IS66
- Family IS66
- Group
Isoform IS66Synonym(s) IS66-1, IS66v1, IS66t, IS66v2, IS66-1, I
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AF242881 | ND | Agrobacterium tumefaciens | Rhizobium fredii USDA205 Agrobacterium tumefaciens plasmid octopine-type Ti Agrobacterium tumefaciens plasmid pTil5955 Agrobacterium tumefaciens A66 plasmid pTiA66 Agrobacterium tumefaciens A6 Agrobacterium tumefaciens A277 plasmid pTiB6806 Agrobacterium tumefaciens B6S3 plasmid pTiB6S3 Rhizobium fredii USDA193 plasmid pSym Rhizobium fredii USDA194 Rhizobium fredii USDA201 |
DNA section
IS Length : 2556 bp
Ends
IR Length : 18/20
IRL : GTAAGCCCACGGTGAAGGCCGTCTTGCGATGACGGCCTGCTGACTGCCAG
IRR : GTAAGCCTCCGGTGAAGGCCACAGGTCAGGCAGCTTGAGCGTTGACGGTC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GCCAAATACT | GGGATGGC | CTGAAGCTCT | 8 |
DNA sequence
GTAAGCCCACGGTGAAGGCCGTCTTGCGATGACGGCCTGCTGACTGCCAGTCCTAGTGGTAACACCAGGAGTAGTCATATGGCGAAGCATCCAATTGAGG
TGATCACGTCTGTGGAGCGCCGTCGGCGTTGGTCACGCGAAGATAAAGAGCGGCTCGTCGCTGCGTGCTTTGAGCCAGACGCGGTCATCTCGGAGATTGC
CCGCGCGGCCGGCATCCATGTCAGCCAGTTGTTCCGTTGGCGCAAAGAGCTTTGTCGGATCGAGGAGCCAAGATCTGACACTGCGGCTTTGGTGCCGGTA
ATCGTGTCCGAGGCCGCTTCGACAGTCTCCCCCGCTCAGCCGGAACCACCCACCAAATTCCATCCTCGTCGGAAGCGCAGTGATGTAACAATCGAACTAG
GTCGGGGCCGACGCGTCCGCGTGGAAAGCGACATCGACACGGACGCGCTTGGCCGGATTCTCGATTGCGTGCTGGGGCTGCGATGATCCCGGTTCCTGTT
GGCGTGAAGGTCTGGCTGGCGACGGGCCATACCGATATGCGCAAAGGCTTCCCCGGTCTGTCGTTGATGGTGCAGGAGGCGCTGAAGCGCGACCCGATGT
GCGGTCACCTGTTCGTATTCCGCGGCCGAGGCGGTGGCCTGATCAAGGTGATCTGGCATGACGGCCAGGGAGCTTGCCTGTTCACGAAGAAGCTTGAGCG
AGGACGCTTCATCTGGCCGTCAGCGGCCGATCGCACGGTGGTGATCACGCCTGCGCAGCTCGGTTATCTGCTGGAGGGCATCGACTGGCGGATGCCGCAA
AAGACCTGGCGGCCGACGTCGGCCGGATGAGCAAAAACACTGGAATGATGGGGTCAAATATGATTCCATCCTGTCATGAGCGACGCGACCGAAGAGCTTC
CGGACGACCTTGCCAGTGCCCTCGCATTGCTGGCCCAGGAACGCGCTCGACGTGTTGCAGCCGAAGCAGAAGCGGCAACCGCCAAGGCAGAAGCCGCCAG
TGCAAAGGCACTCGTATCGCATTCCGAAGCGCTGATCGCTCGGCTGAAGGTGGAGATCGACAAGGTCCGCCGTGAACTCTACGGCAGCCGGTCCGAGCGT
AAGGCGCGGCTCCTGGAGCAGATGGAACTGCAACTCGAGGAATTGGAAGCGGACGCCGGTGAAGATGAACTGGCCGCGGAGATCGCAGCCAAAGCTTCAA
