ISfinder Mirror Site

ISNisp5

Family IS1
Group ISMhu11

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NZ_AAMY01000028	ND	Nitrobacter sp.	Nitrobacter sp. Nb-311A

DNA section

IS Length : 3580 bp

Ends

IR Length : 18

IRL : GGTAGTGGGTCAGTTTGATTGTGGCAGCCATAGTGCCTAGGAATCGGAGT
IRR : GGTAGTGGGTCAGTTTGAAAAATGACCCCGAAAGAAACGTCTTGGTTAAT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
ATGACCTACTATTG	CGGCCTTG	ACTCCAAGAAC	8

DNA sequence

GGTAGTGGGTCAGTTTGATTGTGGCAGCCATAGTGCCTAGGAATCGGAGTGATTCAGGGCCATATTCGGGGCATGTCGTCATCTCATCCAAAGCGCCCCC
GCGATCCAAATCAGCTGGCGAAAGCTATCGTGGATATCGCCACAGGCAGCCTGCCACCTGAGGTTGACTCGGAACCTTCGCCAGCCTCACAGTTGGGCAG
AATTAGGGGGCTCAAAGGCGGCCGCGCCCGCGCTGACAAACTCAGCGACGAAAGGCGTAGAGAGATCGCCCGTGAAGCCGCCGCTGCGAGGTGGCGTAAA
GATGACGGCGGAAATAGCGATCCTGAATAAGTCGGCTATAGCGTTAGCCGCTGATAGCGCAGTTACAATTGGGACCAGCGACCAAGAAGACAAAACTTAC
GATTCAGCCGACAAGCTTTTCGAATTATGTCGTCATGACCCAATCGGGATCATGGTTTATAATGGTCTCAGTTTTGCTGAGGCCCCGCTGCAGTCGTTGA
TTAAGCGCTTTCGAAATGAATGTGGCCGTTTCGATAGCGTCAAAGAAGCTGCCTTCCAGTTTTTGCGTCACCTCAACGATTTTGGCGTCAGTTCCCCTGA
CAGGGTGAAAGATGATGGGATCAGGCGGTTGGCCATGCCCATCATCGATCAGATTTTCAAACGCTTCCAGGCAGATTTGCAGAAGAGGCTAATGGACCAC
AAGCCTCAGGAAGAAATTGATTGGGCCGCTACTACGAACGACATGTTGGATACGGCAATAGATGTCTGGGATAGAATATATAAGCGAAGAAAAGACGCGG
AGTTCATTGGAGAAGGGCCGGTTGTTATTTCCGAGCACATCGAACAGGAGCTTGTTAGCCTAGTCGCGAATCATTCCCCAGGAAACGAAGCGCAAAAGGC
GCGGATACTTGCAATTCTAAAACTTGCATTGCGGAAAGATTTGCTATCGGGCGGCTACACGGGAATCGTGATAGCGGGATTCGGCAAGAAAGAGCTCTTC
CCAACGCTTCTGTCTTTCGAGATAGATGGAATGGTAGGGGATCGATTGAAGTACGTTGTCCGAAACGAGACGGATATCGATAGAGACGGGCCGCGAGCGG
CGATCATACCCTTCGCTCAGCGGGACATGGTCGATCGCTTCTTGTACGGCTTAGACGACCAGATCGAACGATACCTGACCCAATTCTCACAAAGCACAAT
AACAAAAGTGCGAGAAGCGATCATAGGTCACATTTCTTTCGAAGACGATGATGATAAAAAAGGCTTCGAAGAAGCAGTACAGGATGCTGAGGAGGCTTAC
ATATCCGGACTGAAAACCGGGGCCTTCGAGGAAATCAAAAAGAAATCTCGGTCTGAAATTGAGAGTCTAATTGAGTTCATGCCAAAGCCAGAATTGGCAA
AAATGGCCGAGGCATTAGTAAATTTAACGTCGATCAAAAAACGCGTGTCTCGGGGAATGGATACAGTAGGAGGGCCTATTGATGTGGCGGTGATATCAGA
AGCTGAGGGGTTCGTTTGGGTAAAGCGAAAGCACTATTTCCCGTCAGAATTGAACCCAAGGTATCTGCACCGCGTTGGACAGGCAGTTTGTCAGGAGGAC
AACAATGCACATCATGCAAAATCGAGCGCTGGAAGCTCTCGAAAGGGAAGAAAGACGCCAGCTAGGAAAGGAGAAGGTGCCGGAGGGGCTTCAAGAGGTA
ATTAAACAGACGCGTGAAGCAATCGATAAGGCGCTAGATAGACCGGCTATAAAAAATAAGGCACACTCCAAAATATCCCATAATGCTTGACAGTTAAGCA
TAAGGGTCATTATGATCCGGCCTATGAACAGGCTGGACCGAAAGACCCGCGCCCAAATCCTTCACCTCCTTTGTGAGGGGCAATCTATCCGCGCCGTGAC
GCGGCTGACCGGATGCAGCAAAAACACCGTCGCGAAGTTGCTGGTCGAAGCCGGTCATGCCTGCGCTGCCTACCAGGACAAGGCGCTTCGCAACCTGTCC
TGTAAGCGTGTCCAGATGGATGAAATCTGGTCGTTCGTTTACGCCAAGAACGCCAACGTGAAGGACGCCAAAGCCGCTCCGGCGACCGCAGGCGATGTTT
GGACGTGGACAGCGATTGATGCCGACTCAAAGCTGATCGTTAGCTGGCTGCTCGGTGCTCGCGATATGGACGCTGCTCTGGCCTTCACTGCGGACTTACG
GAGCAGATTAAGCAATCGCGTCCAGCTCACCAGTGATGGCCATAGGCCCTATCTGACCGCCGTTGACGCTGTATTCGGTGACGATGTTGACTATGCGATG
CTGGTGAAGCTGTACGGCGCGGACCCACAGGCTGAGGTCCGATATAGCCCCGCCAAGTGCATCGGTGCGGAAAAGAAGCCGAAGATTGGCAGCCCCGATC
AGAAGCATATCAGCACATCATACGTTGAGCGCAGCAACCTCACGATGCGGATGCATATGCGCCGGTTTACCCGGCTCACCAATGCGTTCTCGAAGAAGGT
GGAGAACCACGCCGCAGCAATTGCGCTCCATACGATGTATTACAACTTCGTCCGCATCCATCAGACGCTCAAGGTCACGCCCGCAATGGCCGCTGGCGTA
ACTGACAAGCTTTGGGAGATGGAAGATTTGGTCGCCGTGCTAGAGCAATGGGAGCTGGCGAACTTCAAGCCAGAATATCAGTTCGTGGTCCGGCGGTATG
CAATCGGGAAGGGCCATTCCGTCAGCGTGCTTTGGCGCGGCGGGGAAGTCGATACGATTTTCGGATTTGATAAAGAAGCTGACGCGTTAGAGTGGATTAG
GGATAAGTCTAGAGCGTGGCTCTTAGAGAGGCCGGTGCGAGCCTAAGCCCCCTTGTGAAGCCTTGCTTAAAGTGGTTTAACGCCCTCCTGTTGCCCTAAA
GGCACGTCAACTTGCAAAGAATCAGGTAATTTTGAGACGGCCCCTAGACGCGGGGATTACTCTGGAGGGTTTTATGTCCGTCGTGGCTCGTCGGAAAAAA
GTTTCCCGAACGAAAAAGCCCATTCGAAAAGAATATAAAATTGAGTTGGTTGGAAAGCGTAGGTCTGACGGAAGCTTGTTTGTTACAAGCCCAAATCTAG
GACCTTTCAGCGCTGTTCTCCGAACTGGCCGATGGGAAGATGTGATCGGCTTTCTGAAAAAATTTTTAGAAGTAAATATTGGTCGCGTAGTTGAGTTGCG
CTTGATACATGACGCGTCTGAATTGGTGAGTGATTCGGAAGAGCTACCCGAAATACCGCCAGCATATGTTGTAGCGGAGTTAACCCACCAACGTGCTAGT
GCCCGTTCAACAAGCTAGAGCACAACTAAAGGCCGCTGGGTTCAAACGCCGGACGGATGTCCGCGACGAAAAGGGTGAATGGTGGGCGCATCCAAGTGGG
TGGCCCGTTCCTTTCCTGCAGTACATCGGCCTAGATAAAGGCAACTTTGACGCTGACCAACTTGCCTACACGTTGGCAATGAGTGGCCCATTTCGTCCGG
GCAAGACGATCGGCCCCAACGCTTCCTTGAATTAACCAAGACGTTTCTTTCGGGGTCATTTTTCAAACTGACCCACTACC

