IS1542
- Family IS256
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AF114715 | ND | Enterococcus faecium | Enterococcus faecium |
DNA section
IS Length : 1324 bp
Ends
IR Length : 20/27
IRL : GATAAAGTCCATATAATTGTGTAAAAGATAAAAGGCCATGTAACAGCCCT
IRR : AGTCAAGTCCATAATCCTGTGTAAAATACTATACAATGTTTTACCGGTCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
GATAAAGTCCATATAATTGTGTAAAAGATAAAAGGCCATGTAACAGCCCTTTTACGGTACAATGTTTTTAACCACAAAAACATACCCAGGAGGACGTTAC
ATGACCCAAGTACATTTTACACTGAACAACGAAGAGGTTCAAAGTATTATTGAACATTCGGTAAAAGATGATGTTTCTAAAAATATTTTAACCACTGTTT
TCAACCAATTGATGGAAAATCAACGAACAGAATATATTCAAGCCGATGACTATGAACGTTCAGAAAGTCGTCAGAGTCAAAGAAATGGCTATTATGAGCG
AGACTTTACGACTCGTGTGGGTACACTCGAATTAAAAGTGCCTAGAACACGTGATGGTGAATTTTCACCGACGGTGTTTGAGCGTTATCAGCGAAATGAA
AAGGCACTGCTCGCTTCAATGCTTGAGATGTATGTTTCGGGCGTTTCGACACGTAAAGTTTCAAAGATTGTTGAAGAGCTATGTGGAAAATCTGTATCGA
AATCTTTTGTTTCTAGCCTGACTGAGCAGTTAGACCCGATGGTCAACGAATGGCAGAACCGTTCACTCTCAGGTACGAATTATCCTTATCTGATGACTGA
TGTTCTCTACATAAAAGTCCGTGAGGACCATCGAGTGCTTTCTAAAAGCTGCCATATTGCGATCGGGATAACAGAAGGTGGCGACCGTGAAATCATTGGC
TTCATGATTCAAAATGAAGAAAGTGATGACACATGGTCCATCTTCTTTGAATACTTAAAAGAACGCGGCCTAAAGGGGACAGAACTCATCATTTCTGATG
CCCATAAAGGCCTAGTGTCTGCGATTCGTAAGTCATTTACCAACGCAAGTTGGCAGAGATGCCAGGTCCATTTTTTAAGAAACATCTTCAGTTCCATTCC
AAAAAAGAATTCAAAACCGTTTAGAGAAGCAGTAAAAGCGATCTTTAAGTTTACGGATATTGAACTCGCTCGAACAGCTAAGAATGCCTTAGTCGGTGAA
TATATCGACCAGCCTAAATATACAAAAGCCTGCGAAATATTGGATAATGGCTTCGAAGATGCCTTTCAATACACGGTTATCGGAAATAGTCATAATCGGC
TAAAAAGCACCAACCTTCTTGAACGACTGAACCAGGAAGTCCGCAGAAGAGAAAAGATTATTCGGATTTTTCCCAACCGAACGTCTGCCAATCGATTAAT
TGGAGCTGTCCTTATGGACCTTCATGACGAATGGCTCAGTTCTACAAGAAAATATATTAAGTTTGATCAATGAGAGACCGGTAAAACATTGTATAGTATT
TTACACAGGATTATGGACTTGACT
ATGACCCAAGTACATTTTACACTGAACAACGAAGAGGTTCAAAGTATTATTGAACATTCGGTAAAAGATGATGTTTCTAAAAATATTTTAACCACTGTTT
TCAACCAATTGATGGAAAATCAACGAACAGAATATATTCAAGCCGATGACTATGAACGTTCAGAAAGTCGTCAGAGTCAAAGAAATGGCTATTATGAGCG
AGACTTTACGACTCGTGTGGGTACACTCGAATTAAAAGTGCCTAGAACACGTGATGGTGAATTTTCACCGACGGTGTTTGAGCGTTATCAGCGAAATGAA
AAGGCACTGCTCGCTTCAATGCTTGAGATGTATGTTTCGGGCGTTTCGACACGTAAAGTTTCAAAGATTGTTGAAGAGCTATGTGGAAAATCTGTATCGA
AATCTTTTGTTTCTAGCCTGACTGAGCAGTTAGACCCGATGGTCAACGAATGGCAGAACCGTTCACTCTCAGGTACGAATTATCCTTATCTGATGACTGA
TGTTCTCTACATAAAAGTCCGTGAGGACCATCGAGTGCTTTCTAAAAGCTGCCATATTGCGATCGGGATAACAGAAGGTGGCGACCGTGAAATCATTGGC
TTCATGATTCAAAATGAAGAAAGTGATGACACATGGTCCATCTTCTTTGAATACTTAAAAGAACGCGGCCTAAAGGGGACAGAACTCATCATTTCTGATG
CCCATAAAGGCCTAGTGTCTGCGATTCGTAAGTCATTTACCAACGCAAGTTGGCAGAGATGCCAGGTCCATTTTTTAAGAAACATCTTCAGTTCCATTCC
AAAAAAGAATTCAAAACCGTTTAGAGAAGCAGTAAAAGCGATCTTTAAGTTTACGGATATTGAACTCGCTCGAACAGCTAAGAATGCCTTAGTCGGTGAA
TATATCGACCAGCCTAAATATACAAAAGCCTGCGAAATATTGGATAATGGCTTCGAAGATGCCTTTCAATACACGGTTATCGGAAATAGTCATAATCGGC
TAAAAAGCACCAACCTTCTTGAACGACTGAACCAGGAAGTCCGCAGAAGAGAAAAGATTATTCGGATTTTTCCCAACCGAACGTCTGCCAATCGATTAAT
TGGAGCTGTCCTTATGGACCTTCATGACGAATGGCTCAGTTCTACAAGAAAATATATTAAGTTTGATCAATGAGAGACCGGTAAAACATTGTATAGTATT
TTACACAGGATTATGGACTTGACT
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1173 bp | 390 aa | 101 | 1273 | + | No |
Chemistry : DDE
ORF sequence :
MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTEYIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNE
KALLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTNYPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIG
FMIQNEESDDTWSIFFEYLKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFREAVKAIFKFTDIELARTAKNALVGE
YIDQPKYTKACEILDNGFEDAFQYTVIGNSHNRLKSTNLLERLNQEVRRREKIIRIFPNRTSANRLIGAVLMDLHDEWLSSTRKYIKFDQ
KALLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTNYPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIG
FMIQNEESDDTWSIFFEYLKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFREAVKAIFKFTDIELARTAKNALVGE
YIDQPKYTKACEILDNGFEDAFQYTVIGNSHNRLKSTNLLERLNQEVRRREKIIRIFPNRTSANRLIGAVLMDLHDEWLSSTRKYIKFDQ
Blast result :
Comments
The exact ends of this element remain to be defined.
IS1542 is 81 % homologous to IS256 and was detected in the genomes of 26/28 VanA Enterococci isolated from poultry in the UK and Ireland but in only 2/65 glycopeptide sensitive or VanC enterococci from hospital patients in the UK and Ireland.
IS1542 is 81 % homologous to IS256 and was detected in the genomes of 26/28 VanA Enterococci isolated from poultry in the UK and Ireland but in only 2/65 glycopeptide sensitive or VanC enterococci from hospital patients in the UK and Ireland.
References
1] Darini, A.L.C., Palepou, M.-F. I. and Woodford, N. (1999) FEMS Microbiology Letters. 173, 341-346.