IS801
- Family IS91
- Group
Isoform Synonym(s) RS-I
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| X57269 | Y | Pseudomonas syringae | Pseudomonas syringae pv. phaseolicola LR781 plasmid pMMC7105 |
DNA section
IS Length : 1512 bp
Ends
oriIS : GAACAGGGCAGCCTGCGGCTGCAAATCGCCCGTTCTTACGATCGCGTAGCAGCAGAAACATCGATTTGAGCTTTGCCATGAGGCCCGTTATTGACCATCA II struct. : No
terIS : TAAGTAACGCATCAGCGTCTTCCTACCCATCGCTTTGAAGGCATGACGCCTCCGTCATAGCGTCAGCCTGATGCATAGGCATTTTGAAATTCCTATGCAT II struct. : No
Insertion site
| Left flank | LE cleavage site | Right flank | RE cleavage site |
|---|---|---|---|
| AGATGAAATT | GAACGCGCGA | 0 | |
| TTTTAATTGT | GGACGAATCG | 0 | |
| TCCTATGCAT | GAACGTGTCG | 0 | |
| ACACAACATA | CGAGCCGGAA | 0 | |
| TGCAGGCATG | CAAGCTTGGC | 0 | |
| ATTAGCAGAG | CGAGGTATGT | 0 | |
| TTTTGTTTGG | CAAGCAGCAG | 0 | |
| NNNNNAAATC | GAACTCTNNN | 0 | |
| NNNTTTCTGC | GGACTGGNNN | 0 | |
| NNGCCCACTG | CAAGCTACCN | 0 | |
| TTGACAAAAA | GAACCGGGCG | 0 | |
| NNNTTGGCGG | CAAGAAAGCC | 0 | |
| NTTGCTTCGG | CAAGCTGCTG | 0 |
DNA sequence
GAACAGGGCAGCCTGCGGCTGCAAATCGCCCGTTCTTACGATCGCGTAGCAGCAGAAACATCGATTTGAGCTTTGCCATGAGGCCCGTTATTGACCATCA
TGAAGCCTGCTTACCCACCGCTGCTGCTTCAGATGAGTCCTGCCTACACACCTCGACCGCTCAAAAACCTCTTCACCGCTAACCAGTGCTGGGCACACCT
CCTCGAGGAGGGCGGCTTACGCGACATCGAAGTCGAGACCGTCACCAAAATGCTCGCCTGCGGCACCTCGATACTGGGCGTCAAACACTACACCTGCGGC
AACCACAGCTGCCCGCACGTCAAATACCTGTGCAACACCTGCCATTGCCGGGCCTGTCCCTCCTGCGGCAAAAAGGCCACCGACCAGTGGATCACCGTGC
AAAACAACCGTCTGCCCGACTGCCCCTGGCAGCATCTGGTGTTCACGCTGCCCGACACGCTATGGCCCCTGTTCTTCTACAACCGCTGGCTGCTCGATGC
GCTGTTTCGGCTGGCGGCCGATAACCTGATCTACGCCGCCAAGCGGCGCGGTTTGCGCGTCGGGATTTTCGGGGCGCTGCACACCTATGGCCGGCGGCTC
AACTGGCATCCCCACGTCCACCTGTCGGTGACCGCGGGCGGCCTGGATGAGCAGGGCGTCTGGAAAAATCTGTCGTTCCACAAAGAGGCCCTGCGGCGGC
GCTGGATGTGGCTGGTGCGCGATTACCTGCTGGGACAGCCGCTTTCGCAACTGACGATGCCGCCGCCGCTGGCCCACATCCTCTGTGAAAGCGACTGGCG
CCGTTTGATACTGGCCGCTGGTGGCCAGCACTGGCACATCCACTTGTCGAAGAAGACGAAAAACGGCCGAAAGACCGTCAATTACCTGGGCCGCTACCTG
AAAAAACCGCCCATCTCGGGCAGTCGTCTGGCGCATTACACCAACGGGGCCACGTTGAGGTTCACCTACCTGGATCACCGCACACAGGCCTATCAGCAGG
AAACGCTGAGCCAGGCCGACATGCTTTTCCGGGTGGTGCAGCACATCCCGGAAAAGCATTTCCGGATGATCCGGTATTTTGGTTTTCTGGCCAATCGGGT
CTGCGGGCAGTACCTGCCAAAGGTCTATGAAGCACTGAAGATGGCGACGCCAGGACCGACACCGAAGCTGTATTTTGCGCCGATGGCCAAAGCCTTTCTA
AACGTCGATCCGTTCCGCTGCGTGCTGTGTGGCGCGCGGATGGTATACACGGCGGCAATCAGCGGGCTGACGGTGCAGGGACTGAACCTCAACGCTCAGG
CGATTGCGCAGATGAGGTACGTGAAGCCCTGACAGGGGGTAGGTGCGTCCGCACCTAGGCTTCGCGGTGAAATAACCTGCATTAATGCTTGTTGATAGGG
ATTTTCACTGCATAAGTAACGCATCAGCGTCTTCCTACCCATCGCTTTGAAGGCATGACGCCTCCGTCATAGCGTCAGCCTGATGCATAGGCATTTTGAA
ATTCCTATGCAT
TGAAGCCTGCTTACCCACCGCTGCTGCTTCAGATGAGTCCTGCCTACACACCTCGACCGCTCAAAAACCTCTTCACCGCTAACCAGTGCTGGGCACACCT
CCTCGAGGAGGGCGGCTTACGCGACATCGAAGTCGAGACCGTCACCAAAATGCTCGCCTGCGGCACCTCGATACTGGGCGTCAAACACTACACCTGCGGC
