IS1380A
- Family IS1380
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| D90424 | Y | Acetobacter pasteurianus | Acetobacter xylinum NCIB11664 Acetobacter pasteurianus NC11380 Acetobacter pasteurianus NCIB7214 Acetobacter aceti subsp. aceti 1023 Acetobacter aceti subsp. aceti IFO3284 Acetobacter polyoxogenes NCI1028 |
DNA section
IS Length : 1665 bp
Ends
IR Length : 13/15
IRL : CGCGGAAATCTCACAGAAGGGTTGTACATCGGGTTAGATTATGAAAAAGT
IRR : CGCGGATTTCTCACACTTGAGGCAATTTCGGGTCATTTTGTAGAATTCAG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| NNNNNNNNNN | TCGA | NNNNNNNNNN | 4 |
| NNNNNNNNNN | TCGA | NNNNNNNNNN | 4 |
| NNNNNNNNNN | TCGA | NNNNNNNNNN | 4 |
| NNNNNNNNNN | TCGG | NNNNNNNNNN | 4 |
| NNNNNNNNNN | CTGA | NNNNNNNNNN | 4 |
DNA sequence
CGCGGAAATCTCACAGAAGGGTTGTACATCGGGTTAGATTATGAAAAAGTCTATTTTGTGTAAAAGAAATTTTCGTAAGCTATAAGAAAACCCGATGCAG
ACAGAGTGTAGCGCAGGCGCGTATGAGTTTCCAGCCTCCTGTGGACGGCGTGTTGTGGCCCGTTTTGACGGGGGTCGCATGAGTTCGGATGGGGGCGTCA
TTCTGGTGAAGCAGGCTGATGACATTCTGGGTCTCAGCCGCCGCTTTGCTGCCTGTTTTCGCGATAAGCGGCATCCCGGCTTTGTGGAATACCGGGTTGA
AGACCTTGTCCGTCAGCGGATCATGGGCCTGGCACTGGGCTATGAAGATCTCAATGATCACGATGCCCTGCGGCATGACCTGATCTTTGGTCTGGCCTCG
GGTCGTCTGTCAGGAGGCCGGGCAAACTGCGCAGCATTGGCTGGCAAATCTACACTGAACCGGTTGGAGCGCAGTGGGCACAAGGCAGATCGTTACTGTC
GGATCATTGCTGATCATGAGGCCCTGGCTACCCTGTTCGTCACGCTCTTCCTTGACCAGCATGAGCACGCACCCGCCCGGATCGTTCTGGATGTGGATGC
CACCGATGACCGTATCCATGGCCATCAGGAAGGCCGCGCCTTTCATGGATATTACGGCCATAACTGCTATCTTCCCCTGTATGTCTTCTGCGGGGACCAT
CTCCTCAGCGCTACCCTGCGCACGGCAGACAGGGACCCGGGGAAGGAAGCACTGGCAGACATCCGCCGGATCGTGGAGCAGATCAGGAGCCGTTGGCCCC
GGGTGCGTATCCTGGTGCGTGGGGACAGCGGTTTCGCCCGGGACAGTCTGATGACATGGTGCGAAGACAACCACGTTGACTTCCTGTTCGGGCTTGCAGG
CAACACCCGCCTGTATGACCGGATTGCCTCTTTGTCCGCTGAGGTTCGTGACGAAGCCGCCACGACAGGCAGAGCTGCGCGCGGTTTCGCCTCCTTTGAC
TGGATCACAAAGGACAGCTGGACGCGCCGCAGGCGGGTCGTGGCCAAGGCCGAATGGCGCCACGGCAACCGCTATCATCGCTTCATTGTCACCACCCTAC
CGCAGGGAATGTCCGACCCCCGCCATCTCTACGAACAGATTTACTGCGCACGCGGGGATATGGAAAACCGCATCAAGGAATGCCAGATGGATCTGTTCTC
AGACAGGACCTCGTCCCACACCATCCGGGCCAACCAGCTCCGGCTGTGGTTCTCGGCCGCAGCCTATGTCCTGCTGACCGCTCTGCAAAGACTGGCCCTT
GGCCAGACCAGCCTGGAGACGGCGACCTGTGGCACCATACGCGCACGGCTGCTCAAAATCGCGACACGTGTAACGCTCAGCGTCCGTCGGATTGTCCTGT
CCATGCCGGACATGTTCCCCTGTCAGCATGAATTCGCCCTCGCTCATGCACGATTGCGAAGGCTCCGGCAGGCCATCTGAAGAAACAGACAGTGCACAGA
CCACATAGCTTCCACCAACACTGCCTTCTCAGGCCGTGACACCCTCACTGCGTTCAGAACCCGCCGCCAGAAGCAGAATATCGTCAATATTCCAGATCGG
GCTCCTGTGGGATGACTGAATTCTACAAAATGACCCGAAATTGCCTCAAGTGTGAGAAATCCGCG
ACAGAGTGTAGCGCAGGCGCGTATGAGTTTCCAGCCTCCTGTGGACGGCGTGTTGTGGCCCGTTTTGACGGGGGTCGCATGAGTTCGGATGGGGGCGTCA
TTCTGGTGAAGCAGGCTGATGACATTCTGGGTCTCAGCCGCCGCTTTGCTGCCTGTTTTCGCGATAAGCGGCATCCCGGCTTTGTGGAATACCGGGTTGA
AGACCTTGTCCGTCAGCGGATCATGGGCCTGGCACTGGGCTATGAAGATCTCAATGATCACGATGCCCTGCGGCATGACCTGATCTTTGGTCTGGCCTCG
