IS605
- Family IS200/IS605
- Group IS605
Isoform Synonym(s) IShp, RS2
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| HPU60177 | ND | Helicobacter pylori | Helicobacter pylori NCTC11638 |
DNA section
IS Length : 1887 bp
Ends
Left end : CCACCCAAGCAGAAATCCCAAATATCTTTAGTGTTTGGGATGAATGCTTTAGTTCCGCTAGGAACTATATAAAAACCTTATGAGTGAAACTAACCTTGAT II struct. : Yes
Right end : AATGGGGTTATTTCTAAATATGGCAACATATTGGATCTAGCTAGTTATGGAGCGATGAAGCAAGAAAAAGCCCAATCGCTTTAGCGGTTGGGTAATTCAC II struct. : Yes
Insertion site
| Left flank | LE cleavage site | Right flank | RE cleavage site |
|---|---|---|---|
| ATCGACACGA | TAAA | TATATTCAAT | tcac |
DNA sequence
CCACCCAAGCAGAAATCCCAAATATCTTTAGTGTTTGGGATGAATGCTTTAGTTCCGCTAGGAACTATATAAAAACCTTATGAGTGAAACTAACCTTGAT
TTTCTATATACGCCTTGATCGTTTCAGGATTAGCTTGACCGATAGAGCAAACAAAAAATCCATCAGTCCAAAAAGTTTTTTCTTTCCAAAAGTGTTTTTG
CAATAAGGGGATAAATCTTTTATCGTGCCAAACTCTATAAGTGGTGATTTGTTTGATTTTAGAAATAATGGAACTAATAGACATTCTAGGGATATATTGA
ACCATTAAGTGCAAATGATCTATATCGCTTTCCATCGCAATGATAATGAAATGACTTTGGGTGGCTATCTCATCTATAACAGACTTAATAAAGTTATTCA
AATCCCCTTGCAACAACTTTTTTCTATACTTACACACTAAAATCAAATGGGCTTTTAGATTATGCTTGCTTCGGTTGGTTGAAATATACCCCCTTAATGG
GTAATGGTTTTTTCTCATTCCTCTATCCTTATTCATTATTTATAAAAACATTGTATAATAATACAAGATAAAGATAAGGAGTTATTTTTGCTTAACGCTA
TCAAGTTTAGAATTTATCCTAACGCTCAACAAAAAGAGCTTATTTCTAAACATTTTGGCTGTTCTAGGGTCGTGTATAACTACTTTTTAGATTACCGACA
AAAGCAATACGCAAAAGGCATTAAAGAAACTTACTTCACCATGCAAAAAGTCTTAACCCAAATCAAGCACCAAGAAAAATACCATTACCTCAATGAATGC
AATTCTCAAAGCTTGCAAATGGCGTTAAGACAGCTTGTGAGTGCTTATGATAATTTCTTTAGCAAAAGAGCGAGATACCCTAAATTCAAATCTAAAAAAA
ACGCTAAACAATCTTTTGCAATCCCCCAAAACATAGAAATCAAAACAGAGACTCAAACCATCGCTCTCCCTAAATTCAAAGAGGGCATTAAGGCTAAATT
ACACAGAGAATTGCCTAAAGATAGCGTTATCAAACAGGCTTTTATTTCTTGCATAGCCGATCAATATTTTTGTTCTATATCCTATGAAACCAAAGAGCCT
ATCCCTAAACCTACCATCATTAAAAAAGCGGTAGGTTTAGACATGGGCTTAAGAACGCTCATTGTTACAAGCGATAAAATAGAATACCCACACATCCGTT
TTTATCAAAAATTAGAAAAGAAACTCACTAAAGCGCAAAGGAGGTTAAGTAAAAAAGTAAAAGGCTCCAACAACAGGAAAAAACAAGCTAAAAAGGTAGC
TAGATTGCATCTAGCTTGTTCAAACACTAGAGATGACTACTTGCATAAAATCAGTAATGAGATAACCAATCAATACGATTTGATAGGGGTAGAAACTTTG
AATGTTAAGGGGCTTATGAGAACCTATCATTCTAAAAGCCTTGCTAATGCGAGTTGGGGGAAATTCCTTACTATGCTAAAATACAAAGCCCAAAGAAAAG
CTAAAACCCTATTAGGCATAGACAGATTTTTCCCTAGCTCTCAATTGTGTTCTTATTGTGGGTTCAATACAGGCAAAAAACATGAAAACATCACTAAATT
CACTTGTCCTCATTGCAATATCACACACCACAGAGATTACAATGCGAGCGTCAATATTAGAAACTACGCTTTAGGCATGCTAGATGACAGGCATAAAATA
