ISfinder Mirror Site

ISSau4

Family IS3
Group IS150

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_002952	ND	Staphylococcus aureus	Staphylococcus aureus subsp. aureus MRSA252 Staphylococcus aureus subsp. aureus Mu50 Staphylococcus epidermidis ATCC 12228 Staphylococcus aureus subsp. aureus N315

DNA section

IS Length : 1261 bp

Ends

IR Length : 27/35

IRL : TGACCCCAATTAGTGGGAATTATATAAAAACACTTTCGTTGAATTCATAT
IRR : TGAACCCCTAAAACGGGAACTTAATAAAAACACCATGTTCTAGGCTATTA

Insertion site

Left flank	Direct repeat	Right flank	DR Length

DNA sequence

TGACCCCAATTAGTGGGAATTATATAAAAACACTTTCGTTGAATTCATATTAAAATGAATCATACGGAGGTGTTTTTTCTATGAAAAGAGTTTCTTATTC
AGTAGAAACAAAGTATAAAGCAGTTGAAATGAAAGCAGCAGGATTTTCAACAAAAGAAATTATGAAAGAATTAAATATTAAAAATAGAACACAAGTGGAA
ACTTGGTGGCGATGGTATCGAAATGGGGAAAGTTATAGATTTTCACAACACGTTGGTAAACAATATACCTACGGTAAAGGATTAGAAGAGCTGTCAGAAG
TAGAACAATTAAAATTAGAAAATAAGAGAAAAGATATAGAATTGGATATTTTAAAAAAGTACAAGGCATTGGAAAGGAAGTGGTACCAACAGTAGTCATA
GATTTAGTGGATCAATTAAAAGGAAACTATTCAATCAAATTGATACTAGAAGTATTAAATATACCTAAATCAACATATTACCGATGGAAAAACAAAACCT
ATAAAAATGATACTGTAACACAAAAAGTTATTGAATTATGTGAAGCTAACCATTATACCTACGGTTATCGTAAGATTACAGCACTGATTAATCAATGTTA
TACATCACCAATTAATCATAAGAGAGTACAGAGAATGATGCAGAAGCATCATTTGAACTGCCGAGTTAGACCTAAAAAGACGACAAGGATAGGTAAACCG
TATTATAAAACAGACAATTTATTACAAAGATAATTTAAAGCGAATTGTCCCATAGAAGTATTAACAACCGATATTACTTATTTACCATTTGATCATTCTA
TGTTGTATTTATCTTCGATAATGGATATTTATAACGGAGAAATTGTGGCGTATAAAATAGATGATAAACAAGACCAACGTTTAGTTAATGATACATTAAA
TCAAATCGATATACCTGAGGGGTATATATTACATAGTGATCAAGGCAGCGTTTATACATCTTATGCTTATTATCAATTATGCGAAGAAAAAGGCATTATC
AGAAGTATGTCCCGAAAGGGAACACCTGCCGATAACGCCCCGATAGAAAGTTTCCATTCCTCGCTAAAGTCTGAAACTCTTTACATCAATAATCAGCTTA
ATAGCTCTAATCATATTGTAATAGATATTGTCGAAAAGTACATTAAAAACTATAATAATAATCAAATTCAACAAAAACTAGGCTACTTATCCCCTGTAAA
ATACAGAGAATTAATAGCCTAGAACATGGTGTTTTTATTAAGTTCCCGTTTTAGGGGTTCA

Recoding section

Recoding by frameshift
Frame
Type
Experimentally demonstrated

Stimulators :

Shine-Dalgarno sequence :
Secondary structure :

Recoding motif :

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
315 bp	104 aa	81	395	+	No

ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MKRVSYSVETKYKAVEMKAAGFSTKEIMKELNIKNRTQVETWWRWYRNGESYRFSQHVGKQYTYGKGLEELSEVEQLKLENKRKDIELDILKKYKALERK
WYQQ

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
843 bp	298 aa	380	1222	+	No

ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

EKRYRIGYFKKVQGIGKEVVPTVVIDLVDQLKGNYSIKLILEVLNIPKSTYYRWKNKTYKNDTVTQKVIELCEANHYTYGYRKITALINQCYTSPINHKR
VQRMMQKHHLNCRVRPKKTTRIGKPYYKTDNLLQR*FKANCPIEVLTTDITYLPFDHSMLYLSSIMDIYNGEIVAYKIDDKQDQRLVNDTLNQIDIPEGY
ILHSDQGSVYTSYAYYQLCEEKGIIRSMSRKGTPADNAPIESFHSSLKSETLYINNQLNSSNHIVIDIVEKYIKNYNNNQIQQKLGYLSPVKYRELIA

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1142 bp	380 aa	81	1222	+	Yes

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKRVSYSVETKYKAVEMKAAGFSTKEIMKELNIKNRTQVETWWRWYRNGESYRFSQHVGKQYTYGKGLEELSEVEQLKLENKRKDIELDILKKVQGIGKE
VVPTVVIDLVDQLKGNYSIKLILEVLNIPKSTYYRWKNKTYKNDTVTQKVIELCEANHYTYGYRKITALINQCYTSPINHKRVQRMMQKHHLNCRVRPKK
TTRIGKPYYKTDNLLQR*FKANCPIEVLTTDITYLPFDHSMLYLSSIMDIYNGEIVAYKIDDKQDQRLVNDTLNQIDIPEGYILHSDQGSVYTSYAYYQL
CEEKGIIRSMSRKGTPADNAPIESFHSSLKSETLYINNQLNSSNHIVIDIVEKYIKNYNNNQIQQKLGYLSPVKYRELIA

Blast result :

Comments

ISSau4 is 49% aa similar to IS1076(ORF A) and 55% aa similar to IS150 (ORF B). The transposase is probably inactive : there is a codon STOP at position 136 of the ORF B.
There are 1 full copy in Staphylococcus aureus subsp. aureus MRSA252 genome, in Staphylococcus aureus subsp. aureus Mu50 and in Staphylococcus aureus subsp. aureus N315 ; and 1 full and 2 partial copies in Staphylococcus epidermidis ATCC 12228 .

References

1] Holden,M.T.G., Feil,E.J., Lindsay,J.A., Peacock,S.J., Day,N.P.J., Enright,M.C., Foster,T.J., Moore,C.E., Hurst,L., Atkin,R., Barron,A., Bason,N., Bentley,S.D., Chillingworth,C., Chillingworth,T., Churcher,C., Clark,L., Corton,C., Cronin,A., Doggett,J., Dowd,L., Feltwell,T., Hance,Z., Harris,B., Hauser,H., Holroyd,S., Jagels,K., James,K.D., Lennard,N., Line,A., Mayes,R., Moule,S., Mungall,K., Ormond,D., Quail,M.A., Rabbinowitsch,E., Rutherford,K., Sanders,M., Sharp,S., Simmonds,M., Stevens,K., Whitehead,S., Barrell,B.G., Spratt,B.G. and Parkhill,J. (2004) Proc. Natl. Acad. Sci. U.S.A. 101 (26), 9786-9791