GGTCGTCTGTCAGGAGGCCGGGCAAACTGCGCAGCATTGGCTGGCAAATCTACACTGAACCGGTTGGAGCGCAGTGGGCACAAGGCAGATCGTTACTGTC
GGATCATTGCTGATCATGAGGCCCTGGCTACCCTGTTCGTCACGCTCTTCCTTGACCAGCATGAGCACGCACCCGCCCGGATCGTTCTGGATGTGGATGC
CACCGATGACCGTATCCATGGCCATCAGGAAGGCCGCGCCTTTCATGGATATTACGGCCATAACTGCTATCTTCCCCTGTATGTCTTCTGCGGGGACCAT
CTCCTCAGCGCTACCCTGCGCACGGCAGACAGGGACCCGGGGAAGGAAGCACTGGCAGACATCCGCCGGATCGTGGAGCAGATCAGGAGCCGTTGGCCCC
GGGTGCGTATCCTGGTGCGTGGGGACAGCGGTTTCGCCCGGGACAGTCTGATGACATGGTGCGAAGACAACCACGTTGACTTCCTGTTCGGGCTTGCAGG
CAACACCCGCCTGTATGACCGGATTGCCTCTTTGTCCGCTGAGGTTCGTGACGAAGCCGCCACGACAGGCAGAGCTGCGCGCGGTTTCGCCTCCTTTGAC
TGGATCACAAAGGACAGCTGGACGCGCCGCAGGCGGGTCGTGGCCAAGGCCGAATGGCGCCACGGCAACCGCTATCATCGCTTCATTGTCACCACCCTAC
CGCAGGGAATGTCCGACCCCCGCCATCTCTACGAACAGATTTACTGCGCACGCGGGGATATGGAAAACCGCATCAAGGAATGCCAGATGGATCTGTTCTC
AGACAGGACCTCGTCCCACACCATCCGGGCCAACCAGCTCCGGCTGTGGTTCTCGGCCGCAGCCTATGTCCTGCTGACCGCTCTGCAAAGACTGGCCCTT
GGCCAGACCAGCCTGGAGACGGCGACCTGTGGCACCATACGCGCACGGCTGCTCAAAATCGCGACACGTGTAACGCTCAGCGTCCGTCGGATTGTCCTGT
CCATGCCGGACATGTTCCCCTGTCAGCATGAATTCGCCCTCGCTCATGCACGATTGCGAAGGCTCCGGCAGGCCATCTGAAGAAACAGACAGTGCACAGA
CCACATAGCTTCCACCAACACTGCCTTCTCAGGCCGTGACACCCTCACTGCGTTCAGAACCCGCCGCCAGAAGCAGAATATCGTCAATATTCCAGATCGG
GCTCCTGTGGGATGACTGAATTCTACAAAATGACCCGAAATTGCCTCAAGTGTGAGAAATCCGCG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1386 bp | 461 aa | 95 | 1480 | + | No |
Chemistry : DDE
ORF sequence :
MQTECSAGAYEFPASCGRRVVARFDGGRMSSDGGVILVKQADDILGLSRRFAACFRDKRHPGFVEYRVEDLVRQRIMGLALGYEDLNDHDALRHDLIFGL
ASGRLSGGRANCAALAGKSTLNRLERSGHKADRYCRIIADHEALATLFVTLFLDQHEHAPARIVLDVDATDDRIHGHQEGRAFHGYYGHNCYLPLYVFCG
DHLLSATLRTADRDPGKEALADIRRIVEQIRSRWPRVRILVRGDSGFARDSLMTWCEDNHVDFLFGLAGNTRLYDRIASLSAEVRDEAATTGRAARGFAS
FDWITKDSWTRRRRVVAKAEWRHGNRYHRFIVTTLPQGMSDPRHLYEQIYCARGDMENRIKECQMDLFSDRTSSHTIRANQLRLWFSAAAYVLLTALQRL
ALGQTSLETATCGTIRARLLKIATRVTLSVRRIVLSMPDMFPCQHEFALAHARLRRLRQAI
ASGRLSGGRANCAALAGKSTLNRLERSGHKADRYCRIIADHEALATLFVTLFLDQHEHAPARIVLDVDATDDRIHGHQEGRAFHGYYGHNCYLPLYVFCG
DHLLSATLRTADRDPGKEALADIRRIVEQIRSRWPRVRILVRGDSGFARDSLMTWCEDNHVDFLFGLAGNTRLYDRIASLSAEVRDEAATTGRAARGFAS
FDWITKDSWTRRRRVVAKAEWRHGNRYHRFIVTTLPQGMSDPRHLYEQIYCARGDMENRIKECQMDLFSDRTSSHTIRANQLRLWFSAAAYVLLTALQRL
ALGQTSLETATCGTIRARLLKIATRVTLSVRRIVLSMPDMFPCQHEFALAHARLRRLRQAI
Blast result :
Comments
IS1380 activity is associated with the loss of ethanol-oxidizing ability (8/11 insertions into the cytochrome c gene), and genetic instability of Acetobacter pasteurianus. Its transposition might be induced by ethanol or acetic acid. There are about 100 IS1380 copies in the Acetobacter pasteurianus genome. Hybridization experiments have shown the presence of IS1380 in Acetobacter aceti subsp. aceti, Acetobacter pasteurianus, Acetobacter polyoxogenes, and Acetobacter xylinum .
References
1] Takemura, H., Horinouchi, S., and Beppu, T. (1991) J. Bacteriol. 173, 7070-7076.