Mobile element type:
Integron
Name:
InS21
Synonyms:
Accession:
InS21-AJ311891
Family:
Tn402
Group:
Class 1
First isolate:
Yes
Partial:
Yes
Evidence of transposition:
ND
Host Organism:
Salmonellaenterica subsp. enterica serovar Infantis
Date of Isolation:
2001
Country:
Argentina
Molecular Source:
plasmid pS21
| Name | Coordinates | Direction | Length | Sequence |
|---|---|---|---|---|
| repeat i4 | 10661-10679 | - | 19 | TCAGCGGACG CAGGGAGGA |
| repeat i3 | 10689-10707 | - | 19 | TCAGGCAACG ACGGGCTGC |
| repeat i2 | 10731-10749 | - | 19 | TCAGAAGCCG ACTGCACTA |
| IRi | 10748-10780 | - | 33 | TGTCGTTTTC AGAAGACGGC TGCACTGAAC GTC |
| repeat i1 | 10754-10772 | - | 19 | TCAGAAGACG GCTGCACTG |
| Name | Associated TE | Coordinates | Orientation | Length | Sequence |
|---|---|---|---|---|---|
| attC qacH_sul1 core | InS21 | 704-737 | + | 34 | CCGCTAGCGG GCGGCCGGAA GGTGAATGCT AGGC |
| attC orfD | InS21 | 7610-7657 , 7919-7925 | + | 55 | GAGGTAAGCC GACCGCAGAA TGCGGGTCGG CTTGACCGAA ATGTTAGATA CCAAC |
| attC orfD core | InS21 | 7610-7657 | + | 48 | GAGGTAAGCC GACCGCAGAA TGCGGGTCGG CTTGACCGAA ATGTTAGA |
| attC bla OXA-2 | InS21 | 7926-7988 , 8795-8801 | + | 70 | GTTGAAGTAA CCGGCGCTGC GCGGTTTTAT CGCGCAGCGT CCGAGTTGAC TGCCGGGTTG GGCGCCCAAC |
| attC bla OXA-2 core | InS21 | 7926-7988 | + | 63 | GTTGAAGTAA CCGGCGCTGC GCGGTTTTAT CGCGCAGCGT CCGAGTTGAC TGCCGGGTTG GGC |
| attC AAC(6')-Ib10 | InS21 | 8801-8866 , 9434-9440 | + | 73 | CGTTTGACAT GAGGGGCGGC CAAGGGCGCC AGCCCTTGGA CGTCCCCCTC GATGGAAGGG TTAGGCGCCT AAC |
| attC AAC(6')-Ib core | InS21 | 8801-8866 | + | 66 | CGTTTGACAT GAGGGGCGGC CAAGGGCGCC AGCCCTTGGA CGTCCCCCTC GATGGAAGGG TTAGGC |
ORF Summary
| Name | Associated TE | Coordinates | Orientation | Class | Subclass |
|---|---|---|---|---|---|
| GNAT_fam | InS21 | 104-604 | - | Passenger Gene | Antibiotic Resistance |
| sul1 (ARO:3000410) | InS21 | 732-1571 | - | Passenger Gene | Antibiotic Resistance |
| ORF3-qacEdelta1 | InS21 | 1565-2554 | - | Passenger Gene | Antibiotic Resistance |
| bla CTX-M-2 (ARO:3001865) | InS21 | 2996-3871 | - | Passenger Gene | Antibiotic Resistance |
| tnp | ISCR1 | 4370-5911 | - | Transposase | |
| sul1 (ARO:3000410) | InS21 | 6316-7155 | - | Passenger Gene | Antibiotic Resistance |
| qacEdelta1 (ARO:3005010) | InS21 | 7149-7496 | - | Passenger Gene | Antibiotic Resistance |
| orfD-AXL94324.1 | InS21 | 7601-7891 | - | Passenger Gene | Hypothetical |
| bla OXA-2 (ARO:3001397) | InS21 | 7952-8779 | - | Passenger Gene | Antibiotic Resistance |
| AAC(6')-Ib10 (ARO:3002581) | InS21 | 8861-9415 | - | Passenger Gene | Antibiotic Resistance |
| intI1 | InS21 | 9565-10578 | + | Integron Integrase | Class 1 |
ORF Details
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | GNAT_fam | GNAT_fam | InS21 | 166 | 104-604 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antibiotic Resistance | ||||||||||
