IS1411

  • Family ISL3
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
M57500 Y Pseudomonas sp.
Pseudomonas putida
Pseudomonas sp. EST1001
DNA section
IS Length : 1419 bp

Ends


IR Length : 24

IRL : GGCTCTTCGCATTTAAGGGTGTAGAAAAAATCTGACCGGCTGGAAAAGGA
IRR : GGCTCTTCGCATTTAAGGGTGTAGCTGATTTCTAAACCCTCCTGACTTCA

Insertion site


Left flankDirect repeatRight flankDR Length
GTCGTTGTGATGTATTCCNNNNNNNNNN8

DNA sequence

GGCTCTTCGCATTTAAGGGTGTAGAAAAAATCTGACCGGCTGGAAAAGGAGTCGAGGATCTTAGAAAATGTAAGCTCTCACACCAACAAAAACTAAGCCC
CCGACGTGAACAATCTTACCTTTTCTAGCCCTGATCTTTCCAGCTTTTGCCAACTGAATAACCTCGGCCTGACTGCCACTGGACAACATCTTTGTGCAGA
GCGCGCCGTCATCGAATGTCGTTTAACCAAAGCACCCGAGCCATGCCCCAAGTGCGGGGCTGCTGGTGTTTCACGCGGTACTGTCGATAGGCATCTTGCT
CACACACCTTACGGACAACGACCAACCAGATTGCTGCTGCGTATCCGTCGCTGGCGTTGTGCTTGCGGCTGTTTCTGGCATGAAGATACAAACAGTGCAG
CACCTCCACGTTCAAAGCTTTCATATGGGGCGATACGCTGGGCATTAGCTGCCATCGTGCTGGATCATCTGTCGGTATCTCGTGTTGCGAGCCAGCTTGA
TGTTGCATGGCACACCGCTAATAATGCCATTATCAACGAAGGACGGCGCCTGCTTTTTAATGACTCGACACGTTTTGACGGTGTGACCGTGCTGGGCGTG
GATGAGCATGTTTGGCGACATACACGCTGTGGTGACAAGTACGTCACCATCGTGGTTGACCTTACGCCCGTGCGTAACAAAAACGGGCCGGCCCGCTTGC
TCGATGTGCTGGAAGGCCGCTCCAAACAAGCCTTTAAGCAATGGCTACAGAGTCGCCCCAAATCGTGGCGTGACCAGATCGAAAGCATTGCCATGGACGG
TTTTACGGGGTTTAAATCTGCAGCGCAAGAAGCCCTGCCTCAAGCCCAAACCGTGCTGGATCCTTTCCATGTCGTGCGCTGGGCAAGCAACATGCTGGAT
GAATGCCGCCGGCGTGTGCAACACGACATCCTGGGCCGCAGAGGGCGTAAAAATGACCCGCTCTACAAAAGCCGTCGAACGCTACTGACTCGGATCAGCT
ACCTGTCTGACGCCAACAAAAAGCAACTGTTCCAGCTGTTTGCAGATGAGCACCACCTCGAAGTGGATTGCACCTGGAGCATGTACCAACGGGTGGTCAG
CGCCTACAACGAGCCGGATCGAAAACGCGGTAAAAAACTCATGGAAGAGGTCATAAATATCATTACAGTTAGCGACTTGCCCAAAGCGCTCATCGAGGTG
AAAGGCTTGGGGGAAACGCTGAAAAAATACGCTGAGAGCATCCTTGCCTACTTCGACCGGCCAGGAACCAGCAACGGTCCAACAGAAGCTATCAACGGGC
GACTTGAGCACTTACGAGGTACCGCCTTGGGCTTTAGAAATTTAACCAATTACATAGCCAGGTGCTTGCTGAAGTCAGGAGGGTTTAGAAATCAGCTACA
CCCTTAAATGCGAAGAGCC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1301 bp433 aa1071407+No
ORF function : Transposase
Chemistry : Unknow

ORF sequence :

MNNLTFSSPDLSSFCQLNNLGLTATGQHLCAERAVIECRLTKAPEPCPKCGAAGVSRGTVDRHLAHTPYGQRPTRLLLRIRRWRCACGCFWHEDTNSAAP
PRSKLSYGAIRWALAAIVLDHLSVSRVASQLDVAWHTANNAIINEGRRLLFNDSTRFDGVTVLGVDEHVWRHTRCGDKYVTIVVDLTPVRNKNGPARLLD
VLEGRSKQAFKQWLQSRPKSWRDQIESIAMDGFTGFKSAAQEALPQAQTVLDPFHVVRWASNMLDECRRRVQHDILGRRGRKNDPLYKSRRTLLTRISYL
SDANKKQLFQLFADEHHLEVDCTWSMYQRVVSAYNEPDRKRGKKLMEEVINIITVSDLPKALIEVKGLGETLKKYAESILAYFDRPGTSNGPTEAINGRL
EHLRGTALGFRNLTNYIARCLLKSGGFRNQLHP

 

Blast result :
Comments
IS1411 activates transcription of the pheBA operon by an outward directed promoter in the left end.IS1411 forms circular molecules which migrate at the expected position in agarose gel electrophoresis. One cloned junction showed 5bp from outside IRL separating the two ends.
References
1] Kallastu, A., Horak, R, and Kivisaar, M. (1998) J. Bact. 180, 5306-5312.