ISCc3
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_002696 | ND | Caulobacter crescentus | Caulobacter crescentus CB15 |
DNA section
IS Length : 1514 bp
Ends
IR Length : 19/27
IRL : TGTCCGCCGTCAGCGCCAATGAGACAGATTGAGGGTTCCGTTTAGAGGAG
IRR : TGTACGTCGTCAGAAGTTTTGAGACAGCCGGGCTGCGGATTTAGTGGAGG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CTTTGGGCGA | TGTGTGACGATTTTG | CTGCAATCCA | 15 |
DNA sequence
TGTCCGCCGTCAGCGCCAATGAGACAGATTGAGGGTTCCGTTTAGAGGAGGGTTTTGGTCTCATCGAAGTGACGAAGGAACGAAGATGAGACCAAAACCC
TTGGCCGCGAAGGCGACGCCCGGTGAGCGGGTGGTGAAGGATATCCGGCGGGCGACGCGCCGGCATTTCTCGGCGGAAGACAAGATCCGGATCATACTGG
ACGGGCTGCGCGGGGAGGACAGCATTGCTGAGCTTTGCCGCAAGGAGGGCATCGCGCAGAGCCTGTATTACGTGTGGTCTAAGGAGTTCATGGAGGCGGG
CAAGCGCCGCCTGGCGGGCGATACGGCCCGAGCCGCGACGACGGACGAGGTCAAGGACCTGCGCCGAGAATCGACGGCCCTCAAAGAGGTGGTGGCCGAA
CAGGCGCTTGAAATCCGCCTGCTCAAAAAAAGCATGATCGCCGATGGGGGCGACGGGGAATGAGATATCCCGCTTGCGAAAAGATCGAGATCATCCGCCT
GGTCGAGCAATCGACCCTGCCCGTGCGCAGGACGCTGGCCCAGATCGGCGTGCCCCGCGCCACCTTCTATCGCTGGTATGATCTTTATCAGACCGGCGGT
CCGGAGGCGCTGGAAGACCGCCCGGCGCGCCCAAGCCGGGTGTGGAACCGCATCCCCGAGGATGTTCGCGCCGCGATCCTGGAGATGGCCCTGGAGCACT
CGGAGTTGAGCCCGCGGGAGCTGGCGGTGCGCTTCACCGACAGTCAGGGCTACTTCGTCTCGGAAGCCAGTGTTTACAGGCTTTTGAAGAGCCATGACCT
GATCACCAGCCCGGCCTTCATCGTCATGAAGGCCGCCAAGGCGTTCACCGACAAGACCACGGCGCCCAACCAGATGTGGCAGACTGATTTTACCTACTTC
AAGATCATCGGATGGGGCTGGGTCTATCTGTCGACGATCCTGGACGACTTCTCGCGCTACGTCATCGCCTGGAAGCTGTGCACGACCATGAAGGCGGGGG
ACGTCACCGATACGCTGGAACTGGCGCTCAGCGCGTCGGGCTGCGATCAGGTCAGCGTCCGGCACAAGCCGCGTCTGCTGTCGGACAATGGCTCCAGTTA
CATCTCGGGCGAACTGGCCGACTGGCTGGGGAAGCAGGAGATGAAACACGTCCGGGGCGCACCCTACCATCCCCAGACCCAGGGCAAGATCGAGCGCTGG
CATCAGACCCTGAAGAACCGCATCCTGCTGGAGAACTACTTCCTGCCCGGCGACCTGGAGGCCCAGATCGGCGCCTTCATCGAGCACTACAACCACGCCC
GCTATCACGAGAGCCTGGAGAATGTAACGCCGGCCGACGCCTACTTCGGCCGAGCCGCCGACATCCTGCGCCGCCGCGCCAGCGTCAAGCGACAGACCCT
TGAACATCGGCGCTTGCAGCACCGCAAAATCGCCGCCTAGATATCAAACCCAGACGAGGCCAACCCTCCACTAAATCCGCAGCCCGGCTGTCTCAAAACT
TCTGACGACGTACA
TTGGCCGCGAAGGCGACGCCCGGTGAGCGGGTGGTGAAGGATATCCGGCGGGCGACGCGCCGGCATTTCTCGGCGGAAGACAAGATCCGGATCATACTGG
