IS881
- Family IS5
- Group IS5
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_005241 | ND | Ralstonia eutropha | Ralstonia eutropha megaplasmid pHG1 |
DNA section
IS Length : 3033 bp
Ends
IR Length : 13/16
IRL : GGCAATGCTGATTAAGTCTTTCGGTTCGTGAGAGTAAGCGTTATCTGAAG
IRR : GGCTATGCTGAACAAGCCGCGTGAATTGGTGTGTTCAGACTGATAAGAAC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GGCAATGCTG | TTAG | TCGCCAGCCG | 4 |
DNA sequence
GGCAATGCTGATTAAGTCTTTCGGTTCGTGAGAGTAAGCGTTATCTGAAGAATCACGAGCGCGAAGAGATGAACATGAAGCAGATGACGTTCGCGGCGGG
TGCAGATCAAGAGGCGGGATTCGAGCAATACCGTAAGCCAACGCGACGCGAGACATTTCTGGCGATGATGGAGACGATCGTGCCGTGGGCGGCATTGTGC
GCCGTGATCGAGCCGTACTATCCGAAGGCGGGCAACGGCCGGCCGCCCATTGGCCTGGAACGGATGCTGCGCATCCACTTTTTGCAGCACTGGTTCAACC
TGGCGGATCTGGCCTGCGAAGAGGCGCTGTATGACAGCGCCAGCCTGCGCCGCTTCGTTGGGATTGACCTGGGTCGTGAACCGGTGCCGGACGCCAGCAC
GCTGCTGAAGTTTCGCCGGTTACTGGAGAAGCACCAGCTTGCCGAGCGGCTGTTCGCTGAGGTTGGCCGGGTCCTCCAAACCAACGGTGTAAAGCTGAAG
ACTGGCACCATTGTGGATGCCACCATCATCGGCGCACCGAGTTCGAGGAAGAATGAAGAGAAGTCGCGCGAGCCGGAGATGCACCAGACTCGCAAAGGGA
AGCAATGGTGTGTGTCACGAACGCAGGCTTAAGTCTGCGATGCTGTACTGAAGATGGGAGAAGGCCTCCCGGAGTCGGCTTGCAGGAGCAGATTCCAAAC
CACCCGGTGCTGCTGCGTTCAAAAGGCGTGGTGGTGAGCGACCGGGGACGTCATCCGCAGAGGATGGCGGGTACGTGCTGAGAAAGCAAACCGTCCGATG
ACGCATCGTTATTGCCAGGGCGCTGTCGAAACTGAGGCCGGCCATTCGCTTCAGGACAAACCGGGACGACACCTGTTTACGGGTCCGGTGGCAGCCGGTG
TATAGGCGGCATGATCTCAGCGCAGGCTTTGGTACGGAACGTGAGAATCTTGCTCCAGATGTGAAGGGAAATGACAAATGGCAGACACCATGAGGAAGAA
TACCGATGCTGGAGTGAGGGGCGGAGCCGCCCGTAGTAGCGTTGAAGTTCCTGTAATGGGAATGGAGCGAAGGGGCGGCGTTATCCTGCTGATGAACGCG
GTCAACCTGCGGGGATGAGCGGCGTGAAATCGGCGAAGCCGTACAACATTGCGAAGCGCACGGTATGGGAGGCATACCAGCAGGTCAAGGCCAACCGTGG
CGCTGCCGGGATCGACGATGAAGCCATCGCCGAGTTCGAGCAGAACCTGTCGAAGAATCTGTACAAGCTGTGGAACCGGATGCCATCGGGGTCGTATCTC
CCGCCACCGGTCAAGCAAGTGGAGATTCCCAAGGCGTCGGGAGGTACGAGAAAGCTGGGGGTGCCCACGGTCGCAGACCGTGTTGCGCAAACCGTGGTCA
AACTGGTCATTGAGCCGGGTCTCGACGCGATCTTCCATCCGGACTCCTACGGGTACCGGCCGGGGCGGTCTGCGAAGCAGGCGGTAGCGATCACTCGTGA