| Sequence Family: | Acetyltransf_1 (Pfam:PF00583) | ||||||||||
| Target: | aminoglycoside antibiotic (ARO:0000016) | ||||||||||
| Comment: | putative acetyltransferase ADU64769.1 | ||||||||||
| Protein Sequence: | MDSEEPPNVR VACSGDIDEV VRLMHDAAAW MSAKGTPAWD VARIDRTFAE TFVLRSELLV ASCSDGIVGC CTLSAEDPEF WPDALKGEAA YLHKLAVRRT HAGRGVSSAL IEACRHAART QGCAKLRLDC HPNLRGLYER LGFTHVDTFN PGWDPTFIAE RLELEI |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | sul1 (ARO:3000410) | Sul1 | InS21 | 279 | 732-1571 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antibiotic Resistance | ||||||||||
| Function: | antibiotic target replacement (ARO:0001002) | ||||||||||
| Sequence Family: | sulfonamide resistant sul (ARO:3004238) | ||||||||||
| Transposase Chemistry: | dihydropteroate synthase | ||||||||||
| Target: | sulfonamide antibiotic (ARO:3000282)||sulfone antibiotic (ARO:3003401) | ||||||||||
| Comment: | perfect match to reference sequence for ARO:3000410 | ||||||||||
| Protein Sequence: | MVTVFGILNL TEDSFFDESR RLDPAGAVTA AIEMLRVGSD VVDVGPAASH PDARPVSPAD EIRRIAPLLD ALSDQMHRVS IDSFQPETQR YALKRGVGYL NDIQGFPDPA LYPDIAEADC RLVVMHSAQR DGIATRTGHL RPEDALDEIV RFFEARVSAL RRSGVAADRL ILDPGMGFFL SPAPETSLHV LSNLQKLKSA LGLPLLVSVS RKSFLGATVG LPVKDLGPAS LAAELHAIGN GADYVRTHAP GDLRSAITFS ETLAKFRSRD ARDRGLDHA |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | ORF3-qacEdelta1 | ORF3-qacEdelta1 | InS21 | 329 | 1565-2554 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antibiotic Resistance | ||||||||||
| Protein Sequence: | MKSLNNNFAR VTCMSCPSHC EVKPNASLRV SFCQEYCFCT WPEASRYFSL GIMEGLLKQQ YHYLYLGCVF VDFSISYLRF FTDKKWLDYL YETKLKIVLV CDRHLRPLAN YWYKHHKDIF LIIYQQDNLK SAGDKLKKRF IYQRDAFFHG LSLSTLEFDV LGALIAGEDC NHLARGRHLD IRAVYAAKRR AEKKMGADIN TPCIISPTQI GRYIMKGWLF LVIAIVGEVI ATSALKSSEG FTKLAPSAVV IIGYGIAFYF LSLVLKSIPV GVAYAVWSGL GVVIITAIAW LLHGQKLDAW GFVGMGLIIA AFLLARSPSW KSLRRPTPW |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | bla CTX-M-2 (ARO:3001865) | Bla CTX-M-2 | InS21 | 291 | 2996-3871 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antibiotic Resistance | ||||||||||
| Function: | antibiotic inactivation (ARO:0001004) | ||||||||||
| Sequence Family: | CTX-M beta-lactamase (ARO:3000016) | ||||||||||
| Transposase Chemistry: | antibiotic inactivation (ARO:0001004) | ||||||||||
| Target: | cephalosporin (ARO:0000032) | ||||||||||
| Comment: | strict match to reference sequence for ARO:3001865 (bitscore: 599) | ||||||||||
| Protein Sequence: | MMTQSIRRSM LTVMATLPLL FSSATLHAQA NSVQQQLEAL EKSSGGRLGV ALINTADNSQ ILYRADERFA MCSTSKVMAA AAVLKQSESD KHLLNQRVEI KKSDLVNYNP IAEKHVNGTM TLAELGAAAL QYSDNTAMNK LIAHLGGPDK VTAFARSLGD ETFRLDRTEP TLNTAIPGDP RDTTTPLAMA QTLKNLTLGK ALAETQRAQL VTWLKGNTTG SASIRAGLPK SWVVGDKTGS GDYGTTNDIA VIWPENHAPL VLVTYFTQPE QKAESRRDIL AAAAKIVTHG F |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnp | Tnp | ISCR1 | 513 | 4370-5911 | - |
