ISPfl1
- Family IS110
- Group IS1111
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Pseudomonas fluorescens | Pseudomonas fluorescens Pf0-1 |
DNA section
IS Length : 1342 bp
Ends
IR Length : 12
IRL : taatgagATGGTCAGCCCAGTACGGGAACCCCGCTAAATCTAAACTTATC
IRR : ----tgtATGGTCAGCCCATCTCCTGCAATCACGAGCGATGAACGGTAAA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
TAATGAGATGGTCAGCCCAGTACGGGAACCCCGCTAAATCTAAACTTATCGGGGTTCTCACTGACTTGCGGAGACCTCTCATGGACACCTTGAACTTGCT
CGGCCTGGATATTGGGAAAAGCACCTTTCATCTTGTAGGGCACGACGTTAAAGGCAAGGAAGTGTTGCGCAAGAAATTCAATCGCAACCGCTTGCTCGAG
TTCGCAGCAAGGCTGAAGCCCTGCACCATAGCGATGGAGTCTTGTGGCGGTTCACACTGGCTTGCCAGAAAGCTCTTGTCTTTCGGTCATCAAGTGAAAT
TGATTGCGCCGCAGCATGTAAAACCTTACGTCACCGGCAACAAGAACGACTACATCGACGCTGAGGCAATTTGCGAAGCCGCATCTCGCCCTAAAACACG
TTACGTCGCCATCAAAACCGTTGAACAGCAGGTGCTATCAACCCAGCACCGCCTTCGTGAGGCGTTGGTAGGACACCGTACTCAAACGATCAATCGCATT
CACGGGTTCTTGCTTGAGTTCGGGATCGAACTGCCTGCAACAAAAGCGGCTATGAATCAAGTTCATCAAATTCTGGATGACCCTGCCGCCGAGTTACCGC
AACAGCTGAAGTCTTTTATTGAACATCTACTGGAAGAGATGAAGCGGCTCAACGACGAGATTAAGGCGATTGACCGGAGTATCAAGGAGCAAGTGGACAC
TGATGACGCGGGAACACGCCTGATGAGCATCCCCGGCATCGGCCCGATCATCGCTAGCGCGCTGCTGGCCGACGTCGGCGACGCTTCCAATTTTCACTCA
TCGCGAGACTTCGCCGCTTCGCTGGGACTGGTGCCAAGGCAATATTCAACAGGCGACCAAACCGTCCTTTTGAACATCAGCAAGCGTGGTGATAAATACC
TTCGAAAGCTTATGATTCAAGGCTCGCGTGCAGTTCTGGTGCACATAGATAAACGTACCGATGCTTTGGGAACCTGGTGCAGAGACCTGCTGATACGAAA
GCACCCGAATAAAGTCGCCTGCGCTTTGGCCAATAAATTGGCAAGGATCGTATGGGCTGTTTTAGCTCGCGGAGGTAGCTACAACGCACAATCCATGGCT
TGACCGAAATTCACTACCGGCTTTTGCGACGACAAGATGATGACGATAAACAGCTCAACTCCTGCTTGAAAACCTGGCAAACGAATCAGCTTTCGAGGCT
GGCACCCTTTTCAAGGACAGGCAGGAGCGACTACTCATCAAGGGCCAGGTGGCAACACTGCCGACCATGAGCCCGGATACATTAGCGCAAGCATGCTTTA
CCGTTCATCGCTCGTGATTGCAGGAGATGGGCTGACCATACA
CGGCCTGGATATTGGGAAAAGCACCTTTCATCTTGTAGGGCACGACGTTAAAGGCAAGGAAGTGTTGCGCAAGAAATTCAATCGCAACCGCTTGCTCGAG
TTCGCAGCAAGGCTGAAGCCCTGCACCATAGCGATGGAGTCTTGTGGCGGTTCACACTGGCTTGCCAGAAAGCTCTTGTCTTTCGGTCATCAAGTGAAAT
TGATTGCGCCGCAGCATGTAAAACCTTACGTCACCGGCAACAAGAACGACTACATCGACGCTGAGGCAATTTGCGAAGCCGCATCTCGCCCTAAAACACG
TTACGTCGCCATCAAAACCGTTGAACAGCAGGTGCTATCAACCCAGCACCGCCTTCGTGAGGCGTTGGTAGGACACCGTACTCAAACGATCAATCGCATT
CACGGGTTCTTGCTTGAGTTCGGGATCGAACTGCCTGCAACAAAAGCGGCTATGAATCAAGTTCATCAAATTCTGGATGACCCTGCCGCCGAGTTACCGC
AACAGCTGAAGTCTTTTATTGAACATCTACTGGAAGAGATGAAGCGGCTCAACGACGAGATTAAGGCGATTGACCGGAGTATCAAGGAGCAAGTGGACAC
TGATGACGCGGGAACACGCCTGATGAGCATCCCCGGCATCGGCCCGATCATCGCTAGCGCGCTGCTGGCCGACGTCGGCGACGCTTCCAATTTTCACTCA