AAGATAGATAAAAGTAGGGTAGGGATTATCCGAACTGATTACGCTCATTACACTGATGAGCGCATCAAAGCTTGTGGAGCTTCCTCTAATGGGGTTATTT
CTAAATATGGCAACATATTGGATCTAGCTAGTTATGGAGCGATGAAGCAAGAAAAAGCCCAATCGCTTTAGCGGTTGGGTAATTCAC
TTTCTATATACGCCTTGATCGTTTCAGGATTAGCTTGACCGATAGAGCAAACAAAAAATCCATCAGTCCAAAAAGTTTTTTCTTTCCAAAAGTGTTTTTG
CAATAAGGGGATAAATCTTTTATCGTGCCAAACTCTATAAGTGGTGATTTGTTTGATTTTAGAAATAATGGAACTAATAGACATTCTAGGGATATATTGA
ACCATTAAGTGCAAATGATCTATATCGCTTTCCATCGCAATGATAATGAAATGACTTTGGGTGGCTATCTCATCTATAACAGACTTAATAAAGTTATTCA
AATCCCCTTGCAACAACTTTTTTCTATACTTACACACTAAAATCAAATGGGCTTTTAGATTATGCTTGCTTCGGTTGGTTGAAATATACCCCCTTAATGG
GTAATGGTTTTTTCTCATTCCTCTATCCTTATTCATTATTTATAAAAACATTGTATAATAATACAAGATAAAGATAAGGAGTTATTTTTGCTTAACGCTA
TCAAGTTTAGAATTTATCCTAACGCTCAACAAAAAGAGCTTATTTCTAAACATTTTGGCTGTTCTAGGGTCGTGTATAACTACTTTTTAGATTACCGACA
AAAGCAATACGCAAAAGGCATTAAAGAAACTTACTTCACCATGCAAAAAGTCTTAACCCAAATCAAGCACCAAGAAAAATACCATTACCTCAATGAATGC
AATTCTCAAAGCTTGCAAATGGCGTTAAGACAGCTTGTGAGTGCTTATGATAATTTCTTTAGCAAAAGAGCGAGATACCCTAAATTCAAATCTAAAAAAA
ACGCTAAACAATCTTTTGCAATCCCCCAAAACATAGAAATCAAAACAGAGACTCAAACCATCGCTCTCCCTAAATTCAAAGAGGGCATTAAGGCTAAATT
ACACAGAGAATTGCCTAAAGATAGCGTTATCAAACAGGCTTTTATTTCTTGCATAGCCGATCAATATTTTTGTTCTATATCCTATGAAACCAAAGAGCCT
ATCCCTAAACCTACCATCATTAAAAAAGCGGTAGGTTTAGACATGGGCTTAAGAACGCTCATTGTTACAAGCGATAAAATAGAATACCCACACATCCGTT
TTTATCAAAAATTAGAAAAGAAACTCACTAAAGCGCAAAGGAGGTTAAGTAAAAAAGTAAAAGGCTCCAACAACAGGAAAAAACAAGCTAAAAAGGTAGC
TAGATTGCATCTAGCTTGTTCAAACACTAGAGATGACTACTTGCATAAAATCAGTAATGAGATAACCAATCAATACGATTTGATAGGGGTAGAAACTTTG
AATGTTAAGGGGCTTATGAGAACCTATCATTCTAAAAGCCTTGCTAATGCGAGTTGGGGGAAATTCCTTACTATGCTAAAATACAAAGCCCAAAGAAAAG
CTAAAACCCTATTAGGCATAGACAGATTTTTCCCTAGCTCTCAATTGTGTTCTTATTGTGGGTTCAATACAGGCAAAAAACATGAAAACATCACTAAATT
CACTTGTCCTCATTGCAATATCACACACCACAGAGATTACAATGCGAGCGTCAATATTAGAAACTACGCTTTAGGCATGCTAGATGACAGGCATAAAATA
AAGATAGATAAAAGTAGGGTAGGGATTATCCGAACTGATTACGCTCATTACACTGATGAGCGCATCAAAGCTTGTGGAGCTTCCTCTAATGGGGTTATTT
CTAAATATGGCAACATATTGGATCTAGCTAGTTATGGAGCGATGAAGCAAGAAAAAGCCCAATCGCTTTAGCGGTTGGGTAATTCAC
Protein section
ORF number : 2
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 447 bp | 148 aa | 536 | 90 | - | No |
Chemistry : Y1
ORF sequence :
MNKDRGMRKNHYPLRGYISTNRSKHNLKAHLILVCKYRKKLLQGDLNNFIKSVIDEIATQSHFIIIAMESDIDHLHLMVQYIPRMSISSIISKIKQITTY
RVWHDKRFIPLLQKHFWKEKTFWTDGFFVCSIGQANPETIKAYIENQG
RVWHDKRFIPLLQKHFWKEKTFWTDGFFVCSIGQANPETIKAYIENQG
