ISPsy7
- Family IS110
- Group IS1111
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AE016867 | ND | Pseudomonas syringae | Pseudomonas syringae pv. tomato DC3000 |
DNA section
IS Length : 1348 bp
Ends
IR Length : 11
IRL : taatgagATGGTCAGCCCGATCCGAGAAACCCGGAGTGTCTACACTTTCC
IRR : ----tgtATGGTCAGCCCACCTCCTGCAATCGCAGTTCGATGAGCGGTAA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
TAATGAGATGGTCAGCCCGATCCGAGAAACCCGGAGTGTCTACACTTTCCGGGTTTCTCTCTAACGTCGAGGAGATCACTCATGAAAAACGGCCTCAAGC
TCTTAGGCATCGACATTGGTAAAAACAGTTTTCACCTCATAGGCCATGATGCAGGCGGCCACCAAGTATTACGCAAGTGCTTCAATCGCAATCGCCTTCT
GCAGTTTGCGGCTCAGATTGAACCCTGCACCATTGCAATGGAGTCGTGCGGTGGCTCTCACTGGCTTGCCAGGAAACTCGCCTCGCTGGGGCACCGGGTG
AAGTTGATTGCTCCGCAACATGTGAAGCCCTACGTGACGGGCAACAAGAATGACTTCATCGATGCCGAAGCTATATGCGAGGCGGCCTCCAGACCACGTA
CTCATTACGTTGCCGTCAAGACTGTGGAGCAGCAGGTACTTTCTGTACAACATCGTCTTCGCGAGTCACTGGTTTGCCACCGTACTGAAGCCATCAACAG
GGTGCATGGTTTTTTGCTTGAGTTCGGAATTGCCTTGCCAGCCACCAAGGCAGCGATCGCCAAAGTGCCTGAGCTGATTGACGATTCACGTAGCGACCTG
CCGCTGCAGTTCAAAAGGGTCATGGAGCATTTGCTGGAGGATATTCATCGCCTTGATACCGAAATAAAGGCCTGTGATGCGCAAATCAAGCAGCAACTGG
CGCAAGACGATGCTGGTACACGCTTGATGACGATTCCTGGCATTGGTCCTATTACCGCCAGCGCATTTGTAGCGGATCTGGGTGACGCTTCCAATTTTCG
AGCCTCACGAGACCTTTCCGCTTTTCTGGGGCTGGTTCCCAGACAGTTCTCTACAGGCGGGAGACCCGTATTGCTGGGTATCAGCAAGCGAGGTGACAGG
CACCTGCGAACCCTGTTGGTGCAAGGTTCTCGAGCGCTCATGATTCGAATAGAACGCCGCGATGATGCCCTTGGGCAGTGGGTAAGAGACTTGTTGGCAC
GCAAGCATCCGAACAAGGTGGCATGTGCTTTAGCGAACAAGCTGGCAAGAATCGTATGGGCGGTGCTCAGACGAGGTAGCACTTACAACGCATCAATGAT
CTGAGTAGGACATAGGCGTACCGGCTTTTGCGACGCAGTGATGATGACGATAAACAGCTCATGGCCAGACTGAAAACCTGATAGGCAAAGCAGCCTTCGA
GGCTTTAGGGATTTTTGAGGACAGTAAGGCGGCGAATACTCATCAAGGGCCGGGCAGCGAAATTGCCCAGAATGAGCCCGGATACATTAGCGCAAGCATG
CTTTACCGCTCATCGAACTGCGATTGCAGGAGGTGGGCTGACCATACA
TCTTAGGCATCGACATTGGTAAAAACAGTTTTCACCTCATAGGCCATGATGCAGGCGGCCACCAAGTATTACGCAAGTGCTTCAATCGCAATCGCCTTCT
GCAGTTTGCGGCTCAGATTGAACCCTGCACCATTGCAATGGAGTCGTGCGGTGGCTCTCACTGGCTTGCCAGGAAACTCGCCTCGCTGGGGCACCGGGTG
AAGTTGATTGCTCCGCAACATGTGAAGCCCTACGTGACGGGCAACAAGAATGACTTCATCGATGCCGAAGCTATATGCGAGGCGGCCTCCAGACCACGTA
CTCATTACGTTGCCGTCAAGACTGTGGAGCAGCAGGTACTTTCTGTACAACATCGTCTTCGCGAGTCACTGGTTTGCCACCGTACTGAAGCCATCAACAG
GGTGCATGGTTTTTTGCTTGAGTTCGGAATTGCCTTGCCAGCCACCAAGGCAGCGATCGCCAAAGTGCCTGAGCTGATTGACGATTCACGTAGCGACCTG
CCGCTGCAGTTCAAAAGGGTCATGGAGCATTTGCTGGAGGATATTCATCGCCTTGATACCGAAATAAAGGCCTGTGATGCGCAAATCAAGCAGCAACTGG
CGCAAGACGATGCTGGTACACGCTTGATGACGATTCCTGGCATTGGTCCTATTACCGCCAGCGCATTTGTAGCGGATCTGGGTGACGCTTCCAATTTTCG
AGCCTCACGAGACCTTTCCGCTTTTCTGGGGCTGGTTCCCAGACAGTTCTCTACAGGCGGGAGACCCGTATTGCTGGGTATCAGCAAGCGAGGTGACAGG
CACCTGCGAACCCTGTTGGTGCAAGGTTCTCGAGCGCTCATGATTCGAATAGAACGCCGCGATGATGCCCTTGGGCAGTGGGTAAGAGACTTGTTGGCAC
GCAAGCATCCGAACAAGGTGGCATGTGCTTTAGCGAACAAGCTGGCAAGAATCGTATGGGCGGTGCTCAGACGAGGTAGCACTTACAACGCATCAATGAT
CTGAGTAGGACATAGGCGTACCGGCTTTTGCGACGCAGTGATGATGACGATAAACAGCTCATGGCCAGACTGAAAACCTGATAGGCAAAGCAGCCTTCGA
GGCTTTAGGGATTTTTGAGGACAGTAAGGCGGCGAATACTCATCAAGGGCCGGGCAGCGAAATTGCCCAGAATGAGCCCGGATACATTAGCGCAAGCATG
CTTTACCGCTCATCGAACTGCGATTGCAGGAGGTGGGCTGACCATACA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1023 bp | 340 aa | 82 | 1104 | + | No |
Chemistry : DEDD
ORF sequence :
