IS5075
- Family IS110
- Group IS1111
Isoform IS4321LSynonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AF457211 | ND | Escherichia coli | Escherichia coli Klebsiella pneumoniae Klebsiella pneumoniae plasmid pRMH760 Salmonella enetrica serovar Typhi plasmid pHCM1 Acinetobacter baumanii |
DNA section
IS Length : 1327 bp
Ends
IR Length : 12
IRL : taatgagATGGTCACTCCCTCCTTCCCAGTACTATGCTGAGGACAGGCTT
IRR : ----tctATGGTCACTCCCGTTTTTGCAACACCGATTTTGACGATAAATT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GTGCTTTATT | TTCCGTTTTC | 0 | |
| TGCTTTATTT | TCCGTTTTCT | 0 |
DNA sequence
TAATGAGATGGTCACTCCCTCCTTCCCAGTACTATGCTGAGGACAGGCTTTCATTCGGAGAACCATCATGGAAAACATTGCGCTTATTGGTATCGATCTG
GGTAAGAACTCTTTCCATATTCATTGTCAGGATCATCGTGGGAAGGCCGTTTACCGTAAAAAATTCACCCGACCAAAGCTAATCGAATTTCTGGCGACAT
GCCCGGCAACAACCATCGCGATGGAAGCCTGTGGCGGTTCTCACTTTATGGCACGCAAGCTGGCAGAGTTAGGGCATTTTCCAAAGCTGATATCACCGCA
ATTTGTCCGCCCATTCGTTAAAAGCAACAAAAATGACTTCGTTGATGCTGAAGCTATCTGTGAAGCAGCATCACGTCCATCTATGCGTTTCGTGCAGCCC
AGAACCGAATCTCAGCAGGCAATGCGAGCTCTGCATCGTGTCCGTGAATCCCTGGTTCAGGATAAGGTGAAAACAACTAATCAGATGCATGCTTTTCTGC
TGGAATTTGGTATCAGCGTTCCGCGAGGTGCTGCCGTTATTAGTCGACTGAGTACCGTTCTTGAGGACAGTACTTTGCCTCTTTATCTCAGCCAGTTACT
GCTGAAATTACAACAGCATTATCACTATCTTGTTGAGCAGATTAAAGATCTGGAATCTCAGTTGAAACGAAAGTTGGACGAAGATGAGGTTGGACAGCGC
TTGCTGAGTATTCCCTGCGTTGGAACGCTGACTGCCAGTACTATTTCAACTGAGATTGGCGACGGGAAGCAGTACGCCAGCAGCCGTGACTTTGCGGCGG
CAACAGGGCTGGTACCCCGACAGTACAGCACGGGAGGTCGGACGACATTGTTAGGGATTAGCAAGCGGGGCAACAAAAAGATCCGAACTTTGTTGGTTCA
GTGTGCCAGGGTATTCATACAAAAACTGGAACACCAGTCTGGCAAGTTGGCCGACTGGGTCAGGGAGTTGTTGTGTCGGAAAAGCAACTTTGTCGTCACC
TGTGCTCTGGCAAACAAGCTGGCCAGAATAGCCTGGGCACTGACGGCGCGACAGCAAACTTACGAAGCATAAAGGCAGAAATACACCAGTTTAAACAATC
ATTCATCTGGTTTTGCGAATACTGATATTGATGATACTAACGGCCCACCGGCCTGTTGAGGAACCTGTAAAACGGAAAGGCTCATTGAAGCCGTATATTT
TCTGGAGGTTCATCAGGCGCGGAACTCATCGAGGCGCGGGAATAAAATCCCATTCAGACGCCGGATAGATTCAAGCAAGCCAATTTATCGTCAAAATCGG
TGTTGCAAAAACGGGAGTGACCATAGA
GGTAAGAACTCTTTCCATATTCATTGTCAGGATCATCGTGGGAAGGCCGTTTACCGTAAAAAATTCACCCGACCAAAGCTAATCGAATTTCTGGCGACAT
GCCCGGCAACAACCATCGCGATGGAAGCCTGTGGCGGTTCTCACTTTATGGCACGCAAGCTGGCAGAGTTAGGGCATTTTCCAAAGCTGATATCACCGCA
ATTTGTCCGCCCATTCGTTAAAAGCAACAAAAATGACTTCGTTGATGCTGAAGCTATCTGTGAAGCAGCATCACGTCCATCTATGCGTTTCGTGCAGCCC
AGAACCGAATCTCAGCAGGCAATGCGAGCTCTGCATCGTGTCCGTGAATCCCTGGTTCAGGATAAGGTGAAAACAACTAATCAGATGCATGCTTTTCTGC
TGGAATTTGGTATCAGCGTTCCGCGAGGTGCTGCCGTTATTAGTCGACTGAGTACCGTTCTTGAGGACAGTACTTTGCCTCTTTATCTCAGCCAGTTACT
GCTGAAATTACAACAGCATTATCACTATCTTGTTGAGCAGATTAAAGATCTGGAATCTCAGTTGAAACGAAAGTTGGACGAAGATGAGGTTGGACAGCGC
TTGCTGAGTATTCCCTGCGTTGGAACGCTGACTGCCAGTACTATTTCAACTGAGATTGGCGACGGGAAGCAGTACGCCAGCAGCCGTGACTTTGCGGCGG
CAACAGGGCTGGTACCCCGACAGTACAGCACGGGAGGTCGGACGACATTGTTAGGGATTAGCAAGCGGGGCAACAAAAAGATCCGAACTTTGTTGGTTCA
GTGTGCCAGGGTATTCATACAAAAACTGGAACACCAGTCTGGCAAGTTGGCCGACTGGGTCAGGGAGTTGTTGTGTCGGAAAAGCAACTTTGTCGTCACC
