ISfinder Mirror Site

ISBun1

Family IS1595
Group ISNwi1

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NZ_AAYH02000038	ND	Bacteroides uniformis	Bacteroides uniformis ATCC 8492

DNA section

IS Length : 2700 bp

Ends

IR Length : 28/30

IRL : GGTAACTATATACCACTTTGCACTAAATATTAAATTCAATAAAGAATAGA
IRR : GGTAACTATATACCATTTTGCACTAAAAATGCTTGGGCATATAGTTACCT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
AGAGAAAACTCAAT	AAAAAAAG	AAAGGACAAAAAATGA	8

DNA sequence

GGTAACTATATACCACTTTGCACTAAATATTAAATTCAATAAAGAATAGAAAGTTATGGAAATGAACGAGGAAAGTATTAAAAATTTATGGGTAATAGTA
GAAAAGACCCATAAGCAAGTTTTAGCGATGAAGTTTTTAGGCGAATTTAAAGCTTATGTAGTAAGTGGGTTTTCAACGAAGACAAGGGATAACCCTTTTA
ATGAAGCTTACAATGCGATTGATATTACAGATATATCGGTTAACTTGCCTATTTTACCGTCGGAATTAAACCCTCAATCTTTTGAAGAAAAGCTACGAGG
ACGTTCTGTAAAGAATTTCAAATTTGGTGGTGATGATTATTTTTGGTTGATTAAATCTGGTAAAACAGAATATTTATAAGAAAAATATACTGTTTTAGTT
GGATTTATGAAAAAATATTTGTATATTTAGATGTAAAAGGAGGAATATATTATGATAGACGTCAATTCGTTTAATTCATTGTTTGAACTGTTAGAAGCAT
TTCCTACTGAACAGTCTTGTATCGAATATTTAAAGAAAGTACGATGGGGTGAAAAGGTGAAATCTCCTTTTAGCCCTGGCTCTAAAGTGTATGTATGTGG
AAATAATACATATTTATGTAAGGACACCGGGAGAAGGTTTAATGTAAAAACGGGAACCATTTTTGAGAATACTAAAATGTCGTTGCGAAAGTGGTTTATG
GCCGTATGGATGGTTCTTTCAAACAAGAAAGGAATATCATCTTTACAATTAGCAAGGGATATAAAAGTGACCCAGAAAACAGCCTGGTATGTGTTACAAC
GGATAAGAATATGTTTGGTATGTGAAAATCAAGGAAAATTGCATAATGAAGTAGAAGCCGACGAAACCTTTGTCGGAGGCAAAAATAAGAATCGGCATAA
TGACAAGAAAGTTAAGGCTTGCCAGGGACGTTCATTCAAGGATAAGACCCCGGTTCTTGGAATGGTAGAAAGAAAAGGAAAGGTTATCGGGATAGTTATC
AAGAATACGTCCAAAGAGGAACTCACTCCTAAAATATTGGAGTTTGTTGATAGAAACGCTATATTGTACACTGACGAATGGTACGGATATAATGAGATAG
ACAAAATGTATTATCACTATTCCATAGACCACAGCAAGAAACAATATGGGTACGGAAGAATCAACACTAATACCATTGAGGGTTTTTGGACTTTATTAAA
AAGAGGATATGTAGGAATATACCACTATATGAGTTCTAAGCATCTTCAAAGATATGTTAATGAATTTGTGTTCCGCTATAATACACGAAAAATCAGCGAG
AATTCAAGATTTAATTTGTTACTTTGCAATATGAATTATCATATTACATATAAGAAATTGACAGCATGAGTAAAAAGGAAATGACAATAGGAGAAGAATT
TGAAAACTTTATGAGTTTTGCAATGCGATATAATTTTAAGAAAAAAAAGAAAGTAAAATTATATAAACCAACTGAAATTGCAAAAATATATCGTGAAAAT
GGAATGACGGAAGAAATGCTGTATAAGCGTTGTATAGGATTGGGATGTACAGAAGAGGAAGCAAAGATGTTGGTACAAAAATGCTTCCACCCTACAGAAA
AAGATTTAGAACTTGAAAGATTATAATAAAAGAATACCTTTATTTAGATTCTTTTTCTTTATTCTCGTTTTTCAAATCTTCCTCATAATCTTTTAAATAA
GAATCTTCATACATAATTCCGGGAGTGCCAATAATCTCTTCTTTGATTTTTAATTCACCACAGTGAATACATTTAAAGTAAGCATTATGCTCTTCCCAAA
CATGGTTGCATTTTTTGTTCTCCATAATATTTTCTTATTTGTTTGACATTGCAAATGTAGCAAATATCACTATATTTGCAAATGTTCTATAGAACAAACG
TTTTAGAGAAAATATTCGGGAGTGCCTTACTTCGTTAAAATCGCCAGTTTTAACACGCACACAAGGCACAAGCCCTCACTGTACCAAGTGGGTTGCTTGT
CTGCGTGTGCATAGGTCTCTGGCGATACCTTACGAAGTTGTGGACTGTGACCCACTTCCTTTTAGGTACAGCCTTTATAGGTCACGTGAAGGCAGAAATA
CGAATAGTTATGAAAAAGTTTGTATTATTGATTTTGTTTGTTTTTTCTTGTACGTTATCATCTTATGCACAATTCGATAACGGATTACAATTTAGGGATT
CTCTTGCAGGATGGACTGCAAGACATGCAAAAATTAGTGAGATAGAACCAATTCTAAAAAAATACAATATTGATTCTTCTGGAAAGCGAATGATTATAAA
AATAGATGAGAATAATATTTTTAGAATTAGATGTAACGTTGATTCACAAGATGAAATAGAGTGGATAAGTATGACAACTTTATATGATTCAACGGATTCC
GGAATGAAAGCTGATATTATGGTAATGACTACACTTTTAGAAAATTCTAAATATTTAGGATTGAAATTTAAAGAAGAAGCTTATTTTCAAATGAGTAGTG
AAAACAACATGTTTTTTGTTTCAGAAAAATTGGGGATTACTGTTGTTCTTACTACATGCTCAATGTCTCGAAGATATGGATGTCCTACTTTGGAAATATC
ATTTATAAAAGGAACTGATTATAAAAAATGGATTAAACAATAAATAATAAAGGTAACTATATGCCCAAGCATTTTTAGTGCAAAATGGTATATAGTTACC

