IS911
- Family IS3
- Group IS3
Isoform Synonym(s) ISSHO1
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| X17613 | ND | Shigella dysenteriae | Escherichia coli K12 Shigella sonnei OFB759 Shigella dysenteriae ATCC11456 Shigella boydii OFB760 Shigella dysenteriae OFB762 Shigella flexneri OFB761 |
DNA section
IS Length : 1250 bp
Ends
IR Length : 25/36
IRL : TGAAGTGGCACACTGAATTTGGCCACCTGAACAGAGGTGATATGCTCACC
IRR : TGAAGTGGTCAACAAAAACTGGCCACCGAGTTAGAGTTTTTTCCAGTATC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| NNNNNNCTTC | GTG | CGGTNNNNNN | 3 |
| NNNNNNTTAT | CTG | TCACNNNNNN | 3 |
| NNNNNNCTGG | CTC | TGAANNNNNN | 3 |
| NNNNNNGAGT | ATC | TGGTNNNNNN | 3 |
| NNNNNNGCCT | GGA | GAATNNNNNN | 3 |
| NNNNNNAGTC | GAG | CGACNNNNNN | 3 |
| NNNNNNNNCG | CCT | CCNNNNNNNN | 3 |
| NNNNNNNNAT | GTT | TGNNNNNNNN | 3 |
| NNNNNNNNGG | AAG | CCNNNNNNNN | 3 |
| NNNNNNNNAT | CTG | TGNNNNNNNN | 3 |
| NNNNNNNNTT | ATTG | TGNNNNNNN | 4 |
| NNNNNNNNAA | ATGG | AANNNNNNN | 4 |
DNA sequence
TGAAGTGGCACACTGAATTTGGCCACCTGAACAGAGGTGATATGCTCACCTCAGAACAACACAGGTGCTCCAATGAAAAAAAGAAATTTCAGCGCAGAGT
TTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAACTACACGGTGGCAGATGCCGCCAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATGGGT
CAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTGAGCTGAGGAAAAAGCTACAACGC
ATTGAAATGGAGAATGAAATATTAAAAAAGGCTACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCC
TGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGC
AGTCAGGTACTTGAGCTACATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGGACGCTGGC
TTGCTGGCAGGCTCATGAAAGAGCTGGGGCTGGTCAGCTGTCAGCAGCCGACTCACCGGTATAAACGTGGTGGTCATGAACATGTTGCTATCCCTAACTA
CCTTGAACGGCAGTTCGCCGTGACCGAGCCAAATCAGGTGTGGTGCGGTGATGTGACCTATATCTGGACGGGTAAGCGCTGGGCGTACCTCGCCGTTGTT
CTCGACCTGTTCGCAAGAAAACCAGTGGGCTGGGCCATGTCGTTCTCGCCGGACAGCAGGCTCACCATGAAAGCGCTGGAAATGGCATGGGAAACCCGTG
GTAAGCCCGGCGGGGTGATGTTCCACAGCGATCAGGGCAGTCATTATACGAGCAGGCAGTTCCGGCAGTTATTGTGGCGATACCAGATCAGGCAGAGTAT
GAGCCGGCGCGGAAATTGCTGGGATAACAGCCCAATGGAACGCTTCTTCAGAAGTCTGAAGAACGAATGGATACCGGTGGTGGGTTACGTAAGCTTCAGC
GAGGCAGCTCACGCCATAACGGATTATATCGTTGGATATTACAGCGCACTAAGACCGCACGAATATAACGGTGGGTTACCCCCAAACGAATCGGAAAATC
GATACTGGAAAAAACTCTAACTCGGTGGCCAGTTTTTGTTGACCACTTCA
TTAAACGCGAATCCGCTCAACTGGTTGTTGACCAGAACTACACGGTGGCAGATGCCGCCAAAGCTATGGATGTTGGCCTTTCCACAATGACAAGATGGGT
CAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTGAGCTGAGGAAAAAGCTACAACGC
ATTGAAATGGAGAATGAAATATTAAAAAAGGCTACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCC
TGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGC
AGTCAGGTACTTGAGCTACATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGGACGCTGGC
TTGCTGGCAGGCTCATGAAAGAGCTGGGGCTGGTCAGCTGTCAGCAGCCGACTCACCGGTATAAACGTGGTGGTCATGAACATGTTGCTATCCCTAACTA
CCTTGAACGGCAGTTCGCCGTGACCGAGCCAAATCAGGTGTGGTGCGGTGATGTGACCTATATCTGGACGGGTAAGCGCTGGGCGTACCTCGCCGTTGTT
CTCGACCTGTTCGCAAGAAAACCAGTGGGCTGGGCCATGTCGTTCTCGCCGGACAGCAGGCTCACCATGAAAGCGCTGGAAATGGCATGGGAAACCCGTG
GTAAGCCCGGCGGGGTGATGTTCCACAGCGATCAGGGCAGTCATTATACGAGCAGGCAGTTCCGGCAGTTATTGTGGCGATACCAGATCAGGCAGAGTAT
GAGCCGGCGCGGAAATTGCTGGGATAACAGCCCAATGGAACGCTTCTTCAGAAGTCTGAAGAACGAATGGATACCGGTGGTGGGTTACGTAAGCTTCAGC
GAGGCAGCTCACGCCATAACGGATTATATCGTTGGATATTACAGCGCACTAAGACCGCACGAATATAACGGTGGGTTACCCCCAAACGAATCGGAAAATC
GATACTGGAAAAAACTCTAACTCGGTGGCCAGTTTTTGTTGACCACTTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 303 bp | 100 aa | 73 | 375 | + | No |
Description : First part of the transposase
ORF sequence :
MKKRNFSAEFKRESAQLVVDQNYTVADAAKAMDVGLSTMTRWVKQLRDERQGKTPKASPITPEQIEIRELRKKLQRIEMENEILKKATALLMSDSLNSSR
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 819 bp | 273 aa | 402 | 1220 | + | No |
Description : Second part of the transposase
ORF sequence :
NIKKGYRALDVRLPEQFSIIGKLRAHYPVVTLCHVFGVHRSSYRYWKNRPEKPDGRRAVLRSQVLELHGISHGSAGARSIATMATRRGYQMGRWLAGRLM
KELGLVSCQQPTHRYKRGGHEHVAIPNYLERQFAVTEPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTMKALEMAWETRGKPGGV
MFHSDQGSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERFFRSLKNEWIPVVGYVSFSEAAHAITDYIVGYYSALRPHEYNGGLPPNESENRYWKKL
KELGLVSCQQPTHRYKRGGHEHVAIPNYLERQFAVTEPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTMKALEMAWETRGKPGGV
MFHSDQGSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERFFRSLKNEWIPVVGYVSFSEAAHAITDYIVGYYSALRPHEYNGGLPPNESENRYWKKL
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1148 bp | 382 aa | 73 | 1220 | + | Yes |
Chemistry : DDE
ORF sequence :
MKKRNFSAEFKRESAQLVVDQNYTVADAAKAMDVGLSTMTRWVKQLRDERQGKTPKASPITPEQIEIRELRKKLQRIEMENEILKKGYRALDVRLPEQFS
IIGKLRAHYPVVTLCHVFGVHRSSYRYWKNRPEKPDGRRAVLRSQVLELHGISHGSAGARSIATMATRRGYQMGRWLAGRLMKELGLVSCQQPTHRYKRG
GHEHVAIPNYLERQFAVTEPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTMKALEMAWETRGKPGGVMFHSDQGSHYTSRQFRQL
LWRYQIRQSMSRRGNCWDNSPMERFFRSLKNEWIPVVGYVSFSEAAHAITDYIVGYYSALRPHEYNGGLPPNESENRYWKKL
IIGKLRAHYPVVTLCHVFGVHRSSYRYWKNRPEKPDGRRAVLRSQVLELHGISHGSAGARSIATMATRRGYQMGRWLAGRLMKELGLVSCQQPTHRYKRG
GHEHVAIPNYLERQFAVTEPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTMKALEMAWETRGKPGGVMFHSDQGSHYTSRQFRQL
LWRYQIRQSMSRRGNCWDNSPMERFFRSLKNEWIPVVGYVSFSEAAHAITDYIVGYYSALRPHEYNGGLPPNESENRYWKKL
Blast result :
Comments
References