CCGTCAAAGCCTTCGAGCGCAGGCGTCCGTCACGCAAGCCCTTTCCCGAACATCTGCCACGCGAGCGCATCGTCATCGCCGCTCCCGCCAATTGCGCCTG
CTGCGGATCGGCCAAATTGTCGAAGCTTGGCGAGGACATCACCGAGACCCTGGAGGTCATCCCGCGCCAGTGGAAGGTCATCCAGACGGTACGGGAGAAG
TTTACCTGTCGCGAGTGCGAGAAGATCACGCAGCCACCAGCACCCTTCCATGTAACGCCACGCGGCTTTGCCGGGCCAAACCTGCTGGCGATGATCCTGT
TCGAGAAGTTCGCCCAGCACCAGCCGCTCAATCGTCAGAGCGAACGCTATGCTCGCGAGGGCGTCGACCTTAGTTTATCGACGCTTGCAGATCAGGTCGG
AGCTTGCGCCGCGACGTTGAAGCCACTTCATTCTCTGATCGAAGCGCATGTCCTTTCTGCCGAACGTCTGCACGGCGACGACACGACCGTGCCAATCCTG
GCCAAGGGAAAGACAGATACGGGCCGCATTTGGACCTATGTCCGGGATGATCGGCCGTTCGGAGGCCTCTCACCGCCGGCAGCCCTTTACTATGCCTCGC
GCGATCGACGGCAGGAGCATCCGGAACGCCACCTGAAGACCTTCACCGGTATTCTGCAGGCGGACGCCTATGGCGGCTACAATCCGCTGTTCAAGGGTGA
TCGCGATCCAAATCCGCTAAGACAGGCGTTTTGTTGGGCACACGCGCGTCGCAAGTTCTTCGTGCTCGCAGACATTAATGCGAACGCCAAGCGCGGAAAG
AACGCCGCGCCGATCTCGCCTATGGCGCTCGAAGCTGTCAAACGGATCGACGGCCTGTTCGATATCGAGCGGGAGATCAACGGGCTTACGGCCGATCAAC
GCCTGGAACGTCGCCGCAAGGAAAGCCTGCCGCTCGTCGACAGTCTGCAGGTCTGGCTTCAAACCGAGCGTGCAAAACTGTCGCGCAGTTCTCCGGTCGC
CGAGGCGATCGATTACATGCTCAAGCGTTGGGATGGCTTCACATCATTCCTGGAGGATGGCCGGATTTGCCTCACGAACAACGCCGCGGAGCGAGCCCTG
CGAGGCTTCGCACTCGGTAGGAAGTCCTGGCTCTTCGCCGGATCAGACCGCGGCGCGGATCGTGCAGCCTTCATGGCCACGTTGATCATGACGGCAAAGC
TCAACGACATCGATCCGCAGGCGTGGCTGGCCGACGTTCTTGCCCGCATCGCCGACACGCCGATTATCAGGCTGGAGCAGTTGCTTCCGTGGAATTGGAC
GCCGCCGACCGTCAACGCTCAAGCTGCCTGACCTGTGGCCTTCACCGGAGGCTTAC
TGATCACGTCTGTGGAGCGCCGTCGGCGTTGGTCACGCGAAGATAAAGAGCGGCTCGTCGCTGCGTGCTTTGAGCCAGACGCGGTCATCTCGGAGATTGC
CCGCGCGGCCGGCATCCATGTCAGCCAGTTGTTCCGTTGGCGCAAAGAGCTTTGTCGGATCGAGGAGCCAAGATCTGACACTGCGGCTTTGGTGCCGGTA
ATCGTGTCCGAGGCCGCTTCGACAGTCTCCCCCGCTCAGCCGGAACCACCCACCAAATTCCATCCTCGTCGGAAGCGCAGTGATGTAACAATCGAACTAG
GTCGGGGCCGACGCGTCCGCGTGGAAAGCGACATCGACACGGACGCGCTTGGCCGGATTCTCGATTGCGTGCTGGGGCTGCGATGATCCCGGTTCCTGTT
GGCGTGAAGGTCTGGCTGGCGACGGGCCATACCGATATGCGCAAAGGCTTCCCCGGTCTGTCGTTGATGGTGCAGGAGGCGCTGAAGCGCGACCCGATGT
GCGGTCACCTGTTCGTATTCCGCGGCCGAGGCGGTGGCCTGATCAAGGTGATCTGGCATGACGGCCAGGGAGCTTGCCTGTTCACGAAGAAGCTTGAGCG
AGGACGCTTCATCTGGCCGTCAGCGGCCGATCGCACGGTGGTGATCACGCCTGCGCAGCTCGGTTATCTGCTGGAGGGCATCGACTGGCGGATGCCGCAA
