IS903
- Family IS5
- Group IS903
Isoform Synonym(s) IS903L, IS903R, Tn55, Tn601, IS602, Tn60
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| J01839 | Y | Escherichia coli | Escherichia coli plasmid pR6-5 Citrobacter freundii B38, plasmid pOZ172 |
DNA section
IS Length : 1057 bp
Ends
IR Length : 18
IRL : GGCTTTGTTGAATAAATCGAACTTTTGCTGAGTTGAAGGATCAGATCACG
IRR : GGCTTTGTTGAATAAATCAGATTTCGGGTAAGTCTCCCCCGTAGCGGGTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GACTTTTTAT | ATCAATAAA | GAATAATTAA | 9 |
| GATCCTTTAA | TCTTTTCTA | CTGAACCGCG | 9 |
| TCCCGGAGAC | GGTCACAGC | TTGTCTGTGA | 9 |
| GTTTGTTGAA | GGCGATATT | CGTAACGAAG | 9 |
| TGTGCCGCAG | GGTGCCGGG | TTAAGTTCTT | 9 |
| TACCCTGCCA | CTCACACCA | TTCAGGCGCC | 9 |
DNA sequence
GGCTTTGTTGAATAAATCGAACTTTTGCTGAGTTGAAGGATCAGATCACGCATCTTCCCGACAACGCAGACCGTTCCGTGGCAAAGCAAAAGTTCAAAAT
CACCAACTGGTCCACCTACAACAAAGCTCTCATCAACCGTGGCTCCCTCACTTTCTGGCTGGATGATGGGGCGATTCAGGCCTGGTATGAGTCAGCAACA
CCTTCTTCACGAGGCAGACCTCAGCGCTATTCTGACCTTGCCATCACGACTGTGCTGGTCATTAAACGCGTATTCAGGCTGACCCTGCGCGCTGCGCAGG
GCTTTATTGATTCCATTTTTACACTGATGAATGTTCCGTTGCGCTGCCCGGATTACAGCTGTGTCAGCAGGCGGGCAAAGTCGGTTAATATCAGTTTCAA
AACGTTCACCCGGGGTGAAATCGCGCATCTGGTGATTGATTCCACCGGGCTGAAGGTCTTTGGTGAAGGCGAATGGAAAGTCAAAAAGCATGGCCAGGAA
CGCCGCCGTATCTGGCGTAAGCTGCATCTCGCCGTTGACAGTAAAACACATGAAATCATCTGCGCTGACCTGTCGCTGAACAATGTGACCGACTCAGAAG
CCTTCCCGGGTCTTATCCGGCAGACTCACAGAAAAATCAGGGCAGCATCGGCAGACGGCGCTTACGACACCCGGCTCTGTCACGATGAACTGCGGCGTAA
GAAAATCAGCGCGCTTATCCCTCCCCGAAAAGGTGCGGGTTACTGGCCCGGTGAATATGCAGACCGTAACCGTGCAGTGGCTAATCAGCGAATGACCGGG
AGTAATGCGCGGTGGAAATGGACAACAGATTACAACCGTCGCTCGATAGCGGAAACGGCGATGTACCGGGTAAAACAGCTGTTCGGGGGTTCACTGACGC
TGCGTGACTACGATGGTCAGGTTGCGGAGGCTATGGCCCTGGTACGAGCGCTGAACAAAATGACGAAAGCAGGTATGCCTGAAAGCGTGCGTATTGCCTG
AAAACACAACCCGCTACGGGGGAGACTTACCCGAAATCTGATTTATTCAACAAAGCC
CACCAACTGGTCCACCTACAACAAAGCTCTCATCAACCGTGGCTCCCTCACTTTCTGGCTGGATGATGGGGCGATTCAGGCCTGGTATGAGTCAGCAACA
CCTTCTTCACGAGGCAGACCTCAGCGCTATTCTGACCTTGCCATCACGACTGTGCTGGTCATTAAACGCGTATTCAGGCTGACCCTGCGCGCTGCGCAGG
GCTTTATTGATTCCATTTTTACACTGATGAATGTTCCGTTGCGCTGCCCGGATTACAGCTGTGTCAGCAGGCGGGCAAAGTCGGTTAATATCAGTTTCAA