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1284 bp | 427 aa | 588 | 1871 | + | No |
AG : TnpB
ORF sequence :
MLNAIKFRIYPNAQQKELISKHFGCSRVVYNYFLDYRQKQYAKGIKETYFTMQKVLTQIKHQEKYHYLNECNSQSLQMALRQLVSAYDNFFSKRARYPKF
KSKKNAKQSFAIPQNIEIKTETQTIALPKFKEGIKAKLHRELPKDSVIKQAFISCIADQYFCSISYETKEPIPKPTIIKKAVGLDMGLRTLIVTSDKIEY
PHIRFYQKLEKKLTKAQRRLSKKVKGSNNRKKQAKKVARLHLACSNTRDDYLHKISNEITNQYDLIGVETLNVKGLMRTYHSKSLANASWGKFLTMLKYK
AQRKAKTLLGIDRFFPSSQLCSYCGFNTGKKHENITKFTCPHCNITHHRDYNASVNIRNYALGMLDDRHKIKIDKSRVGIIRTDYAHYTDERIKACGASS
NGVISKYGNILDLASYGAMKQEKAQSL
KSKKNAKQSFAIPQNIEIKTETQTIALPKFKEGIKAKLHRELPKDSVIKQAFISCIADQYFCSISYETKEPIPKPTIIKKAVGLDMGLRTLIVTSDKIEY
PHIRFYQKLEKKLTKAQRRLSKKVKGSNNRKKQAKKVARLHLACSNTRDDYLHKISNEITNQYDLIGVETLNVKGLMRTYHSKSLANASWGKFLTMLKYK
AQRKAKTLLGIDRFFPSSQLCSYCGFNTGKKHENITKFTCPHCNITHHRDYNASVNIRNYALGMLDDRHKIKIDKSRVGIIRTDYAHYTDERIKACGASS
NGVISKYGNILDLASYGAMKQEKAQSL
Blast result :
Comments
IS605, originally described as RS2 (Bukanov and Berg, 1994), is associated with the virulence-associated region cag. IS605 ORF1 is similar to that of IS200 whereas ORF2 is related to IS1136. There are 9 or ten copies of IS605 in Helicobacter pylori NCTC11638, and about 25 % of the 70 independent clinical isolates of Helicobacter pylori harbour IS605-related elements (Akopyants et al., 1995; Kersulyte et al., 1996). IS605 harbours two divergent ORFs: ORF1, 148 aa (106-552) CS is related to those of IS200 (28 % identity) and ISH1-8 (24 % identity); ORF2, 399 aa (688-1887) is related to those of the IS891 family members (37 % identity with IS1341).
References
1] Bukanov, N.O., and Berg, D.E. (1994) Mol. Microbiol. 11, 509-523.
2] Akopyants, N.S., Kersulyte, D., Clifton, S., Roe, B., and Berg, D.E. (1995) Personal communication.
3] Kersulyte, D., Akopyants, N.S., Clifton, S., Roe, B.S., and Berg, D.E. (1996) IS605, a novel chimaeric transposable element from Helicobacter pylori. Poster at the ASM Meeting, May 1996.
4] Kersulyte, D., Akopyants, N.S., Clifton, S., Roe, B.S., and Berg, D.E. (1998) Gene 223, 175-186.
2] Akopyants, N.S., Kersulyte, D., Clifton, S., Roe, B., and Berg, D.E. (1995) Personal communication.
3] Kersulyte, D., Akopyants, N.S., Clifton, S., Roe, B.S., and Berg, D.E. (1996) IS605, a novel chimaeric transposable element from Helicobacter pylori. Poster at the ASM Meeting, May 1996.
4] Kersulyte, D., Akopyants, N.S., Clifton, S., Roe, B.S., and Berg, D.E. (1998) Gene 223, 175-186.