AAGACCTGGCGGCCGACGTCGGCCGGATGAGCAAAAACACTGGAATGATGGGGTCAAATATGATTCCATCCTGTCATGAGCGACGCGACCGAAGAGCTTC
CGGACGACCTTGCCAGTGCCCTCGCATTGCTGGCCCAGGAACGCGCTCGACGTGTTGCAGCCGAAGCAGAAGCGGCAACCGCCAAGGCAGAAGCCGCCAG
TGCAAAGGCACTCGTATCGCATTCCGAAGCGCTGATCGCTCGGCTGAAGGTGGAGATCGACAAGGTCCGCCGTGAACTCTACGGCAGCCGGTCCGAGCGT
AAGGCGCGGCTCCTGGAGCAGATGGAACTGCAACTCGAGGAATTGGAAGCGGACGCCGGTGAAGATGAACTGGCCGCGGAGATCGCAGCCAAAGCTTCAA
CCGTCAAAGCCTTCGAGCGCAGGCGTCCGTCACGCAAGCCCTTTCCCGAACATCTGCCACGCGAGCGCATCGTCATCGCCGCTCCCGCCAATTGCGCCTG
CTGCGGATCGGCCAAATTGTCGAAGCTTGGCGAGGACATCACCGAGACCCTGGAGGTCATCCCGCGCCAGTGGAAGGTCATCCAGACGGTACGGGAGAAG
TTTACCTGTCGCGAGTGCGAGAAGATCACGCAGCCACCAGCACCCTTCCATGTAACGCCACGCGGCTTTGCCGGGCCAAACCTGCTGGCGATGATCCTGT
TCGAGAAGTTCGCCCAGCACCAGCCGCTCAATCGTCAGAGCGAACGCTATGCTCGCGAGGGCGTCGACCTTAGTTTATCGACGCTTGCAGATCAGGTCGG
AGCTTGCGCCGCGACGTTGAAGCCACTTCATTCTCTGATCGAAGCGCATGTCCTTTCTGCCGAACGTCTGCACGGCGACGACACGACCGTGCCAATCCTG
GCCAAGGGAAAGACAGATACGGGCCGCATTTGGACCTATGTCCGGGATGATCGGCCGTTCGGAGGCCTCTCACCGCCGGCAGCCCTTTACTATGCCTCGC
GCGATCGACGGCAGGAGCATCCGGAACGCCACCTGAAGACCTTCACCGGTATTCTGCAGGCGGACGCCTATGGCGGCTACAATCCGCTGTTCAAGGGTGA
TCGCGATCCAAATCCGCTAAGACAGGCGTTTTGTTGGGCACACGCGCGTCGCAAGTTCTTCGTGCTCGCAGACATTAATGCGAACGCCAAGCGCGGAAAG
AACGCCGCGCCGATCTCGCCTATGGCGCTCGAAGCTGTCAAACGGATCGACGGCCTGTTCGATATCGAGCGGGAGATCAACGGGCTTACGGCCGATCAAC
GCCTGGAACGTCGCCGCAAGGAAAGCCTGCCGCTCGTCGACAGTCTGCAGGTCTGGCTTCAAACCGAGCGTGCAAAACTGTCGCGCAGTTCTCCGGTCGC
CGAGGCGATCGATTACATGCTCAAGCGTTGGGATGGCTTCACATCATTCCTGGAGGATGGCCGGATTTGCCTCACGAACAACGCCGCGGAGCGAGCCCTG
CGAGGCTTCGCACTCGGTAGGAAGTCCTGGCTCTTCGCCGGATCAGACCGCGGCGCGGATCGTGCAGCCTTCATGGCCACGTTGATCATGACGGCAAAGC
TCAACGACATCGATCCGCAGGCGTGGCTGGCCGACGTTCTTGCCCGCATCGCCGACACGCCGATTATCAGGCTGGAGCAGTTGCTTCCGTGGAATTGGAC
GCCGCCGACCGTCAACGCTCAAGCTGCCTGACCTGTGGCCTTCACCGGAGGCTTAC
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 408 bp | 135 aa | 79 | 486 | + | No |
AG : IS66 TnpA
ORF sequence :
MAKHPIEVITSVERRRRWSREDKERLVAACFEPDAVISEIARAAGIHVSQLFRWRKELCRIEEPRSDTAALVPVIVSEAASTVSPAQPEPPTKFHPRRKR
SDVTIELGRGRRVRVESDIDTDALGRILDCVLGLR
SDVTIELGRGRRVRVESDIDTDALGRILDCVLGLR
