ISTfe1

  • Family ISL3
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s) IST1, ISAfe1
Accession numberTranspositionOriginHost
U66426 Y Acidithiobacillus ferrooxidans
Acidithiobacillus ferrooxidans ATCC 19859
DNA section
IS Length : 1303 bp

Ends


IR Length : 21/26

IRL : GGCTCTTCGTCGGATTGAGTGGGTAGGGGGTAGAATGCTGTGGAGCAGTT
IRR : GGCTCTTCGTCATTTCAAGTGGGTAGCCTGAGATCCAGCTTACCCAGGAT

Insertion site


Left flankDirect repeatRight flankDR Length

DNA sequence

GGCTCTTCGTCGGATTGAGTGGGTAGGGGGTAGAATGCTGTGGAGCAGTTGCGTTCCCGAATAGGAGTTAGCTGATGGTCCCAGAAGATCTGTTTTCTCT
CGCGTTAGGGTTGGTACCGCCGTGGTTGGTGGACGATGTGACCTTCCAAGTGGAGGAGAAGCGCCTGGATCTGCACATCAACTTTCCCAAGGGCAGTCGC
TTTGCTTGCCCCGTCTGTGGTGAGGAGTGTCCGGTACATGACACCCGTGAACATACCTGGCGGCACATGGATTTCTTTCAGCATGAAGCCTATCTCCACG
CCCGCGTACCCCGTGTGATGTGCCCGGAGCATGGGGTGCATCAGATCCCTGTTCCCTGGGCGCGGGAAGGCTCGCGTTTCACCCTGCTCTTTGAAGCGCT
GATCATGACCCTGGTGCGGGAGATGCCAGTATTGACGGTAGCCCGCTTGGTCGGCGAGACCGACACGCTCCTGTGGCGGGTGATTGACCATTATGTGCCC
GAAGCCCGTACCAGGGTGGATATGGCCAATGTCCATGCCGTCGGCGTCGATGAGACCAGCAGTCGGCGCGGTCATGACTACATCACGCTCTTCGTGGATC
TGAATGCCCGGCGACTCTTGTTCGCTACGCCCGGCAAGGATGCCAAGACCTTTGAGAAATTCTCCGAAGATCTACAGGCCCATGGTGGTAGCGCGGAAGC
GATCACCGATGTGAGCATGGACCTCTCGCCGGCCTTCCAGAAAGGGGCTGCCGAGCACCTGCCCAATGCTGAGATCACCTTCGATCGTTTTCACCTCATG
AAGCTCGTCAACGAGGCCGTGGATGCCGTGCGCAAGGGGGAAGTCCTCACCCAGCCAAATCTCAAAAAGACCCGCTGGCTCTGGCTCAAGAATGATTGTA
ACCTCAAGGCCAGGCAGAAAGAAAAGCTGCAGGAATTACTCAAAGACCAGAACCTCAAAACGGCACAGGCCTACCAGTTTCGCCTGACTTTTCAGGACAT
CTTCACAGTCAAGAATCGCCACCAGGGCGCCACCCTGCTCAAGGCCTGGATGGAAAACGCCAAGGACAGCGGAATGCCGCCTATGGTCAAGGTCGCCTAC
ACCATCATGAACCATTGGGATGGCGTGCTCCGCTGGTTCGAAAGCCAGATTACCAATGGAATTCTGGAAGGCTTCAACAGCCTCATTCAATCCGCCAAGG
CCAAGGCCCGGGGTTACCGCACCCACAAGAATTTTATCAACATGGCCTACCTGATCCTGGGTAAGCTGGATCTCAGGCTACCCACTTGAAATGACGAAGA
GCC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1215 bp404 aa751289+No
ORF function : Transposase
Chemistry : Unknow

ORF sequence :

MVPEDLFSLALGLVPPWLVDDVTFQVEEKRLDLHINFPKGSRFACPVCGEECPVHDTREHTWRHMDFFQHEAYLHARVPRVMCPEHGVHQIPVPWAREGS
RFTLLFEALIMTLVREMPVLTVARLVGETDTLLWRVIDHYVPEARTRVDMANVHAVGVDETSSRRGHDYITLFVDLNARRLLFATPGKDAKTFEKFSEDL
QAHGGSAEAITDVSMDLSPAFQKGAAEHLPNAEITFDRFHLMKLVNEAVDAVRKGEVLTQPNLKKTRWLWLKNDCNLKARQKEKLQELLKDQNLKTAQAY
QFRLTFQDIFTVKNRHQGATLLKAWMENAKDSGMPPMVKVAYTIMNHWDGVLRWFESQITNGILEGFNSLIQSAKAKARGYRTHKNFINMAYLILGKLDL
RLPT

 

Blast result :
Comments
This IS had three names : IST1, ISTfe1 and ISAfe1. ISAfe1 had also been attributed to an IS1111 family member.

ISTfe1 is 59% aa similar to ISBlo6. There are 20 copies of this IS in the genome.
References
1] Holmes,D.S., Zhao,H.-L., Levican,G., Ratouchniak,J., Bonnefoy,V., Varela,P. and Jedlicki,E. (2001)J. Bacteriol. 183 (14), 4323-4329