IS1165
- Family ISL3
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| X62617 | ND | Leuconostoc mesenteroides | Pediococcus sp. DB1046 Leuconostoc mesenteroides subsp. cremoris DB1165 plasmid p30kb Lactobacillus casei 64H Lactobacillus helveticus CH-1 Lactobacillus lactis Lactobacillus oenos LOP89005 |
DNA section
IS Length : 1553 bp
Ends
IR Length : 20/39
IRL : GGGTCTAGAATTTTTGGTGTTGGAAAGTATTTCCATTCCAAAAGTACTAG
IRR : GGAAGTTTGTCAAATGGTGTTGAAGGATAGTTTCTATCCTTTGGAGATCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| NNNNATTAAA | AATAATAG | TTGGTTNNNN | 8 |
DNA sequence
GGGTCTAGAATTTTTGGTGTTGGAAAGTATTTCCATTCCAAAAGTACTAGGCCCTTTTTGATAACAAAAAAAGGCATCTAAGCCTCCCTAGTGCTATGCT
TTAAGCGACGAAACTCAAGATAAGCGGGGTAGGAATAGATGTCTCAACTAGATAATACACTTAAATTACTGGGAATTACAGACACAAACATTCAGGTGTT
CGGTACTCGTGATGAATTTCATGGTCGGGGCTCGGGTCGCAAAAAGTATTTGGTCATCCAGGCAGAGCTTACCTACACACTCAGGCGCTGTCCCAGCTGT
GGATACAATACGTTGCACCCTAACGGACACAAGCTCACTCATGTCCACATTGCAGGACCCATGGACCGGCCCGTAATTCTAGAGCTAAACAAGCAACGCT
GGCGTTGTAGTAACTGCCATAGCACTTGTACGGCCACCACTCCAGTGGTATCAACCAACCACGCCATCGGTCACGGACTAGCGACTCATGTGCTGAAGCT
AGCCAGTAAATCACTCCCGGCTAAGACTATCGCCAGTCTCACCGGTATTTCGACAAACTCAGTTCAGCGTATTTTGACGGCTAATATTCATCCACACGCG
AGCCGCCGGTTACCGATTAATCTATGCTTTGATGAATTCCGTTCCACGCACGGCTCCATGTCGTTTATTTGCATTGACGCCGACACTCACAAATCAGTTA
AAGTACTTAGCGACCGCCTTAATAGAACCATCAAACAGTTCTTTCTTAGTCAGTACAGCACCGCAGAATGGGCCGCGGTTCAACGCGTCATCATGGACAT
GAACGCCTCCTATCAGGCATTCGTGCACGAACTATTCCCTAACGCCGAACTCATTATTGATCGGTTCCACATTATTCAATTAATGGGCCGGACGATGGAT
ACCATTCGCACTCAATGTTTAAAGCAACTCGACAAGCATTCGCGGGAATATAAAGTACTGAAATCACTATGGCGCCTATTACACAAGGCCAACCCTGACG
CACAAAAGAGCCGCTACCTCTTCGGCCTGAATGAGTACTCGACCGAACAGAACGCTATTGATATTGGAACCGATACGTTTCCGGCCTTCAAAACAGCTTA
TGAAACCTACATCGATCTCCACGATGCTTTGATGGGGCGTCACGCTGATGAACTCAAGAATATCATCACTAACTATCAACCTAACGGCACGCCCCTAGAT
ACGGCCATGCATACCCTACGAAAGAATCTTAATGGAGTAATTAACGCCGCCAAATCGTCCTACTCTAACGGACCGATAGAGGGCATCAACCGTAAGATCA
AGGAGCTCAAACGTGCTTGTTATGGCTTCTCCAATCAGGCCAATATGTTCACACGCATCTACCAGCTGATTGCCTAGATTATCTACCACAATAACGTCAC
GATGGTAGGCTTATTTGTTATGCCTTCAAGTACGATATTCAGACAACACACCAGATTAAACGCCGCAACTAATAAAACAACAAAAAAGATCTCCCACACG
AGGAGATCTCCAAAGGATAGAAACTATCCTTCAACACCATTTGACAAACTTCC
TTAAGCGACGAAACTCAAGATAAGCGGGGTAGGAATAGATGTCTCAACTAGATAATACACTTAAATTACTGGGAATTACAGACACAAACATTCAGGTGTT
CGGTACTCGTGATGAATTTCATGGTCGGGGCTCGGGTCGCAAAAAGTATTTGGTCATCCAGGCAGAGCTTACCTACACACTCAGGCGCTGTCCCAGCTGT
GGATACAATACGTTGCACCCTAACGGACACAAGCTCACTCATGTCCACATTGCAGGACCCATGGACCGGCCCGTAATTCTAGAGCTAAACAAGCAACGCT
