ISBce1
- Family IS1182
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_003909 | ND | Bacillus cereus | Bacillus cereus ATCC 10987 |
DNA section
IS Length : 1803 bp
Ends
IR Length : 16
IRL : GAGGTTGTCCCAAAAGTAGCTGAATAGCTACTTTTGGGCAACCTCTTTCT
IRR : GAGGTTGTCCCAAAAGGTCGTTAAAAACGACCTTTTGGAGACAATTTCTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GCATAAAAAGAAC | GTTCTTTTTACGCA | 0 |
DNA sequence
GAGGTTGTCCCAAAAGTAGCTGAATAGCTACTTTTGGGCAACCTCTTTCTTTACGGAAAATATAAAAAAGAATCGTCCATTTTTGATATAATTAAGTCAC
CACAACCAAATCATCAAAAGAAAGGAACGATTCTTTTGTGTTTATAGATTATAACAAAAACCAACTGACTTTTCCACTAGATGTTGAGGTTCATATTCCT
GAACATCATCTTTCACGTGTGGTTCATACGGCTGTAGAAGCGATGGATCTCTCTATCCTCTTATCACGTTATCCAGGTGGTGGCAGACCACCTTATCACC
CAAAAATGATGTTGAAAATTTTATTATATGCCTACGCAAAAGGAATCTATTCTTCTCGAAAAATTGAAGAACAACTCCATGAAAATATCCATTTCATGTG
GCTTTCTGATGAAAAAACACCAGACTTTCGTACGATCAATCGTTTTCGATCGGAGCGCATGAAAGACGTGATTTATGAAACTTTCTTCTCTCTCATCGAC
CTGTTGAAACAAGAAGGGCTTGTCAAACTCGAAGATTATTTTCTAGATGGAACAAAAATCGAGGCAAATGCCAATCGATATACGTTTGTATGGAAAAAAT
CCACAGAAAAATATGATCAAAAACTAGATGAGAAGTACCAAAAAATTGTCGCGACAATTGAACAAGCAAGTGAAGACGATCAGAAAGCGGAATTAGAAGA
AGAACAAATGAAAGATACCTGTTCGGTCACTTCAGAAAAAATTAAACAAACCATTCAAACCTTAGAAAAACGCCTACAAGATGAACCGAAAAATAAAACG
ATGAAAAAAGCAAAGCGTCAGTTAGAAAATGATCTTCTTCCGCGCAAAATAAAATACGAAGCTCATAAAGAAACATTTCAAGAAAGAAATAGCTTTTCGA
AAACAGATACAGATGCGACCTTTATGCGGATGAAAGAAGATCACATGAAAAATGGTCAACTAAAACCAGGGTACAATATACAAATCGGAACCGAGGATCA
ATTTATTACAGGGTTTAGTATTCATCAAAAAGCGGGAGATACAACCTGTTTGATTCCTCATTTTGAAATATTTGAGAAGTACAATCGTGTAAAGCCTCAA
AATGTAGTTGCCGATTCTGGTTATGGAAGTGAAGAAAACTATGCATTTTGTGAAGAGAAAAAAATGAAAGCATATGTGAAATACAATACGTTTGAAAAAG
AACAAAAGAAGGCGTGGACGAAACAAATAGGTCGCGTGGAAAATATGTTTTATGAGGAAGAACTAGATGAATGGATTTGTGCAAATAAAAAACGCCTTAT
TTTTTCACATGAAAGCAAACAACAAACAAGCAATGGTTATACTTCTATCAAAAGAACCTATCGTTGTACAGAATGTTTAGGCTGTCCATTTCAAGAAACA
TGTGCCAAAGGAAAAGAAACAAAATCTATCAGTGTCTCTGTCAAAAATCAAAAACAGCGAAAAGAAGTACGCGAACGTTTACATACAAAAGAAGGTAAAG