|---|
| Class: | Transposase | ||||||||||
| Sequence Family: | IS91 tnp | ||||||||||
| Transposase Chemistry: | HUH | ||||||||||
| Comment: | CRof ISCR1 | ||||||||||
| Protein Sequence: | MSLARNATAS QSPTQTNGYE RHQPDQTLLY QLVEQHYPAF KASLEAQGQH LPRYIQQEFN DLLQCGRLEY GFMRVRCEDC HHERLVAFSC KRRGFCPSCG ARRMAESAAL LIDEVFPKEP IRQWVLSFPF QLRFLLARHP QLMGQVLSIV YRTLSTHLIK KAGYTKASAQ TGSVTLIQRF GSALNLNVHY HMLFLDGVYA EDDYGKQRFH RVKAPTYDEL NTLAHTLSHR IARCMEKRGI LERDAENTWL TLEEGEDDTL TQLHGASVTY RIAVGPQQGR KVFTLQTLPG REDKADSSSR VANHAGFSLH AGVMAEAHQR DKLERLCRYI SRPAVSEKRL ALTANGQVRY ELKTPYRNGT THVIFEPLDF IAKLAALVPK PRVNLTRFHG VFVPNSKHRV QVTPAKRGKK PDKSEGLDTN WRDKSPAERH RAMTWMQRLK RVFNIDIEVC EHCGGHVKVI ASIEDPKVIE QILKHLKQKT AKANAAKQRE LPPERAPPLT PSLFDPSQSR LFD |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | sul1 (ARO:3000410) | Sul1 | InS21 | 279 | 6316-7155 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antibiotic Resistance | ||||||||||
| Function: | antibiotic target replacement (ARO:0001002) | ||||||||||
| Sequence Family: | sulfonamide resistant sul (ARO:3004238) | ||||||||||
| Transposase Chemistry: | dihydropteroate synthase | ||||||||||
| Target: | sulfonamide antibiotic (ARO:3000282)||sulfone antibiotic (ARO:3003401) | ||||||||||
| Comment: | perfect match to reference sequence for ARO:3000410 | ||||||||||
| Protein Sequence: | MVTVFGILNL TEDSFFDESR RLDPAGAVTA AIEMLRVGSD VVDVGPAASH PDARPVSPAD EIRRIAPLLD ALSDQMHRVS IDSFQPETQR YALKRGVGYL NDIQGFPDPA LYPDIAEADC RLVVMHSAQR DGIATRTGHL RPEDALDEIV RFFEARVSAL RRSGVAADRL ILDPGMGFFL SPAPETSLHV LSNLQKLKSA LGLPLLVSVS RKSFLGATVG LPVKDLGPAS LAAELHAIGN GADYVRTHAP GDLRSAITFS ETLAKFRSRD ARDRGLDHA |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | qacEdelta1 (ARO:3005010) | QacEdelta1 | InS21 | 115 | 7149-7496 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antibiotic Resistance | ||||||||||
| Function: | antibiotic efflux (ARO:0010000) | ||||||||||
| Sequence Family: | major facilitator superfamily (MFS) antibiotic efflux pump (ARO:0010002) | ||||||||||
| Target: | disinfecting agents and antiseptics(ARO:3005386) | ||||||||||
| Comment: | subunit of the qac multidrug efflux pump||perfect match to reference sequence for ARO:3005010 (bitscore:219) | ||||||||||
| Protein Sequence: | MKGWLFLVIA IVGEVIATSA LKSSEGFTKL APSAVVIIGY GIAFYFLSLV LKSIPVGVAY AVWSGLGVVI ITAIAWLLHG QKLDAWGFVG MGLIIAAFLL ARSPSWKSLR RPTPW |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | orfD-AXL94324.1 | OrfD-AXL94324.1 | InS21 | 96 | 7601-7891 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Hypothetical | ||||||||||
