IS1203
- Family IS3
- Group IS51
Isoform IS3411Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| U06468 | ND | Escherichia coli | Escherichia coli O111:H- PH |
DNA section
IS Length : 1310 bp
Ends
IR Length : 22/25
IRL : TGAACCGCCCCGGAAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAA
IRR : TGAACCGCCCCGGGTTTCCTGGAGAGTGTTTTATCTGTGAACTCAGGCTG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
TGAACCGCCCCGGAAATCCTGGAGACTAAACTCCCTGAGAAAGAGGTAAACAGGATGACTAAAAATACTCGTTTTTCCCCCGAAGTCCGTCAGCGGGCGA
TTCGTATGGTTCTGGAAAGTCAGGATGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACGCCGGAGACTCTGCGTGT
CTGGGTTCGCCAGCATGAGCGGGATACCGGGGGGAGTGATGGTGGGCTCACCACCGCTGAACGTCAGCGTCTGAAAGAGCTGGAACGTGAAAATCGTGAA
CTGCGCCGCAGTAACGATATCCTTCGCCAGGCTTCCGCTTATTTTCCGAAGGCGGAGTTCGACCGCCTCTGGAAAAAATGATGCCACTGCTGGATAAGCT
GCGTGAGCAGTACGGGGTCGGACCGGTATGCAGCGAACTGCATATTGCCCCGTCAACGTATTACCATTGTCAGCAACAGCGACATCATCCGGATAAACGG
AGTGCCCGTGCGCACCACGACGACTGGCTGAAGAGAGAGATACAGCGCGTATACGATGAAAATCATCAGGTGTACGGTGTGCGTAAAGTCTGGCGTCAGT
TGTTACGGGAAGGAATCAGGGTGGCCAGATGTACAGTGGCACGTCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGAC
CATCAGCCGGAAAGCCGTTGCCGCAGGCGACCGCGTAAACCGTCAGTTCGTGGCAGAACGACCTGACCAGCTGTGGGTGGCTGATTTTACTTACGTCAGC
ACATGGCAGGGCTTCGTCTATGTGGCGTTTATCATTGATGTGTTTGCCGGATACATCGTGGGGTGGCGGGTCTCATCGTCTATGGAAACGACATTCGTGC
TGGATGCGCTGGAGCAGGCGTTGTGGGCCCGTCGTCCGTCTGGCACCATCCATCACAGCGATAAAGGCTCTCAGTATGTGTCACTGGCCTATACGGAGCG
ACTAAAAGAAGCCGGATTACTGGCATCAACAGGGAGTACAGGCGACTCGTATGACAACGCGATGGCTGAGAGCATCAATGGTCTTTACAAAGCGGAGGTA
ATACACCGTAAGAGCTGGAAAAACCGTGCAGAAGTGGAACTGGCCACACTAACGTGGGTGGACTGGTATTACAATCGACGATTGCTGGGAAGGCTGGGCC
ATACTCCTCCGGCAGAAGCAGGAAAAGCTTATTATGCTTCCATCGGAAACGATGATCTGGCAGCCTGAGTTCACAGATAAAACACTCTCCAGGAAACCCG
GGGCGGTTCA
TTCGTATGGTTCTGGAAAGTCAGGATGAATATGACTCACAGTGGGCGGCAATTTGTTCCATTGCCCCAAAGATTGGCTGTACGCCGGAGACTCTGCGTGT
CTGGGTTCGCCAGCATGAGCGGGATACCGGGGGGAGTGATGGTGGGCTCACCACCGCTGAACGTCAGCGTCTGAAAGAGCTGGAACGTGAAAATCGTGAA
CTGCGCCGCAGTAACGATATCCTTCGCCAGGCTTCCGCTTATTTTCCGAAGGCGGAGTTCGACCGCCTCTGGAAAAAATGATGCCACTGCTGGATAAGCT
GCGTGAGCAGTACGGGGTCGGACCGGTATGCAGCGAACTGCATATTGCCCCGTCAACGTATTACCATTGTCAGCAACAGCGACATCATCCGGATAAACGG
AGTGCCCGTGCGCACCACGACGACTGGCTGAAGAGAGAGATACAGCGCGTATACGATGAAAATCATCAGGTGTACGGTGTGCGTAAAGTCTGGCGTCAGT
TGTTACGGGAAGGAATCAGGGTGGCCAGATGTACAGTGGCACGTCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGAC
CATCAGCCGGAAAGCCGTTGCCGCAGGCGACCGCGTAAACCGTCAGTTCGTGGCAGAACGACCTGACCAGCTGTGGGTGGCTGATTTTACTTACGTCAGC
ACATGGCAGGGCTTCGTCTATGTGGCGTTTATCATTGATGTGTTTGCCGGATACATCGTGGGGTGGCGGGTCTCATCGTCTATGGAAACGACATTCGTGC
TGGATGCGCTGGAGCAGGCGTTGTGGGCCCGTCGTCCGTCTGGCACCATCCATCACAGCGATAAAGGCTCTCAGTATGTGTCACTGGCCTATACGGAGCG
ACTAAAAGAAGCCGGATTACTGGCATCAACAGGGAGTACAGGCGACTCGTATGACAACGCGATGGCTGAGAGCATCAATGGTCTTTACAAAGCGGAGGTA
