ISMae3
- Family IS1
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AJ577139 | ND | Microcystis aeruginosa | Microcystis aeruginosa |
DNA section
IS Length : 834 bp
Ends
IR Length : 23/25
IRL : GGCGTTGCTGATTTAGGGTATGATTGAGGAGGAAGGCGATCACCCAAAAA
IRR : GGCGTTGCTGAATTAAGGTATGATTAGGCAAAATGGCGAGCATTCAGAGG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
GGCGTTGCTGATTTAGGGTATGATTGAGGAGGAAGGCGATCACCCAAAAATACCCATTATCAAACTCAGAAATTATGCAATGTCCTGAATGTCAATCCAA
TCATATCAATAAAAATGGTCATAAAAAAGGAAAACAGAACTATATTTGTGTGAACTGCGGAAGACAATTTATAGAGGTTTATGAACCCCAGAGAGGTTAT
TCAGAAGAAATGAAACGGGAATGTCTGAAAATGTATGTTAATGGTCTGGGTTTTCGAGCCATTGAAAGGGTTAAAAACGTTCATCATACGACAATTATGA
ATTGGGTTAAACAAGTCGGCGAACTTTTACCGGATTATTATGACCCACAAACAATTCCCGATGTGGGAGAATTAGATGAATTAGAAACATTTGTCGGCTC
AAAAAAAACAAAATCTGGTTATGGACAGCGGTAGATCACTTTAAAACAGGGATTTTAGGATGGGTTTTAGGCGATCATAGTGCTAAAACTTTTGCGGACT
TATGGAAAGTTGTTGGTCAGTGGAAATGTCATTTTTATGTGACAGATGGATGGAAAGTTTACCCTAATTTTATCCCCGACGGAGACCAGATTATTAGCAA
AACTTATATGACCAGAGTAGAGGGAGAAAATACTAGACTAAGACATTATTTAGCCAGGTTACATCGAAAAACTCTTTGTTATTCCAAGTCTGAACAAATG
CTCAGACATTCAATTCGATTACTAATTCACTACCTAAAATTTGAGGATGTTCCAGTTCCTTATTCAAATAACAGAAGTTATTTTCCTCTGAATGCTCGCC
ATTTTGCCTAATCATACCTTAATTCAGCAACGCC
TCATATCAATAAAAATGGTCATAAAAAAGGAAAACAGAACTATATTTGTGTGAACTGCGGAAGACAATTTATAGAGGTTTATGAACCCCAGAGAGGTTAT
TCAGAAGAAATGAAACGGGAATGTCTGAAAATGTATGTTAATGGTCTGGGTTTTCGAGCCATTGAAAGGGTTAAAAACGTTCATCATACGACAATTATGA
ATTGGGTTAAACAAGTCGGCGAACTTTTACCGGATTATTATGACCCACAAACAATTCCCGATGTGGGAGAATTAGATGAATTAGAAACATTTGTCGGCTC
AAAAAAAACAAAATCTGGTTATGGACAGCGGTAGATCACTTTAAAACAGGGATTTTAGGATGGGTTTTAGGCGATCATAGTGCTAAAACTTTTGCGGACT
TATGGAAAGTTGTTGGTCAGTGGAAATGTCATTTTTATGTGACAGATGGATGGAAAGTTTACCCTAATTTTATCCCCGACGGAGACCAGATTATTAGCAA
AACTTATATGACCAGAGTAGAGGGAGAAAATACTAGACTAAGACATTATTTAGCCAGGTTACATCGAAAAACTCTTTGTTATTCCAAGTCTGAACAAATG
CTCAGACATTCAATTCGATTACTAATTCACTACCTAAAATTTGAGGATGTTCCAGTTCCTTATTCAAATAACAGAAGTTATTTTCCTCTGAATGCTCGCC
ATTTTGCCTAATCATACCTTAATTCAGCAACGCC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 360 bp | 119 aa | 75 | 434 | + | No |
Description : First part of the transposase
ORF sequence :
MQCPECQSNHINKNGHKKGKQNYICVNCGRQFIEVYEPQRGYSEEMKRECLKMYVNGLGFRAIERVKNVHHTTIMNWVKQVGELLPDYYDPQTIPDVGEL
DELETFVGSKKTKSGYGQR
DELETFVGSKKTKSGYGQR
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 432 bp | 143 aa | 380 | 811 | + | No |
Description : Second part of the transposase
ORF sequence :
IRNICRLKKNKIWLWTAVDHFKTGILGWVLGDHSAKTFADLWKVVGQWKCHFYVTDGWKVYPNFIPDGDQIISKTYMTRVEGENTRLRHYLARLHRKTLC
YSKSEQMLRHSIRLLIHYLKFEDVPVPYSNNRSYFPLNARHFA
YSKSEQMLRHSIRLLIHYLKFEDVPVPYSNNRSYFPLNARHFA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 737 bp | 245 aa | 75 | 811 | + | Yes |
Chemistry : DDE
ORF sequence :
MQCPECQSNHINKNGHKKGKQNYICVNCGRQFIEVYEPQRGYSEEMKRECLKMYVNGLGFRAIERVKNVHHTTIMNWVKQVGELLPDYYDPQTIPDVGEL
DELETFVGSKKTKIWLWTAVDHFKTGILGWVLGDHSAKTFADLWKVVGQWKCHFYVTDGWKVYPNFIPDGDQIISKTYMTRVEGENTRLRHYLARLHRKT
LCYSKSEQMLRHSIRLLIHYLKFEDVPVPYSNNRSYFPLNARHFA
DELETFVGSKKTKIWLWTAVDHFKTGILGWVLGDHSAKTFADLWKVVGQWKCHFYVTDGWKVYPNFIPDGDQIISKTYMTRVEGENTRLRHYLARLHRKT
LCYSKSEQMLRHSIRLLIHYLKFEDVPVPYSNNRSYFPLNARHFA
Blast result :
Comments
ISMae3 is 84% aa similar to ISNpu9.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Mlouka,A., Comte,K., Castets,A.M., Bouchier,C. and Tandeau De Marsac,N.(2004) J. Bacteriol. 186 (8), 2355-2365