ISfinder Mirror Site

ISAzo25

Family IS1182
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_006513	ND	Azoarcus sp.	Aromatoleum aromaticum EbN1 Azoarcus sp. EbN1

DNA section

IS Length : 1388 bp

Ends

IR Length : 9/11

IRL : GAGTTCTGGTAGCGGCAGCCTGTTATTGACACTCGAGCCTCTTGCAAAAG
IRR : GAGTCCTGCGTCATCGACGGCAAGGGTGTCAGGGCAGGCGTTGAGGTGCG

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CTCCGGCGCG		GCTCCGTCCA	0

DNA sequence

GAGTTCTGGTAGCGGCAGCCTGTTATTGACACTCGAGCCTCTTGCAAAAGGAGCTCTGCACGGGGGATTTTTTGCGGCGGGAGGCAAAGGCGGAGGGTGC
GGGCGGCCATTTCTGGCATGATGCGGTTATCGAAGCCAACATCATAACGCCCGCCCCATGGATGCTATCCGACGCGAACTGATCCTGCAACGCTGGAACG
TGATTCAGCACGAACTGCTGCCCGAGTTGAAGAACGAGGTGGGCGCGCTGACGCCGAAGCTGGAGAAAGTCATCCACACCCTGGAATGGGTGCGCATCGA
GGAATTCACGGCGTCGACATGGTGTCGGGTGGGGCGTCCGCCCTGTGAGCGGGCCTGGCTGGCGAACGCTTTCGTCGCGAAGGCGGTGCTGGGACTGACG
ACCACGGTGGGGCTGATCGAGCGCCTGACGGTGGATCGTGCGCTGCGGCGCATCTGTGGTTTCCCGCTGTGCCGCAAGCTGCCCTCCGAAGCCACGTTCT
CGCGCGCGTTCGACGAGTTTGCCGCGGCGCGTCTGGCCGAACGGGTGCATGAGGCGCTCATCAAGGAACACCTTGGGGATGAACTGATCGGGCACCTCAG
CCGCGATGGCACCGCGATCGAAGCGCGCGAGCGCCCGGCCCACACCAGGGGTGCGGCGGCGACACCGGCGCCTGCGGCCCAATCGTCCCTGAGCATCGAG
GAAGAAACCGCCGCGCCAACGCCACCGGTGCCGGCGAAGAAACGCGGCCGCGGCCGCCCCCGGCGCGGCGAAGTCCGTGCCCCCGCCAAGGTTTCGCCCA
TCCAGCGCCAACGCCAGCAGCGCCTAGCGCAGAGGCTCGAGGACATCCCCACGGTGTGCGACCGCGGCACCAAGTGCAATGCCCAGGGGTACAAAGTCAG
CTGGAACGGCTACAAGCTGCACCTGGACACCGCCGACTGTGGCGTGCCGATTGCGGCGCTGCTGTCGTCGGCCTCGATGCACGACAGCCGCGCCGCCATC
CCCCTGTCGCTGATCAGCGCTCAGCGCGTCACCAACCTCTACGACGTCATGGATGCCGCCTACTGCAGCTTCGCGTTGCATGAGCACTGCCGCAGCCTCG
GCCATGTCCCCTTGATCGACCACAATCCGCGCGGCGGCATGAAGGAGGAGTTCGAGCCTGCCGACGCGATCCGTTACAACGAACGCACCGTCGCCGAGCG
CAGCAATGCACGGCTCAAGGATGAGTTCGGCGGCAACACCGTCCGCGTCAAGGGCGGCACCAAGGTCATGGGACACCTGATGTTCGGTGTCCTGGCGCTG
TCTGCCGATCAGTTGATGCGATTGCGACAATGACACGCCGCACCTCAACGCCTGCCCTGACACCCTTGCCGTCGATGACGCAGGACTC

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1095 bp	364 aa	239	1333	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MGALTPKLEKVIHTLEWVRIEEFTASTWCRVGRPPCERAWLANAFVAKAVLGLTTTVGLIERLTVDRALRRICGFPLCRKLPSEATFSRAFDEFAAARLA
ERVHEALIKEHLGDELIGHLSRDGTAIEARERPAHTRGAAATPAPAAQSSLSIEEETAAPTPPVPAKKRGRGRPRRGEVRAPAKVSPIQRQRQQRLAQRL
EDIPTVCDRGTKCNAQGYKVSWNGYKLHLDTADCGVPIAALLSSASMHDSRAAIPLSLISAQRVTNLYDVMDAAYCSFALHEHCRSLGHVPLIDHNPRGG
MKEEFEPADAIRYNERTVAERSNARLKDEFGGNTVRVKGGTKVMGHLMFGVLALSADQLMRLRQ

Blast result :

Comments

ISAzo25 is 44% aa similar to IS656.
Position of the ends of ISAzo25 could not be precisely determined.

References

1] Kuhner,S., Wohlbrand,L., Fritz,I., Wruck,W., Hultschig,C., Hufnagel,P., Kube,M., Reinhardt,R. and Rabus,R. (2005) J. Bacteriol. 187 (4), 1493-1503.

2] Rabus,R., Kube,M., Heider,J., Beck,A., Heitmann,K., Widdel,F. and Reinhardt,R. (2005) Arch. Microbiol. 183 (1), 27-36.

3] PROSCIENCE (2004) Direct Submission GenBank.

4] ISfinder annotation (2008).