ISAba2
- Family IS3
- Group IS51
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AY665723 | ND | Acinetobacter baumannii | Acinetobacter baumannii MAD |
DNA section
IS Length : 1308 bp
Ends
IR Length : 26
IRL : TGAACCGTACCGGGTTTGTCGGAGACCAACTTTCTTGAGAGAATGTCCCG
IRR : TGAACCGTACCGGGTTTGTCGGAGACTTTTTATTTAAGTTAGGCCACCTG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AAATGGTATCA | AAG | TGATGGGAAA | 3 |
DNA sequence
TGAACCGTACCGGGTTTGTCGGAGACCAACTTTCTTGAGAGAATGTCCCGATGACAAAACCAAAATATACCCCTGAAATCCGAGATAGAGCGGTTCAATT
ATTGATTGAATCCGAAAAAGATTATCCATCGAATTGGGCTGCAATCACTGCTATTGCTCCCAAGATAGGTTGTACTCCTGAAACACTGCGTGTTTGGTAT
TTAAAACATCTCGATCAACAAAATCCTATCAAAGTACAACAGGCATCAGATCAAGAAAAAATGAAGCAGATGGAACGTGAAATCAAAGAATTAAAGCGTG
CCAATGAAATTCTACGTAAAGCAGCTGCTTTTTTCGCCCAGGCGGAGCTCGACCGCCCACACAAATAATGGTGGATTTCATCCATAACAATAAAGACTTA
TATGGTGTTGATGCGATTTGTAGGATTTTACCGATCGCAGCTTCAACCTATTACCGAACTTTAGATCTCGCGGACAATCCAGAACATCGAGCGAAGCGAG
ATTTACATGACTTGCATCATGCTGAACAAATTAAACGAATTTGGAAGGAAAGTTCAGGTCGATATGGTGTACGTAAAGTTTGGCAAAAACTGAAACGTGA
AGGCTATATTATTGCACGCTGTACAGTTGCTCGATTGATGCAAAAGCTAGGTATACAAGGTGTTTGGCGAGGTAAGAATAAACAAACTACCCATAGCCGA
GATGATCAAAAACGAGCAGATGACTTGGTGAAACGCAATTTTAGTGCTGATCACCCTGACCAGCTGTGGGTCGCTGACTTTACGTATATTCAAACAAATT
CAGGCTGGGTCTATACCGCCTTTATTATTGATGTGTTCTCGCGAGCAATTGTTGGATGGAAAGTATCAACACGGATGAATACAGACATGGTGCTCGATGC
ACTGGAGCAAGCATTGCATGATCGAGGCATGCCAAAGAACGTGATTCATCATAGTGACAGAGGTGTGCAATATCTTTCCATTCGTTATACCAATCGTTTA
GAAGCAGCAAATTTACGAGCATCAGTCGGTACGACCGGTGATTCATACGATAATGCTTTGGCTGAAACGGTGAATGGCTTATACAAAACAGAGGTGATTG
AATATTTAAAAGCGGATTGGCAAGGTTTAGCGGATGTACAACTTGCGACATTAAATTGGGTAGATTGGTTCAATAAAGAGCGTGTACATAGTGCACTAGG
TTATGTGTCACCTTTTGATTTTGAAGCAATGTACTATGATAAAATTAACCCGTTAGGTCAGGTGGCCTAACTTAAATAAAAAGTCTCCGACAAACCCGGT
ACGGTTCA
ATTGATTGAATCCGAAAAAGATTATCCATCGAATTGGGCTGCAATCACTGCTATTGCTCCCAAGATAGGTTGTACTCCTGAAACACTGCGTGTTTGGTAT
TTAAAACATCTCGATCAACAAAATCCTATCAAAGTACAACAGGCATCAGATCAAGAAAAAATGAAGCAGATGGAACGTGAAATCAAAGAATTAAAGCGTG
CCAATGAAATTCTACGTAAAGCAGCTGCTTTTTTCGCCCAGGCGGAGCTCGACCGCCCACACAAATAATGGTGGATTTCATCCATAACAATAAAGACTTA
TATGGTGTTGATGCGATTTGTAGGATTTTACCGATCGCAGCTTCAACCTATTACCGAACTTTAGATCTCGCGGACAATCCAGAACATCGAGCGAAGCGAG
ATTTACATGACTTGCATCATGCTGAACAAATTAAACGAATTTGGAAGGAAAGTTCAGGTCGATATGGTGTACGTAAAGTTTGGCAAAAACTGAAACGTGA
AGGCTATATTATTGCACGCTGTACAGTTGCTCGATTGATGCAAAAGCTAGGTATACAAGGTGTTTGGCGAGGTAAGAATAAACAAACTACCCATAGCCGA
GATGATCAAAAACGAGCAGATGACTTGGTGAAACGCAATTTTAGTGCTGATCACCCTGACCAGCTGTGGGTCGCTGACTTTACGTATATTCAAACAAATT
CAGGCTGGGTCTATACCGCCTTTATTATTGATGTGTTCTCGCGAGCAATTGTTGGATGGAAAGTATCAACACGGATGAATACAGACATGGTGCTCGATGC