AACGTTCACCCGGGGTGAAATCGCGCATCTGGTGATTGATTCCACCGGGCTGAAGGTCTTTGGTGAAGGCGAATGGAAAGTCAAAAAGCATGGCCAGGAA
CGCCGCCGTATCTGGCGTAAGCTGCATCTCGCCGTTGACAGTAAAACACATGAAATCATCTGCGCTGACCTGTCGCTGAACAATGTGACCGACTCAGAAG
CCTTCCCGGGTCTTATCCGGCAGACTCACAGAAAAATCAGGGCAGCATCGGCAGACGGCGCTTACGACACCCGGCTCTGTCACGATGAACTGCGGCGTAA
GAAAATCAGCGCGCTTATCCCTCCCCGAAAAGGTGCGGGTTACTGGCCCGGTGAATATGCAGACCGTAACCGTGCAGTGGCTAATCAGCGAATGACCGGG
AGTAATGCGCGGTGGAAATGGACAACAGATTACAACCGTCGCTCGATAGCGGAAACGGCGATGTACCGGGTAAAACAGCTGTTCGGGGGTTCACTGACGC
TGCGTGACTACGATGGTCAGGTTGCGGAGGCTATGGCCCTGGTACGAGCGCTGAACAAAATGACGAAAGCAGGTATGCCTGAAAGCGTGCGTATTGCCTG
AAAACACAACCCGCTACGGGGGAGACTTACCCGAAATCTGATTTATTCAACAAAGCC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 924 bp | 307 aa | 78 | 1001 | + | No |
Chemistry : DDE
ORF sequence :
VAKQKFKITNWSTYNKALINRGSLTFWLDDGAIQAWYESATPSSRGRPQRYSDLAITTVLVIKRVFRLTLRAAQGFIDSIFTLMNVPLRCPDYSCVSRRA
KSVNISFKTFTRGEIAHLVIDSTGLKVFGEGEWKVKKHGQERRRIWRKLHLAVDSKTHEIICADLSLNNVTDSEAFPGLIRQTHRKIRAASADGAYDTRL
CHDELRRKKISALIPPRKGAGYWPGEYADRNRAVANQRMTGSNARWKWTTDYNRRSIAETAMYRVKQLFGGSLTLRDYDGQVAEAMALVRALNKMTKAGM
PESVRIA
KSVNISFKTFTRGEIAHLVIDSTGLKVFGEGEWKVKKHGQERRRIWRKLHLAVDSKTHEIICADLSLNNVTDSEAFPGLIRQTHRKIRAASADGAYDTRL
CHDELRRKKISALIPPRKGAGYWPGEYADRNRAVANQRMTGSNARWKWTTDYNRRSIAETAMYRVKQLFGGSLTLRDYDGQVAEAMALVRALNKMTKAGM
PESVRIA
Blast result :
Comments
IS903 promotes both adjacent deletions and deletion-inversions, thought to be the result of an intramolecular replicative process (cointegrate formation). IS903 uses both the conservative and replicative way of transposition, with a net preference for the latter (Bernardi and Bernardi, 1991). The cis preference of the IS903 transposase is mediated by a combination of transposase instability and inefficient translation initiation (Derbyshire and Grindley, 1992 and 1996).
Found in Tn903.
Tn602 is flanked by two iso-IS602 and encodes resistance to kanamycin. IS602L and IS602R are closely related (less than 100 bp different) to IS102, IS903 and IS903B, IS102 being the most divergent. Tn602 was found in Capnocytophaga ochraceus (causes infections in humans)
Found in Tn903.
