ISfinder Mirror Site

IS220

Family IS1380
Group

MGE type ISRelated element(s) :
Isoform ISMsm12Synonym(s)

Accession number	Transposition	Origin	Host
AJ315500	ND	Mycobacterium fortuitum	Mycobacterium fortuitum

DNA section

IS Length : 3257 bp

Ends

IR Length : 15

IRL : CCTCGATCCACCGACTGTTGTGGATCGAGTGCGGTTCCCGCTGAAGAAAT
IRR : CCTCGATCCACCGACGGGTTCTGTGTCTGCGGCTGTGGCGGCTGTGGCTA

Insertion site

Left flank	Direct repeat	Right flank	DR Length
NNNNNNNNNG	CTAAG	NNNNNNNNN	5

DNA sequence

CCTCGATCCACCGACTGTTGTGGATCGAGTGCGGTTCCCGCTGAAGAAATGCTGGCCCTGATCTGGGCTGATTCGTACTTGGGCTGGTTGTGCCGGTCGC
TGCGGTTCGGCACTGCGAGTGCCCGTGGGTTGTACGGCCCCGGTGCAATCCGCGCTTAGCGACTCGTGGGCAGCGAATCCACGTGAAGTCGCAAGATCTT
GGCGATGGTGGTCGGCTTTCGGGGGCCGTGCGCTGTCACAGCGTCGAGGGCGAGGCCGGACAGAAGTGACGCTAGGCGTTGGGCTTCCAGGCTGGGCTGT
GGCACCGACATGCCCTTCAGGGCTGTGACCAGCACGTCATCCAGGTCGGTGGCCATTTGCGCTGTCGCTGCCCGGAATGCTTCAGTAGTTCGGGAGGCAG
CGATGACTTCCATGAGGATCACTGCCTCGCTATGCCGTTCGGCGTCAAGGGGAACGAGTTCTTCAAGTAGTGCGATCAGATGTTGTCGTTGTCGCGAGAG
GTCGCGCGATTGCGGTGGTGTGTGTTTCATCAGCCGCTGGCCCATGCGGTTTCCGGCCTCGGTAACTACTGCGACGACGAGGGATTCGTGATCTGCGAAG
TAGTGCCGGACTGACCCGATGTTCAACTGCGATTCCGCCGCAACACGGCGGAACGTCGCGGCGGACAGCCCCTCCTTCAGCACCAGACGTAGCGCGGCCT
CGACGATGTGACTTCGGCGTTCCACGGGGTCGATACGTGCTGGCATCGTCAAAGTCTAACGACAGCAGACGGCGGCCTATCACGGGTGTGATAAAAAGAA
TGCACGGTTGGCGGGCAGTCGCCGAGCAGACGGAGAGTACCTCGATGAATCATGTTGATGCGCTGGCCTGTAACCGAGAGAACTGGGACGAACGCGCAGA
TATCCATGCCCGGTCGCAGATGTATGACGTGGAGGGTTTCCTGGCGGACCGGTCCTTGATTTCGTCGGTGGTCCGAAATGACCTGACCGTGCTGCAACCG
CACCTGCCCTCCTCGAAGATCAGCGGTCAATCCCTGCTGCATCTGCAATGTCACATCGGGACCGATACCGTGTCATGGGCGCGGCTGGGCGCCGTCAACG
TCACCGGCTTGGATCTGTCGCCGAACTCACTACGTCACGCTCGACGCATCGCCGAGCAAGACGGGCAGGTGATCACATGGGTACAGGGCGATGCCCGGGT
CGCTTTCTTACTGATCGACGAGCAGTTCGATGTTGTTGTCACCAGCGCAGGAACCATCGTGTGGCTGCCAGAGCTGTCGGCGTGGGCGCGCTCCATCCAT
GACCTGCTGACACCCGGCGGTGTTTTCATGATTCGCGACGACCATCCGATATTGGCGGCGATGGAGTTCCAGCCGTGGACGATCAGCGATGACTACCTCT
CTGGTGGCGGTACCCGGACCTATGACGACGCCAGCACCTACACCGAGAACACCGACGTGGTGATCCGTCAGACAACGAATTACGAGTGGCGCCACGATCT
CAGTGAAGTCCTCACCGCGCTCTTGGAAGCAGACCTGCGTATCGAGGCCGTCCACGAGCTTGCTTACATGGATTGGCCAGCATTCCCGGCGTTGATTCCC
GATCCACGCGGTTGGACATTGCCCGCAGATGCGCCACGCATTCCGTTGAACTTCGCGATCGTCGCCCGCAGAGCCAGCTAAGTTCAACACGGGCACAGCG
ACTTCGAGCGCCGCGGATGGAACTATCGCACAGGTTTGCCGCGTCGTCGGCGGTCTTCGACGATGATCATCTCGTGTCAATCGCCGGTTTGGTGCCCGTG
ATGACGCTGGCCACCCAGACCGGTTTGTCGGCGCTATTGGCCGACAAGGTTCGGATCAGCGAACCGAGGATCAAGTCCGGTTCGGCCAACCCGTCACCGA