Protein section

ORF number : 5

ORF 1

Length		Begin	End	Strand	Fusion ORF
324 bp	107 aa	56	379	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MEMNEESIKNLWVIVEKTHKQVLAMKFLGEFKAYVVSGFSTKTRDNPFNEAYNAIDITDISVNLPILPSELNPQSFEEKLRGRSVKNFKFGGDDYFWLIK
SGKTEYL

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
918 bp	305 aa	452	1369	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MIDVNSFNSLFELLEAFPTEQSCIEYLKKVRWGEKVKSPFSPGSKVYVCGNNTYLCKDTGRRFNVKTGTIFENTKMSLRKWFMAVWMVLSNKKGISSLQL
ARDIKVTQKTAWYVLQRIRICLVCENQGKLHNEVEADETFVGGKNKNRHNDKKVKACQGRSFKDKTPVLGMVERKGKVIGIVIKNTSKEELTPKILEFVD
RNAILYTDEWYGYNEIDKMYYHYSIDHSKKQYGYGRINTNTIEGFWTLLKRGYVGIYHYMSSKHLQRYVNEFVFRYNTRKISENSRFNLLLCNMNYHITY
KKLTA

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
263 bp	86 aa	1366	1628	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MSKKEMTIGEEFENFMSFAMRYNFKKKKKVKLYKPTEIAKIYRENGMTEEMLYKRCIGLGCTEEEAKMLVQKCFHPTEKDLELERL

Blast result :

ORF 4

Length		Begin	End	Strand	Fusion ORF
186 bp	61 aa	1640	1825	-	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MENKKCNHVWEEHNAYFKCIHCGELKIKEEIIGTPGIMYEDSYLKDYEEDLKNENKEKESK

Blast result :

ORF 5

Length		Begin	End	Strand	Fusion ORF
597 bp	198 aa	2047	2643	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MTHFLLGTAFIGHVKAEIRIVMKKFVLLILFVFSCTLSSYAQFDNGLQFRDSLAGWTARHAKISEIEPILKKYNIDSSGKRMIIKIDENNIFRIRCNVDS
QDEIEWISMTTLYDSTDSGMKADIMVMTTLLENSKYLGLKFKEEAYFQMSSENNMFFVSEKLGITVVLTTCSMSRRYGCPTLEISFIKGTDYKKWIKQ

Blast result :

Comments

ISBun1 is 60% aa similar to ISRhba1. The second ORF is the transposase, the others are passengers genes.

References

1] Fulton,L., Clifton,S., Fulton,B., Xu,J., Minx,P., Mardis,E.R. and Wilson,R.K. (2007) Direct submission GenBank.