MKNGLKLLGIDIGKNSFHLIGHDAGGHQVLRKCFNRNRLLQFAAQIEPCTIAMESCGGSHWLARKLASLGHRVKLIAPQHVKPYVTGNKNDFIDAEAICE
AASRPRTHYVAVKTVEQQVLSVQHRLRESLVCHRTEAINRVHGFLLEFGIALPATKAAIAKVPELIDDSRSDLPLQFKRVMEHLLEDIHRLDTEIKACDA
QIKQQLAQDDAGTRLMTIPGIGPITASAFVADLGDASNFRASRDLSAFLGLVPRQFSTGGRPVLLGISKRGDRHLRTLLVQGSRALMIRIERRDDALGQW
VRDLLARKHPNKVACALANKLARIVWAVLRRGSTYNASMI
AASRPRTHYVAVKTVEQQVLSVQHRLRESLVCHRTEAINRVHGFLLEFGIALPATKAAIAKVPELIDDSRSDLPLQFKRVMEHLLEDIHRLDTEIKACDA
QIKQQLAQDDAGTRLMTIPGIGPITASAFVADLGDASNFRASRDLSAFLGLVPRQFSTGGRPVLLGISKRGDRHLRTLLVQGSRALMIRIERRDDALGQW
VRDLLARKHPNKVACALANKLARIVWAVLRRGSTYNASMI
Blast result :
Comments
Additionnal Accession number : AE016853.
The IR of this IS are not at its termini. In the IS sequence as given 7 nt separate IRl from the left-hand end of the element and 3 nt separate IRr from the right-hand end. The first residue of the sequence may in fact belong as the final residue, giving 6 nt on the left and 4 on the right. Some uninterrupted target sequences predict that the first T of the given sequence is missing, giving terminal sequences totalling 9 rather than 10 bp.
The transposase protein is 72% identical to that of ISPfl1 and 39% identical to that of IS1111.
There are 10 copies of ISPsy7 in the chromosome of this strain. It is difficult to define the boundary of the right end-sequence since ISPsy7 appears to lack an inverted repeat and a direct repeat, and the element might preferentially insert into an imprecise DNA repeat.
There are 10 copies of ISPsy7 in Pseudomonas syringae pv. tomato DC3000 genome.
By analogy with IS4321, ISPsy7 may exist in a circular form. If the terminal sequences total 10 bp a -10 region is created by these abutted sequences and is at an appropriate distance from a -35 region located just inside the right-hand end of the element to form a promoter.
The IR of this IS are not at its termini. In the IS sequence as given 7 nt separate IRl from the left-hand end of the element and 3 nt separate IRr from the right-hand end. The first residue of the sequence may in fact belong as the final residue, giving 6 nt on the left and 4 on the right. Some uninterrupted target sequences predict that the first T of the given sequence is missing, giving terminal sequences totalling 9 rather than 10 bp.
The transposase protein is 72% identical to that of ISPfl1 and 39% identical to that of IS1111.
There are 10 copies of ISPsy7 in the chromosome of this strain. It is difficult to define the boundary of the right end-sequence since ISPsy7 appears to lack an inverted repeat and a direct repeat, and the element might preferentially insert into an imprecise DNA repeat.
There are 10 copies of ISPsy7 in Pseudomonas syringae pv. tomato DC3000 genome.
By analogy with IS4321, ISPsy7 may exist in a circular form. If the terminal sequences total 10 bp a -10 region is created by these abutted sequences and is at an appropriate distance from a -35 region located just inside the right-hand end of the element to form a promoter.
References
1] Buell et al. (2003) Proc. Natl. Acad. Sci. U.S.A. 100, 10181-10186
2] Partridge and Hall (2003) J. Bacteriol. 185, 6371-6384
2] Partridge and Hall (2003) J. Bacteriol. 185, 6371-6384