ACGTTGCTGGCGATATGACTGGGTGGTAGAATTTGACATCAAGGCAGCCTTTGATCAGATCGATCATGGACTGCTGATGAAAGCGGTGCGCACCCACATC
AGGGAAGACTGGATACTGCTATGCATCGAACGGTGGCGGGTTGCACCGTTCGAGACAGCGGACGGGGTGCGTGTGCCCCGCACGCGGGGAACACCCCAGG
GCGGTGTGAGCAGTCCGATCCTGATGAACCTGTTCACGCATTACACATTCGACCGATGGATGCAGCGCACCAGCCCAAACTGTCCGTTTGCCCGCTACGC
GGATGATGCGGTGGTTCACTGCAATAGCCGCAGGCAGGCGGAATACGTGATGCGGTCCATTGCAGCACGGCTGGCAGCTTGTGGGCTGACGATGCATCCG
GAGAAATCGAAGATCGTGTACTGCAGAGACAGCGCAACCGCAGCGAACGCCATCTGCACGCGAGCTTTACGTTTCTCGGTTTTACGTTTAGGCCGAGGAA
AGCGCTCAGCCCGCAAGGGGGGCCTTTTACCAGCTTCCTGCCGGGAGCCAGCGAGAGTGCCTTGAAGCGGATGCGGCAGGCAGTGCGGGGTTGGCGACTC
AATCGCCAGACCCATGTAACACTTGCCGATGTGGCGAGACTGTACAACCCAGTGATACAGGGGTGGTGGAATTACTATGGCGCGTTCTACAAGACTGCGA
TGCTTCACATCTTCCAGCACATCGACTGCGCTCTTGAGCGTTGGGCCCGACGGAAGTACAAGGGTCTGCACAGGCGCAAGCGACGAAGTGCCGAATGGCT
GGACAGGATGCGCCGTAACAACCCGATGCTGTTCGCTCACTGGCGTGTGGTTACGCAACCGGCTGGATAACGGGAGCCCTATGACGCGAGAGTGTCACGT
ACGGTTCTGCGAGAGGCTCGGGGGGCGGTTCCCCGGGTCTACTTACCACTTCGGCATGAAGCTCCACATTGGCGTGGACAGTCAGATTGGGTTGGCCCAT
TCGGCAGTCGTAACGCCAGCCAATGTTCATGACAAGCATGCAGTGCACCAGCTATTGCATGGGCAGGAGCAGCGAGTCTATGGCGATAGCGCCTACGCCA
GTCAGCAGGCGCTGATCGCGTCCAAGGCGCCCCGCGCCAAGGACTTCACCAATCAGCGTACGCGCAAGAGCGGGCAGATTGACGAGGTGCAACGTGGGAA
GAACCGCAACAAATCGAAGATCCGTGCTCGGGTCGAGCATGTGTTTGCCGTAGTCAAACGCCTGTGGGGCTTCACTAAAGTACGCTACCGTGGCTTGAAG
AAGAATGCCGGCCGGGCCTTCACGGCCCTGGCGCTGGCCAATCTGTACCTCAGCCGAGGGCATTTGATGGGAGCAGTCCGTCCGTAAAGGGCGAATGTGG
GGCAAGTGCTCCGCCAACACGCCCCATTTGGGATGCGGCAGGAAAAATTCGCGTCATCAGAGCCGAGTTTTTCGACCGCACACGTTCTTATCAGTCTGAA
CACACCAATTCACGCGGCTTGTTCAGCATAGCC
TGCAGATCAAGAGGCGGGATTCGAGCAATACCGTAAGCCAACGCGACGCGAGACATTTCTGGCGATGATGGAGACGATCGTGCCGTGGGCGGCATTGTGC
GCCGTGATCGAGCCGTACTATCCGAAGGCGGGCAACGGCCGGCCGCCCATTGGCCTGGAACGGATGCTGCGCATCCACTTTTTGCAGCACTGGTTCAACC
TGGCGGATCTGGCCTGCGAAGAGGCGCTGTATGACAGCGCCAGCCTGCGCCGCTTCGTTGGGATTGACCTGGGTCGTGAACCGGTGCCGGACGCCAGCAC