AGTTGACTACGCTGATCGCCGGGATGTGCGCCGGTGCCGACAGCATCGACGACCTCGACATCGTGCGCTCGGGCGGGATGAAGACCCTCTTCGACGACGT
GTACGCACCCTCAACCATCGGCACACTGTTGCGTGAGTTCACTTTCGGGCACGCCCGACAACTCGAAGCAGTACTTCGGGCTCACCTGGCCGAACTATGC
CAGCGAGCCGACCTGCTGCCAGGCATCGACGGGCGGACGTTCGTCGACATCGACTCATTGCTTCGCCCCGGTCTACGGCCACGCCAAACAGGGCGCCTCC
TACGGGCATACCAAGATCGCGGGAAAACAAATCCTGCGTAAAGGCCTGTCGCCGTTGATCACCACCATCAGCTCTAGTACGAGCGCTCCGGTGATCGCCG
GCGCACGGCTACGAGCAGGCAAGACCAACTCCGGCAAGGGCGCGGCCCGGATGATTGCCCAAGCGGTCGCGACCGCGCGCGCCGCCGGAGTCACCGGGCC
GATCTTGGTGCGCGGCGACTCTGCCTACGGCAACAGCACCGTGGCCGCGGCCTGTCGTCGGGCCGGCGCCCAGTTCTCGCTGGTGCTGACCAAGACGCCC
GCCGTCACTGCGGCCATTGATGCCATCAGTGACGGTGCCTGGATCCCGGTGAACTACCCCGGCGCAGTGCGCGACCCCGACACCGGCGCCTGGATCTCCG
ACGCCGAGGTCGCCGAAACCACCTACACCGCTTTCAGTTCCACCAAGACCCCCATCACTGCACGCTTGATCGTGCGCCGGGTCAAAGACGCCAGATTCCT
CGACGCACTGTTCCCGGTGTGGCGCTATCACCCCTTCTTCACCGACTCCGACGAGCCTGTCGACGCCGCCGACATCACCCATCGCCGCCACGCCATCATC
GAAACCGTCTTCGCCGACCTCATCGACGGACCCCTAGCCCACATGCCCTCAGGACGTTTCGGCGCGAACTCGGCCTGGATCCTCTGCGCGGCGATCGCCC
ACAACCTACTGCGCGCCGCAGGCGTCCTGGCCGGCACCGCCAACGCGGTCGCACGGGGATCCACATTGCGGCGACGCATCGTTACAGTTCCGGCCCGGCT
CGCCCGACCCCAGCGCCGACCCGTCCTGCATCTACCCACGCACTGGCCGTGGACAGATCAATGGCTCATGCTGTGGCGTAACACCATCGGATACAGCCCA
CCCGCGACCCCCTGCCACTGACTATCCCGCCGAAAGGCCCCGACCGGAGCACATAGGAAAAGCTGGACAGACCAGCCAGTACTGCCTGCCCGCGACCAGA
TACCGCCTAGCCACAGCCGCCACAGCCGCAGACACAGAACCCGTCGGTGGATCGAGG

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
591 bp	196 aa	746	156	-	No

ORF function : Passenger Gene
Annotation : Transcriptional regulator, TetR familyDescription : Transcriptional Regulator factor

ORF sequence :

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
882 bp	293 aa	800	1681	+	No

ORF function : Passenger Gene
Annotation : MethyltransferaseDescription :

ORF sequence :

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1407 bp	467 aa	1717	3123	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

Blast result :

Comments

The two first ORF are passenger genes annotated as transcriptional regulator, TetR family and methyltransferase.
There is unexpected frameshift in the transposase (ORF3), this is a pseudogene. The DNA sequence share 99% of identity with ISMsm12.

References

1] Waskar,M., Kumar,D., Kumar,A. and Srivastava,R.(2000)Microbiology, 146 (Pt 5), 1157-1162
2] Srivastava,R.(2001) Direct submission GenBank