AATTATATGGAAAGCGAAAAATAGAGGTGGAACCCGTATTTGGTCATATTAAATACAATCGTTTCTTCCAACGTTTTTCCTTACGAGGCCTTTCCAAAAA
TCGACTGGAATGGGGTCTTATTTGTGTTGCCCATAATTTACGGAAATGGACAACAACAGCTCAATCAAAAGCGAAAAAACCCCAATCATAAGTAGAAAAA
CAATGGGTTTTCTATTTATGATTGGGGTTTTTATCCATATAAAGGTGAAATATAAGAAATTGTCTCCAAAAGGTCGTTTTTAACGACCTTTTGGGACAAC
CTC
CACAACCAAATCATCAAAAGAAAGGAACGATTCTTTTGTGTTTATAGATTATAACAAAAACCAACTGACTTTTCCACTAGATGTTGAGGTTCATATTCCT
GAACATCATCTTTCACGTGTGGTTCATACGGCTGTAGAAGCGATGGATCTCTCTATCCTCTTATCACGTTATCCAGGTGGTGGCAGACCACCTTATCACC
CAAAAATGATGTTGAAAATTTTATTATATGCCTACGCAAAAGGAATCTATTCTTCTCGAAAAATTGAAGAACAACTCCATGAAAATATCCATTTCATGTG
GCTTTCTGATGAAAAAACACCAGACTTTCGTACGATCAATCGTTTTCGATCGGAGCGCATGAAAGACGTGATTTATGAAACTTTCTTCTCTCTCATCGAC
CTGTTGAAACAAGAAGGGCTTGTCAAACTCGAAGATTATTTTCTAGATGGAACAAAAATCGAGGCAAATGCCAATCGATATACGTTTGTATGGAAAAAAT
CCACAGAAAAATATGATCAAAAACTAGATGAGAAGTACCAAAAAATTGTCGCGACAATTGAACAAGCAAGTGAAGACGATCAGAAAGCGGAATTAGAAGA
AGAACAAATGAAAGATACCTGTTCGGTCACTTCAGAAAAAATTAAACAAACCATTCAAACCTTAGAAAAACGCCTACAAGATGAACCGAAAAATAAAACG
ATGAAAAAAGCAAAGCGTCAGTTAGAAAATGATCTTCTTCCGCGCAAAATAAAATACGAAGCTCATAAAGAAACATTTCAAGAAAGAAATAGCTTTTCGA
AAACAGATACAGATGCGACCTTTATGCGGATGAAAGAAGATCACATGAAAAATGGTCAACTAAAACCAGGGTACAATATACAAATCGGAACCGAGGATCA
ATTTATTACAGGGTTTAGTATTCATCAAAAAGCGGGAGATACAACCTGTTTGATTCCTCATTTTGAAATATTTGAGAAGTACAATCGTGTAAAGCCTCAA
AATGTAGTTGCCGATTCTGGTTATGGAAGTGAAGAAAACTATGCATTTTGTGAAGAGAAAAAAATGAAAGCATATGTGAAATACAATACGTTTGAAAAAG
AACAAAAGAAGGCGTGGACGAAACAAATAGGTCGCGTGGAAAATATGTTTTATGAGGAAGAACTAGATGAATGGATTTGTGCAAATAAAAAACGCCTTAT
TTTTTCACATGAAAGCAAACAACAAACAAGCAATGGTTATACTTCTATCAAAAGAACCTATCGTTGTACAGAATGTTTAGGCTGTCCATTTCAAGAAACA
TGTGCCAAAGGAAAAGAAACAAAATCTATCAGTGTCTCTGTCAAAAATCAAAAACAGCGAAAAGAAGTACGCGAACGTTTACATACAAAAGAAGGTAAAG
AATTATATGGAAAGCGAAAAATAGAGGTGGAACCCGTATTTGGTCATATTAAATACAATCGTTTCTTCCAACGTTTTTCCTTACGAGGCCTTTCCAAAAA
TCGACTGGAATGGGGTCTTATTTGTGTTGCCCATAATTTACGGAAATGGACAACAACAGCTCAATCAAAAGCGAAAAAACCCCAATCATAAGTAGAAAAA
