ISEnfa3
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AF469486 | ND | Enterococcus faecium | Enterococcus faecium |
DNA section
IS Length : 1416 bp
Ends
IR Length : 21/25
IRL : TGATGTGCACCCCAAAAGTTAGACTAGAAATCTAATTAAAAGGGGTGCTT
IRR : TGAACTGAACCCCAAAAGTTGAACTATTTAATGGACTGTTTCCGATATTC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
TGATGTGCACCCCAAAAGTTAGACTAGAAATCTAATTAAAAGGGGTGCTTTTTTTGCGGAAATATACATTTGTTTTTAAGAAAAAAGTAGTTTCAGACTA
TTTAAATAACGAAGGCGGCTACAAATATCTTGCACATAAATATCAAATAAATCGTACACTGGTTAGACATTGGGTAAGGATTTATAACTATCATGGTTGG
GAAGGCTTGGTTGGAGGTGGCAAAAGCTACACTACAAAATTTAAACTTGATGTTATAGAATATATGGAAACAAATGGTCTTTCTATCCAAGAAACTGCTA
AAAAATTTAATATCGGTTCAAATAGAACTCTAAGTAAATGGATAGAGCAATATGAAGAAGGCGGTGCTTCTTCACTTGAGAGCCAAAAAAGGGGCAGAAA
AATTAGTATGAATTCCAAGCTAAACATTCCTAAAAAACTTAAAGATGAGTCTCTTGAAGAAGAAGTTATTCGTTTAAGAGCAGAGAACGCATATTTAAAA
AAGTTAGAAACCTTGATTCAAGAACAAGATTTGAGCAAGAAGAAATCAAGGTTAAAACAATCCTTCAGCTCAAAAAAGAATTTAAATTGAATCTATTACT
TTCAATTGCACAATTAGCGAAGTCAACTTATTATTACTGGGTAAAAAAATTAGATAAGCCAGATAAATATAGCAAGATAAAGCAAGAAATTACAGCGATT
GTAAAAGAATCAAGAAACTCTTATGGTTATCGTAGAGTCACTTTAGCGTTAAAGATGAAAGGATATACAATCAATCATAAAACAGTTAGAAAATTAATGT
CCCAAATGGGACTTACCTGTCAAATCCGAATAAAGCGATATAAATCTTATAAAGGAACAGTAGGAAAAATTGCCAAGAATGTGTTAAAACGAAATTTTTC
AGTAGATACACCCAATAAAAAATGGGTGACAGACGTCACTGAATTCAAGATAAAAGGAAGAAAAATCTATCTATCTCCTATACTTGATTTATTTAACGGA
GAAATAATTAGTTATAGTATATCAACTAGTCCAACATATAAACTGATTGAAGAAATGCTTCAACAAGCAATTAAAAAAAAGGGAACTGAGGGTTCACTGA
TTCTACATTCAGATCAAGGATGGCAGTATCAGATGCCACAATATCAAAAAAAATTAAAAGAGAATAACATTATTCAAAGCATGTCTAGAAAAGGAAATTG
CTTAGATAATTCTGTAATAGAAAATTTTTTTGGTGTGCTTAAATCAGAGTTTTTTTATCGAGAAAAATTCCGATCAATTGAGATATTTCAAAGTAAATTA
AATGAGTATATTAGGTGGTATAATAACAAAAGGATAAAACTAAAGTTAAATGGACTATCTCCAGTAGAATATCGGAAACAGTCCATTAAATAGTTCAACT
TTTGGGGTTCAGTTCA
TTTAAATAACGAAGGCGGCTACAAATATCTTGCACATAAATATCAAATAAATCGTACACTGGTTAGACATTGGGTAAGGATTTATAACTATCATGGTTGG
GAAGGCTTGGTTGGAGGTGGCAAAAGCTACACTACAAAATTTAAACTTGATGTTATAGAATATATGGAAACAAATGGTCTTTCTATCCAAGAAACTGCTA
AAAAATTTAATATCGGTTCAAATAGAACTCTAAGTAAATGGATAGAGCAATATGAAGAAGGCGGTGCTTCTTCACTTGAGAGCCAAAAAAGGGGCAGAAA
AATTAGTATGAATTCCAAGCTAAACATTCCTAAAAAACTTAAAGATGAGTCTCTTGAAGAAGAAGTTATTCGTTTAAGAGCAGAGAACGCATATTTAAAA
AAGTTAGAAACCTTGATTCAAGAACAAGATTTGAGCAAGAAGAAATCAAGGTTAAAACAATCCTTCAGCTCAAAAAAGAATTTAAATTGAATCTATTACT
TTCAATTGCACAATTAGCGAAGTCAACTTATTATTACTGGGTAAAAAAATTAGATAAGCCAGATAAATATAGCAAGATAAAGCAAGAAATTACAGCGATT
GTAAAAGAATCAAGAAACTCTTATGGTTATCGTAGAGTCACTTTAGCGTTAAAGATGAAAGGATATACAATCAATCATAAAACAGTTAGAAAATTAATGT
CCCAAATGGGACTTACCTGTCAAATCCGAATAAAGCGATATAAATCTTATAAAGGAACAGTAGGAAAAATTGCCAAGAATGTGTTAAAACGAAATTTTTC