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 348 bp | 115 aa | 483 | 830 | + | No |
AG : IS66 TnpB
ORF sequence :
MIPVPVGVKVWLATGHTDMRKGFPGLSLMVQEALKRDPMCGHLFVFRGRGGGLIKVIWHDGQGACLFTKKLERGRFIWPSAADRTVVITPAQLGYLLEGI
DWRMPQKTWRPTSAG
DWRMPQKTWRPTSAG
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1656 bp | 551 aa | 876 | 2531 | + | No |
Chemistry : DDE
ORF sequence :
MSDATEELPDDLASALALLAQERARRVAAEAEAATAKAEAASAKALVSHSEALIARLKVEIDKVRRELYGSRSERKARLLEQMELQLEELEADAGEDELA
AEIAAKASTVKAFERRRPSRKPFPEHLPRERIVIAAPANCACCGSAKLSKLGEDITETLEVIPRQWKVIQTVREKFTCRECEKITQPPAPFHVTPRGFAG
PNLLAMILFEKFAQHQPLNRQSERYAREGVDLSLSTLADQVGACAATLKPLHSLIEAHVLSAERLHGDDTTVPILAKGKTDTGRIWTYVRDDRPFGGLSP
PAALYYASRDRRQEHPERHLKTFTGILQADAYGGYNPLFKGDRDPNPLRQAFCWAHARRKFFVLADINANAKRGKNAAPISPMALEAVKRIDGLFDIERE
INGLTADQRLERRRKESLPLVDSLQVWLQTERAKLSRSSPVAEAIDYMLKRWDGFTSFLEDGRICLTNNAAERALRGFALGRKSWLFAGSDRGADRAAFM
ATLIMTAKLNDIDPQAWLADVLARIADTPIIRLEQLLPWNWTPPTVNAQAA
AEIAAKASTVKAFERRRPSRKPFPEHLPRERIVIAAPANCACCGSAKLSKLGEDITETLEVIPRQWKVIQTVREKFTCRECEKITQPPAPFHVTPRGFAG
PNLLAMILFEKFAQHQPLNRQSERYAREGVDLSLSTLADQVGACAATLKPLHSLIEAHVLSAERLHGDDTTVPILAKGKTDTGRIWTYVRDDRPFGGLSP
PAALYYASRDRRQEHPERHLKTFTGILQADAYGGYNPLFKGDRDPNPLRQAFCWAHARRKFFVLADINANAKRGKNAAPISPMALEAVKRIDGLFDIERE
INGLTADQRLERRRKESLPLVDSLQVWLQTERAKLSRSSPVAEAIDYMLKRWDGFTSFLEDGRICLTNNAAERALRGFALGRKSWLFAGSDRGADRAAFM
ATLIMTAKLNDIDPQAWLADVLARIADTPIIRLEQLLPWNWTPPTVNAQAA
Blast result :
Comments
IS66 is inserted within IS71, in the virG-C intergenic region of the octopine pTi15955 plasmid.
IS66v1 described by Chen et al. (1991) corresponds in fact to the insertion of of IS66 into IS71 (Hooykaas, 1994).
IS66 orfA and orfB is 98% aa similar to IS66-1, and orfC is 81% aa similar to ISAli10.
IS66v1 described by Chen et al. (1991) corresponds in fact to the insertion of of IS66 into IS71 (Hooykaas, 1994).
IS66 orfA and orfB is 98% aa similar to IS66-1, and orfC is 81% aa similar to ISAli10.
References
1] Waldron, C., and Hepburn, A.G. (1983) Plasmid 10, 199-203.
2] Machida, Y., Sakurai, M., Kiyokawa, S., Ubasawa, A., Suzuki, Y., and Ikeda, J.-E (1984) Proc. Natl. Acad. Sci., USA 81, 7495-7499.
