ISLpl3
- Family IS5
- Group IS427
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_004567 | ND | Lactobacillus plantarum | Lactobacillus plantarum WCFS1 |
DNA section
IS Length : 852 bp
Ends
IR Length : 16/18
IRL : GGGACTGTCTGAAAACTAAAAATTCAGGTATAATCTGAGGAAAAACGAAT
IRR : GGGTCCGTCTGAAAACTACTTAAGTAAGATGCAAATTGAGGCAATGTAGA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CGCTTTCTAC | TA | GTATAAATTA | 2 |
DNA sequence
GGGACTGTCTGAAAACTAAAAATTCAGGTATAATCTGAGGAAAAACGAATCGAGGCATTCCGATGACAACACCTAAACGATACGAACTGGAAGATGCTCA
GTGGGACCGAATCAAAGGATACTTCCCGCCATACCGGACTGGCCGTCCATCAAGCCTAGACAACCGTACCGCCCTCAACGCTATCCTCTGGCTCATGCGC
AGCGGGGCTCCTTGGCGTGATCTACCTGAACGCTATGGCTCTTGGAAAACGGTGTATAGTCGCTTCCGAGCCTGGGTAAGTTCAGGCTTGTTCGAACAGG
TTTTTCTCGAATTGATTGACGATCCCGACATGGAAAACTTGAGCTTAGATTCAACGATCGTTCGAGCGCATCAAAAGGCCACTGGGGCAAAAAAACGCCG
AATGTATGGTCGAAAATCAAGCTATTGGATTAAGTCGAGGTGGCCGAACGACCAAGATTCACGCACTCGTTGACGGATTAGGGAATCCCTTGGGTTTCCG
CCTAACAGGTGGTCAAGTACATGATAGCCAAGTTGCCAGTGAGTTACTGGAAGGCTTCGATATTTCTCAATCAAATATTATCGCGGATAAAGCCTATGGC
ACCGCGAAACTTCGCCAGTATATTGAAGATAAAGCAGGCGTCTATACCATTCCGCCAAAGGAAAATACCAAAGACAAGTGGACCTGTGATTACCACGTTT
ATTGTGAGCGCCATTTGATTGAGAACTTCTTCAATCAGTTGAAGAACTTTCGTAGGATTGCAACGCGTTATGATAAGCTCGCTCATGTTTATCTGGCTAC
GGTCTACATTGCCTCAATTTGCATCTTACTTAAGTAGTTTTCAGACGGACCC
GTGGGACCGAATCAAAGGATACTTCCCGCCATACCGGACTGGCCGTCCATCAAGCCTAGACAACCGTACCGCCCTCAACGCTATCCTCTGGCTCATGCGC
AGCGGGGCTCCTTGGCGTGATCTACCTGAACGCTATGGCTCTTGGAAAACGGTGTATAGTCGCTTCCGAGCCTGGGTAAGTTCAGGCTTGTTCGAACAGG
TTTTTCTCGAATTGATTGACGATCCCGACATGGAAAACTTGAGCTTAGATTCAACGATCGTTCGAGCGCATCAAAAGGCCACTGGGGCAAAAAAACGCCG
AATGTATGGTCGAAAATCAAGCTATTGGATTAAGTCGAGGTGGCCGAACGACCAAGATTCACGCACTCGTTGACGGATTAGGGAATCCCTTGGGTTTCCG
CCTAACAGGTGGTCAAGTACATGATAGCCAAGTTGCCAGTGAGTTACTGGAAGGCTTCGATATTTCTCAATCAAATATTATCGCGGATAAAGCCTATGGC
ACCGCGAAACTTCGCCAGTATATTGAAGATAAAGCAGGCGTCTATACCATTCCGCCAAAGGAAAATACCAAAGACAAGTGGACCTGTGATTACCACGTTT
ATTGTGAGCGCCATTTGATTGAGAACTTCTTCAATCAGTTGAAGAACTTTCGTAGGATTGCAACGCGTTATGATAAGCTCGCTCATGTTTATCTGGCTAC
GGTCTACATTGCCTCAATTTGCATCTTACTTAAGTAGTTTTCAGACGGACCC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 411 bp | 136 aa | 63 | 473 | + | No |
Description : First part of the transposase
ORF sequence :
MTTPKRYELEDAQWDRIKGYFPPYRTGRPSSLDNRTALNAILWLMRSGAPWRDLPERYGSWKTVYSRFRAWVSSGLFEQVFLELIDDPDMENLSLDSTIV
RAHQKATGAKKRRMYGRKSSYWIKSRWPNDQDSRTR
RAHQKATGAKKRRMYGRKSSYWIKSRWPNDQDSRTR
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 489 bp | 162 aa | 349 | 837 | + | No |
Description : Second part of the transposase
ORF sequence :
IQRSFERIKRPLGQKNAECMVENQAIGLSRGGRTTKIHALVDGLGNPLGFRLTGGQVHDSQVASELLEGFDISQSNIIADKAYGTAKLRQYIEDKAGVYT
IPPKENTKDKWTCDYHVYCERHLIENFFNQLKNFRRIATRYDKLAHVYLATVYIASICILLK
IPPKENTKDKWTCDYHVYCERHLIENFFNQLKNFRRIATRYDKLAHVYLATVYIASICILLK
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 775 bp | 258 aa | 63 | 837 | + | Yes |
Chemistry : DDE
ORF sequence :
MTTPKRYELEDAQWDRIKGYFPPYRTGRPSSLDNRTALNAILWLMRSGAPWRDLPERYGSWKTVYSRFRAWVSSGLFEQVFLELIDDPDMENLSLDSTIV
RAHQKATGAKKNAECMVENQAIGLSRGGRTTKIHALVDGLGNPLGFRLTGGQVHDSQVASELLEGFDISQSNIIADKAYGTAKLRQYIEDKAGVYTIPPK
ENTKDKWTCDYHVYCERHLIENFFNQLKNFRRIATRYDKLAHVYLATVYIASICILLK
RAHQKATGAKKNAECMVENQAIGLSRGGRTTKIHALVDGLGNPLGFRLTGGQVHDSQVASELLEGFDISQSNIIADKAYGTAKLRQYIEDKAGVYTIPPK
ENTKDKWTCDYHVYCERHLIENFFNQLKNFRRIATRYDKLAHVYLATVYIASICILLK
Blast result :
Comments
ISLpl3 is 63% aa similar to IS298 (ORF1) and 64% to ISCc1 (ORF2).
There is only one copy of ISLpl3 in Lactobacillus plantarum genome.
There is only one copy of ISLpl3 in Lactobacillus plantarum genome.
References
1] Kleerebezem,M., Boekhorst,J., van Kranenburg,R., Molenaar,D., Kuipers,O.P., Leer,R., Tarchini,R., Peters,S.A., Sandbrink,H.M., Fiers,M.W., Stiekema,W., Lankhorst,R.M., Bron,P.A., Hoffer,S.M., Groot,M.N., Kerkhoven,R., de Vries,M., Ursing,B., de Vos,W.M. and Siezen,R.J. (2004) Proc. Natl. Acad. Sci. U.S.A. 100 (4), 1990-1995