ACTGGAGCAAGCATTGCATGATCGAGGCATGCCAAAGAACGTGATTCATCATAGTGACAGAGGTGTGCAATATCTTTCCATTCGTTATACCAATCGTTTA
GAAGCAGCAAATTTACGAGCATCAGTCGGTACGACCGGTGATTCATACGATAATGCTTTGGCTGAAACGGTGAATGGCTTATACAAAACAGAGGTGATTG
AATATTTAAAAGCGGATTGGCAAGGTTTAGCGGATGTACAACTTGCGACATTAAATTGGGTAGATTGGTTCAATAAAGAGCGTGTACATAGTGCACTAGG
TTATGTGTCACCTTTTGATTTTGAAGCAATGTACTATGATAAAATTAACCCGTTAGGTCAGGTGGCCTAACTTAAATAAAAAGTCTCCGACAAACCCGGT
ACGGTTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 318 bp | 105 aa | 51 | 368 | + | No |
Description : First part of the transposase
ORF sequence :
MTKPKYTPEIRDRAVQLLIESEKDYPSNWAAITAIAPKIGCTPETLRVWYLKHLDQQNPIKVQQASDQEKMKQMEREIKELKRANEILRKAAAFFAQAEL
DRPHK
DRPHK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 951 bp | 316 aa | 320 | 1270 | + | No |
Description : Second part of the transposase
ORF sequence :
SSCFFRPGGARPPTQIMVDFIHNNKDLYGVDAICRILPIAASTYYRTLDLADNPEHRAKRDLHDLHHAEQIKRIWKESSGRYGVRKVWQKLKREGYIIAR
CTVARLMQKLGIQGVWRGKNKQTTHSRDDQKRADDLVKRNFSADHPDQLWVADFTYIQTNSGWVYTAFIIDVFSRAIVGWKVSTRMNTDMVLDALEQALH
DRGMPKNVIHHSDRGVQYLSIRYTNRLEAANLRASVGTTGDSYDNALAETVNGLYKTEVIEYLKADWQGLADVQLATLNWVDWFNKERVHSALGYVSPFD
FEAMYYDKINPLGQVA
CTVARLMQKLGIQGVWRGKNKQTTHSRDDQKRADDLVKRNFSADHPDQLWVADFTYIQTNSGWVYTAFIIDVFSRAIVGWKVSTRMNTDMVLDALEQALH
DRGMPKNVIHHSDRGVQYLSIRYTNRLEAANLRASVGTTGDSYDNALAETVNGLYKTEVIEYLKADWQGLADVQLATLNWVDWFNKERVHSALGYVSPFD
FEAMYYDKINPLGQVA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1220 bp | 406 aa | 51 | 1270 | + | Yes |
Chemistry : DDE
ORF sequence :
MTKPKYTPEIRDRAVQLLIESEKDYPSNWAAITAIAPKIGCTPETLRVWYLKHLDQQNPIKVQQASDQEKMKQMEREIKELKRANEILRKAAAFFRPGGA
RPPTQIMVDFIHNNKDLYGVDAICRILPIAASTYYRTLDLADNPEHRAKRDLHDLHHAEQIKRIWKESSGRYGVRKVWQKLKREGYIIARCTVARLMQKL
GIQGVWRGKNKQTTHSRDDQKRADDLVKRNFSADHPDQLWVADFTYIQTNSGWVYTAFIIDVFSRAIVGWKVSTRMNTDMVLDALEQALHDRGMPKNVIH
HSDRGVQYLSIRYTNRLEAANLRASVGTTGDSYDNALAETVNGLYKTEVIEYLKADWQGLADVQLATLNWVDWFNKERVHSALGYVSPFDFEAMYYDKIN
PLGQVA
RPPTQIMVDFIHNNKDLYGVDAICRILPIAASTYYRTLDLADNPEHRAKRDLHDLHHAEQIKRIWKESSGRYGVRKVWQKLKREGYIIARCTVARLMQKL
GIQGVWRGKNKQTTHSRDDQKRADDLVKRNFSADHPDQLWVADFTYIQTNSGWVYTAFIIDVFSRAIVGWKVSTRMNTDMVLDALEQALHDRGMPKNVIH
HSDRGVQYLSIRYTNRLEAANLRASVGTTGDSYDNALAETVNGLYKTEVIEYLKADWQGLADVQLATLNWVDWFNKERVHSALGYVSPFDFEAMYYDKIN
PLGQVA
Blast result :
Comments
ISAba2 ORF1 is 74% aa similar to IS51 (ORF1) and ORF2 66% to IS51 (ORF2).
References
1] Laurent Poirel, (2004) Direct submission
2] Poirel L, Nordmann P (2006). Antimicrob. Agents Chemother., 50 :1442-1448.
2] Poirel L, Nordmann P (2006). Antimicrob. Agents Chemother., 50 :1442-1448.