IS1353
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| U40482 | ND | Pseudomonas aeruginosa | Pseudomonas aeruginosa |
DNA section
IS Length : 1613 bp
Ends
IR Length : 20/24
IRL : TGGGGTGCGGACAAAATCTTGGACTACTTTAGGAGTAGTTCATGTATTCG
IRR : TGGGGGTGCGGACGATTTCTTGGACGGTTTATACGGACATCAATCCGACC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
TGGGGTGCGGACAAAATCTTGGACTACTTTAGGAGTAGTTCATGTATTCGTATGAAGATCGCCTTCGAGCCGTGAGGTTGTACCTGAAGCTTGGGCGCCG
GATGAGCGCCACACTACGGCAGCTGGGATACCCCACCAAGAACTCGCTGAAGGCCTGGTTGGCAGAATTCGAACGGAATCAGGATCTTCGCCGAGGCTAT
CAACGGATAAAACGGCAGTACACCGATGAGCAAAAGCAACGGGCAGTAGATCACTATATCGAACAAGGCTACTGCCTGAGTCACACAATCCGAAGCCTGG
GCTACCCAAGCCGCGAGGCCTTGCGTGCCTGGATCCGTGATTTACGCCTGAATTCGCTAGGACGGTCGTCGGCAGCAGCGCTCCCACAGTCGCCCGCTCT
CGCCTCGAGAAGCAAGCAAGCCGTCATTGCACTGAACCTGCGCGTAGGTTCGGCAAAGGATGTGGCCGACACTGTCGGTGTATCGCGACCAACGTTGTAT
AACTGGCAGCATCGATTACTTGGCAAAGTGCCCCTAAAACCCATGACAAAGAAGAAAGGTGACACCTCGCTCGAGCAGCGGCATGAGGCACTACTCAGGG
AACTGGCCGAACTGGAGAGCCAGAACCAGCGGCTTCGCATGGAGAATGCAATTCTGGAGAAGGCGAGTGAATTGATAAAAAAGACATGGGCATCAACCCC
CTCGAACTGACAAGCCGAGAAAAAACGAAGGTGGTTGATGCCCTCAGAGTCACGTTTCCATTAGCCAATCTGTTGTGCGGCCTGAAGCTGGCGCGCAGCA
CATACTTCTATCAACGCCTGCGGCAGACGCGGCCCGACAAGTACACGCAGGTGCGTGAGGTCATTCGGACTATCTTCGAGGACAACTACCGCTGCTATGG
CTATCGACGCATTGATAGTGCCTTGCGCCTTGGTGGCATGCGTGTGTCCGAGAAGGTCGTGCGTCGCTTGATGGCGCAAGAGCGTCTGGTCGTGAGAACA
CCGCGCCGCCGGCGCTTCTCGGCGTATGCTGGCGACCCGACACCAGCGGTCCCGAATCTGCTGAATCGCGACTTTCACGCGTCGGCGCCGAATACGAAAT
GGTTGACCGATCTGACGGAAATACACATTCCGGCAGGGAAGGTCTACGTCTCGCCGATCGTCGATTGCTTCGATGGGCTGGTGGTGGCCTGGAATATCGG
CACCAGCCCGGATGCGAACCTGGTCAATACCATGCTGGATCACGCGGTACGGACACTGCGACCCGGTGAGCATCCGGTTATCCATTCGGACAGGGGCTCG
CATTATCGCTGGCCTGCGTGGATCCGCCGCACTGAAAATGCCCAATTAACGCGGTCGATGTCCAAAAAGGGCTGCTCGCCAGACAATGCTGCATGCGAGG
GCTTTTTCGGACGATTGAAGACCGAACTAATCTACCCGAGGAATTGGCAGCACGTGACGCTGAAAGACCTCATGACGCGAATCGATGCCTATATCCACTG
GTACAACGAGCGCCGCATCAAAGTGTCGCTTGGCGGGCGTAGTCCCATCGAGTATCGTCATGCGGTCGGATTGATGTCCGTATAAACCGTCCAAGAAATC
GTCCGCACCCCCA
GATGAGCGCCACACTACGGCAGCTGGGATACCCCACCAAGAACTCGCTGAAGGCCTGGTTGGCAGAATTCGAACGGAATCAGGATCTTCGCCGAGGCTAT
