IS942
- Family IS1380
- Group IS942
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| J03326 | Y | Bacteroides fragilis | Bacteroides fragilis TAL3636 |
DNA section
IS Length : 1598 bp
Ends
IR Length : 13/15
IRL : CCTTAATTCCGCAACACTATAGTTTAACATTATTTATAAGTGCCTGAGCA
IRR : CCTCAAATCCGCAACCTATAGGGTTTAGTGGGATTTTGCTTGCAAAGCGA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TTATTCTTTT | ATATGCTG | TATTCAAACT | 8 |
DNA sequence
CCTTAATTCCGCAACACTATAGTTTAACATTATTTATAAGTGCCTGAGCAACAAAAAGTTGCCCAGGATTTTGCCATGTCAGAATTTTCACTTACCTTAG
TGTTGCGAAAAGAAGACAAGCAAAACTCTAATATGACATGGCAAAAGTACAAATAAAATCTGAGAAACTCACTCCTTTTGGAGGAATTTTTTCTATTATG
GAGCAATTTGATGCTCTTTTAGCTCAAACCATAGATTCCACCTTGGGATTGAGATGCACTATGTTTGGTTATCAATATAGCGAAATTCTACGCTCTCTGA
TGTGCGTATATCTTTGTGGCGGCTCATGTATTGAGGATGTTACAACTCACCTGATGAAACATTTGTCTCTTCATCCAACTCTTCGCACTTGCAGCGCAGA
CACCATATTGCGTGCTATCGAAGAACTGACTTGTAAGAACATCACCTATAAATCTGCTTCTGCTTCTGGCAACTCCTATGATTTCAATACTGCAGACAAG
ATGAACTGCTTATTGATCAAAGCCCTGCTTGCTACTGGTCAATTGAAATCCGGTCAAGAGTATGATTTTGACTTTGACCATCAGTTCATTGAAACAGAGA
AGTATGATGCAAAACCAACCTACAAGAAGTTCTTGGGCTATAGTCCAGGCGTAGCTGTCATTAACGACATGATTGTTGGTATTGAAAATAGAGACGGCAA
CACAAACGTACGCTTCAACCAAAAAGAAACTTTGGAAAGAATCTTCAAGCGATTGGAGGCTTCGGAAATATATATTTCTCGTGCCCGCATGGATTGCGGC
TCATGTTCGGAGGAAATCGTAGATATGGTAGAGGCTCATTGCAGGCATTTTTATATTCGTGCCAACAGATGCTCTTCTTTCTACGATTCCATGTTTGCCT
TAACTGGATGGAAAACTGTTGAAATCAACGGTATTGAGTTTGAGTTGAATTCTATCCTTGTTGAGAAATGGAAAGGAAAACCGTATCGTCTTGTCATACA
GAGACAAAGGCGAATAGATGGAGACCTTGACATTTGGGAAGGCGAATATACCTACAGATGTATACTGACTAACGATTACAAGTCGAGTGCAAGAGACATC
GTGGAATTCTACAATCTTCGTGGTGGCAAGGAACGCATCTTCGATGACATGAACAATGACTTTGGCTGGAATCGATTGCCAAAATCGTTCATGGCACAGA
ATACTGTATTCCTGCTTATGACAGCTCTCATCAGAAACTTCTACAAAGCTATTATGCAGAGATTGAAAACCCATGAATTTGGATTGCGTGCCACCAGCAG
AATCAAGACCTTTGTTTTCAAGTTCATCTCTGTTCCTGCGAAATGGATTAAGACATCACGTAGGCATGTATTGAACATTTACTCAGACAACAATGCTTAT
GCCAACCTGTTCAAGACAGACTTTGGTTAAAGACCATGCTTTTCTGGTTAAACCAGCGTATTACCTCAAGTCGCTTTATGGGGTAAGGGGATTTTGTGTC
TGCGACATTTCTGTTGTGCAAGAAATATGTACAATAAAATGAATTTTGTCGCTTTGCAAGCAAAATCCCACTAAACCCTATAGGTTGCGGATTTGAGG
TGTTGCGAAAAGAAGACAAGCAAAACTCTAATATGACATGGCAAAAGTACAAATAAAATCTGAGAAACTCACTCCTTTTGGAGGAATTTTTTCTATTATG
GAGCAATTTGATGCTCTTTTAGCTCAAACCATAGATTCCACCTTGGGATTGAGATGCACTATGTTTGGTTATCAATATAGCGAAATTCTACGCTCTCTGA
TGTGCGTATATCTTTGTGGCGGCTCATGTATTGAGGATGTTACAACTCACCTGATGAAACATTTGTCTCTTCATCCAACTCTTCGCACTTGCAGCGCAGA
CACCATATTGCGTGCTATCGAAGAACTGACTTGTAAGAACATCACCTATAAATCTGCTTCTGCTTCTGGCAACTCCTATGATTTCAATACTGCAGACAAG
ATGAACTGCTTATTGATCAAAGCCCTGCTTGCTACTGGTCAATTGAAATCCGGTCAAGAGTATGATTTTGACTTTGACCATCAGTTCATTGAAACAGAGA