TGTGCTCTGGCAAACAAGCTGGCCAGAATAGCCTGGGCACTGACGGCGCGACAGCAAACTTACGAAGCATAAAGGCAGAAATACACCAGTTTAAACAATC
ATTCATCTGGTTTTGCGAATACTGATATTGATGATACTAACGGCCCACCGGCCTGTTGAGGAACCTGTAAAACGGAAAGGCTCATTGAAGCCGTATATTT
TCTGGAGGTTCATCAGGCGCGGAACTCATCGAGGCGCGGGAATAAAATCCCATTCAGACGCCGGATAGATTCAAGCAAGCCAATTTATCGTCAAAATCGG
TGTTGCAAAAACGGGAGTGACCATAGA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1005 bp | 334 aa | 68 | 1072 | + | No |
Chemistry : DEDD
ORF sequence :
MENIALIGIDLGKNSFHIHCQDHRGKAVYRKKFTRPKLIEFLATCPATTIAMEACGGSHFMARKLAELGHFPKLISPQFVRPFVKSNKNDFVDAEAICEA
ASRPSMRFVQPRTESQQAMRALHRVRESLVQDKVKTTNQMHAFLLEFGISVPRGAAVISRLSTVLEDSTLPLYLSQLLLKLQQHYHYLVEQIKDLESQLK
RKLDEDEVGQRLLSIPCVGTLTASTISTEIGDGKQYASSRDFAAATGLVPRQYSTGGRTTLLGISKRGNKKIRTLLVQCARVFIQKLEHQSGKLADWVRE
LLCRKSNFVVTCALANKLARIAWALTARQQTYEA
ASRPSMRFVQPRTESQQAMRALHRVRESLVQDKVKTTNQMHAFLLEFGISVPRGAAVISRLSTVLEDSTLPLYLSQLLLKLQQHYHYLVEQIKDLESQLK
RKLDEDEVGQRLLSIPCVGTLTASTISTEIGDGKQYASSRDFAAATGLVPRQYSTGGRTTLLGISKRGNKKIRTLLVQCARVFIQKLEHQSGKLADWVRE
LLCRKSNFVVTCALANKLARIAWALTARQQTYEA
Blast result :
Comments
The IR of this IS are not at its termini. In the IS sequence as given 7 nt separate IRl from the left-hand end of the element and 3 nt separate IRr from the right-hand end. The first residue of the sequence may in fact belong as the final residue, giving 6 nt on the left and 4 on the right.
Found in Tn5075.
All known copies are found in the same orientation in a 38 bp TIR of a Tn21-family transposon. They are inserted on one side of base 22 from the outer end of the Tn.
Sequenced copies of Tn5075 vary at up to 10 positions.
IS5075 is 93% identical to IS4321L and the transposase proteins are 98.5% identical.
IS5075 has been shown to exist in a circular form in which a -10 region created by the abutted terminal sequences and a -35 region located just inside the right-hand end of the element are correctly spaced to form a promoter.
Found in Tn5075.
All known copies are found in the same orientation in a 38 bp TIR of a Tn21-family transposon. They are inserted on one side of base 22 from the outer end of the Tn.
Sequenced copies of Tn5075 vary at up to 10 positions.
IS5075 is 93% identical to IS4321L and the transposase proteins are 98.5% identical.
IS5075 has been shown to exist in a circular form in which a -10 region created by the abutted terminal sequences and a -35 region located just inside the right-hand end of the element are correctly spaced to form a promoter.
References
1] Essa et al. (2003) Antimicrob. Agents Chemother. 47, 1115-1119
2] Partridge and Hall (2003) J. Bacteriol. 185, 6371-6384
3] Wain et al. (2003) Antimicrob. Agents Chemother. 47, 2732-2739.
2] Partridge and Hall (2003) J. Bacteriol. 185, 6371-6384
3] Wain et al. (2003) Antimicrob. Agents Chemother. 47, 2732-2739.