3]Thompson,D.V., Melchers,L.S., Idler,K.B., Schilperoort,R.A. and Hooykaas,P.J. (1988) Nucleic Acids Res. 16 (10), 4621-4636.
4] Chen, C.-Y., Wang, L., and Winans, S.C. (1991) Mol. Gen. Genet. 230, 302-309.
5] Hooykaas, P.J.J. (1994) Personal communication.
6] Beijersbergen, A., Idler, K.B., Thompson, D.V., Melchers, L.S., and Hooykaas, P.J.J. (1994) in pp. 37-54. Submitted.
7]Zhu,J., Oger,P.M., Schrammeijer,B., Hooykaas,P.J., Farrand,S.K. and Winans,S.C. (2000) Direct submission GenBank.
8]Schrammeijer,B., Beijersbergen,A., Idler,K.B., Melchers,L.S., Thompson,D.V. and Hooykaas,P.J. (2000) J. Exp. Bot. 51 (347), 1167-1169.
9] Binns, A.N., Sciaky, D., and Wood, H.N. (1982) Cell 31, 605-612.
10] Barker, R.F., Idler, K.B., Thompson, D.V., and Kemp, J.D. (1983) Plant Mol. Biol. 2, 335-350.
13] Sciaky, D., and Thomashow, M.F. (1984) Nucl. Acids Res. 12, 1447-1461.
14] Ramakrishnan, N., Prakash, R.K., and Atherly, A.G. (1986) Plant Mol. Biol. 7, 177- 188.
15] Beijersbergen, A., Idler, K.B., Thompson, D.V., Melchers, L.S., and Hooykaas, P.J.J. (1994) in pp. 37-54. Submitted.
16] Ferat, J.-L., Le Gouar, M., and Michel, F. (1994) C. R. Acad. Sci. Paris 317, 141-148.
17] Knoop, V., and Brennicke, A. (1994) Nucl. Acids Res. 22, 1167-1171.
18] Yeoman, K.H., Hong, G.F., Mimmack, M.L., and Johnston, A.W.B. (1996) Plasmid 35, 121-130
2] Machida, Y., Sakurai, M., Kiyokawa, S., Ubasawa, A., Suzuki, Y., and Ikeda, J.-E (1984) Proc. Natl. Acad. Sci., USA 81, 7495-7499.
3]Thompson,D.V., Melchers,L.S., Idler,K.B., Schilperoort,R.A. and Hooykaas,P.J. (1988) Nucleic Acids Res. 16 (10), 4621-4636.
4] Chen, C.-Y., Wang, L., and Winans, S.C. (1991) Mol. Gen. Genet. 230, 302-309.
5] Hooykaas, P.J.J. (1994) Personal communication.
6] Beijersbergen, A., Idler, K.B., Thompson, D.V., Melchers, L.S., and Hooykaas, P.J.J. (1994) in pp. 37-54. Submitted.
7]Zhu,J., Oger,P.M., Schrammeijer,B., Hooykaas,P.J., Farrand,S.K. and Winans,S.C. (2000) Direct submission GenBank.
8]Schrammeijer,B., Beijersbergen,A., Idler,K.B., Melchers,L.S., Thompson,D.V. and Hooykaas,P.J. (2000) J. Exp. Bot. 51 (347), 1167-1169.
9] Binns, A.N., Sciaky, D., and Wood, H.N. (1982) Cell 31, 605-612.
10] Barker, R.F., Idler, K.B., Thompson, D.V., and Kemp, J.D. (1983) Plant Mol. Biol. 2, 335-350.
13] Sciaky, D., and Thomashow, M.F. (1984) Nucl. Acids Res. 12, 1447-1461.
14] Ramakrishnan, N., Prakash, R.K., and Atherly, A.G. (1986) Plant Mol. Biol. 7, 177- 188.
15] Beijersbergen, A., Idler, K.B., Thompson, D.V., Melchers, L.S., and Hooykaas, P.J.J. (1994) in pp. 37-54. Submitted.
16] Ferat, J.-L., Le Gouar, M., and Michel, F. (1994) C. R. Acad. Sci. Paris 317, 141-148.
17] Knoop, V., and Brennicke, A. (1994) Nucl. Acids Res. 22, 1167-1171.
18] Yeoman, K.H., Hong, G.F., Mimmack, M.L., and Johnston, A.W.B. (1996) Plasmid 35, 121-130