ACGGGCTGCGCGGGGAGGACAGCATTGCTGAGCTTTGCCGCAAGGAGGGCATCGCGCAGAGCCTGTATTACGTGTGGTCTAAGGAGTTCATGGAGGCGGG
CAAGCGCCGCCTGGCGGGCGATACGGCCCGAGCCGCGACGACGGACGAGGTCAAGGACCTGCGCCGAGAATCGACGGCCCTCAAAGAGGTGGTGGCCGAA
CAGGCGCTTGAAATCCGCCTGCTCAAAAAAAGCATGATCGCCGATGGGGGCGACGGGGAATGAGATATCCCGCTTGCGAAAAGATCGAGATCATCCGCCT
GGTCGAGCAATCGACCCTGCCCGTGCGCAGGACGCTGGCCCAGATCGGCGTGCCCCGCGCCACCTTCTATCGCTGGTATGATCTTTATCAGACCGGCGGT
CCGGAGGCGCTGGAAGACCGCCCGGCGCGCCCAAGCCGGGTGTGGAACCGCATCCCCGAGGATGTTCGCGCCGCGATCCTGGAGATGGCCCTGGAGCACT
CGGAGTTGAGCCCGCGGGAGCTGGCGGTGCGCTTCACCGACAGTCAGGGCTACTTCGTCTCGGAAGCCAGTGTTTACAGGCTTTTGAAGAGCCATGACCT
GATCACCAGCCCGGCCTTCATCGTCATGAAGGCCGCCAAGGCGTTCACCGACAAGACCACGGCGCCCAACCAGATGTGGCAGACTGATTTTACCTACTTC
AAGATCATCGGATGGGGCTGGGTCTATCTGTCGACGATCCTGGACGACTTCTCGCGCTACGTCATCGCCTGGAAGCTGTGCACGACCATGAAGGCGGGGG
ACGTCACCGATACGCTGGAACTGGCGCTCAGCGCGTCGGGCTGCGATCAGGTCAGCGTCCGGCACAAGCCGCGTCTGCTGTCGGACAATGGCTCCAGTTA
CATCTCGGGCGAACTGGCCGACTGGCTGGGGAAGCAGGAGATGAAACACGTCCGGGGCGCACCCTACCATCCCCAGACCCAGGGCAAGATCGAGCGCTGG
CATCAGACCCTGAAGAACCGCATCCTGCTGGAGAACTACTTCCTGCCCGGCGACCTGGAGGCCCAGATCGGCGCCTTCATCGAGCACTACAACCACGCCC
GCTATCACGAGAGCCTGGAGAATGTAACGCCGGCCGACGCCTACTTCGGCCGAGCCGCCGACATCCTGCGCCGCCGCGCCAGCGTCAAGCGACAGACCCT
TGAACATCGGCGCTTGCAGCACCGCAAAATCGCCGCCTAGATATCAAACCCAGACGAGGCCAACCCTCCACTAAATCCGCAGCCCGGCTGTCTCAAAACT
TCTGACGACGTACA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 378 bp | 125 aa | 86 | 463 | + | No |
Description : First part of the transposase
ORF sequence :
MRPKPLAAKATPGERVVKDIRRATRRHFSAEDKIRIILDGLRGEDSIAELCRKEGIAQSLYYVWSKEFMEAGKRRLAGDTARAATTDEVKDLRRESTALK
EVVAEQALEIRLLKKSMIADGGDGE
EVVAEQALEIRLLKKSMIADGGDGE
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1029 bp | 342 aa | 412 | 1440 | + | No |
Description : Second part of the transposase
ORF sequence :
NPPAQKKHDRRWGRRGMRYPACEKIEIIRLVEQSTLPVRRTLAQIGVPRATFYRWYDLYQTGGPEALEDRPARPSRVWNRIPEDVRAAILEMALEHSELS
PRELAVRFTDSQGYFVSEASVYRLLKSHDLITSPAFIVMKAAKAFTDKTTAPNQMWQTDFTYFKIIGWGWVYLSTILDDFSRYVIAWKLCTTMKAGDVTD
