ISAzvi12

  • Family IS110
  • Group IS1111
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP001157 ND Azotobacter vinelandii
Azotobacter vinelandii DJ
DNA section
IS Length : 1247 bp

Ends


IR Length : 12

IRL : tgctgtgATGGACTCTCCCTGCACCACACTTATCCGCACCAACGGTGCCG
IRR : ----tatATGGACTCTCCCCACAAGTGGTGAGTAAAGCCTTTCCAACCCT

Insertion site


Left flankDirect repeatRight flankDR Length
TCGGCTTGAATGAATTGTTA0

DNA sequence

TGCTGTGATGGACTCTCCCTGCACCACACTTATCCGCACCAACGGTGCCGTGAGTGATGTTCGGAGGATTGCAGCGATGCCCAAACAGATTGCAGTGGAT
TTGGCCAAGTCCGTTTACCAGGTTGCCGAGAGCGTCCATGCCGGCCACGTGAATCAGCGCAGACGGCTGGGTCGCGAGGCATTTCGCCGGTATATACAGG
AGCAGGCGGAACCGGTCGAGTGGCTGATGGAAGCCTGTGGCACGGCGCACTACTGGGGACGCTTCGCCCAGTCGCTGGGGCATCGGGTGATCCTGCTGCA
TCCGCGCTACGTGAAACCCTACCGGCGACGCAACAAGACCGACCGCAACGACTGCGACGCCCTCCTCGAAGCGGCGCGCTGCGCCGGCATTCATCCGGTG
CCGGTGAAGAGCCATGAGCAGCAGCAACTGCAACAGCTGCACGGCCTGCGCGAAGCTTGGAAGAAGAGCCGCACCCAGCGCATCAACCTGCTGCGCGGCA
TCTTCCGCGAGATGGGCCTTGAGGCGCCGGCGTCGATAGCTGCCTTCCTCCATGCAGCCAATGAACTGGTTGAGCAACCCGAAGTGGCGGCCCGGCGCGG
CCAACTGCAGATCGTCCTGGCGGAGATCAACCTGTACGAGCAATGCATGGTCGAGTGCGAGCAGCAGCTCGCCCGCTGGCATGCCGACGACGCCATCGTG
CGCAGGCTCGACGAAGTCAGCGGCATCGGTCTGCTGAGTGCCAGCGCCCTGAAAACGGCGGTCGGCCAGCCCGAGCGCTTTGCCAGTGGCCGCCAGTTGA
GCGCCTGGCTGGGCATGACGCCCCGCGAGTACAGCAGTGGCAATACCCGCAAGCTGGAACACATCAGCCGGCAGGGCAACGTCTATGTGCGCACCCTGCT
GATCCACGGCGCCGCCAGCAAGGCGGCCGTGGCGCTGGCCAACAAGCTGGTGCGGATCTGCTGGGCGATGTGGTGTCATGAGCGGCGCTTCAGCGGCGAC
TGGCAGAGCAGCAGGTTTTCCCGTGGTTGTGGTGAATAGCGACACTGTCGGAACAGGCGAGTCCTGCAGCGGAGCAAGGCCGATAACAATGCTGATCCTG
GTGATCGATTGAACGCTTGGCCCCCGCTGCGCGAACCACAGAGTGGCCCGGACGGTAATGTCCATGGAAGGCCGGATATATGAATGCAACCGCACTGGCG
ACAGGGTTGGAAAGGCTTTACTCACCACTTGTGGGGAGAGTCCATATA
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
963 bp320 aa771039+No
ORF function : Transposase
Chemistry : DEDD

ORF sequence :

MPKQIAVDLAKSVYQVAESVHAGHVNQRRRLGREAFRRYIQEQAEPVEWLMEACGTAHYWGRFAQSLGHRVILLHPRYVKPYRRRNKTDRNDCDALLEAA
RCAGIHPVPVKSHEQQQLQQLHGLREAWKKSRTQRINLLRGIFREMGLEAPASIAAFLHAANELVEQPEVAARRGQLQIVLAEINLYEQCMVECEQQLAR
WHADDAIVRRLDEVSGIGLLSASALKTAVGQPERFASGRQLSAWLGMTPREYSSGNTRKLEHISRQGNVYVRTLLIHGAASKAAVALANKLVRICWAMWC
HERRFSGDWQSSRFSRGCGE

 

Blast result :
Comments
The IR of ISAzvi12 are not at its termini. In the IS sequence, as given, 7nt separate IRl from the left-hand end of the element and 3nt separates IRr from the right-hand end. Like other IS1111 family, ISKpn4 subgroup members, ISAzvi12 targets a specific site in the 59be (or attC sites) in gene cassettes associated with integrons and is inserted in only one orientation.
ISAzvi12 is 79% aa similar to ISKpn4.
References
1] Partridge and Hall (2003) J.Bacteriol. 185, 6371-6384
2] Post, V. and Hall, R.M. (2007) unpublished