AGTATGATGCAAAACCAACCTACAAGAAGTTCTTGGGCTATAGTCCAGGCGTAGCTGTCATTAACGACATGATTGTTGGTATTGAAAATAGAGACGGCAA
CACAAACGTACGCTTCAACCAAAAAGAAACTTTGGAAAGAATCTTCAAGCGATTGGAGGCTTCGGAAATATATATTTCTCGTGCCCGCATGGATTGCGGC
TCATGTTCGGAGGAAATCGTAGATATGGTAGAGGCTCATTGCAGGCATTTTTATATTCGTGCCAACAGATGCTCTTCTTTCTACGATTCCATGTTTGCCT
TAACTGGATGGAAAACTGTTGAAATCAACGGTATTGAGTTTGAGTTGAATTCTATCCTTGTTGAGAAATGGAAAGGAAAACCGTATCGTCTTGTCATACA
GAGACAAAGGCGAATAGATGGAGACCTTGACATTTGGGAAGGCGAATATACCTACAGATGTATACTGACTAACGATTACAAGTCGAGTGCAAGAGACATC
GTGGAATTCTACAATCTTCGTGGTGGCAAGGAACGCATCTTCGATGACATGAACAATGACTTTGGCTGGAATCGATTGCCAAAATCGTTCATGGCACAGA
ATACTGTATTCCTGCTTATGACAGCTCTCATCAGAAACTTCTACAAAGCTATTATGCAGAGATTGAAAACCCATGAATTTGGATTGCGTGCCACCAGCAG
AATCAAGACCTTTGTTTTCAAGTTCATCTCTGTTCCTGCGAAATGGATTAAGACATCACGTAGGCATGTATTGAACATTTACTCAGACAACAATGCTTAT
GCCAACCTGTTCAAGACAGACTTTGGTTAAAGACCATGCTTTTCTGGTTAAACCAGCGTATTACCTCAAGTCGCTTTATGGGGTAAGGGGATTTTGTGTC
TGCGACATTTCTGTTGTGCAAGAAATATGTACAATAAAATGAATTTTGTCGCTTTGCAAGCAAAATCCCACTAAACCCTATAGGTTGCGGATTTGAGG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1293 bp | 430 aa | 138 | 1430 | + | No |
Chemistry : DDE
ORF sequence :
MAKVQIKSEKLTPFGGIFSIMEQFDALLAQTIDSTLGLRCTMFGYQYSEILRSLMCVYLCGGSCIEDVTTHLMKHLSLHPTLRTCSADTILRAIEELTCK
NITYKSASASGNSYDFNTADKMNCLLIKALLATGQLKSGQEYDFDFDHQFIETEKYDAKPTYKKFLGYSPGVAVINDMIVGIENRDGNTNVRFNQKETLE
RIFKRLEASEIYISRARMDCGSCSEEIVDMVEAHCRHFYIRANRCSSFYDSMFALTGWKTVEINGIEFELNSILVEKWKGKPYRLVIQRQRRIDGDLDIW
EGEYTYRCILTNDYKSSARDIVEFYNLRGGKERIFDDMNNDFGWNRLPKSFMAQNTVFLLMTALIRNFYKAIMQRLKTHEFGLRATSRIKTFVFKFISVP
AKWIKTSRRHVLNIYSDNNAYANLFKTDFG
NITYKSASASGNSYDFNTADKMNCLLIKALLATGQLKSGQEYDFDFDHQFIETEKYDAKPTYKKFLGYSPGVAVINDMIVGIENRDGNTNVRFNQKETLE
RIFKRLEASEIYISRARMDCGSCSEEIVDMVEAHCRHFYIRANRCSSFYDSMFALTGWKTVEINGIEFELNSILVEKWKGKPYRLVIQRQRRIDGDLDIW
EGEYTYRCILTNDYKSSARDIVEFYNLRGGKERIFDDMNNDFGWNRLPKSFMAQNTVFLLMTALIRNFYKAIMQRLKTHEFGLRATSRIKTFVFKFISVP
AKWIKTSRRHVLNIYSDNNAYANLFKTDFG
Blast result :
Comments
In Bacteroides fragilis TAL3636, IS942 is integrated upstream of the metallo-beta-lactamase gene ccrA (Rasmussen and Kovacs, 1991). DNA sequences very similar to IS942 are present within the 5' untranslated region of the Bacteroides fragilis metallo-beta-lactamase cfiA gene (Thompson and Malamy, 1990). IS942 and IS1170 (from Bacteroides thetaiotaomicron BT13 (pIP419)) putative transposases are 76 % identical (426-aa overlap).
References
1] Thompson, J.S., and Malamy, M.H. (1990) J. Bacteriol. 172, 2584-2593.
2] Rasmussen, B.A., and Kovacs, E. (1991) Plasmid 25, 141-144.
2] Rasmussen, B.A., and Kovacs, E. (1991) Plasmid 25, 141-144.