Mobile element type:
Integron
Name:
In7
Synonyms:
Accession:
In7-L06418
Family:
Tn402
Group:
Class1
First isolate:
ND
Partial:
Yes
Evidence of transposition:
ND
Host Organism:
Escherichia coli
Date of Isolation:
1986
Country:
Molecular Source:
plasmid pDGO100
ORF Summary
| Name | Associated TE | Coordinates | Orientation | Class | Subclass |
|---|---|---|---|---|---|
| qacEdelta1 (ARO:3005010) | In7 | 1194-1541 | - | Passenger Gene | Antibiotic Resistance |
| dfrA10 (ARO:3003011) | In7 | 1578-2141 | - | Passenger Gene | Antibiotic Resistance |
| tnp | ISCR1 | 2523-4064 | - | Transposase | |
| qacEdelta1 (ARO:3005010) | In7 | 5302-5649 | - | Passenger Gene | Antibiotic Resistance |
ORF Details
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | qacEdelta1 (ARO:3005010) | QacEdelta1 | In7 | 115 | 1194-1541 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antibiotic Resistance | ||||||||||
| Function: | antibiotic efflux (ARO:0010000) | ||||||||||
| Sequence Family: | major facilitator superfamily (MFS) antibiotic efflux pump (ARO:0010002) | ||||||||||
| Target: | disinfecting agents and antiseptics(ARO:3005386) | ||||||||||
| Comment: | subunit of the qac multidrug efflux pump||perfect match to reference sequence for ARO:3005010 (bitscore:219) | ||||||||||
| Protein Sequence: | MKGWLFLVIA IVGEVIATSA LKSSEGFTKL APSAVVIIGY GIAFYFLSLV LKSIPVGVAY AVWSGLGVVI ITAIAWLLHG QKLDAWGFVG MGLIIAAFLL ARSPSWKSLR RPTPW |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | dfrA10 (ARO:3003011) | DfrA10 | In7 | 187 | 1578-2141 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antibiotic Resistance | ||||||||||
| Function: | antibiotic target replacement (ARO:0001002) | ||||||||||
| Sequence Family: | trimethoprim resistant dihydrofolate reductase dfr (ARO:3001218) | ||||||||||
| Target: | diaminopyrimidine antibiotic (ARO:3000171) | ||||||||||
| Protein Sequence: | MNISLIFANE LITRAFGNQG KLPWQFIKED MQFFQKTTEN SVVVMGLNTW RSLPKMKKLG RDFIVISSTI TEHEVLNNNI QIFKSFESFL EAFRDTTKPI NVIGGVGLLS EAIEHASTVY MSSIHMVKPV HADVYVPVEL MNKLYSDFKY PENILWVGDP IDSVYSLSID KFVRPASLVG VPNDINT |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnp | Tnp | ISCR1 | 513 | 2523-4064 | - |
|---|
| Class: | Transposase | ||||||||||
| Sequence Family: | IS91 tnp | ||||||||||
| Transposase Chemistry: | HUH | ||||||||||
| Comment: | CRof ISCR1 | ||||||||||
| Protein Sequence: | MSLARNATAS QSPTQTNGYE RHQPDQTLLY QLVEQHYPAF KASLEAQGQH LPRYIQQEFN DLLQCGRLEY GFMRVRCEDC HHERLVAFSC KRRGFCPSCG ARRMAESAAL LIDEVFPKEP IRQWVLSFPF QLRFLLARHP QLMGQVLSIV YRTLSTHLIK KAGYTKASAQ TGSVTLIQRF GSALNLNVHY HMLFLDGVYA EDDYGKQRFH RVKAPTYDEL NTLAHTLSHR IARCMEKRGI LERDAENTWL TLEEGEDDTL TQLHGASVTY RIAVGPQQGR KVFTLQTLPG REDKADSSSR VANHAGFSLH AGVMAEAHQR DKLERLCRYI SRPAVSEKRL ALTANGQVRY ELKTPYRNGT THVIFEPLDF IAKLAALVPK PRVNLTRFHG VFAPNSKHRV QVTPAKRGKK PDKSEGLDTN WRDKSPAERH RAMTWMQRLK RVFNIDIEVC EHCGGHVKVI ASIEDPKVIE QILKHLKQKT AKANAAKQRE LPPERAPPLT PSLFDPSQSR LFD |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | qacEdelta1 (ARO:3005010) | QacEdelta1 | In7 | 115 | 5302-5649 | - |
|---|
| Class: | Passenger Gene | ||||||||||
| Subclass: | Antibiotic Resistance | ||||||||||
| Function: | antibiotic efflux (ARO:0010000) | ||||||||||
| Sequence Family: | major facilitator superfamily (MFS) antibiotic efflux pump (ARO:0010002) | ||||||||||
| Target: | disinfecting agents and antiseptics(ARO:3005386) | ||||||||||
| Comment: | subunit of the qac multidrug efflux pump||perfect match to reference sequence for ARO:3005010 (bitscore:219) | ||||||||||
| Protein Sequence: | MKGWLFLVIA IVGEVIATSA LKSSEGFTKL APSAVVIIGY GIAFYFLSLV LKSIPVGVAY AVWSGLGVVI ITAIAWLLHG QKLDAWGFVG MGLIIAAFLL ARSPSWKSLR RPTPW |