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
1102 bp	467 aa	302	1403	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MTAEIAILNKSAIALAADSAVTIGTSDQEDKTYDSADKLFELCRHDPIGIMVYNGLSFAEAPLQSLIKRFRNECGRFDSVKEAAFQFLRHLNDFGVSSPD
RVKDDGIRRLAMPIIDQIFKRFQADLQKRLMDHKPQEEIDWAATTNDMLDTAIDVWDRIYKRRKDAEFIGEGPVVISEHIEQELVSLVANHSPGNEAQKA
RILAILKLALRKDLLSGGYTGIVIAGFGKKELFPTLLSFEIDGMVGDRLKYVVRNETDIDRDGPRAAIIPFAQRDMVDRFLYGLDDQIERYLTQFSQSTI
TKVREAIIGHISFEDDDDKKGFEEAVQDAEEAYISGLKTGAFEEIKKKSRSEIESLIEFMPKPELAKMAEALVNLTSIKKRVSRGMDTVGGPIDVAVISE
AEGFVWVKRKHYFPSELNPRYLHRVGQAVCQEDNNAHHAKSSAGSSRKGRKTPARKGEGAGGASRGN

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
1035 bp	344 aa	1510	2544	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MIRPMNRLDRKTRAQILHLLCEGQSIRAVTRLTGCSKNTVAKLLVEAGHACAAYQDKALRNLSCKRVQMDEIWSFVYAKNANVKDAKAAPATAGDVWTWT
AIDADSKLIVSWLLGARDMDAALAFTADLRSRLSNRVQLTSDGHRPYLTAVDAVFGDDVDYAMLVKLYGADPQAEVRYSPAKCIGAEKKPKIGSPDQKHI
STSYVERSNLTMRMHMRRFTRLTNAFSKKVENHAAAIALHTMYYNFVRIHQTLKVTPAMAAGVTDKLWEMEDLVAVLEQWELANFKPEYQFVVRRYAIGK
GHSVSVLWRGGEVDTIFGFDKEADALEWIRDKSRAWLLERPVRA

Blast result :

Comments

The second ORF is the transposase. ISNisp5 is 48% aa similar to ISMhu11.The first ORF is a passenger gene.

References

1] Waterbury,J., Ferriera,S., Johnson,J., Kravitz,S., Halpern,A., Remington,K., Beeson,K., Tran,B., Rogers,Y.-H., Friedman,R. and Venter,J.C. (2006) Direct submission GenBank.