GCTGCTGAAGTTTCGCCGGTTACTGGAGAAGCACCAGCTTGCCGAGCGGCTGTTCGCTGAGGTTGGCCGGGTCCTCCAAACCAACGGTGTAAAGCTGAAG
ACTGGCACCATTGTGGATGCCACCATCATCGGCGCACCGAGTTCGAGGAAGAATGAAGAGAAGTCGCGCGAGCCGGAGATGCACCAGACTCGCAAAGGGA
AGCAATGGTGTGTGTCACGAACGCAGGCTTAAGTCTGCGATGCTGTACTGAAGATGGGAGAAGGCCTCCCGGAGTCGGCTTGCAGGAGCAGATTCCAAAC
CACCCGGTGCTGCTGCGTTCAAAAGGCGTGGTGGTGAGCGACCGGGGACGTCATCCGCAGAGGATGGCGGGTACGTGCTGAGAAAGCAAACCGTCCGATG
ACGCATCGTTATTGCCAGGGCGCTGTCGAAACTGAGGCCGGCCATTCGCTTCAGGACAAACCGGGACGACACCTGTTTACGGGTCCGGTGGCAGCCGGTG
TATAGGCGGCATGATCTCAGCGCAGGCTTTGGTACGGAACGTGAGAATCTTGCTCCAGATGTGAAGGGAAATGACAAATGGCAGACACCATGAGGAAGAA
TACCGATGCTGGAGTGAGGGGCGGAGCCGCCCGTAGTAGCGTTGAAGTTCCTGTAATGGGAATGGAGCGAAGGGGCGGCGTTATCCTGCTGATGAACGCG
GTCAACCTGCGGGGATGAGCGGCGTGAAATCGGCGAAGCCGTACAACATTGCGAAGCGCACGGTATGGGAGGCATACCAGCAGGTCAAGGCCAACCGTGG
CGCTGCCGGGATCGACGATGAAGCCATCGCCGAGTTCGAGCAGAACCTGTCGAAGAATCTGTACAAGCTGTGGAACCGGATGCCATCGGGGTCGTATCTC
CCGCCACCGGTCAAGCAAGTGGAGATTCCCAAGGCGTCGGGAGGTACGAGAAAGCTGGGGGTGCCCACGGTCGCAGACCGTGTTGCGCAAACCGTGGTCA
AACTGGTCATTGAGCCGGGTCTCGACGCGATCTTCCATCCGGACTCCTACGGGTACCGGCCGGGGCGGTCTGCGAAGCAGGCGGTAGCGATCACTCGTGA
ACGTTGCTGGCGATATGACTGGGTGGTAGAATTTGACATCAAGGCAGCCTTTGATCAGATCGATCATGGACTGCTGATGAAAGCGGTGCGCACCCACATC
AGGGAAGACTGGATACTGCTATGCATCGAACGGTGGCGGGTTGCACCGTTCGAGACAGCGGACGGGGTGCGTGTGCCCCGCACGCGGGGAACACCCCAGG
GCGGTGTGAGCAGTCCGATCCTGATGAACCTGTTCACGCATTACACATTCGACCGATGGATGCAGCGCACCAGCCCAAACTGTCCGTTTGCCCGCTACGC
GGATGATGCGGTGGTTCACTGCAATAGCCGCAGGCAGGCGGAATACGTGATGCGGTCCATTGCAGCACGGCTGGCAGCTTGTGGGCTGACGATGCATCCG
GAGAAATCGAAGATCGTGTACTGCAGAGACAGCGCAACCGCAGCGAACGCCATCTGCACGCGAGCTTTACGTTTCTCGGTTTTACGTTTAGGCCGAGGAA
AGCGCTCAGCCCGCAAGGGGGGCCTTTTACCAGCTTCCTGCCGGGAGCCAGCGAGAGTGCCTTGAAGCGGATGCGGCAGGCAGTGCGGGGTTGGCGACTC
AATCGCCAGACCCATGTAACACTTGCCGATGTGGCGAGACTGTACAACCCAGTGATACAGGGGTGGTGGAATTACTATGGCGCGTTCTACAAGACTGCGA
TGCTTCACATCTTCCAGCACATCGACTGCGCTCTTGAGCGTTGGGCCCGACGGAAGTACAAGGGTCTGCACAGGCGCAAGCGACGAAGTGCCGAATGGCT