AACCACAGCTGCCCGCACGTCAAATACCTGTGCAACACCTGCCATTGCCGGGCCTGTCCCTCCTGCGGCAAAAAGGCCACCGACCAGTGGATCACCGTGC
AAAACAACCGTCTGCCCGACTGCCCCTGGCAGCATCTGGTGTTCACGCTGCCCGACACGCTATGGCCCCTGTTCTTCTACAACCGCTGGCTGCTCGATGC
GCTGTTTCGGCTGGCGGCCGATAACCTGATCTACGCCGCCAAGCGGCGCGGTTTGCGCGTCGGGATTTTCGGGGCGCTGCACACCTATGGCCGGCGGCTC
AACTGGCATCCCCACGTCCACCTGTCGGTGACCGCGGGCGGCCTGGATGAGCAGGGCGTCTGGAAAAATCTGTCGTTCCACAAAGAGGCCCTGCGGCGGC
GCTGGATGTGGCTGGTGCGCGATTACCTGCTGGGACAGCCGCTTTCGCAACTGACGATGCCGCCGCCGCTGGCCCACATCCTCTGTGAAAGCGACTGGCG
CCGTTTGATACTGGCCGCTGGTGGCCAGCACTGGCACATCCACTTGTCGAAGAAGACGAAAAACGGCCGAAAGACCGTCAATTACCTGGGCCGCTACCTG
AAAAAACCGCCCATCTCGGGCAGTCGTCTGGCGCATTACACCAACGGGGCCACGTTGAGGTTCACCTACCTGGATCACCGCACACAGGCCTATCAGCAGG
AAACGCTGAGCCAGGCCGACATGCTTTTCCGGGTGGTGCAGCACATCCCGGAAAAGCATTTCCGGATGATCCGGTATTTTGGTTTTCTGGCCAATCGGGT
CTGCGGGCAGTACCTGCCAAAGGTCTATGAAGCACTGAAGATGGCGACGCCAGGACCGACACCGAAGCTGTATTTTGCGCCGATGGCCAAAGCCTTTCTA
AACGTCGATCCGTTCCGCTGCGTGCTGTGTGGCGCGCGGATGGTATACACGGCGGCAATCAGCGGGCTGACGGTGCAGGGACTGAACCTCAACGCTCAGG
CGATTGCGCAGATGAGGTACGTGAAGCCCTGACAGGGGGTAGGTGCGTCCGCACCTAGGCTTCGCGGTGAAATAACCTGCATTAATGCTTGTTGATAGGG
ATTTTCACTGCATAAGTAACGCATCAGCGTCTTCCTACCCATCGCTTTGAAGGCATGACGCCTCCGTCATAGCGTCAGCCTGATGCATAGGCATTTTGAA
ATTCCTATGCAT
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1233 bp | 410 aa | 100 | 1332 | + | No |
Chemistry : Y2
ORF sequence :
MKPAYPPLLLQMSPAYTPRPLKNLFTANQCWAHLLEEGGLRDIEVETVTKMLACGTSILGVKHYTCGNHSCPHVKYLCNTCHCRACPSCGKKATDQWITV
QNNRLPDCPWQHLVFTLPDTLWPLFFYNRWLLDALFRLAADNLIYAAKRRGLRVGIFGALHTYGRRLNWHPHVHLSVTAGGLDEQGVWKNLSFHKEALRR
RWMWLVRDYLLGQPLSQLTMPPPLAHILCESDWRRLILAAGGQHWHIHLSKKTKNGRKTVNYLGRYLKKPPISGSRLAHYTNGATLRFTYLDHRTQAYQQ
ETLSQADMLFRVVQHIPEKHFRMIRYFGFLANRVCGQYLPKVYEALKMATPGPTPKLYFAPMAKAFLNVDPFRCVLCGARMVYTAAISGLTVQGLNLNAQ
AIAQMRYVKP
QNNRLPDCPWQHLVFTLPDTLWPLFFYNRWLLDALFRLAADNLIYAAKRRGLRVGIFGALHTYGRRLNWHPHVHLSVTAGGLDEQGVWKNLSFHKEALRR
RWMWLVRDYLLGQPLSQLTMPPPLAHILCESDWRRLILAAGGQHWHIHLSKKTKNGRKTVNYLGRYLKKPPISGSRLAHYTNGATLRFTYLDHRTQAYQQ
ETLSQADMLFRVVQHIPEKHFRMIRYFGFLANRVCGQYLPKVYEALKMATPGPTPKLYFAPMAKAFLNVDPFRCVLCGARMVYTAAISGLTVQGLNLNAQ
AIAQMRYVKP
Blast result :
Comments
By analogy to the ends of IS91 (Mendiola et al., 1992), the originally proposed limits of IS801 (Romantschuk et al., 1991) have been reconsidered: the first bp is now considered not belonging to the IS, whereas one additional bp from the "target" is now included in the IS (all the coordinates have been changed accordingly).
Based on this scenario, the first four bp of IS801 are identical to the first four bp downstream to the insertion site (in the three cases described).
Based on this scenario, the first four bp of IS801 are identical to the first four bp downstream to the insertion site (in the three cases described).
References