GGCGTTGTAGTAACTGCCATAGCACTTGTACGGCCACCACTCCAGTGGTATCAACCAACCACGCCATCGGTCACGGACTAGCGACTCATGTGCTGAAGCT
AGCCAGTAAATCACTCCCGGCTAAGACTATCGCCAGTCTCACCGGTATTTCGACAAACTCAGTTCAGCGTATTTTGACGGCTAATATTCATCCACACGCG
AGCCGCCGGTTACCGATTAATCTATGCTTTGATGAATTCCGTTCCACGCACGGCTCCATGTCGTTTATTTGCATTGACGCCGACACTCACAAATCAGTTA
AAGTACTTAGCGACCGCCTTAATAGAACCATCAAACAGTTCTTTCTTAGTCAGTACAGCACCGCAGAATGGGCCGCGGTTCAACGCGTCATCATGGACAT
GAACGCCTCCTATCAGGCATTCGTGCACGAACTATTCCCTAACGCCGAACTCATTATTGATCGGTTCCACATTATTCAATTAATGGGCCGGACGATGGAT
ACCATTCGCACTCAATGTTTAAAGCAACTCGACAAGCATTCGCGGGAATATAAAGTACTGAAATCACTATGGCGCCTATTACACAAGGCCAACCCTGACG
CACAAAAGAGCCGCTACCTCTTCGGCCTGAATGAGTACTCGACCGAACAGAACGCTATTGATATTGGAACCGATACGTTTCCGGCCTTCAAAACAGCTTA
TGAAACCTACATCGATCTCCACGATGCTTTGATGGGGCGTCACGCTGATGAACTCAAGAATATCATCACTAACTATCAACCTAACGGCACGCCCCTAGAT
ACGGCCATGCATACCCTACGAAAGAATCTTAATGGAGTAATTAACGCCGCCAAATCGTCCTACTCTAACGGACCGATAGAGGGCATCAACCGTAAGATCA
AGGAGCTCAAACGTGCTTGTTATGGCTTCTCCAATCAGGCCAATATGTTCACACGCATCTACCAGCTGATTGCCTAGATTATCTACCACAATAACGTCAC
GATGGTAGGCTTATTTGTTATGCCTTCAAGTACGATATTCAGACAACACACCAGATTAAACGCCGCAACTAATAAAACAACAAAAAAGATCTCCCACACG
AGGAGATCTCCAAAGGATAGAAACTATCCTTCAACACCATTTGACAAACTTCC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1239 bp | 412 aa | 139 | 1377 | + | No |
Chemistry : Unknow
ORF sequence :
MSQLDNTLKLLGITDTNIQVFGTRDEFHGRGSGRKKYLVIQAELTYTLRRCPSCGYNTLHPNGHKLTHVHIAGPMDRPVILELNKQRWRCSNCHSTCTAT
TPVVSTNHAIGHGLATHVLKLASKSLPAKTIASLTGISTNSVQRILTANIHPHASRRLPINLCFDEFRSTHGSMSFICIDADTHKSVKVLSDRLNRTIKQ
FFLSQYSTAEWAAVQRVIMDMNASYQAFVHELFPNAELIIDRFHIIQLMGRTMDTIRTQCLKQLDKHSREYKVLKSLWRLLHKANPDAQKSRYLFGLNEY
STEQNAIDIGTDTFPAFKTAYETYIDLHDALMGRHADELKNIITNYQPNGTPLDTAMHTLRKNLNGVINAAKSSYSNGPIEGINRKIKELKRACYGFSNQ
ANMFTRIYQLIA
TPVVSTNHAIGHGLATHVLKLASKSLPAKTIASLTGISTNSVQRILTANIHPHASRRLPINLCFDEFRSTHGSMSFICIDADTHKSVKVLSDRLNRTIKQ
FFLSQYSTAEWAAVQRVIMDMNASYQAFVHELFPNAELIIDRFHIIQLMGRTMDTIRTQCLKQLDKHSREYKVLKSLWRLLHKANPDAQKSRYLFGLNEY
STEQNAIDIGTDTFPAFKTAYETYIDLHDALMGRHADELKNIITNYQPNGTPLDTAMHTLRKNLNGVINAAKSSYSNGPIEGINRKIKELKRACYGFSNQ
ANMFTRIYQLIA
Blast result :
Comments
IS1165 copy number varies from 4 to 13 in Leuconostoc mesenteroides subsp. cremoris, allowing genetic fingerprints of this bacteria. 154 bp downstream of the FIIIlac gene from Lactobacillus casei (Alpert and Chassy, 1988) lies a sequence of 41 bp nearly identical to the right end of IS1165.
References
1] Alpert, C.-A., and Chassy, B.M. (1988) Gene 62, 277-288.
2] Johansen, E., and Kibemich, A. (1992) Plasmid 27, 200-206.
2] Johansen, E., and Kibemich, A. (1992) Plasmid 27, 200-206.