CAATGGGTTTTCTATTTATGATTGGGGTTTTTATCCATATAAAGGTGAAATATAAGAAATTGTCTCCAAAAGGTCGTTTTTAACGACCTTTTGGGACAAC
CTC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1554 bp | 517 aa | 138 | 1691 | + | No |
Chemistry : DDE
ORF sequence :
MFIDYNKNQLTFPLDVEVHIPEHHLSRVVHTAVEAMDLSILLSRYPGGGRPPYHPKMMLKILLYAYAKGIYSSRKIEEQLHENIHFMWLSDEKTPDFRTI
NRFRSERMKDVIYETFFSLIDLLKQEGLVKLEDYFLDGTKIEANANRYTFVWKKSTEKYDQKLDEKYQKIVATIEQASEDDQKAELEEEQMKDTCSVTSE
KIKQTIQTLEKRLQDEPKNKTMKKAKRQLENDLLPRKIKYEAHKETFQERNSFSKTDTDATFMRMKEDHMKNGQLKPGYNIQIGTEDQFITGFSIHQKAG
DTTCLIPHFEIFEKYNRVKPQNVVADSGYGSEENYAFCEEKKMKAYVKYNTFEKEQKKAWTKQIGRVENMFYEEELDEWICANKKRLIFSHESKQQTSNG
YTSIKRTYRCTECLGCPFQETCAKGKETKSISVSVKNQKQRKEVRERLHTKEGKELYGKRKIEVEPVFGHIKYNRFFQRFSLRGLSKNRLEWGLICVAHN
LRKWTTTAQSKAKKPQS
NRFRSERMKDVIYETFFSLIDLLKQEGLVKLEDYFLDGTKIEANANRYTFVWKKSTEKYDQKLDEKYQKIVATIEQASEDDQKAELEEEQMKDTCSVTSE
KIKQTIQTLEKRLQDEPKNKTMKKAKRQLENDLLPRKIKYEAHKETFQERNSFSKTDTDATFMRMKEDHMKNGQLKPGYNIQIGTEDQFITGFSIHQKAG
DTTCLIPHFEIFEKYNRVKPQNVVADSGYGSEENYAFCEEKKMKAYVKYNTFEKEQKKAWTKQIGRVENMFYEEELDEWICANKKRLIFSHESKQQTSNG
YTSIKRTYRCTECLGCPFQETCAKGKETKSISVSVKNQKQRKEVRERLHTKEGKELYGKRKIEVEPVFGHIKYNRFFQRFSLRGLSKNRLEWGLICVAHN
LRKWTTTAQSKAKKPQS
Blast result :
Comments
ISBce1 is 61% aa simialr to IS660. There are one full copy and one partial copy in Bacillus cereus ATCC 10987 genome at coordinates : 2342893-2344695 and 1082855(1)-1082808(48).
References
1] ISfinder annotation (2005)
2] Rasko,D.A., Ravel,J., Okstad,O.A., Helgason,E., Cer,R.Z., Jiang,L., Shores,K.A., Fouts,D.E., Tourasse,N.J., Angiuoli,S.V., Kolonay,J., Nelson,W.C., Kolsto,A.-B., Fraser,C.M. and Read,T.D. (2004) Nucleic Acids Res. 32 (3), 977-988
2] Rasko,D.A., Ravel,J., Okstad,O.A., Helgason,E., Cer,R.Z., Jiang,L., Shores,K.A., Fouts,D.E., Tourasse,N.J., Angiuoli,S.V., Kolonay,J., Nelson,W.C., Kolsto,A.-B., Fraser,C.M. and Read,T.D. (2004) Nucleic Acids Res. 32 (3), 977-988