TLELALSASGCDQVSVRHKPRLLSDNGSSYISGELADWLGKQEMKHVRGAPYHPQTQGKIERWHQTLKNRILLENYFLPGDLEAQIGAFIEHYNHARYHE
SLENVTPADAYFGRAADILRRRASVKRQTLEHRRLQHRKIAA
PRELAVRFTDSQGYFVSEASVYRLLKSHDLITSPAFIVMKAAKAFTDKTTAPNQMWQTDFTYFKIIGWGWVYLSTILDDFSRYVIAWKLCTTMKAGDVTD
TLELALSASGCDQVSVRHKPRLLSDNGSSYISGELADWLGKQEMKHVRGAPYHPQTQGKIERWHQTLKNRILLENYFLPGDLEAQIGAFIEHYNHARYHE
SLENVTPADAYFGRAADILRRRASVKRQTLEHRRLQHRKIAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1355 bp | 451 aa | 86 | 1440 | + | Yes |
Chemistry : DDE
ORF sequence :
MRPKPLAAKATPGERVVKDIRRATRRHFSAEDKIRIILDGLRGEDSIAELCRKEGIAQSLYYVWSKEFMEAGKRRLAGDTARAATTDEVKDLRRESTALK
EVVAEQALEIRLLKKKHDRRWGRRGMRYPACEKIEIIRLVEQSTLPVRRTLAQIGVPRATFYRWYDLYQTGGPEALEDRPARPSRVWNRIPEDVRAAILE
MALEHSELSPRELAVRFTDSQGYFVSEASVYRLLKSHDLITSPAFIVMKAAKAFTDKTTAPNQMWQTDFTYFKIIGWGWVYLSTILDDFSRYVIAWKLCT
TMKAGDVTDTLELALSASGCDQVSVRHKPRLLSDNGSSYISGELADWLGKQEMKHVRGAPYHPQTQGKIERWHQTLKNRILLENYFLPGDLEAQIGAFIE
HYNHARYHESLENVTPADAYFGRAADILRRRASVKRQTLEHRRLQHRKIAA
EVVAEQALEIRLLKKKHDRRWGRRGMRYPACEKIEIIRLVEQSTLPVRRTLAQIGVPRATFYRWYDLYQTGGPEALEDRPARPSRVWNRIPEDVRAAILE
MALEHSELSPRELAVRFTDSQGYFVSEASVYRLLKSHDLITSPAFIVMKAAKAFTDKTTAPNQMWQTDFTYFKIIGWGWVYLSTILDDFSRYVIAWKLCT
TMKAGDVTDTLELALSASGCDQVSVRHKPRLLSDNGSSYISGELADWLGKQEMKHVRGAPYHPQTQGKIERWHQTLKNRILLENYFLPGDLEAQIGAFIE
HYNHARYHESLENVTPADAYFGRAADILRRRASVKRQTLEHRRLQHRKIAA
Blast result :
Comments
There are three copies of ISCc3 in this strain, and two of them lack a direct repeat.
ISCc3 is 56% (ORF1) and 48% (ORF2) aa similar to IS1302.
ISCc3 is 56% (ORF1) and 48% (ORF2) aa similar to IS1302.
References
1] Nierman, W.C., Feldblyum, T.V., Laub, M.T., Paulsen, I.T., Nelson, K.E., Eisen, J., Heidelberg, J.F., Alley, M.R.K., Ohta, N., Maddock, J.R., Potocka, I., Nelson, W.C., Newton, A., Stephens, C., Phadke, N.D., Ely, B., DeBoy, R.T., Dodson, R.J., Durkin, A.S., Gwinn, M.L., Haft, D.H., Kolonay, J.F., Smit, J., Craven, M.B., Khouri, H., Shetty, J., Berry, K., Utterback, T., Tran, K., Wolf, A., Vamathevan, J., Ermolaeva, M., White, O., Salzberg, S.L., Venter, J.C., Shapiro, L. and Fraser, C.M. PNAS 98: 4136-4141 (2001)