AGTAGATACACCCAATAAAAAATGGGTGACAGACGTCACTGAATTCAAGATAAAAGGAAGAAAAATCTATCTATCTCCTATACTTGATTTATTTAACGGA
GAAATAATTAGTTATAGTATATCAACTAGTCCAACATATAAACTGATTGAAGAAATGCTTCAACAAGCAATTAAAAAAAAGGGAACTGAGGGTTCACTGA
TTCTACATTCAGATCAAGGATGGCAGTATCAGATGCCACAATATCAAAAAAAATTAAAAGAGAATAACATTATTCAAAGCATGTCTAGAAAAGGAAATTG
CTTAGATAATTCTGTAATAGAAAATTTTTTTGGTGTGCTTAAATCAGAGTTTTTTTATCGAGAAAAATTCCGATCAATTGAGATATTTCAAAGTAAATTA
AATGAGTATATTAGGTGGTATAATAACAAAAGGATAAAACTAAAGTTAAATGGACTATCTCCAGTAGAATATCGGAAACAGTCCATTAAATAGTTCAACT
TTTGGGGTTCAGTTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 537 bp | 178 aa | 54 | 590 | + | No |
Description : First part of the transposase
ORF sequence :
LRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIYNYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQY
EEGGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYLKKLETLIQEQDLSKKKSRLKQSFSSKKNLN
EEGGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYLKKLETLIQEQDLSKKKSRLKQSFSSKKNLN
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 936 bp | 311 aa | 458 | 1393 | + | No |
Description : Second part of the transposase
ORF sequence :
RRSYSFKSRERIFKKVRNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKM
KGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEEM
LQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGL
SPVEYRKQSIK
KGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEEM
LQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGL
SPVEYRKQSIK
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1340 bp | 446 aa | 54 | 1393 | + | Yes |
Chemistry : DDE
ORF sequence :
LRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIYNYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQY
EEGGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYLKKVRNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKL
DKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKI
KGRKIYLSPILDLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENFFGVLKSEF
FYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK
EEGGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYLKKVRNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKL
DKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKI
KGRKIYLSPILDLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENFFGVLKSEF
FYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK
Blast result :
Comments
ISEnfa3 is 56% aa similar to ISKpn1 (ORF1) and 76% aa to ISPsy9 (ORF2).
There is a frameshift at position 502. So the ORF3 is the ORFAB, a fusion ORF.
There is a frameshift at position 502. So the ORF3 is the ORFAB, a fusion ORF.
References
1] Lee,W.G. and Kim,W. (2003) Lett. Appl. Microbiol. 36 (3), 186-190