ISSau2
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_002952 | ND | Staphylococcus aureus | Staphylococcus aureus subsp. aureus MRSA252 |
DNA section
IS Length : 1660 bp
Ends
IR Length : 29/40
IRL : TGAAATGCTCCCTTCAAAGTAGACATTGAAAAAATGAAAACTTTGAAGGG
IRR : TGAACTGCACCCAGTCTAGTAGACAATTAAATAATTAAAATTAATGGTTG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GCGATGAGCCG | CAT | TGAGACCGCAAGG | 3 |
DNA sequence
TGAAATGCTCCCTTCAAAGTAGACATTGAAAAAATGAAAACTTTGAAGGGAGCTTTATTTATGTCAATAAAACCTAAAGTAGACATAGATGTACTGCTAT
ATATGTTTCAAGAATATGAAAAAGGCGTGTCGTTTCAATACTTAATCGATACATTACAATTAGATATTAATGTCAATACTTTGTATCCTAAGTATAAATA
TTATAAAACCCATGGTATTGAAACACTACTTACACAAAAACAATACAATCACTATTCAAAGGAACTAAAATTAAAAGTTGTAAATGAATATTTGAATTCA
AATCAATCAACCCAAGACATCGCAATCAAATACAATATTCGTAGTTCGACACAAGTCAAAAATTGGATAAAAATGTATACTGTAGGAAAAGAGATTAAGA
ATCAATCTCCTAAAACTGGGGTGTATATCATGAAAGCAAGAAAAACCACATTTGAAGAACGTGTAACAATTGTTGAATATTACTTGAATCATAAACAATC
TTATAGAGAAGTTGCAGAACACTTTAATATCTCATACGGTCAAATTTACCAGTGGGTTCATAAGTATCAAGCACATGGTAAAAATGGATTAGTAGATGGC
AGAGGCAAAGGAAAACCCAAATCTATGATGACGCCAGAAGAACAAAAAGAAGCTGAAATTCAAGCATTAAAAGCACAAAATCGATTATTAGAGATGGAAA
ATGACGTTTTAAAAAAGTTTCAAGCATTGGAAAGAGAGATGATTCAAAGAGAAAACAAATCACGGCATACAAAACGATCGAAGCGTTAAAACATCAATAC
CCTATCCAACGCTTGTGTCGTATTTTAGGCATTTCTAGAGCGAGTTATTATAAGTGGGTACATTATCAATCGAGTGAACTTGAATTAGAGAATGAACAGC
TTAAGCGAGAAATAGAATCAATCTATCATAAGTATAATGGTATTTATGGCTATCGTCGCATTTATATCTATATTCGCTTAAAACTAGGTAAACAAGTAAA
CCGAAAGCGAATTTATCGTTTGATGAAAGAACTTAATTTAAAAGCAGTAATACGTCAAAAGCGAAAACCTTATCGACGTTCAACGCCACAAATCACATCA
GAAAATAAGCTTAATCGACAATTTGACATTGATACGCCAAATAAAGTTTGGTTAACAGATGTAACAGAATTTAAAATCAAAGAAGGTAGTAAGATATATT
TGAGTGCCATTTATGACTTAGGTGCTAAACGTATTGTTAGTTATGAGCTAGGCCCTTCTAACAATAATCAATTAGTATTTAAAACATTCAATCAAGCGAT
AGAAAAAGTAGAAAATACAAAAGGTATTTTATTTCATAGTGACCGAGGTTTTCAATATACATCGAAAACATTTAAACATATGTTGGATGAATGTGGCATG
ATTCAAAGTATGTCACGTGTTAGCAAATGTATCGATAACGGTCCAATGGAAGGTGTATGGGGTACAATAAAAAGTGAAATTTTTAGAGGAAATAAGCACT
TTAAATTTAATAGTGTAGAAGAAGCTACAAAAACAATTCATGATTTTATTCTTTTCTTTAATCATGAAAGAATTACATTAAAAATGGCTGATTCTGTATA
ACAACAAAACCAACCATTAATTTTAATTATTTAATTGTCTACTAGACTGGGTGCAGTTCA
ATATGTTTCAAGAATATGAAAAAGGCGTGTCGTTTCAATACTTAATCGATACATTACAATTAGATATTAATGTCAATACTTTGTATCCTAAGTATAAATA
TTATAAAACCCATGGTATTGAAACACTACTTACACAAAAACAATACAATCACTATTCAAAGGAACTAAAATTAAAAGTTGTAAATGAATATTTGAATTCA
