ISLjo5

  • Family IS200/IS605
  • Group IS605
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_005362 ND Lactobacillus johnsonii
Lactobacillus johnsonii NCC 533
DNA section
IS Length : 2101 bp

Ends


Left end : TGAAATCCGCCCGTAAATCCCCGTATTTTAATGCGGGGATGGATAGGGCTTACCGTCTTTGATTGCGTTATAGACTTGATCGTTGATGTATTTCTCTACC II struct. : Yes
Right end : CAATTCAAACAAGTTAAGCCTGTATCTAAGCAAATTGGTGAAATAGTTAGCGCAAGCTAACGAACCACCAAGCCCCAGAATTCATTCTAGGGTAGTTGAC II struct. : Yes

Insertion site


Left flankLE cleavage siteRight flankRE cleavage site
TCTAAAAATCACTTTTTTAACACTATAAACtgac

DNA sequence

TGAAATCCGCCCGTAAATCCCCGTATTTTAATGCGGGGATGGATAGGGCTTACCGTCTTTGATTGCGTTATAGACTTGATCGTTGATGTATTTCTCTACC
ACTTCTTTGGACATATTGCCTACACTGCCAAAATAATAGCTTGGCGACCATAGATGTCCGCCCCACATCTTGTTTTGCCTAATTTCGGGATGTGCCTGCA
AAAACAACCTGCCACTTCTACCTTTTAGAGCCTTGATTACACTAGAACCAGATTTAGAGGGCTTAAAGCTAACCAATAAGTGAACATGTTCGGGCATGAT
CTCCATTTTTTCTATCTTAATTTGATTATCCTCGGCAATCTGCAATAAAAGCTTTCGCATTTCTTCGGCTAAATTGGTATTTGTAAAAATTTGATTGCGG
TATTTGGTACACCAAATCAAATGATAATGAGTATTGTAGACGTAGTGCTTTTCATAGCCTGCGTCTTTTATTTTGTCTTTCTTTTTTATCATAGAGAATA
TTGCTAACCTCTTTGTTTATAAGTGTTTACTATTGATTATAATATCTACATAGTCTATAATCAAATCATATCAATAAAAGGGGGTGAAAAAATGAAAAGA
ATGAGCAGTTTAGAATATCACTTTGGCGTAAAAGTAAGATTCTATCCTAGCAATAATCAAAAGAAAATGATTAAGCAGAATTATGACGCACAGCGTTTTG
TTTATAACCAATACGTTGGCGCTGGCCGTTTGATCTATCACGTTAAAAAGGCCAATAGGATCAAACAGCTTAGCAGCGGTTTGCCCTTTGTAATGCAGGG
AATGTCTGAATATGAAAATCAACAGGCATTAAGAATGATTGATACGCAAGACTTAATTTCCAAACCTAAAAATATTAGAGATAAATATGATTTCTTGCGT
GTTGAAGACATTGATAGCTTGGCTATCGCCAATGCAATTCAAAATTATCACAAAGCATGGAGAAACTATCATAAAATTGGCTATGGAATACCAACCTTTC
ACAAGAAACGAAGCAGCTGGTCATATCAGACTAACTGTCAGTACATAAAGCAAGACGCAGCCTATCTTGATAATGGTACGGTTAGATTTATTGACGCTAA
GCATGTTAAGCTTCCTAAACTGGGAATAGTGAGAATTGCTGGATTGCGCAAGCTGATTAAAAAGCGTTTGTTTAACCGTATTCCTATTCGAATCGGTACT
GCCTCAATTAAGAAAACGGCAGACGATCAGTTTTATCTTTCTTTACAGCTGGGAAGTGATATTGCCTTTGTCAAAGACTATGCTAAAACACAAAAGCAGA
TCGGCATTGATCTTAACTTGGATAATTTCTTGACGGATTCTAACGGTGCAATGGTAGCTAATCCAAGGTTTTATCGCAGAGCCAAGAAAAAGCTCGCCCA
CGCTCAAAGAATACTTTCACGTAGAGAACGCAGAGCCAAAAAAGAAGGACGCTCTTTGTGTGAATCCAAGAACTATCAAAAACAGCGCCTAGCCGTAGCT
AAACTGCACGACAGGATTCGCAGACAGAGACAAGACTTCTTGCAGGTTCTTTCTACTGCATTAATCAAAAACCACGATTTAGTAGTAGCCGAAGAACTAA
GAAGTAAAAATCTGTTAAAGAATCATGCTCTTGCCCAATCTATTTCTGATGCAGGATGGCGGAGTTTCTTAACCATGCTCGAATATAAAGCTGATCTTCA
TGGCAAAAAGTTTGTCACGATTGACCCAAAATTTACTACGCAAAGGTGTCATAATTGCGGAAACATAATGGGACAAAACGGCTATAAGAAACTTACGCTT
AAGGATCGAAAATGGACTTGCCCAGTATGCGGAAGTTATCACATAAGAGATTGGAATGCTTCTGTAAACATCTTAGAAAAAAGCAAAGGTATTTGGAAAA
ATCCTAAAATAAAAAAGCGATCTAAATAGACTGCAGTCCGTTTGGCAACTTGCAGACTCAAAAGGCTTTGGTAATTGGGAAAACCGCTGTTTGGATTTTC
GCAATTCAAACAAGTTAAGCCTGTATCTAAGCAAATTGGTGAAATAGTTAGCGCAAGCTAACGAACCACCAAGCCCCAGAATTCATTCTAGGGTAGTTGA
C
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
465 bp154 aa49228-No
ORF function : Transposase
Chemistry : Y1

