IS1471
- Family IS630
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| U67938 | ND | Burkholderia cepacia | Burkholderia cepacia 2a plasmid pIJB1 |
DNA section
IS Length : 1113 bp
Ends
IR Length : 18/25
IRL : TAGATGAGGCCCAATAAAGTATGAATTTTCTCTGCGTTTTCTGGTCAGAG
IRR : TTGATCCGGCCCTGTGAAGTATTAAGCCGCGTAGCGAACGTAACGATCTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TGGACGTTCA | TA | AGCACCTGGC | 2 |
DNA sequence
TAGATGAGGCCCAATAAAGTATGAATTTTCTCTGCGTTTTCTGGTCAGAGATTGATGGAAAAAGAAAACGCAAGAAAGCAAACGCTGGAGCAACTTCACG
AGCGCCGCAAGCAAGTCGTTCGGCTGCACAAGAAGGGCATCAAGATCATGCAAATCGTGGAGATGACAGGGCTGAGCTACCCGCCAGTGCGCGCCACGAT
TGATCTGTTTGAAGCTGGTGGCTGGGGCGCCATCCGACCAGCCTTGCGAGGGCGCAGCCCAGGGGATGGCCGTGTGCTCAGCCCAGCGCAAGAAGGGACG
ATTCAGCGGATGATTATTGACAAGCGGCCCGAGCAACTCAAGATGGACTTTCATCTGTGGAGCCGAGCTGCCGTGAGCCAATTGATCGAGCAAGAATTTG
GCGTAAAGCTACAGACTCGAAGTACCGGAAAGTACCTCACCCGCTGGGGCTTCACGCCGCAAAAGCCCATCAAGCGAGCTTATGAGCAAAGCCCTGCCGC
AGTACAAGCTTGGTTGGAGGGCGAGTATCCCGGTATCGAGCAGCGCGCCAGAGCTGAAGGGGCGGAAATTCACTGGGGCGATGAGACGGCGTTGGTTAAC
ACCGACGTGCGCGGCAGAAGCTATGCGCCGACAGGCAAAACTCCGGTGACCATGGCTATCGGTGGCACGCGTCAGAAGCTATCGATGATTGCAACAGTGA
CCAACCAGGGCAAGACACGCTGGATGATCATTGATGAAGCATTTGACGCCGACAAGCTCATTGAATTCCTGCAAGCCTTGATCAAGGATGCCGGGAAAAA
AGTCTTTTTGATTCTGGACAATTTGCGCGTGCATCACAGCAAACTTGTGAAGGCTTGGGTGGCCGAACATCAAGACCAGATTGAGCTGTTTTACTTACCC
AGCTACAGCCCCCAGCTCAATCCAGAGGAGCGTCTGAATGCGGATCTCAAGCAAGAGATAGGCAAGCGAGTGCCGGTAAGAACCAAGGCCAAGTTACGCG
AGGCTGCCAATGATCACATGGCGATGCTGGAGCAAAACCCTGAGCGCGTTATTGGCTACTTCCAAGATCGTTACGTTCGCTACGCGGCTTAATACTTCAC
AGGGCCGGATCAA
AGCGCCGCAAGCAAGTCGTTCGGCTGCACAAGAAGGGCATCAAGATCATGCAAATCGTGGAGATGACAGGGCTGAGCTACCCGCCAGTGCGCGCCACGAT
TGATCTGTTTGAAGCTGGTGGCTGGGGCGCCATCCGACCAGCCTTGCGAGGGCGCAGCCCAGGGGATGGCCGTGTGCTCAGCCCAGCGCAAGAAGGGACG
ATTCAGCGGATGATTATTGACAAGCGGCCCGAGCAACTCAAGATGGACTTTCATCTGTGGAGCCGAGCTGCCGTGAGCCAATTGATCGAGCAAGAATTTG
GCGTAAAGCTACAGACTCGAAGTACCGGAAAGTACCTCACCCGCTGGGGCTTCACGCCGCAAAAGCCCATCAAGCGAGCTTATGAGCAAAGCCCTGCCGC
AGTACAAGCTTGGTTGGAGGGCGAGTATCCCGGTATCGAGCAGCGCGCCAGAGCTGAAGGGGCGGAAATTCACTGGGGCGATGAGACGGCGTTGGTTAAC
ACCGACGTGCGCGGCAGAAGCTATGCGCCGACAGGCAAAACTCCGGTGACCATGGCTATCGGTGGCACGCGTCAGAAGCTATCGATGATTGCAACAGTGA
CCAACCAGGGCAAGACACGCTGGATGATCATTGATGAAGCATTTGACGCCGACAAGCTCATTGAATTCCTGCAAGCCTTGATCAAGGATGCCGGGAAAAA
AGTCTTTTTGATTCTGGACAATTTGCGCGTGCATCACAGCAAACTTGTGAAGGCTTGGGTGGCCGAACATCAAGACCAGATTGAGCTGTTTTACTTACCC
AGCTACAGCCCCCAGCTCAATCCAGAGGAGCGTCTGAATGCGGATCTCAAGCAAGAGATAGGCAAGCGAGTGCCGGTAAGAACCAAGGCCAAGTTACGCG
AGGCTGCCAATGATCACATGGCGATGCTGGAGCAAAACCCTGAGCGCGTTATTGGCTACTTCCAAGATCGTTACGTTCGCTACGCGGCTTAATACTTCAC
AGGGCCGGATCAA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1038 bp | 345 aa | 55 | 1092 | + | No |
Chemistry : DDE
ORF sequence :
MEKENARKQTLEQLHERRKQVVRLHKKGIKIMQIVEMTGLSYPPVRATIDLFEAGGWGAIRPALRGRSPGDGRVLSPAQEGTIQRMIIDKRPEQLKMDFH
LWSRAAVSQLIEQEFGVKLQTRSTGKYLTRWGFTPQKPIKRAYEQSPAAVQAWLEGEYPGIEQRARAEGAEIHWGDETALVNTDVRGRSYAPTGKTPVTM
AIGGTRQKLSMIATVTNQGKTRWMIIDEAFDADKLIEFLQALIKDAGKKVFLILDNLRVHHSKLVKAWVAEHQDQIELFYLPSYSPQLNPEERLNADLKQ
EIGKRVPVRTKAKLREAANDHMAMLEQNPERVIGYFQDRYVRYAA
LWSRAAVSQLIEQEFGVKLQTRSTGKYLTRWGFTPQKPIKRAYEQSPAAVQAWLEGEYPGIEQRARAEGAEIHWGDETALVNTDVRGRSYAPTGKTPVTM
AIGGTRQKLSMIATVTNQGKTRWMIIDEAFDADKLIEFLQALIKDAGKKVFLILDNLRVHHSKLVKAWVAEHQDQIELFYLPSYSPQLNPEERLNADLKQ
EIGKRVPVRTKAKLREAANDHMAMLEQNPERVIGYFQDRYVRYAA
Blast result :
Comments
The 102-kb pIJB1 plasmid from Burkholderia cepacia 2a contains the genes involved in 2,4-dichlorophenoxyacetate (2,4-D) degradation. This plasmid carries an IS1471 element inserted into a copy of IS1071 (Xia et al., 1996). Note that the proposed IS1471 ends have been modified, assuming that the element, like the other IS630 family members, has duplicated a "TA" target. This assumption remains to be confirmed. IS1471 putative transposase is 26 % identical to that of ISRm2011-2 from Rhizobium meliloti (in a 322-aa overlap).
References
1] Xia, X.-S., Smith, A.R.W., and Bruce, I.J. (1996) FEMS Microbiol. Lett. 144, 203-206.