AATCAATCAACCCAAGACATCGCAATCAAATACAATATTCGTAGTTCGACACAAGTCAAAAATTGGATAAAAATGTATACTGTAGGAAAAGAGATTAAGA
ATCAATCTCCTAAAACTGGGGTGTATATCATGAAAGCAAGAAAAACCACATTTGAAGAACGTGTAACAATTGTTGAATATTACTTGAATCATAAACAATC
TTATAGAGAAGTTGCAGAACACTTTAATATCTCATACGGTCAAATTTACCAGTGGGTTCATAAGTATCAAGCACATGGTAAAAATGGATTAGTAGATGGC
AGAGGCAAAGGAAAACCCAAATCTATGATGACGCCAGAAGAACAAAAAGAAGCTGAAATTCAAGCATTAAAAGCACAAAATCGATTATTAGAGATGGAAA
ATGACGTTTTAAAAAAGTTTCAAGCATTGGAAAGAGAGATGATTCAAAGAGAAAACAAATCACGGCATACAAAACGATCGAAGCGTTAAAACATCAATAC
CCTATCCAACGCTTGTGTCGTATTTTAGGCATTTCTAGAGCGAGTTATTATAAGTGGGTACATTATCAATCGAGTGAACTTGAATTAGAGAATGAACAGC
TTAAGCGAGAAATAGAATCAATCTATCATAAGTATAATGGTATTTATGGCTATCGTCGCATTTATATCTATATTCGCTTAAAACTAGGTAAACAAGTAAA
CCGAAAGCGAATTTATCGTTTGATGAAAGAACTTAATTTAAAAGCAGTAATACGTCAAAAGCGAAAACCTTATCGACGTTCAACGCCACAAATCACATCA
GAAAATAAGCTTAATCGACAATTTGACATTGATACGCCAAATAAAGTTTGGTTAACAGATGTAACAGAATTTAAAATCAAAGAAGGTAGTAAGATATATT
TGAGTGCCATTTATGACTTAGGTGCTAAACGTATTGTTAGTTATGAGCTAGGCCCTTCTAACAATAATCAATTAGTATTTAAAACATTCAATCAAGCGAT
AGAAAAAGTAGAAAATACAAAAGGTATTTTATTTCATAGTGACCGAGGTTTTCAATATACATCGAAAACATTTAAACATATGTTGGATGAATGTGGCATG
ATTCAAAGTATGTCACGTGTTAGCAAATGTATCGATAACGGTCCAATGGAAGGTGTATGGGGTACAATAAAAAGTGAAATTTTTAGAGGAAATAAGCACT
TTAAATTTAATAGTGTAGAAGAAGCTACAAAAACAATTCATGATTTTATTCTTTTCTTTAATCATGAAAGAATTACATTAAAAATGGCTGATTCTGTATA
ACAACAAAACCAACCATTAATTTTAATTATTTAATTGTCTACTAGACTGGGTGCAGTTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 756 bp | 251 aa | 34 | 789 | + | No |
Description : First part of the transposase
ORF sequence :
MKTLKGALFMSIKPKVDIDVLLYMFQEYEKGVSFQYLIDTLQLDINVNTLYPKYKYYKTHGIETLLTQKQYNHYSKELKLKVVNEYLNSNQSTQDIAIKY
NIRSSTQVKNWIKMYTVGKEIKNQSPKTGVYIMKARKTTFEERVTIVEYYLNHKQSYREVAEHFNISYGQIYQWVHKYQAHGKNGLVDGRGKGKPKSMMT
PEEQKEAEIQALKAQNRLLEMENDVLKKFQALEREMIQRENKSRHTKRSKR
NIRSSTQVKNWIKMYTVGKEIKNQSPKTGVYIMKARKTTFEERVTIVEYYLNHKQSYREVAEHFNISYGQIYQWVHKYQAHGKNGLVDGRGKGKPKSMMT
PEEQKEAEIQALKAQNRLLEMENDVLKKFQALEREMIQRENKSRHTKRSKR
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 897 bp | 297 aa | 705 | 1601 | + | No |
Description : Second part of the transposase
ORF sequence :
RFKKVSSIGKRDDSKRKQITAYKTIEALKHQYPIQRLCRILGISRASYYKWVHYQSSELELENEQLKREIESIYHKYNGIYGYRRIYIYIRLKLGKQVNR
KRIYRLMKELNLKAVIRQKRKPYRRSTPQITSENKLNRQFDIDTPNKVWLTDVTEFKIKEGSKIYLSAIYDLGAKRIVSYELGPSNNNQLVFKTFNQAIE