ORF sequence :

MIKKKDKIKDAGYEKHYVYNTHYHLIWCTKYRNQIFTNTNLAEEMRKLLLQIAEDNQIKIEKMEIMPEHVHLLVSFKPSKSGSSVIKALKGRSGRLFLQA
HPEIRQNKMWGGHLWSPSYYFGSVGNMSKEVVEKYINDQVYNAIKDGKPYPSPH

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1338 bp445 aa5921929+No
ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MKRMSSLEYHFGVKVRFYPSNNQKKMIKQNYDAQRFVYNQYVGAGRLIYHVKKANRIKQLSSGLPFVMQGMSEYENQQALRMIDTQDLISKPKNIRDKYD
FLRVEDIDSLAIANAIQNYHKAWRNYHKIGYGIPTFHKKRSSWSYQTNCQYIKQDAAYLDNGTVRFIDAKHVKLPKLGIVRIAGLRKLIKKRLFNRIPIR
IGTASIKKTADDQFYLSLQLGSDIAFVKDYAKTQKQIGIDLNLDNFLTDSNGAMVANPRFYRRAKKKLAHAQRILSRRERRAKKEGRSLCESKNYQKQRL
AVAKLHDRIRRQRQDFLQVLSTALIKNHDLVVAEELRSKNLLKNHALAQSISDAGWRSFLTMLEYKADLHGKKFVTIDPKFTTQRCHNCGNIMGQNGYKK
LTLKDRKWTCPVCGSYHIRDWNASVNILEKSKGIWKNPKIKKRSK

 

Blast result :
Comments
ISLjo5 is 65% aa similar to ISDra2 (ORF1) and 47% to IS1341 (ORF2).
ORFA is on the complementary strand.
References
1] Pridmore,R.D., Berger,B., Desiere,F., Vilanova,D., Barretto,C., Pittet,A.C., Zwahlen,M.C., Rouvet,M., Altermann,E., Barrangou,R., Mollet,B., Mercenier,A., Klaenhammer,T., Arigoni,F. and Schell,M.A. (2004) Proc. Natl. Acad. Sci. U.S.A. 101 (8), 2512-2517