Tn602 is flanked by two iso-IS602 and encodes resistance to kanamycin. IS602L and IS602R are closely related (less than 100 bp different) to IS102, IS903 and IS903B, IS102 being the most divergent. Tn602 was found in Capnocytophaga ochraceus (causes infections in humans)
References
1] Kopecko, D.J., Brevet, J., and Cohen, S.N. (1976) J. Mol. Biol. 108, 333-360.
2] Nomura, N., Yamagishi, H., and Oka, A. (1978) Gene 3, 39-52.2.
3] Oka, A., Nomura, N., Sugimoto, K., Sugisaki, H., and Takanami, M. (1978) Nature 276, 845-847.
4] Grindley, N.D.F., and Joyce, C.M. (1980) Proc. Natl. Acad. Sci., USA 77, 7176- 7180.
5] Oka, A., Sugisaki, H., and Takanami, M. (1981) J. Mol. Biol. 147, 217-226.
6] Weinert, T.A., Schaus, N.A., and Grindley, N.D.F. (1983) Science 222, 755-765.
7] Mollet, B., Clerget, M., Meyer, J., and Iida, S. (1985) J. Bacteriol. 163, 55-60.
8] Mollet, B., Iida, S., and Arber, W. (1985) Mol. Gen. Genet. 199, 534-536.
9] Derbyshire, K.M., Hwang, L., and Grindley, N.D.F. (1987) Proc. Natl. Acad. Sci., USA 84, 8049-8053.
10] Stibitz, S., and Davies, J. (1987) Plasmid 17, 202-209.
11] Derbyshire, K.M., Kramer, M., and Grindley, N.D.F. (1990) Proc. Natl. Acad. Sci., USA 87, 4048-4052.
12] Bernardi, F., and Bernardi, A. (1991) Mol. Gen. Genet. 227, 22-27.
13] Derbyshire, K.M., and Grindley, N.D.F. (1992) EMBO J. 11, 3449-3455.
14] Wiater, L., Sadosky, A.B., and Shuman, H.A. (1994) Mol. Microbiol. 11, 641-653.
15] Derbyshire, K.M., and Grindley, N.D.F. (1996) Mol. Microbiol. 21, 1261-1272
2] Nomura, N., Yamagishi, H., and Oka, A. (1978) Gene 3, 39-52.2.
3] Oka, A., Nomura, N., Sugimoto, K., Sugisaki, H., and Takanami, M. (1978) Nature 276, 845-847.
4] Grindley, N.D.F., and Joyce, C.M. (1980) Proc. Natl. Acad. Sci., USA 77, 7176- 7180.
5] Oka, A., Sugisaki, H., and Takanami, M. (1981) J. Mol. Biol. 147, 217-226.
6] Weinert, T.A., Schaus, N.A., and Grindley, N.D.F. (1983) Science 222, 755-765.
7] Mollet, B., Clerget, M., Meyer, J., and Iida, S. (1985) J. Bacteriol. 163, 55-60.
8] Mollet, B., Iida, S., and Arber, W. (1985) Mol. Gen. Genet. 199, 534-536.
9] Derbyshire, K.M., Hwang, L., and Grindley, N.D.F. (1987) Proc. Natl. Acad. Sci., USA 84, 8049-8053.
10] Stibitz, S., and Davies, J. (1987) Plasmid 17, 202-209.
11] Derbyshire, K.M., Kramer, M., and Grindley, N.D.F. (1990) Proc. Natl. Acad. Sci., USA 87, 4048-4052.
12] Bernardi, F., and Bernardi, A. (1991) Mol. Gen. Genet. 227, 22-27.
13] Derbyshire, K.M., and Grindley, N.D.F. (1992) EMBO J. 11, 3449-3455.
14] Wiater, L., Sadosky, A.B., and Shuman, H.A. (1994) Mol. Microbiol. 11, 641-653.
15] Derbyshire, K.M., and Grindley, N.D.F. (1996) Mol. Microbiol. 21, 1261-1272