Mobile element type:
Insertion sequence
Name:
IS1326::IS1353
Synonyms:
Accession:
IS1326_IS1353-AF071413
Family:
IS21
Group:
First isolate:
ND
Partial:
ND
Evidence of transposition:
ND
Host Organism:
Shigella flexneri
Date of Isolation:
1950s
Country:
Japan
Molecular Source:
plasmid NR1 (R100)
| Name | Coordinates | Direction | Length | Sequence |
|---|---|---|---|---|
| IRL | 1-26 | + | 26 | TGTTGAGTTG CATCTAAAAT TGACCC |
| IRR | 4061-4086 | - | 26 | TGTTGATTTG CACCCAAATT TGACCC |
ORF Summary
| Name | Associated TE | Coordinates | Orientation | Class | Subclass |
|---|---|---|---|---|---|
| tnp | IS1353 | 57-1601 | - | Transposase | |
| istA | IS1326::IS1353 | 1724-3247 | + | Transposase | None |
| istB | IS1326::IS1353 | 3234-4019 | + | Accessory Gene | ATPase Transposition Helper |
ORF Details
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | tnp | Tnp | IS1353 | 514 | 57-1601 | - |
|---|
| Class: | Transposase | ||||||||||
| Function: | transposase | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Protein Sequence: | MYSYEDRLRA VRLYLKLGRR MSATLRQLGY PTKNSLKAWL AEFERNQDLR RGYQRIKRQY TDEQKQRAVD HYIEQGYCLS HTIRSLGYPS REALRAWIRD LRPEFARTVV GSSAPTVARS RLEKQQAVIA LNLRVGSAKD VADTVGVSRP TLYNWQHRLL GKVPLKPMTK KKGDTSLEQR HEALLRELAE LESQNQRLRM ENAILEKASE LIKKDMGINP LELTSREKTK VVDALRVTFP LANLLCGLKL ARSTYFYQRL RQTRPDKYTQ VREVIRTIFE DNYRCYGYRR IDSALRLGGM RVSEKVVRRL MAQERLVVRT PRRRRFSAYA GDPTPAVPNL LNRDFHASAP NTKWLTDLTE IHIPAGKVYV SPIVDCFDGL VVAWNIGTSP DANLVNTMLD HAVRTLRPGE HPVIHSDRGS HYRWPAWIRR TENAQLTRSM SKKGCSPDNA ACEGFFGRLK TELIYPRNWQ HVTLKDLMTR IDAYIHWYNE RRIKVSLGGR SPIEYRHAVG LMSV |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | istA | IstA | IS1326::IS1353 | 507 | 1724-3247 | + |
|---|
| Class: | Transposase | ||||||||||
| Transposase Chemistry: | DDE | ||||||||||
| Protein Sequence: | MINVAILSAI RRWHFRDGAS IREIARRSGL SRNTVRKYLQ SKVVEPQYPA RDSVGKLSPF EPKLRQWLST EHKKTKKLRR NLRSMYRDLV ALGFTGSYDR VCAFARQWKD SEQFKAQTSG KGCFIPLRFA CGEAFQFDWS EDFARIAGKQ VKLQIAQFKL AHSRAFVLRA YYQQKHEMLF DAHWHAFQIF GGIPKRGIYD NMKTAVDSVG RGKERRVNQR FTAMVSHYLF DAQFCNPASG WEKGQIEKNV QDSRQRLWQG APDFQSLADL NVWLEHRCKA LWSELRHPEL DQTVQEAFAD EQGELMALPN AFDAFVEQTK RVTSTCLVHH EGNRYSVPAS YANRAISLRI YADKLVMAAE GQHIAEHPRL FGSGHARRGH TQYDWHHYLS VLQKKPGALR NGAPFAELPP AFKKLQSILL QRPGGDRDMV EILALVLHHD EGAVLSAVEL ALECGKPSKE HVLNLLGRLT EEPPPKPIPI PKGLRLTLEP QANVNRYDSL RRAHDAA |
||||||||||
| Gene Name | Protein Name | Associated TE | Gene Length | Coordinates | Strand | istB | IstB | IS1326::IS1353 | 261 | 3234-4019 | + |
|---|
| Class: | Accessory Gene | ||||||||||
| Subclass: | ATPase Transposition Helper | ||||||||||
| Function: | stimulates transposition | ||||||||||
| Protein Sequence: | MMQHEGHVRI LKSLKLFGMA HAIEELGNQN SPAFNQALPM LDSLIKAEVA EREVRSVNYQ LRVAKFPVYR DLVGFDFSQS LVNEATVKQL HRCDFMEQAQ NVVLIGGPGT GKTHLATAIG TQAVMHLNRR VRFFSTVDLV NALEQEKSSG RQGQIANRLL YADLVILDEL GYLPFSQTGG ALLFHLLSKL YEKTSVILTT NLSFSEWSRV FGDEKMTTAL LDRLTHHCHI LETGNESYRF KHSSTQNKQE EKQTRKLKIE T |
||||||||||
| TnCentral Accession | TE Name | Type | Coordinates | Strand | Length |
|---|---|---|---|---|---|
| IS1353-AF071413 | IS1353 | insertion sequence | 29-1642 | + | 1614 |
| Name | Associated TE | Coordinates | Direction | Length | Sequence |
|---|---|---|---|---|---|
| IRR | IS1353 | 29-41 | + | 13 | TGGGGGTGCG GAC |
| IRL | IS1353 | 1631-1642 | - | 12 | TGGGGTGCGG AC |