CAACGGATAAAACGGCAGTACACCGATGAGCAAAAGCAACGGGCAGTAGATCACTATATCGAACAAGGCTACTGCCTGAGTCACACAATCCGAAGCCTGG
GCTACCCAAGCCGCGAGGCCTTGCGTGCCTGGATCCGTGATTTACGCCTGAATTCGCTAGGACGGTCGTCGGCAGCAGCGCTCCCACAGTCGCCCGCTCT
CGCCTCGAGAAGCAAGCAAGCCGTCATTGCACTGAACCTGCGCGTAGGTTCGGCAAAGGATGTGGCCGACACTGTCGGTGTATCGCGACCAACGTTGTAT
AACTGGCAGCATCGATTACTTGGCAAAGTGCCCCTAAAACCCATGACAAAGAAGAAAGGTGACACCTCGCTCGAGCAGCGGCATGAGGCACTACTCAGGG
AACTGGCCGAACTGGAGAGCCAGAACCAGCGGCTTCGCATGGAGAATGCAATTCTGGAGAAGGCGAGTGAATTGATAAAAAAGACATGGGCATCAACCCC
CTCGAACTGACAAGCCGAGAAAAAACGAAGGTGGTTGATGCCCTCAGAGTCACGTTTCCATTAGCCAATCTGTTGTGCGGCCTGAAGCTGGCGCGCAGCA
CATACTTCTATCAACGCCTGCGGCAGACGCGGCCCGACAAGTACACGCAGGTGCGTGAGGTCATTCGGACTATCTTCGAGGACAACTACCGCTGCTATGG
CTATCGACGCATTGATAGTGCCTTGCGCCTTGGTGGCATGCGTGTGTCCGAGAAGGTCGTGCGTCGCTTGATGGCGCAAGAGCGTCTGGTCGTGAGAACA
CCGCGCCGCCGGCGCTTCTCGGCGTATGCTGGCGACCCGACACCAGCGGTCCCGAATCTGCTGAATCGCGACTTTCACGCGTCGGCGCCGAATACGAAAT
GGTTGACCGATCTGACGGAAATACACATTCCGGCAGGGAAGGTCTACGTCTCGCCGATCGTCGATTGCTTCGATGGGCTGGTGGTGGCCTGGAATATCGG
CACCAGCCCGGATGCGAACCTGGTCAATACCATGCTGGATCACGCGGTACGGACACTGCGACCCGGTGAGCATCCGGTTATCCATTCGGACAGGGGCTCG
CATTATCGCTGGCCTGCGTGGATCCGCCGCACTGAAAATGCCCAATTAACGCGGTCGATGTCCAAAAAGGGCTGCTCGCCAGACAATGCTGCATGCGAGG
GCTTTTTCGGACGATTGAAGACCGAACTAATCTACCCGAGGAATTGGCAGCACGTGACGCTGAAAGACCTCATGACGCGAATCGATGCCTATATCCACTG
GTACAACGAGCGCCGCATCAAAGTGTCGCTTGGCGGGCGTAGTCCCATCGAGTATCGTCATGCGGTCGGATTGATGTCCGTATAAACCGTCCAAGAAATC
GTCCGCACCCCCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 669 bp | 222 aa | 42 | 710 | + | No |
Description : First part of the transposase
ORF sequence :
MYSYEDRLRAVRLYLKLGRRMSATLRQLGYPTKNSLKAWLAEFERNQDLRRGYQRIKRQYTDEQKQRAVDHYIEQGYCLSHTIRSLGYPSREALRAWIRD
LRLNSLGRSSAAALPQSPALASRSKQAVIALNLRVGSAKDVADTVGVSRPTLYNWQHRLLGKVPLKPMTKKKGDTSLEQRHEALLRELAELESQNQRLRM
ENAILEKASELIKKTWASTPSN
LRLNSLGRSSAAALPQSPALASRSKQAVIALNLRVGSAKDVADTVGVSRPTLYNWQHRLLGKVPLKPMTKKKGDTSLEQRHEALLRELAELESQNQRLRM
ENAILEKASELIKKTWASTPSN
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 912 bp | 304 aa | 671 | 1582 | + | No |