ATACACCGTAAGAGCTGGAAAAACCGTGCAGAAGTGGAACTGGCCACACTAACGTGGGTGGACTGGTATTACAATCGACGATTGCTGGGAAGGCTGGGCC
ATACTCCTCCGGCAGAAGCAGGAAAAGCTTATTATGCTTCCATCGGAAACGATGATCTGGCAGCCTGAGTTCACAGATAAAACACTCTCCAGGAAACCCG
GGGCGGTTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 327 bp | 108 aa | 55 | 381 | + | No |
Description : First part of the transposase
ORF sequence :
MTKNTRFSPEVRQRAIRMVLESQDEYDSQWAAICSIAPKIGCTPETLRVWVRQHERDTGGSDGGLTTAERQRLKELERENRELRRSNDILRQASAYFPKA
EFDRLWKK
EFDRLWKK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 327 bp | 108 aa | 55 | 381 | + | No |
Description : Second part of the transposase
ORF sequence :
RYPSPGFRLFSEGGVRPPLEKMMPLLDKLREQYGVGPVCSELHIAPSTYYHCQQQRHHPDKRSARAHHDDWLKREIQRVYDENHQVYGVRKVWRQLLREG
IRVARCTVARLMAVMGLAGVLRGKKVRTTISRKAVAAGDRVNRQFVAERPDQLWVADFTYVSTWQGFVYVAFIIDVFAGYIVGWRVSSSMETTFVLDALE
QALWARRPSGTIHHSDKGSQYVSLAYTERLKEAGLLASTGSTGDSYDNAMAESINGLYKAEVIHRKSWKNRAEVELATLTWVDWYYNRRLLGRLGHTPPA
EAGKAYYASIGNDDLAA
IRVARCTVARLMAVMGLAGVLRGKKVRTTISRKAVAAGDRVNRQFVAERPDQLWVADFTYVSTWQGFVYVAFIIDVFAGYIVGWRVSSSMETTFVLDALE
QALWARRPSGTIHHSDKGSQYVSLAYTERLKEAGLLASTGSTGDSYDNAMAESINGLYKAEVIHRKSWKNRAEVELATLTWVDWYYNRRLLGRLGHTPPA
EAGKAYYASIGNDDLAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1214 bp | 404 aa | 55 | 1268 | + | Yes |
Chemistry : DDE
ORF sequence :
MTKNTRFSPEVRQRAIRMVLESQDEYDSQWAAICSIAPKIGCTPETLRVWVRQHERDTGGSDGGLTTAERQRLKELERENRELRRSNDILRQASAYFSEG
GVRPPLEKMMPLLDKLREQYGVGPVCSELHIAPSTYYHCQQQRHHPDKRSARAHHDDWLKREIQRVYDENHQVYGVRKVWRQLLREGIRVARCTVARLMA
VMGLAGVLRGKKVRTTISRKAVAAGDRVNRQFVAERPDQLWVADFTYVSTWQGFVYVAFIIDVFAGYIVGWRVSSSMETTFVLDALEQALWARRPSGTIH
HSDKGSQYVSLAYTERLKEAGLLASTGSTGDSYDNAMAESINGLYKAEVIHRKSWKNRAEVELATLTWVDWYYNRRLLGRLGHTPPAEAGKAYYASIGND
DLAA
GVRPPLEKMMPLLDKLREQYGVGPVCSELHIAPSTYYHCQQQRHHPDKRSARAHHDDWLKREIQRVYDENHQVYGVRKVWRQLLREGIRVARCTVARLMA
VMGLAGVLRGKKVRTTISRKAVAAGDRVNRQFVAERPDQLWVADFTYVSTWQGFVYVAFIIDVFAGYIVGWRVSSSMETTFVLDALEQALWARRPSGTIH
HSDKGSQYVSLAYTERLKEAGLLASTGSTGDSYDNAMAESINGLYKAEVIHRKSWKNRAEVELATLTWVDWYYNRRLLGRLGHTPPAEAGKAYYASIGND
DLAA
Blast result :
Comments
IS1203 is located 2 kb upstream of the sltI operon (Shiga-like toxin type I) of Escherichia coli O111:H- strain PH. There are at least 12 IS1203 chromosomal copies in strain PH of Escherichia coli O111:H-. IS1203 ORF2 is 95 and 96 % identical to those of IS3411 and IS629, respectively. Based on comparison with other IS51 group members, the ends of IS1203 (Paton and Paton, 1994) have been reevaluated: 1 bp has been removed at each end. All coordinates have been modified accordingly.
References
1] Paton, A.W., and Paton, J.C. (1994) Gene 150, 67-70.
2] Brunder, W., Schmidt, H., and Karch, H. (1997) Mol. Microbiol. 24, 767-778.
2] Brunder, W., Schmidt, H., and Karch, H. (1997) Mol. Microbiol. 24, 767-778.