| Function: | unknown | ||||||||||
| Protein Sequence: | MFIQTAFSFS NAVQRWVCRF SGLRLVALRW FVVFLASGPC VASASSYFFR SAVPPRWHRA LSQLAPVAKL RLSVLASGSN ISVKPTRILR SAYLAR |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | bla OXA-2 (ARO:3001397) | Bla OXA-2 | InS21 | 275 | 7952-8779 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antibiotic Resistance | ||||||||||
| Function: | antibiotic inactivation (ARO:0001004) | ||||||||||
| Sequence Family: | OXA beta-lactamase (ARO:3000017) | ||||||||||
| Transposase Chemistry: | antibiotic inactivation (ARO:0001004) | ||||||||||
| Target: | cephalosporin (ARO:0000032)||penam (ARO:3000008)||carbapenem (ARO:0000020) | ||||||||||
| Comment: | strict match to reference sequence for ARO:3001397 (bitscore: 561) | ||||||||||
| Protein Sequence: | MAIRIFAILS SIFSLATFAH AQEGTLERSD WRKFFSEFQA KGTIVVADER QADRAMLVFD PVRSKKRYSP ASTFKIPHTL FALDAGAVRD EFQIFRWDGV NRGFAGHNQD QDLRSAMRNS TVWVYELFAK EIGDDKARRY LKKIDYGNAD PSTSNGDYWI EGSLAISAQE QIAFLRKLYR NELPFRVEHQ RLVKDLMIVE AGRNWILRAK TG*EGRMGWW VGWVEWPTGS VFFALNIDTP NRMDDLFKRE AIVRAILRSI EALPPNPAVN SDAAR |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | AAC(6')-Ib10 (ARO:3002581) | AAC(6')-Ib10 | InS21 | 184 | 8861-9415 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antibiotic Resistance | ||||||||||
| Function: | antibiotic inactivation (ARO:0001004) | ||||||||||
| Sequence Family: | AAC(6') (ARO:3000345) | ||||||||||
| Transposase Chemistry: | aminoglycoside acetyltransferase | ||||||||||
| Target: | aminoglycoside antibiotic (ARO:0000016) | ||||||||||
| Comment: | strict match to reference sequence for ARO:3002581 (bitscore: 377) | ||||||||||
| Protein Sequence: | MTNSNDSVTL RLMTEHDLAM LYEWLNRSHI VEWWGGEEAR PTLADVQEQY LPSVLAQESV TPYIAMLNGE PIGYAQSYVA LGSGDGWWEE ETDPGVRGID QLLANASQLG KGLGTKLVRA LVELLFNDPE VTKIQTDPSP SNLRAIRCYE KAGFERQGTV TTPDGPAVYM VQTRQAFERT RSDA |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | intI1 | IntI1 | InS21 | 337 | 9565-10578 | + |
|---|
| Class: | Integron Integrase | ||||||||||
| Subclass: | Class 1 | ||||||||||
| Sequence Family: | Class 1 Integron Tyrosine Integrase | ||||||||||
| Transposase Chemistry: | Tyrosine | ||||||||||
| Protein Sequence: | MKTATAPLPP LRSVKVLDQL RERIRYLHYS LRTEQAYVNW VRAFIRFHGV RHPATLGSSE VEAFLSWLAN ERKVSVSTHR QALAALLFFY GKVLCTDLPW LQEIGRPRPS RRLPVVLTPD EVVRILGFLE GEHRLFAQLL YGTGMRISEG LQLRVKDLDF DHGTIIVREG KGSKDRALML PESLAPSLRE QLSRARAWWL KDQAEGRSGV ALPDALERKY PRAGHSWPWF WVFAQHTHST DPRSGVVRRH HMYDQTFQRA FKRAVEQAGI TKPATPHTLR HSFATALLRS GYDIRTVQDL LGHSDVSTTM IYTHVLKVGG AGVSSPLDAL PPLTSER |
||||||||||
| TnCentral Accession | TE Name | Type | Coordinates | Strand | Length |
|---|---|---|---|---|---|
| ISCR1-L06822 | ISCR1 | transposon | 4138-6291 | + | 2154 |