Description : Second part of the transposase
ORF sequence :
IDKKDMGINPLELTSREKTKVVDALRVTFPLANLLCGLKLARSTYFYQRLRQTRPDKYTQVREVIRTIFEDNYRCYGYRRIDSALRLGGMRVSEKVVRRL
MAQERLVVRTPRRRRFSAYAGDPTPAVPNLLNRDFHASAPNTKWLTDLTEIHIPAGKVYVSPIVDCFDGLVVAWNIGTSPDANLVNTMLDHAVRTLRPGE
HPVIHSDRGSHYRWPAWIRRTENAQLTRSMSKKGCSPDNAACEGFFGRLKTELIYPRNWQHVTLKDLMTRIDAYIHWYNERRIKVSLGGRSPIEYRHAVG
LMSV
MAQERLVVRTPRRRRFSAYAGDPTPAVPNLLNRDFHASAPNTKWLTDLTEIHIPAGKVYVSPIVDCFDGLVVAWNIGTSPDANLVNTMLDHAVRTLRPGE
HPVIHSDRGSHYRWPAWIRRTENAQLTRSMSKKGCSPDNAACEGFFGRLKTELIYPRNWQHVTLKDLMTRIDAYIHWYNERRIKVSLGGRSPIEYRHAVG
LMSV
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1541 bp | 514 aa | 42 | 1582 | + | Yes |
Chemistry : DDE
ORF sequence :
MYSYEDRLRAVRLYLKLGRRMSATLRQLGYPTKNSLKAWLAEFERNQDLRRGYQRIKRQYTDEQKQRAVDHYIEQGYCLSHTIRSLGYPSREALRAWIRD
LRLNSLGRSSAAALPQSPALASRSKQAVIALNLRVGSAKDVADTVGVSRPTLYNWQHRLLGKVPLKPMTKKKGDTSLEQRHEALLRELAELESQNQRLRM
ENAILEKASELIKKDMGINPLELTSREKTKVVDALRVTFPLANLLCGLKLARSTYFYQRLRQTRPDKYTQVREVIRTIFEDNYRCYGYRRIDSALRLGGM
RVSEKVVRRLMAQERLVVRTPRRRRFSAYAGDPTPAVPNLLNRDFHASAPNTKWLTDLTEIHIPAGKVYVSPIVDCFDGLVVAWNIGTSPDANLVNTMLD
HAVRTLRPGEHPVIHSDRGSHYRWPAWIRRTENAQLTRSMSKKGCSPDNAACEGFFGRLKTELIYPRNWQHVTLKDLMTRIDAYIHWYNERRIKVSLGGR
SPIEYRHAVGLMSV
LRLNSLGRSSAAALPQSPALASRSKQAVIALNLRVGSAKDVADTVGVSRPTLYNWQHRLLGKVPLKPMTKKKGDTSLEQRHEALLRELAELESQNQRLRM
ENAILEKASELIKKDMGINPLELTSREKTKVVDALRVTFPLANLLCGLKLARSTYFYQRLRQTRPDKYTQVREVIRTIFEDNYRCYGYRRIDSALRLGGM
RVSEKVVRRLMAQERLVVRTPRRRRFSAYAGDPTPAVPNLLNRDFHASAPNTKWLTDLTEIHIPAGKVYVSPIVDCFDGLVVAWNIGTSPDANLVNTMLD
HAVRTLRPGEHPVIHSDRGSHYRWPAWIRRTENAQLTRSMSKKGCSPDNAACEGFFGRLKTELIYPRNWQHVTLKDLMTRIDAYIHWYNERRIKVSLGGR
SPIEYRHAVGLMSV
Blast result :
Comments
IS1353 is inserted inside of of the end of IS1326, the IS21-like element present in the integron In2 (Brown et al., 1996). There might be some problem with the proposed limits of this element: the 2-bp DR and the IR are quite unusual.
References
1] Brown, H.J., Stokes, H.W., and Hall, R.M. (1996) J. Bacteriol. 178, 4429-4437.