ISfinder Mirror Site

IS427

Family IS5
Group IS427

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
M55562	Y	Agrobacterium tumefaciens	Agrobacterium tumefaciens T37 plasmid pTiT37

DNA section

IS Length : 1271 bp

Ends

IR Length : 13/16

IRL : GGCCGTGAACCCATAAACTGGGTACCGGATTACTGATTGTCTCGACCGAT
IRR : GGCCGTAGACTCATAAGCCCGCAGCCACAGGCGCATTGACGCCAACTGGA

Insertion site

Left flank	Direct repeat	Right flank	DR Length
TGATTGCATC	TA	ACACAGTAAC	2
CGATTCATTC	TA	GTGAAGGCGA	2
CGCGGAATTT	TA	GATTGCTGAG	2

DNA sequence

GGCCGTGAACCCATAAACTGGGTACCGGATTACTGATTGTCTCGACCGATCAAATCCAGCGCTACATCGAAGGATGGCGCTATCGGTCTGAGGCTTTCTT
CCAGGTCGACGTTGGTCATGTCGAAGGCGACGAGGGGATATGGCGCATTTTCCCGCAAGTCGAATGCCACGGCTTCGCCGCCACCGTTGCCGCCGATCAC
GAACAAACCCCGGAAAAACTCTCGGAAAGTCCCGTCCCGTTCAATCTGAGCAGCTTCCTCGGCGGGATAGAGTTGGAACCAAAGCGGCTGAACGCTAGCG
GTCCTTCCCCGCCATTGCTGAAAGTCAGCAATGCGTAATAACTCTCAGGAAGATCGACCGGAGCCAGCGATTTGAGCGCGGCGATTGCGTCTGCGGAGGC
TCCATCGACCTTATGCCATTCTCGATTTTGCAGCACGCGGCCTCCACTTTCGCTTTCCTGAATGATGACGGCTTCCATTACAGATCAAAAGCTGATTCAG
CGCCAGCATGAGCCGATATGACCTGACCGATTTTGAATGGCGCGTGATTGAGCCACTTTTACCCAACAAGCCAAGAGGGGTGCCGCGCGTCGATGACCGT
CGCGTGCTGAATGGAATCTTCTGGGTGCTCCGATCCGGTGCGCCGTGGCGCGACCTGCCCGAACGCTATGGTCCGCGAACCACCTGCTACAACCGATTCA
TACGATGGCGGAAAGCAGGCGTCTGGGACCGAATGATGGACGCCATCACTGCTGCCTATGATGGCGACATCCAGATGATCGACAGCACTTCCGTTCGCGC
CCACCAGCAGGCTGCGACGGCAAAAAGGGGGATCGAGATCATTGTCTCGGCCGTTCCCGAGGCGGGCTCACGACCAAAATCCACGCGGTCGTCGATGGGC
AGGGTCTCCCGATCAGGCTGAGCCTAACCGCTGGGCAAAGCCACGACGGGCAAGCGGCTGACGGTTTGCTTGACCATGTGGGTGCCGGAACCATCGTGCT
CGCTGACAAGGCCTATGATGCCGATCGCATCCGGGCGTCTCTCCGCGAGAAGGGGGCGTTCGCCAATATTCCGCCCAAGGCAAACCGTAGGTCAAAACCG
TACTTCAGCACATGGCTATATCGCGAACGGAACCTGATCGAGCGGTTCTTCTCCAAGCTGAAGCATTTCCGTCGGGTCGCTACCCGCTACGACAAGCTGG
CGGAAAACTTCCTCGCCATGGTCCAGTTGGCGTCAATGCGCCTGTGGCTGCGGGCTTATGAGTCTACGGCC

Recoding section

Recoding by frameshift
Frame
Type
Experimentally demonstrated

Stimulators :

Shine-Dalgarno sequence :
Secondary structure :

Recoding motif :

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
414 bp	137 aa	508	921	+	No

ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MSRYDLTDFEWRVIEPLLPNKPRGVPRVDDRRVLNGIFWVLRSGAPWRDLPERYGPRTTCYNRFIRWRKAGVWDRMMDAITAAYDGDIQMIDSTSVRAHQ
QAATAKRGIEIIVSAVPEAGSRPKSTRSSMGRVSRSG

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
510 bp	170 aa	762	1271	+	No

ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

WRHPDDRQHFRSRPPAGCDGKKGDRDHCLGRSRGGLTTKIHAVVDGQGLPIRLSLTAGQSHDGQAADGLLDHVGAGTIVLADKAYDADRIRASLREKGAF
ANIPPKANRRSKPYFSTWLYRERNLIERFFSKLKHFRRVATRYDKLAENFLAMVQLASMRLWLRAYESTA

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
764 bp	255 aa	508	1271	+	Yes

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSRYDLTDFEWRVIEPLLPNKPRGVPRVDDRRVLNGIFWVLRSGAPWRDLPERYGPRTTCYNRFIRWRKAGVWDRMMDAITAAYDGDIQMIDSTSVRAHQ
QAATAKKGDRDHCLGRSRGGLTTKIHAVVDGQGLPIRLSLTAGQSHDGQAADGLLDHVGAGTIVLADKAYDADRIRASLREKGAFANIPPKANRRSKPYF
STWLYRERNLIERFFSKLKHFRRVATRYDKLAENFLAMVQLASMRLWLRAYESTA

Blast result :

Comments

The proposed IS427 putative transposase is a fusion between (508-825) and (825-1271). There is no stop codon at the end of the element ! Also, the first 508 bp are not coding for any protein sharing similarity with those of the other IS5 family members. All these assumptions remain to be confirmed. Also, note that at position 821, the sequence 5'-CAAAAAGGGGGA-3' is a potential site for a -1 frameshifting between both ORFs.
The STOP codon is in the DR.
IS427 is 82% aa similar to IS869.

References

1] De Meirsman, C., Croes, C., Desair, J., Verreth, C., Van Gool, A., and Vandeleyden, J. (1989) Plasmid 21, 129-137.
2] De Meirsman, C., van Soom, C., Verreth, C., Van Gool, A., and Vandeleyden, J. (1990) Plasmid 24, 227-234.
3] De Meirsman, C. (1990) Ph.D. Thesis, K.U.L., Leuven, 138 pp
4] De Meirsman, C., Vanderleyden, J., and Van Gool, A.P. (1990) in Zbarsky, I.B., and Harris, J.R. (Eds) Plenum Press, London pp. x-y.