYYRRNK LWELEAMH |
Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnp | Tnp | IS10R | 402 | 7832-9040 | - |
---|
Class: | Transposase | ||||||||||
Function: | transposase | ||||||||||
Transposase Chemistry: | DDE | ||||||||||
Protein Sequence: | MCELDILHDS LYQFCPELHL KRLNSLTLAC HALLDCKTLT LTELGRNLPT KARTKHNIKR IDRLLGNRHL HKERLAVYRW HASFICSGNT MPIVLVDWSD IREQKRLMVL RASVALHGRS VTLYEKAFPL SEQCSKKAHD QFLADLASIL PSNTTPLIVS DAGFKVPWYK SVEKLGWYWL SRVRGKVQYA DLGAENWKPI SNLHDMSSSH SKTLGYKRLT KSNPISCQIL LYKSRSKGRK NQRSTRTHCH HPSPKIYSAS AKEPWVLATN LPVEIRTPKQ LVNIYSKRMQ IEETFRDLKS PAYGLGLRHS RTSSSERFDI MLLIALMLQL TCWLAGVHAQ KQGWDKHFQA NTVRNRNVLS TVRLGMEVLR HSGYTITRED LLVAATLLAQ NLFTHGYALG KL |
TnCentral Accession | TE Name | Type | Coordinates | Strand | Length |
---|---|---|---|---|---|
IS10L-AF162223 | IS10L | insertion sequence | 1-1329 | + | 1329 |
IS10R-AF162223 | IS10R | insertion sequence | 7819-9147 | + | 1329 |
Name | Associated TE | Coordinates | Direction | Length | Sequence |
---|---|---|---|---|---|
IRL | IS10L | 1-17 | + | 17 | CTGATGAATC CCCTAAT |
IRR | IS10L | 1308-1329 | - | 22 | CTGAGAGATC CCCTCATAAT TT |
IRR | IS10R | 7819-7840 | + | 22 | CTGAGAGATC CCCTCATAAT TT |
IRL | IS10R | 9131-9147 | - | 17 | CTGATGAATC CCCTAAT |
1. Foster TJ, Howe TG, Richmond KM.
Translocation of the tetracycline resistance determinant from R100-1 to the Escherichia coli K-12 chromosome.. J Bacteriol. 1975 Dec;124(3):1153-8. doi: 10.1128/jb.124.3.1153-1158.1975. PubMed ID:
1104574
.
2. Kleckner N, Chan RK, Tye BK, Botstein D. Mutagenesis by insertion of a drug-resistance element carrying an inverted repetition.. J Mol Biol. 1975 Oct 5;97(4):561-75. doi: 10.1016/s0022-2836(75)80059-3. PubMed ID: 1102715 .
3. Foster TJ. Insertion of the tetracycline resistance translocation unit Tn10 in the lac operon of Escherichia coli K12.. Mol Gen Genet. 1977 Sep 9;154(3):305-9. doi: 10.1007/BF00571287. PubMed ID: 337110 .
4. Kehoe MA, Foster TJ. Genetic analysis of mutations in the transfer genes of pDU202 tra::Tn10 plasmids, caused by the excision of Tn10.. Mol Gen Genet. 1977 Nov 29;157(1):109-18. doi: 10.1007/BF00268693. PubMed ID: 340912 .
5. Chalmers R, Sewitz S, Lipkow K, Crellin P. Complete nucleotide sequence of Tn10.. J Bacteriol. 2000 May;182(10):2970-2. doi: 10.1128/JB.182.10.2970-2972.2000. PubMed ID: 10781570 .
2. Kleckner N, Chan RK, Tye BK, Botstein D. Mutagenesis by insertion of a drug-resistance element carrying an inverted repetition.. J Mol Biol. 1975 Oct 5;97(4):561-75. doi: 10.1016/s0022-2836(75)80059-3. PubMed ID: 1102715 .
3. Foster TJ. Insertion of the tetracycline resistance translocation unit Tn10 in the lac operon of Escherichia coli K12.. Mol Gen Genet. 1977 Sep 9;154(3):305-9. doi: 10.1007/BF00571287. PubMed ID: 337110 .
4. Kehoe MA, Foster TJ. Genetic analysis of mutations in the transfer genes of pDU202 tra::Tn10 plasmids, caused by the excision of Tn10.. Mol Gen Genet. 1977 Nov 29;157(1):109-18. doi: 10.1007/BF00268693. PubMed ID: 340912 .
5. Chalmers R, Sewitz S, Lipkow K, Crellin P. Complete nucleotide sequence of Tn10.. J Bacteriol. 2000 May;182(10):2970-2. doi: 10.1128/JB.182.10.2970-2972.2000. PubMed ID: 10781570 .