||||||||||
| TnCentral Accession | TE Name | Type | Coordinates | Strand | Length |
|---|---|---|---|---|---|
| ISCR1-L06822 | ISCR1 | transposon | 2291-4444 | + | 2154 |
1. Cameron FH, Groot Obbink DJ, Ackerman VP, Hall RM.
Nucleotide sequence of the AAD(2'') aminoglycoside adenylyltransferase determinant aadB. Evolutionary relationship of this region with those surrounding aadA in R538-1 and dhfrII in R388.. Nucleic Acids Res. 1986 Nov 11;14(21):8625-35. doi: 10.1093/nar/14.21.8625. PubMed ID:
3024112
.
2. Hall RM, Vockler C. The region of the IncN plasmid R46 coding for resistance to beta-lactam antibiotics, streptomycin/spectinomycin and sulphonamides is closely related to antibiotic resistance segments found in IncW plasmids and in Tn21-like transposons.. Nucleic Acids Res. 1987 Sep 25;15(18):7491-501. doi: 10.1093/nar/15.18.7491. PubMed ID: 2821509 .
3. Hall RM, Stokes HW. The structure of a partial duplication in the integron of plasmid pDGO100.. Plasmid. 1990 Jan;23(1):76-9. doi: 10.1016/0147-619x(90)90047-g. PubMed ID: 2349283 .
4. Parsons Y, Hall RM, Stokes HW. A new trimethoprim resistance gene, dhfrX, in the In7 integron of plasmid pDGO100.. Antimicrob Agents Chemother. 1991 Nov;35(11):2436-9. doi: 10.1128/AAC.35.11.2436. PubMed ID: 1804022 .
5. Stokes HW, Tomaras C, Parsons Y, Hall RM. The partial 3'-conserved segment duplications in the integrons In6 from pSa and In7 from pDGO100 have a common origin.. Plasmid. 1993 Jul;30(1):39-50. doi: 10.1006/plas.1993.1032. PubMed ID: 8378445 .
6. Partridge SR, Hall RM. In34, a complex In5 family class 1 integron containing orf513 and dfrA10.. Antimicrob Agents Chemother. 2003 Jan;47(1):342-9. doi: 10.1128/AAC.47.1.342-349.2003. PubMed ID: 12499211 .
2. Hall RM, Vockler C. The region of the IncN plasmid R46 coding for resistance to beta-lactam antibiotics, streptomycin/spectinomycin and sulphonamides is closely related to antibiotic resistance segments found in IncW plasmids and in Tn21-like transposons.. Nucleic Acids Res. 1987 Sep 25;15(18):7491-501. doi: 10.1093/nar/15.18.7491. PubMed ID: 2821509 .
3. Hall RM, Stokes HW. The structure of a partial duplication in the integron of plasmid pDGO100.. Plasmid. 1990 Jan;23(1):76-9. doi: 10.1016/0147-619x(90)90047-g. PubMed ID: 2349283 .
4. Parsons Y, Hall RM, Stokes HW. A new trimethoprim resistance gene, dhfrX, in the In7 integron of plasmid pDGO100.. Antimicrob Agents Chemother. 1991 Nov;35(11):2436-9. doi: 10.1128/AAC.35.11.2436. PubMed ID: 1804022 .
5. Stokes HW, Tomaras C, Parsons Y, Hall RM. The partial 3'-conserved segment duplications in the integrons In6 from pSa and In7 from pDGO100 have a common origin.. Plasmid. 1993 Jul;30(1):39-50. doi: 10.1006/plas.1993.1032. PubMed ID: 8378445 .
6. Partridge SR, Hall RM. In34, a complex In5 family class 1 integron containing orf513 and dfrA10.. Antimicrob Agents Chemother. 2003 Jan;47(1):342-9. doi: 10.1128/AAC.47.1.342-349.2003. PubMed ID: 12499211 .