KVENTKGILFHSDRGFQYTSKTFKHMLDECGMIQSMSRVSKCIDNGPMEGVWGTIKSEIFRGNKHFKFNSVEEATKTIHDFILFFNHERITLKMADS
KRIYRLMKELNLKAVIRQKRKPYRRSTPQITSENKLNRQFDIDTPNKVWLTDVTEFKIKEGSKIYLSAIYDLGAKRIVSYELGPSNNNQLVFKTFNQAIE
KVENTKGILFHSDRGFQYTSKTFKHMLDECGMIQSMSRVSKCIDNGPMEGVWGTIKSEIFRGNKHFKFNSVEEATKTIHDFILFFNHERITLKMADS
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1568 bp | 521 aa | 34 | 1601 | + | Yes |
Chemistry : DDE
ORF sequence :
MKTLKGALFMSIKPKVDIDVLLYMFQEYEKGVSFQYLIDTLQLDINVNTLYPKYKYYKTHGIETLLTQKQYNHYSKELKLKVVNEYLNSNQSTQDIAIKY
NIRSSTQVKNWIKMYTVGKEIKNQSPKTGVYIMKARKTTFEERVTIVEYYLNHKQSYREVAEHFNISYGQIYQWVHKYQAHGKNGLVDGRGKGKPKSMMT
PEEQKEAEIQALKAQNRLLEMENDVLKKVSSIGKRDDSKRKQITAYKTIEALKHQYPIQRLCRILGISRASYYKWVHYQSSELELENEQLKREIESIYHK
YNGIYGYRRIYIYIRLKLGKQVNRKRIYRLMKELNLKAVIRQKRKPYRRSTPQITSENKLNRQFDIDTPNKVWLTDVTEFKIKEGSKIYLSAIYDLGAKR
IVSYELGPSNNNQLVFKTFNQAIEKVENTKGILFHSDRGFQYTSKTFKHMLDECGMIQSMSRVSKCIDNGPMEGVWGTIKSEIFRGNKHFKFNSVEEATK
TIHDFILFFNHERITLKMADS
NIRSSTQVKNWIKMYTVGKEIKNQSPKTGVYIMKARKTTFEERVTIVEYYLNHKQSYREVAEHFNISYGQIYQWVHKYQAHGKNGLVDGRGKGKPKSMMT
PEEQKEAEIQALKAQNRLLEMENDVLKKVSSIGKRDDSKRKQITAYKTIEALKHQYPIQRLCRILGISRASYYKWVHYQSSELELENEQLKREIESIYHK
YNGIYGYRRIYIYIRLKLGKQVNRKRIYRLMKELNLKAVIRQKRKPYRRSTPQITSENKLNRQFDIDTPNKVWLTDVTEFKIKEGSKIYLSAIYDLGAKR
IVSYELGPSNNNQLVFKTFNQAIEKVENTKGILFHSDRGFQYTSKTFKHMLDECGMIQSMSRVSKCIDNGPMEGVWGTIKSEIFRGNKHFKFNSVEEATK
TIHDFILFFNHERITLKMADS
Blast result :
Comments
ISSau2 is 50%(ORF1) aa similar to IS1223 and 59%(ORF2) to ISPsy8.
There are 5 copies in Staphylococcus aureus subsp. aureus MRSA252 and none in Staphylococcus aureus subsp. aureus MSSA476.
There are 5 copies in Staphylococcus aureus subsp. aureus MRSA252 and none in Staphylococcus aureus subsp. aureus MSSA476.
References
1] Holden,M.T.G., Feil,E.J., Lindsay,J.A., Peacock,S.J., Day,N.P.J., Enright,M.C., Foster,T.J., Moore,C.E., Hurst,L., Atkin,R.,Barron,A., Bason,N., Bentley,S.D., Chillingworth,C., Chillingworth,T., Churcher,C., Clark,L., Corton,C., Cronin,A., Doggett,J., Dowd,L., Feltwell,T., Hance,Z., Harris,B., Hauser,H., Holroyd,S., Jagels,K., James,K.D., Lennard,N., Line,A., Mayes,R., Moule,S., Mungall,K., Ormond,D., Quail,M.A., Rabbinowitsch,E., Rutherford,K., Sanders,M., Sharp,S., Simmonds,M., Stevens,K., Whitehead,S., Barrell,B.G., Spratt,B.G. and Parkhill,J. (2004) Proc. Natl. Acad. Sci. U.S.A. 101 (26), 9786-9791