TCGCGAGACTTCGCCGCTTCGCTGGGACTGGTGCCAAGGCAATATTCAACAGGCGACCAAACCGTCCTTTTGAACATCAGCAAGCGTGGTGATAAATACC
TTCGAAAGCTTATGATTCAAGGCTCGCGTGCAGTTCTGGTGCACATAGATAAACGTACCGATGCTTTGGGAACCTGGTGCAGAGACCTGCTGATACGAAA
GCACCCGAATAAAGTCGCCTGCGCTTTGGCCAATAAATTGGCAAGGATCGTATGGGCTGTTTTAGCTCGCGGAGGTAGCTACAACGCACAATCCATGGCT
TGACCGAAATTCACTACCGGCTTTTGCGACGACAAGATGATGACGATAAACAGCTCAACTCCTGCTTGAAAACCTGGCAAACGAATCAGCTTTCGAGGCT
GGCACCCTTTTCAAGGACAGGCAGGAGCGACTACTCATCAAGGGCCAGGTGGCAACACTGCCGACCATGAGCCCGGATACATTAGCGCAAGCATGCTTTA
CCGTTCATCGCTCGTGATTGCAGGAGATGGGCTGACCATACA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1023 bp | 340 aa | 81 | 1103 | + | No |
Chemistry : DEDD
ORF sequence :
MDTLNLLGLDIGKSTFHLVGHDVKGKEVLRKKFNRNRLLEFAARLKPCTIAMESCGGSHWLARKLLSFGHQVKLIAPQHVKPYVTGNKNDYIDAEAICEA
ASRPKTRYVAIKTVEQQVLSTQHRLREALVGHRTQTINRIHGFLLEFGIELPATKAAMNQVHQILDDPAAELPQQLKSFIEHLLEEMKRLNDEIKAIDRS
IKEQVDTDDAGTRLMSIPGIGPIIASALLADVGDASNFHSSRDFAASLGLVPRQYSTGDQTVLLNISKRGDKYLRKLMIQGSRAVLVHIDKRTDALGTWC
RDLLIRKHPNKVACALANKLARIVWAVLARGGSYNAQSMA
ASRPKTRYVAIKTVEQQVLSTQHRLREALVGHRTQTINRIHGFLLEFGIELPATKAAMNQVHQILDDPAAELPQQLKSFIEHLLEEMKRLNDEIKAIDRS
IKEQVDTDDAGTRLMSIPGIGPIIASALLADVGDASNFHSSRDFAASLGLVPRQYSTGDQTVLLNISKRGDKYLRKLMIQGSRAVLVHIDKRTDALGTWC
RDLLIRKHPNKVACALANKLARIVWAVLARGGSYNAQSMA
Blast result :
Comments
The IR of this IS are not at its termini. In the IS sequence as given 7 nt separate IRl from the left-hand end of the element and 3 nt separate IRr from the right-hand end. The first residue of the sequence may in fact belong as the final residue, giving 6 nt on the left and 4 on the right.
The transposase protein is 72% identical to that of ISPsy7 and 40% identical to that of IS1111.
By analogy with IS4321, ISPfl1 may exist in a circular form in which a -10 region created by the abutted terminal sequences and a -35 region located just inside the right-hand end of the element are correctly spaced to form a promoter.
The transposase protein is 72% identical to that of ISPsy7 and 40% identical to that of IS1111.
By analogy with IS4321, ISPfl1 may exist in a circular form in which a -10 region created by the abutted terminal sequences and a -35 region located just inside the right-hand end of the element are correctly spaced to form a promoter.
References
1] US Department of Energy Joint Genome Institute http://jgi.doe.gov/JGI_microbial/html/
2] Partridge and Hall (2003) J. Bacteriol. 185, 6371-6384
2] Partridge and Hall (2003) J. Bacteriol. 185, 6371-6384