GGACAGGATGCGCCGTAACAACCCGATGCTGTTCGCTCACTGGCGTGTGGTTACGCAACCGGCTGGATAACGGGAGCCCTATGACGCGAGAGTGTCACGT
ACGGTTCTGCGAGAGGCTCGGGGGGCGGTTCCCCGGGTCTACTTACCACTTCGGCATGAAGCTCCACATTGGCGTGGACAGTCAGATTGGGTTGGCCCAT
TCGGCAGTCGTAACGCCAGCCAATGTTCATGACAAGCATGCAGTGCACCAGCTATTGCATGGGCAGGAGCAGCGAGTCTATGGCGATAGCGCCTACGCCA
GTCAGCAGGCGCTGATCGCGTCCAAGGCGCCCCGCGCCAAGGACTTCACCAATCAGCGTACGCGCAAGAGCGGGCAGATTGACGAGGTGCAACGTGGGAA
GAACCGCAACAAATCGAAGATCCGTGCTCGGGTCGAGCATGTGTTTGCCGTAGTCAAACGCCTGTGGGGCTTCACTAAAGTACGCTACCGTGGCTTGAAG
AAGAATGCCGGCCGGGCCTTCACGGCCCTGGCGCTGGCCAATCTGTACCTCAGCCGAGGGCATTTGATGGGAGCAGTCCGTCCGTAAAGGGCGAATGTGG
GGCAAGTGCTCCGCCAACACGCCCCATTTGGGATGCGGCAGGAAAAATTCGCGTCATCAGAGCCGAGTTTTTCGACCGCACACGTTCTTATCAGTCTGAA
CACACCAATTCACGCGGCTTGTTCAGCATAGCC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 2813 bp | 324 aa | 75 | 2887 | + | No |
Chemistry : DDE
ORF sequence :
MKQMTFAAGADQEAGFEQYRKPTRRETFLAMMETIVPWAALCAVIEPYYPKAGNGRPPIGLERMLRIHFLQHWFNLADLACEEALYDSASLRRFVGIDLG
REPVPDASTLLKFRRLLEKHQLAERLFAEVGRVLQTNGVKLKTGTIVDATIIGAPSSRKNEEKSREPEMHQTRKGKQWHFGMKLHIGVDSQIGLAHSAVV
TPANVHDKHAVHQLLHGQEQRVYGDSAYASQQALIASKAPRAKDFTNQRTRKSGQIDEVQRGKNRNKSKIRARVEHVFAVVKRLWGFTKVRYRGLKKNAG
RAFTALALANLYLSRGHLMGAVRP
REPVPDASTLLKFRRLLEKHQLAERLFAEVGRVLQTNGVKLKTGTIVDATIIGAPSSRKNEEKSREPEMHQTRKGKQWHFGMKLHIGVDSQIGLAHSAVV
TPANVHDKHAVHQLLHGQEQRVYGDSAYASQQALIASKAPRAKDFTNQRTRKSGQIDEVQRGKNRNKSKIRARVEHVFAVVKRLWGFTKVRYRGLKKNAG
RAFTALALANLYLSRGHLMGAVRP
Blast result :
Comments
The transposase of IS881 is interrupted by a group II intron (609..2447). The IS881 transposase was constructed by deletion in silico of the intron sequence. The lenght of the IS881 without the intron sequence is 1195 bp (transposase coordinates : 75-1049).
IS881 is 64% aa similar to IS1021.
IS881 is 64% aa similar to IS1021.
References
1] Schwartz,E., Henne,A., Cramm,R., Eitinger,T., Friedrich,B. and Gottschalk,G.(2003) J. Mol. Biol. 332 (2), 369-383