IS982B

  • Family IS982
  • Group
MGE type ISRelated element(s) :
Isoform IS982Synonym(s) IS982
Accession numberTranspositionOriginHost
S77101 ND Lactococcus lactis
Streptococcus thermophilus Sfi39
Lactococcus lactis biovar diacetylactis CRL264 plasmid pCIT264
DNA section
IS Length : 999 bp

Ends


IR Length : 19/20

IRL : ACCCGAATTGCTAGTTAATTTCATTGGAAAATAAATAAGTCGTGCTATCC
IRR : ACCCGAATTGCTAGTTGATTATTTAGCCATGACTTGATACCCGATAGAAT

Insertion site


Left flankDirect repeatRight flankDR Length

DNA sequence

ACCCGAATTGCTAGTTAATTTCATTGGAAAATAAATAAGTCGTGCTATCCTAATCTTAAAGCACTAAGCATTAGAAAGCTCACGACTTATATGACTTATA
ATAGCACACTTCCAAAAGTTTTTGTTTATTTACTGACAACCATTGAGACGCTTTATCAAACGAGTGTTCCCCTTGAGGTTCAAAACCGAAAGAACGTCCA
TCTTGCAACATCTGACTGTTTAGTTATCGCTTGTTACCTATGGGGCGTACTGCATTTTAGTGAAACGCTTAAAGCTAAGCACCAATTGGCTCAAAGTTTA
TTTCCTAATTTCCTAGAATATTCTCGCTTTGTCCGCCGTTGTAATGCCCTCTTACCGAGTATCCAAGTCATTCGCCAAGCACTCGTCTTTAAAGAGGTTG
AAGGAATGAGTGTATCCATTATTGACAGCTTCCCCATTCCTTTGTGTCAGCCTATTCGTAATTTCAGAAGCAAAGTTCTTGGAGATTATGCAAATGTTGG
CTACAATGCTACAAAGGGACAGTACTTCTATGGCTGTAAATGTCATGCTTTAGTCAGTGAATCAGGCTATGTCATAGACTACACAATTACTCCTGCTTCA
ATGGCTGATCGTTCAATGACCGAGGAAGTGTTGAGTCAATTTGGGACACCAACAGTCCTTGGAGATATGGGATATCTAGGTCAGTCACTGCATGATAGGC
TGGAATTAAAAGGAATTGATCTAATGACACCTGTCAGGAAGAACATGAAGCAAAAGAAAATCCTTTTCCCTAATTTTTCAAAACGTAGAAAAGTGATTGA
GCGAGTTTTCTCTTTTTTGACAAATCTAGGAGCTGAGCGTTGTAAAAGTCGTTCGCCTCAAGGTTTTCAATTGAAATTAGAGATGATACTTTTAGCGTAT
TCTTTACTGTTAAAATCAGCTAAATCACTGGAACCAGAGACTTTAAGATATTCTATCGGGTATCAAGTCATGGCTAAATAATCAACTAGCAATTCGGGT
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
891 bp296 aa91981+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTYNSTLPKVFVYLLTTIETLYQTSVPLEVQNRKNVHLATSDCLVIACYLWGVLHFSETLKAKHQLAQSLFPNFLEYSRFVRRCNALLPSIQVIRQALVF
KEVEGMSVSIIDSFPIPLCQPIRNFRSKVLGDYANVGYNATKGQYFYGCKCHALVSESGYVIDYTITPASMADRSMTEEVLSQFGTPTVLGDMGYLGQSL
HDRLELKGIDLMTPVRKNMKQKKILFPNFSKRRKVIERVFSFLTNLGAERCKSRSPQGFQLKLEMILLAYSLLLKSAKSLEPETLRYSIGYQVMAK

 

Blast result :
Comments
The pCIT264 citrate plasmid from Lactococcus lactis biovar diacetylactis contains IS982B which is located between the citQRP cluster and its transcriptional promoter (Lopez de Felipe et al., 1995). The insertion of IS982B has created a new hybrid promoter (the -35 box located 12 bp from the right end of the element) that drives the transcription of the citQRP cluster during exponential and stationary phases of growth of Lactococcus lactis (Lopez de Felipe et al., 1996). At least six copies of IS982B are located in the chromosome of strain CRL264 of Lactococcus lactis biovar diacetylactis (Magni et al., 1996). IS982B transposase is 97.3 % identical to that of IS982.
IS982 is also present in Streptococcus thermophilus (Accesion number : AF373595 ), this sequence is 98% DNA and aa similar to the sequence of the database. There is a codon stop at position 144 instead of Q, so the transposase is inactive.
References
1] Lopez de Felipe, F., Magni, C., de Mendoza, D., and Lopez, P. (1995) Mol. Gen. Genet. 246, 590-599.
2] Lopez de Felipe, F., Magni, C., de Mendoza, D., and Lopez, P. (1996) Mol. Gen. Genet. 250, 428-436.
3] Magni, C., Lopez de Felipe, F., Lopez, P., and de Mendoza, D. (1996) FEMS Microbiol. Lett. 136, 289-295.
4] Germond,J.-E. and Delley,M. (2001) Direct submission GenBank
5] Germond,J.E., Delley,M., D'Amico,N. and Vincent,S.J. (2001) Eur. J. Biochem. 268 (19), 5149-5156